Nocardia pneumoniae DSM 44730 is an aerobe, mesophilic, Gram-positive prokaryote that was isolated from human.
Gram-positive rod-shaped aerobe mesophilic genome sequence 16S sequence| @ref 20215 |
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| Domain Bacteria |
| Phylum Actinomycetota |
| Class Actinomycetes |
| Order Mycobacteriales |
| Family Nocardiaceae |
| Genus Nocardia |
| Species Nocardia pneumoniae |
| Full scientific name Nocardia pneumoniae Kageyama et al. 2004 |
| 31219 | Productionno |
| @ref: | 12048 |
| multimedia content: | DSM_44730.jpg |
| multimedia.multimedia content: | https://www.dsmz.de/microorganisms/photos/DSM_44730.jpg |
| caption: | Medium 535 28°C |
| intellectual property rights: | © Leibniz-Institut DSMZ |
| manual_annotation: | 1 |
| @ref | Name | Growth | Medium link | Composition | |
|---|---|---|---|---|---|
| 12048 | TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535) | Medium recipe at MediaDive | Name: TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Distilled water | ||
| 19698 | ISP 2 | Name: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes | |||
| 19698 | ISP 3 | Name: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms: | |||
| 19698 | ISP 4 | Name: ISP 4; DSM 547 Solution I: Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml. Solution II: CaCO3 2.0 g K2HPO4 (anhydrous) 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g (NH4)2SO4 2.0 g Distilled water 500.0 ml Trace salt solution (see below) 1.0 ml The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min. Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml | |||
| 19698 | ISP 5 | Name: ISP 5 (5323) Composition L-Asparagine 1.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l Salt solution (see preparation) 1.0 ml/l Agar 20.0 g/l Preparation: Salt solution 1.0 g FeSO4 x 7 H2O 1.0 g MnCl2 x 4 H2O 1.0 g ZNSO4 x 7 H2O in 100 ml water Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.2 Usage: Maintenance and taxonomy Organisms: All Actinomycetes | |||
| 19698 | ISP 6 | Name: ISP 6 (5318) Composition Peptone 15.0 g/l Proteose peptose 5.0 g/l Ferric ammonium citrate 0.5 g/l Sodium glycerophosphate 1.0 g/l Sodium thiosulfate 0.08 g/l Yeast extract 1.0 g/l Agar 15.0 g/l Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Production of melanoid pigments Organisms: All Actinomycetes | |||
| 19698 | ISP 7 | Name: ISP 7 (5322) Composition Glycerol 15.0 g/l L-Tyrosine 0.5 g/l L-Asparagine 1.0 g/l K2HPO4 0.5 g/l NaCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Trace element solution 5343 1.0 ml/l Agar 20.0 Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.3 Usage: Production of melanoid pigments Organisms: All Actinomycetes | |||
| 40047 | MEDIUM 72- for trypto casein soja agar | Distilled water make up to (1000.000 ml);Trypto casein soy agar (40.000 g) | |||
| 121894 | CIP Medium 72 | Medium recipe at CIP | |||
| 121894 | CIP Medium 112 | Medium recipe at CIP |
| 67770 | Observationquinones: MK-8(H4, ω-cycl) |
| @ref | Chebi-ID | Metabolite | Utilization activity | Kind of utilization tested | |
|---|---|---|---|---|---|
| 31219 | 30089 ChEBI | acetate | + | carbon source | |
| 19698 | 22599 ChEBI | arabinose | - | ||
| 19698 | 62968 ChEBI | cellulose | +/- | ||
| 31219 | 16947 ChEBI | citrate | + | carbon source | |
| 121894 | 16947 ChEBI | citrate | - | carbon source | |
| 31219 | 4853 ChEBI | esculin | + | hydrolysis | |
| 121894 | 4853 ChEBI | esculin | + | hydrolysis | |
| 19698 | 28757 ChEBI | fructose | + | ||
| 68379 | 5291 ChEBI | gelatin | + | hydrolysis | from API Coryne |
| 19698 | 17234 ChEBI | glucose | + | ||
| 31219 | 17234 ChEBI | glucose | + | carbon source | |
| 121894 | 606565 ChEBI | hippurate | + | hydrolysis | |
| 19698 | 29864 ChEBI | mannitol | - | ||
| 19698 | 17268 ChEBI | myo-inositol | - | ||
| 31219 | 17632 ChEBI | nitrate | + | reduction | |
| 121894 | 17632 ChEBI | nitrate | + | reduction | |
| 121894 | 17632 ChEBI | nitrate | - | respiration | |
