Rhodococcus zopfii T-1 is an obligate aerobe, Gram-positive, rod-shaped bacterium that was isolated from bioreactor.
Gram-positive rod-shaped obligate aerobe genome sequence 16S sequence Bacteria| @ref 20215 |
|
|
| Domain Bacteria |
| Phylum Actinomycetota |
| Class Actinomycetes |
| Order Mycobacteriales |
| Family Nocardiaceae |
| Genus Rhodococcus |
| Species Rhodococcus zopfii |
| Full scientific name Rhodococcus zopfii Stoecker et al. 1994 |
| BacDive ID | Other strains from Rhodococcus zopfii (2) | Type strain |
|---|---|---|
| 11025 | R. zopfii Tue Acy 3, DSM 44189 | |
| 103562 | R. zopfii ST033350(HKI), |
| @ref: | 11403 |
| multimedia content: | DSM_44108.jpg |
| multimedia.multimedia content: | https://www.dsmz.de/microorganisms/photos/DSM_44108.jpg |
| caption: | Medium 215 28°C |
| intellectual property rights: | © Leibniz-Institut DSMZ |
| manual_annotation: | 1 |
| @ref | Name | Growth | Medium link | Composition | |
|---|---|---|---|---|---|
| 11403 | BHI MEDIUM (DSMZ Medium 215) | Medium recipe at MediaDive | Name: BHI MEDIUM (DSMZ Medium 215) Composition: Brain heart infusion 37.0 g/l Distilled water | ||
| 11403 | GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) | Medium recipe at MediaDive | Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 18.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water | ||
| 19999 | ISP 2 | Name: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes | |||
| 19999 | ISP 3 | Name: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms: | |||
| 19999 | ISP 4 | Name: ISP 4; DSM 547 Solution I: Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml. Solution II: CaCO3 2.0 g K2HPO4 (anhydrous) 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g (NH4)2SO4 2.0 g Distilled water 500.0 ml Trace salt solution (see below) 1.0 ml The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min. Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml | |||
| 19999 | ISP 5 | Name: ISP 5 (5323) Composition L-Asparagine 1.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l Salt solution (see preparation) 1.0 ml/l Agar 20.0 g/l Preparation: Salt solution 1.0 g FeSO4 x 7 H2O 1.0 g MnCl2 x 4 H2O 1.0 g ZNSO4 x 7 H2O in 100 ml water Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.2 Usage: Maintenance and taxonomy Organisms: All Actinomycetes | |||
| 19999 | ISP 6 | Name: ISP 6 (5318) Composition Peptone 15.0 g/l Proteose peptose 5.0 g/l Ferric ammonium citrate 0.5 g/l Sodium glycerophosphate 1.0 g/l Sodium thiosulfate 0.08 g/l Yeast extract 1.0 g/l Agar 15.0 g/l Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Production of melanoid pigments Organisms: All Actinomycetes | |||
| 19999 | ISP 7 | Name: ISP 7 (5322) Composition Glycerol 15.0 g/l L-Tyrosine 0.5 g/l L-Asparagine 1.0 g/l K2HPO4 0.5 g/l NaCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Trace element solution 5343 1.0 ml/l Agar 20.0 Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.3 Usage: Production of melanoid pigments Organisms: All Actinomycetes | |||
| 41082 | MEDIUM 3 - Columbia agar | Columbia agar (39.000 g);distilled water (1000.000 ml) | |||
| 124038 | CIP Medium 3 | Medium recipe at CIP |
| 19999 | Spore formationno |
| 20216 | ObservationDegrades toluene and other benzene derivatives |
| @ref | Chebi-ID | Metabolite | Utilization activity | Kind of utilization tested | |
|---|---|---|---|---|---|
| 68371 | 27613 ChEBI | amygdalin | - | builds acid from | from API 50CH acid |
| 19999 | 22599 ChEBI | arabinose | + | ||
| 68371 | 18305 ChEBI | arbutin | - | builds acid from | from API 50CH acid |
| 68368 | 29016 ChEBI | arginine | + | hydrolysis | from API 20E |
| 68371 | 17057 ChEBI | cellobiose | - | builds acid from | from API 50CH acid |
| 19999 | 62968 ChEBI | cellulose | + | ||
| 124038 | 16947 ChEBI | citrate | - | carbon source | |
| 68368 | 16947 ChEBI | citrate | + | assimilation | from API 20E |
