Nocardia jejuensis N 3-2, N3-2 | Type strain | DSM 44959, JCM 13281, NRRL B-24430, CIP 109185, IFM 10687, NBRC 103114, NCIMB 14343

Caption:

(ISP6, ISP7, SSM+T, SSM-T)

Intellectual property rights:

Helmholtz-Zentrum für Infektionsforschung GmbH

Caption:

(ISP6, ISP7, SSM+T, SSM-T)

Intellectual property rights:

Helmholtz-Zentrum für Infektionsforschung GmbH

Information on culture and growth conditions Culture and growth conditions

Culture medium

TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535)

Culture medium growth

Medium recipe at MediaDive

Culture medium link

Culture medium composition

expand / minimize

Culture medium

GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)

Culture medium growth

Medium recipe at MediaDive

Culture medium link

Culture medium composition

expand / minimize

[Ref.: #37473]

Culture medium

MEDIUM 72- for trypto casein soja agar

[Ref.: #37473]

Culture medium growth

[Ref.: #37473]

Culture medium composition

expand / minimize

Culture medium

CIP Medium 72

Culture medium growth

Medium recipe provided by DSMZ

Culture medium link

Culture medium

CIP Medium 115

Culture medium growth

Medium recipe provided by DSMZ

Culture medium link

		Temperature
[Ref.: #12212]	growth	28 °C
[Ref.: #19894]	optimum	28 °C
[Ref.: #31595]	growth	10-37 °C
[Ref.: #31595]	optimum	30-37 °C
[Ref.: #37473]	growth	30 °C
[Ref.: #67770]	growth	28 °C

[Ref.: #12212]	Temperature range	mesophilic
[Ref.: #19894]	Temperature range	mesophilic
[Ref.: #31595]	Temperature range	mesophilic
[Ref.: #37473]	Temperature range	mesophilic
[Ref.: #67770]	Temperature range	mesophilic

	pH	Kind of pH		pH
[Ref.: #31595]			optimum	7.1-8.1
[Ref.: #31595]			growth	4.1-10.1

Information on physiology and metabolism Physiology and metabolism

[Ref.: #12212]	Oxygen tolerance	aerobe
[Ref.: #31595]	Oxygen tolerance	aerobe
[Ref.: #120327]	Oxygen tolerance	obligate aerobe

[Ref.: #31595]	Ability of spore formation	yes
[Ref.: #69481]	Ability of spore formation	yes Confidence in %100
[Ref.: #69480]	Ability of spore formation	no Confidence in %91.042

	Salt		Salt conc.
[Ref.: #31595]	NaCl	growth	0-4	%
[Ref.: #31595]	NaCl	optimum	0-4	%
[Ref.: #69369]	NaCl	growth	0-2.5	%

[Ref.: #31595]	Observation	aggregates in chains
[Ref.: #67770]	Observation	quinones: MK-8(H₄, ω-cycl)

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #68371]	amygdalin ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #69369]	arabinose ChEBI	-	growth
[Ref.: #31595]	arabinose ChEBI	+	carbon source
[Ref.: #68371]	arbutin ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	cellobiose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #69369]	cellulose ChEBI	-	growth
[Ref.: #68371]	D-arabinose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-arabitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-fructose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-fucose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-galactose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-glucose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68379]	D-glucose ChEBI	-	fermentation	* from API Coryne
[Ref.: #68371]	D-lyxose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-mannitol ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68379]	D-mannitol ChEBI	-	fermentation	* from API Coryne
[Ref.: #68371]	D-mannose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-ribose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68379]	D-ribose ChEBI	-	fermentation	* from API Coryne
[Ref.: #68371]	D-sorbitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-tagatose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	D-xylose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68379]	D-xylose ChEBI	-	fermentation	* from API Coryne
[Ref.: #68371]	erythritol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #31595]	esculin ChEBI	+	hydrolysis
[Ref.: #68371]	esculin ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #31595]	fructose ChEBI	+	carbon source
[Ref.: #69369]	fructose ChEBI	-	growth
[Ref.: #68371]	galactitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68379]	gelatin ChEBI	-	hydrolysis	* from API Coryne
[Ref.: #68371]	gentiobiose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #31595]	glucose ChEBI	+	carbon source
[Ref.: #69369]	glucose ChEBI	+	growth
[Ref.: #31595]	glycerol ChEBI	+	carbon source
[Ref.: #68371]	glycerol ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	glycogen ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68379]	glycogen ChEBI	-	fermentation	* from API Coryne
[Ref.: #69369]	inositol ChEBI	+	growth
[Ref.: #68371]	inulin ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-arabinose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-arabitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-fucose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-rhamnose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-sorbose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	L-xylose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #31595]	lactose ChEBI	+	carbon source
[Ref.: #68371]	lactose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68379]	lactose ChEBI	-	fermentation	* from API Coryne
[Ref.: #68371]	maltose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68379]	maltose ChEBI	-	fermentation	* from API Coryne
[Ref.: #69369]	mannose ChEBI	-	growth
[Ref.: #68371]	melezitose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #31595]	melibiose ChEBI	+	carbon source
[Ref.: #68371]	melibiose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	methyl alpha-D-glucopyranoside ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	methyl beta-D-xylopyranoside ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	myo-inositol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	N-acetylglucosamine ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #31595]	nitrate ChEBI	+	reduction
[Ref.: #120327]	nitrate ChEBI	+	reduction
[Ref.: #68379]	nitrate ChEBI	+	reduction	* from API Coryne
[Ref.: #120327]	nitrite ChEBI	-	reduction
[Ref.: #68371]	Potassium 2-ketogluconate ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #68371]	Potassium 5-ketogluconate ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #69369]	raffinose ChEBI	-	growth
[Ref.: #68371]	raffinose ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #69369]	rhamnose ChEBI	-	growth
[Ref.: #68371]	ribitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #31595]	ribose ChEBI	+	carbon source
[Ref.: #31595]	salicin ChEBI	+	carbon source
[Ref.: #68371]	salicin ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #31595]	sorbitol ChEBI	+	carbon source
[Ref.: #68371]	starch ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #31595]	sucrose ChEBI	+	carbon source
[Ref.: #69369]	sucrose ChEBI	-	growth
[Ref.: #68371]	sucrose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68379]	sucrose ChEBI	-	fermentation	* from API Coryne
[Ref.: #68371]	trehalose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	turanose ChEBI	+	builds acid from	* from API 50CH acid
[Ref.: #68371]	xylitol ChEBI	-	builds acid from	* from API 50CH acid
[Ref.: #69369]	xylose ChEBI	-	growth
	Only first 10 entries are displayed. Click here to see all.