| 68371 | 18333 ChEBI | D-arabitol | - | builds acid from | from API 50CH acid |
| 68371 | 15824 ChEBI | D-fructose | + | builds acid from | from API 50CH acid |
| 68371 | 28847 ChEBI | D-fucose | - | builds acid from | from API 50CH acid |
| 68371 | 12936 ChEBI | D-galactose | - | builds acid from | from API 50CH acid |
| 68371 | 17634 ChEBI | D-glucose | + | builds acid from | from API 50CH acid |
| 68371 | 62318 ChEBI | D-lyxose | - | builds acid from | from API 50CH acid |
| 68371 | 16024 ChEBI | D-mannose | - | builds acid from | from API 50CH acid |
| 68371 | 16988 ChEBI | D-ribose | - | builds acid from | from API 50CH acid |
| 68371 | 16443 ChEBI | D-tagatose | - | builds acid from | from API 50CH acid |
| 68371 | 65327 ChEBI | D-xylose | - | builds acid from | from API 50CH acid |
| 68371 | 17113 ChEBI | erythritol | - | builds acid from | from API 50CH acid |
| 124038 | 4853 ChEBI | esculin | - | hydrolysis | |
| 68371 | 4853 ChEBI | esculin | + | builds acid from | from API 50CH acid |
| 19999 | 28757 ChEBI | fructose | + | ||
| 68368 | 5291 ChEBI | gelatin | - | hydrolysis | from API 20E |
| 68371 | 28066 ChEBI | gentiobiose | - | builds acid from | from API 50CH acid |
| 68371 | 24265 ChEBI | gluconate | - | builds acid from | from API 50CH acid |
| 19999 | 17234 ChEBI | glucose | + | ||
| 124038 | 17234 ChEBI | glucose | - | fermentation | |
| 68371 | 17754 ChEBI | glycerol | - | builds acid from | from API 50CH acid |
| 68371 | 28087 ChEBI | glycogen | - | builds acid from | from API 50CH acid |
| 124038 | 606565 ChEBI | hippurate | - | hydrolysis | |
| 68371 | 15443 ChEBI | inulin | - | builds acid from | from API 50CH acid |
| 68371 | 30849 ChEBI | L-arabinose | - | builds acid from | from API 50CH acid |
| 68371 | 18403 ChEBI | L-arabitol | - | builds acid from | from API 50CH acid |
| 68371 | 18287 ChEBI | L-fucose | - | builds acid from | from API 50CH acid |
| 68371 | 17266 ChEBI | L-sorbose | - | builds acid from | from API 50CH acid |
| 68371 | 65328 ChEBI | L-xylose | - | builds acid from | from API 50CH acid |
| 124038 | 17716 ChEBI | lactose | - | fermentation | |
| 68371 | 17716 ChEBI | lactose | - | builds acid from | from API 50CH acid |
| 68368 | 25094 ChEBI | lysine | + | degradation | from API 20E |
| 68371 | 17306 ChEBI | maltose | - | builds acid from | from API 50CH acid |
| 19999 | 29864 ChEBI | mannitol | + | ||
| 68371 | 6731 ChEBI | melezitose | - | builds acid from | from API 50CH acid |
| 68371 | 43943 ChEBI | methyl alpha-D-mannoside | - | builds acid from | from API 50CH acid |
| 68371 | 74863 ChEBI | methyl beta-D-xylopyranoside | - | builds acid from | from API 50CH acid |
| 19999 | 17268 ChEBI | myo-inositol | + | ||
| 124038 | 17632 ChEBI | nitrate | + | reduction | |
| 124038 | 17632 ChEBI | nitrate | - | respiration | |
| 124038 | 16301 ChEBI | nitrite | - | reduction | |
| 68368 | 18257 ChEBI | ornithine | - | degradation | from API 20E |
| 68371 | Potassium 2-ketogluconate | - | builds acid from | from API 50CH acid | |
| 68371 | Potassium 5-ketogluconate | - | builds acid from | from API 50CH acid | |
| 19999 | 16634 ChEBI | raffinose | + | ||
| 68371 | 16634 ChEBI | raffinose | - | builds acid from | from API 50CH acid |
| 19999 | 26546 ChEBI | rhamnose | + | ||
| 68371 | 15963 ChEBI | ribitol | - | builds acid from | from API 50CH acid |
| 68371 | 17814 ChEBI | salicin | - | builds acid from | from API 50CH acid |
| 124038 | 132112 ChEBI | sodium thiosulfate | - | builds gas from | |
| 68371 | 28017 ChEBI | starch | - | builds acid from | from API 50CH acid |
| 19999 | 17992 ChEBI | sucrose | + | ||
| 68368 | 27897 ChEBI | tryptophan | - | energy source | from API 20E |
| 68371 | 32528 ChEBI | turanose | - | builds acid from | from API 50CH acid |
| 68368 | 16199 ChEBI | urea | + | hydrolysis | from API 20E |
| 19999 | 18222 ChEBI | xylose | + |
| @ref | Value | Activity | Ec | |
|---|---|---|---|---|
| 124038 | alcohol dehydrogenase | - | 1.1.1.1 | |
| 68382 | alpha-chymotrypsin | - | 3.4.21.1 | from API zym |
| 68382 | alpha-fucosidase | - | 3.2.1.51 | from API zym |
| 68382 | alpha-galactosidase | - | 3.2.1.22 | from API zym |
| 68382 | alpha-glucosidase | - | 3.2.1.20 | from API zym |
| 68382 | alpha-mannosidase | - | 3.2.1.24 | from API zym |
| 124038 | amylase | - | ||
| 68368 | arginine dihydrolase | + | 3.5.3.6 | from API 20E |
| 68382 | beta-galactosidase | - | 3.2.1.23 | from API zym |
| 124038 | beta-galactosidase | - | 3.2.1.23 | |
| 68368 | beta-galactosidase | + | 3.2.1.23 | from API 20E |
| 68382 | beta-glucosidase | + | 3.2.1.21 | from API zym |
| 68382 | beta-glucuronidase | - | 3.2.1.31 | from API zym |
| 124038 | caseinase | - | 3.4.21.50 | |
| 124038 | catalase | + | 1.11.1.6 | |
| 124038 | DNase | - | ||
| 68382 | esterase lipase (C 8) | + | from API zym | |
| 124038 | gamma-glutamyltransferase | - | 2.3.2.2 | |
| 124038 | gelatinase | - | ||
| 68368 | gelatinase | - | from API 20E | |
| 124038 | lecithinase | - | ||
| 124038 | lipase | - | ||
| 68382 | lipase (C 14) | - | from API zym | |
| 124038 | lysine decarboxylase | - | 4.1.1.18 | |
| 68368 | lysine decarboxylase | + | 4.1.1.18 | from API 20E |
| 68382 | N-acetyl-beta-glucosaminidase | - | 3.2.1.52 | from API zym |
| 68382 | naphthol-AS-BI-phosphohydrolase | - | from API zym | |
| 124038 | ornithine decarboxylase | - | 4.1.1.17 | |
| 68368 | ornithine decarboxylase | - | 4.1.1.17 | from API 20E |
| 124038 | oxidase | - | ||
| 124038 | phenylalanine ammonia-lyase | + | 4.3.1.24 | |
| 124038 | protease | - | ||
| 124038 | tryptophan deaminase | - | ||
| 68368 | tryptophan deaminase | - | 4.1.99.1 | from API 20E |
| 124038 | tween esterase | - | ||
| 124038 | urease | + | 3.5.1.5 | |
| 68368 | urease | + | 3.5.1.5 | from API 20E |
| @ref | ControlQ | GLY | ERY | DARA | LARA | RIB | DXYL | LXYL | ADO | MDX | GAL | GLU | FRU | MNE | SBE | RHA | DUL | INO | MAN | SOR | MDM | MDG | NAG | AMY | ARB | ESC | SAL | CEL | MAL | LAC | MEL | SAC | TRE | INU | MLZ | RAF | AMD | GLYG | XLT | GEN | TUR | LYX | TAG | DFUC | LFUC | DARL | LARL | GNT | 2KG | 5KG | |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 124038 | not determinedn.d. | - | - | +/- | - | - | - | - | - | - | - | + | + | - | - | +/- | +/- | +/- | +/- | +/- | - | +/- | +/- | - | - | + | - | - | - | - | +/- | +/- | +/- | - | - | - | - | - | +/- | - | - | - | - | - | - | - | - | - | - | - |
Global distribution of 16S sequence X81934 (>99% sequence identity) for Rhodococcus from Microbeatlas ![]()
| @ref | Description | Assembly level | INSDC accession | BV-BRC accession | IMG accession | NCBI tax ID | Score | |
|---|---|---|---|---|---|---|---|---|
| 67770 | ASM189502v1 assembly for Rhodococcus zopfii NBRC 100606 = JCM 9919 | contig | 1219030 | 48.86 | ||||
| 66792 | ASM131370v1 assembly for Rhodococcus zopfii NBRC 100606 = JCM 9919 | contig | 1219030 | |||||
| 66792 | ASM2213473v1 assembly for Rhodococcus zopfii ATCC 51349 | contig | 43772 |
| @ref | Description | Accession | Length | Database | NCBI tax ID | |
|---|---|---|---|---|---|---|
| 20218 | Rhodococcus zopfii 16S ribosomal RNA gene, partial sequence | AF191343 | 1478 | 43772 | ||
| 20218 | R.zopfii 16S rRNA gene (ATCC 51349T) | X81934 | 1319 | 43772 | ||
| 124043 | Rhodococcus zopfii strain DSM 44108 16S ribosomal RNA gene, partial sequence. | KF410350 | 1371 | 43772 | ||
| 124043 | Rhodococcus zopfii strain ATCC 51349 16S ribosomal RNA gene, partial sequence. | MK775203 | 1306 | 43772 | ||
| 124043 | Rhodococcus zopfii strain NBRC 100606(T) 16S ribosomal RNA gene, partial sequence. | MH236121 | 806 | 43772 | ||
| 124043 | Rhodococcus zopfii strain NBRC 100606(T) 16S ribosomal RNA gene, partial sequence. | MH193361 | 706 | 43772 |
| @ref | Trait | Model | Prediction | Confidence in % | In training data |