	Metabolite production	Metabolite	Production
[Ref.: #120327]		indole ChEBI	no

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	+	3.1.3.2	* from API zym
[Ref.: #68379]	alkaline phosphatase	+	3.1.3.1	* from API Coryne, API zym
[Ref.: #68382]	alpha-chymotrypsin	-	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	-	3.2.1.22	* from API zym
[Ref.: #68379]	alpha-glucosidase	+	3.2.1.20	* from API Coryne, API zym
[Ref.: #68379]	beta-galactosidase	-	3.2.1.23	* from API Coryne
[Ref.: #68382]	beta-glucosidase	+	3.2.1.21	* from API zym
[Ref.: #68379]	beta-glucuronidase	-	3.2.1.31	* from API Coryne, API zym
[Ref.: #31595]	catalase	+	1.11.1.6
[Ref.: #120327]	catalase	+	1.11.1.6
[Ref.: #68379]	gelatinase	-		* from API Coryne
[Ref.: #68382]	leucine arylamidase	+	3.4.11.1	* from API zym
[Ref.: #68379]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API Coryne, API zym
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	+		* from API zym
[Ref.: #120327]	oxidase	-
[Ref.: #68379]	pyrazinamidase	-	3.5.1.B15	* from API Coryne
[Ref.: #68379]	pyrrolidonyl arylamidase	-	3.4.19.3	* from API Coryne
[Ref.: #68382]	trypsin	-	3.4.21.4	* from API zym
[Ref.: #31595]	urease	+	3.5.1.5
[Ref.: #120327]	urease	+	3.5.1.5
	Only first 10 entries are displayed. Click here to see all.

API® 50CH acid:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	ControlQ	Acid from glycerolGLY	Acid from erythritolERY	Acid from D-arabinoseDARA	Acid from L-arabinoseLARA	Acid from D-riboseRIB	Acid from D-xyloseDXYL	Acid from L-xyloseLXYL	Acid from D-adonitolADO	Acid from methyl beta-D-xylopyranosideMDX	Acid from D-galactoseGAL	Acid from D-glucoseGLU	Acid from D-fructoseFRU	Acid from D-mannoseMNE	Acid from L-sorboseSBE	Acid from L-rhamnoseRHA	Acid from dulcitolDUL	Acid from inositolINO	Acid from D-mannitolMAN	Acid from D-sorbitolSOR	Acid from methyl alpha-D-mannopyranosideMDM	Acid from methyl alpha-D-glucopyranosideMDG	Acid from N-acetyl-glucosamineNAG	Acid from amygdalinAMY	Acid from arbutinARB	Acid from esculinESC	Acid from salicinSAL	Acid from cellobioseCEL	Acid from maltoseMAL	Acid from lactoseLAC	Acid from melibioseMEL	Acid from sucroseSAC	Acid from trehaloseTRE	Acid from inulinINU	Acid from melezitoseMLZ	Acid from raffinoseRAF	Acid from starchAMD	Acid from glycogenGLYG	Acid from xylitolXLT	Acid from gentiobioseGEN	Acid from turanoseTUR	Acid from D-lyxoseLYX	Acid from D-tagatoseTAG	Acid from D-fucoseDFUC	Acid from L-fucoseLFUC	Acid from D-arabitolDARL	Acid from L-arabitolLARL	Acid from gluconateGNT	Acid from 2-Ketogluconate2KG	Acid from 5-Ketogluconate5KG
[Ref.: #120327]	11490	not determinedn.d.	+	-	-	+	+	+	-	-	-	+	+	+	+	-	-	-	-	+	-	+/-	+	+	+	+	+	+	+	+	+	+	+	+	-	-	-	-	-	-	+	+	-	+	-	-	-	-	+/-	-	-

API® Coryne:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Reduction of nitrateNIT	PyrazinamidasePYZ	Pyrrolidonyl arylamidasePYRA	Alkaline phosphatasePAL	beta-Glucuronidasebeta GUR	beta-Galactosidasebeta GAL	alpha-Glucosidasealpha GLU	N-Acetyl-beta-Glucosidasebeta NAG	Esculin hydrolysis/beta-GlucosidaseESC	Urease/urea hydrolysisURE	Gelatinase/gelatin hydrolysisGEL	Control fermentationControl	Fermentation of D-glucoseGLU	Fermentation of D-riboseRIB	Fermentation of D-xyloseXYL	Fermentation of D-mannitolMAN	Fermentation of maltoseMAL	Fermentation of lactoseLAC	Fermentation of sucroseSAC	Fermentation of glycogenGLYG	CatalaseCAT
[Ref.: #69369]	3862	+	-	-	+	-	-	+	-	+/-	+/-	-	not determinedn.d.	-	-	-	-	-	-	-	-	not determinedn.d.

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #69369]	6046	not determinedn.d.	+	+/-	+/-	+/-	+	+/-	+/-	-	-	+	+	-	+/-	-	+	+	-	+/-	-
[Ref.: #120327]	11702	-	+	+	+	-	+	-	-	-	-	+	+	-	-	-	+	+	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #12212]	Sample type/isolated from	natural cave
[Ref.: #12212]	Geographic location (country and/or sea, region)	Jeju Island
[Ref.: #12212]	Country	Republic of Korea
[Ref.: #12212]	Country ISO 3 Code	KOR
[Ref.: #12212]	Continent	Asia

[Ref.: #67770]	Sample type/isolated from	Soil inside a natural cave
[Ref.: #67770]	Geographic location (country and/or sea, region)	Jeju Island
[Ref.: #67770]	Country	Republic of Korea
[Ref.: #67770]	Country ISO 3 Code	KOR
[Ref.: #67770]	Continent	Asia

[Ref.: #120327]	Sample type/isolated from	Environment, Soil sample
[Ref.: #120327]	Geographic location (country and/or sea, region)	Jeju Island
[Ref.: #120327]	Country	Republic of Korea
[Ref.: #120327]	Country ISO 3 Code	KOR
[Ref.: #120327]	Continent	Asia
* marker position based on {}

Isolation sources categories

#Environmental

#Terrestrial

#Geologic

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence GQ376174 (>99% sequence identity) for Nocardia jejuensis subclade from Microbeatlas

Aquatic Samples	281
Soil Samples	520
Animal Samples	576
Plant Samples	54
Total Samples	1431

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #12212]	Biosafety level	1	Risk group (German classification) According to TRBA 466
[Ref.: #19894]	Biosafety level	1	Risk group (German classification)
[Ref.: #120327]	Biosafety level	2	Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #12212]	Nocardia jejuensis strain N3-2 16S ribosomal RNA gene, partial sequence	AY964666	1395	ENA	328049 tax ID
[Ref.: #20218]	Nocardia jejuensis 16S ribosomal RNA gene, partial sequence	GQ376174	1437	ENA	328049 tax ID	*

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Nocardia jejuensis NBRC 103114	GCA_001613145	contig	GenBank	1210088 tax ID
[Ref.: #66792]	Nocardia jejuensis NBRC 103114	1210088.4	wgs	PATRIC	1210088 tax ID	*
[Ref.: #66792]	Nocardia jejuensis NBRC 103114	2681812981	draft	JGI IMG/M	1210088 tax ID	*

[Ref.: #12212]	GC-content	69.6 mol%
[Ref.: #67770]	GC-content	69.6 mol%	high performance liquid chromatography (HPLC)
[Ref.: #67770]	GC-content	67.6 mol%	genome sequence analysis

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Nocardia jejuensis NBRC 103114 PATRIC: 1210088.4
[Ref.: #69481]	spore-forming	yes	100	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Nocardia jejuensis NBRC 103114 PATRIC: 1210088.4
[Ref.: #69480]	motile	no	93.129	yes
[Ref.: #69480]	gram-positive	yes	88.678	no
[Ref.: #69480]	anaerobic	no	99.523	yes
[Ref.: #69480]	aerobic	yes	92.051	yes
[Ref.: #69480]	halophile	no	94.231	no
[Ref.: #69480]	spore-forming	yes	87.825	yes
[Ref.: #69480]	thermophile	no	99.641	no
[Ref.: #69480]	glucose-util	yes	89.199	yes
[Ref.: #69480]	flagellated	no	97.89	yes
[Ref.: #69480]	glucose-ferment	no	90.316	yes

Availability in culture collections External links