|---|---|---|---|---|---|
| 125439 | oxygen_tolerance | BacteriaNetⓘ | obligate aerobe | 99.20 | no |
| 125439 | gram_stain | BacteriaNetⓘ | positive | 99.20 | no |
| 125439 | motility | BacteriaNetⓘ | no | 91.80 | no |
| 125439 | spore_formation | BacteriaNetⓘ | yes | 69.90 | no |
| @ref | Trait | Model | Prediction | Confidence in % | In training data |
|---|---|---|---|---|---|
| 125438 | gram-positive | gram-positiveⓘ | yes | 91.47 | no |
| 125438 | anaerobic | anaerobicⓘ | no | 97.53 | yes |
| 125438 | spore-forming | spore-formingⓘ | no | 63.51 | yes |
| 125438 | aerobic | aerobicⓘ | yes | 89.25 | no |
| 125438 | thermophilic | thermophileⓘ | no | 96.50 | no |
| 125438 | flagellated | motile2+ⓘ | no | 92.50 | no |
| Topic | Title | Authors | Journal | DOI | Year | |
|---|---|---|---|---|---|---|
| UV-LED Combined with Small Bioreactor Platform (SBP) for Degradation of 17alpha-Ethynylestradiol (EE2) at Very Short Hydraulic Retention Time. | Fradkin O, Mamane H, Kaplan A, Menashe O, Kurzbaum E, Betzalel Y, Avisar D. | Materials (Basel) | 10.3390/ma14205960 | 2021 | ||
| LP-UV-Nano MgO2 Pretreated Catalysis Followed by Small Bioreactor Platform Capsules Treatment for Superior Kinetic Degradation Performance of 17alpha-Ethynylestradiol. | Vaddadi LP, Avisar D, Vadivel VK, Menashe O, Kurzbaum E, Cohen-Yaniv V, Mamane H. | Materials (Basel) | 10.3390/ma13010083 | 2019 | ||
| Metabolism | Isolation, Characterization, and Degradation Performance of the 17beta-Estradiol-Degrading Bacterium Novosphingobium sp. E2S. | Li S, Liu J, Sun M, Ling W, Zhu X. | Int J Environ Res Public Health | 10.3390/ijerph14020115 | 2017 | |
| Pathogenicity | Relationships between colony morphotypes and oil tolerance in Rhodococcus rhodochrous. | Iwabuchi N, Sunairi M, Anzai H, Nakajima M, Harayama S. | Appl Environ Microbiol | 10.1128/aem.66.11.5073-5077.2000 | 2000 | |
| Phylogeny | Rhodococcus phenolicus sp. nov., a novel bioprocessor isolated actinomycete with the ability to degrade chlorobenzene, dichlorobenzene and phenol as sole carbon sources. | Rehfuss M, Urban J | Syst Appl Microbiol | 10.1016/j.syapm.2005.05.011 | 2005 | |
| Phylogeny | Rhodococcus yananensis sp. nov., a novel denitrification actinobacterium isolated from microbial fermentation bed material from a pig farm. | Jiang Y, Zheng C, Yu T, Li J, Ai J, Li M, Liu X, Deng Z | Int J Syst Evol Microbiol | 10.1099/ijsem.0.005426 | 2022 |
| #11403 | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 44108 |
| #19999 | Wink, J.: Compendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig . |
| #20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 ) |
| #20216 | Curators of the HKI: Collection Description Leibniz-Institut für Naturstoff-Forschung und Infektionsbiologie e. V. Hans-Knöll-Institut (HKI) . Leibniz-Institut für Naturstoff-Forschung und Infektionsbiologie e. V. Hans-Knöll-Institut (HKI): |
| #20218 | Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 ) |
| #41082 | ; Curators of the CIP; |
| #66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) . |
| #67770 | Japan Collection of Microorganism (JCM) ; Curators of the JCM; |
| #68368 | Automatically annotated from API 20E . |
| #68371 | Automatically annotated from API 50CH acid . |
| #68382 | Automatically annotated from API zym . |
| #69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta . |
| #124038 | Collection of Institut Pasteur ; Curators of the CIP; CIP 104275 |
| #124043 | Isabel Schober, Julia Koblitz: Data extracted from sequence databases, automatically matched based on designation and taxonomy . |
| #125438 | Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 ) |
| #125439 | Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 . |
| #126262 | A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 ) |
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