| 121894 | 16301 ChEBI | nitrite | - | reduction | |
| 19698 | 16634 ChEBI | raffinose | - | ||
| 19698 | 26546 ChEBI | rhamnose | +/- | ||
| 19698 | 17992 ChEBI | sucrose | + | ||
| 68379 | 17992 ChEBI | sucrose | - | fermentation | from API Coryne |
| 68379 | 16199 ChEBI | urea | + | hydrolysis | from API Coryne |
| 19698 | 18222 ChEBI | xylose | - |
| @ref | Metabolite | Is sensitive | Is resistant | |
|---|---|---|---|---|
| 121894 | 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate) |
| @ref | Value | Activity | Ec | |
|---|---|---|---|---|
| 68382 | acid phosphatase | + | 3.1.3.2 | from API zym |
| 121894 | alcohol dehydrogenase | - | 1.1.1.1 | |
| 68382 | alkaline phosphatase | + | 3.1.3.1 | from API zym |
| 68379 | alkaline phosphatase | + | 3.1.3.1 | from API Coryne |
| 68382 | alpha-chymotrypsin | - | 3.4.21.1 | from API zym |
| 68382 | alpha-fucosidase | - | 3.2.1.51 | from API zym |
| 68382 | alpha-galactosidase | - | 3.2.1.22 | from API zym |
| 68382 | alpha-glucosidase | + | 3.2.1.20 | from API zym |
| 68379 | alpha-glucosidase | + | 3.2.1.20 | from API Coryne |
| 68382 | alpha-mannosidase | - | 3.2.1.24 | from API zym |
| 121894 | amylase | - | ||
| 31219 | arylsulfatase | + | 3.1.6.1 | |
| 121894 | beta-galactosidase | + | 3.2.1.23 | |
| 68379 | beta-galactosidase | - | 3.2.1.23 | from API Coryne |
| 68382 | beta-glucosidase | + | 3.2.1.21 | from API zym |
| 68382 | beta-glucuronidase | - | 3.2.1.31 | from API zym |
| 68379 | beta-glucuronidase | - | 3.2.1.31 | from API Coryne |
| 121894 | caseinase | - | 3.4.21.50 | |
| 121894 | catalase | + | 1.11.1.6 | |
| 121894 | DNase | - | ||
| 68382 | esterase (C 4) | + | from API zym | |
| 68382 | esterase lipase (C 8) | + | from API zym | |
| 121894 | gamma-glutamyltransferase | - | 2.3.2.2 | |
| 121894 | gelatinase | + | ||
| 68379 | gelatinase | + | from API Coryne | |
| 121894 | lecithinase | - | ||
| 68382 | leucine arylamidase | + | 3.4.11.1 | from API zym |
| 121894 | lipase | - | ||
| 68382 | lipase (C 14) | - | from API zym | |
| 121894 | lysine decarboxylase | - | 4.1.1.18 | |
| 68382 | N-acetyl-beta-glucosaminidase | - | 3.2.1.52 | from API zym |
| 68379 | N-acetyl-beta-glucosaminidase | - | 3.2.1.52 | from API Coryne |
| 68382 | naphthol-AS-BI-phosphohydrolase | + | from API zym | |
| 121894 | ornithine decarboxylase | - | 4.1.1.17 | |
| 121894 | oxidase | - | ||
| 121894 | phenylalanine ammonia-lyase | - | 4.3.1.24 | |
| 68379 | pyrazinamidase | - | 3.5.1.B15 | from API Coryne |
| 68379 | pyrrolidonyl arylamidase | - | 3.4.19.3 | from API Coryne |
| 68382 | trypsin | + | 3.4.21.4 | from API zym |
| 121894 | tryptophan deaminase | - | ||
| 121894 | tween esterase | - | ||
| 121894 | urease | - | 3.5.1.5 | |
| 68379 | urease | + | 3.5.1.5 | from API Coryne |
Global distribution of 16S sequence GQ853075 (>99% sequence identity) for Nocardia from Microbeatlas ![]()
| @ref | Description | Assembly level | INSDC accession | BV-BRC accession | IMG accession | NCBI tax ID | Score | |
|---|---|---|---|---|---|---|---|---|
| 67770 | ASM30875v1 assembly for Nocardia pneumoniae NBRC 100136 | contig | 1206737 | 46.81 |
| @ref | Description | Accession | Length | Database | NCBI tax ID | |
|---|---|---|---|---|---|---|
| 20218 | Nocardia pneumoniae strain DSM 44730 isolate VNS40 16S ribosomal RNA gene, partial sequence | AY903622 | 606 | 228601 | ||
| 20218 | Nocardia pneumoniae strain DSM 44730 16S ribosomal RNA gene, partial sequence | JF797313 | 1414 | 228601 | ||
| 20218 | Nocardia pneumoniae strain DSM 44730 16S ribosomal RNA gene, partial sequence | JN041465 | 462 | 228601 | ||
| 20218 | Nocardia pneumoniae strain W9813 16S ribosomal RNA gene, partial sequence | GQ853075 | 1441 | 228601 | ||
| 31219 | Nocardia pneumoniae gene for 16S rRNA, partial sequence | AB108780 | 1453 | 228601 |
| @ref | Trait | Model | Prediction | Confidence in % | In training data |
|---|---|---|---|---|---|
| 125439 | spore_formation | BacteriaNetⓘ | yes | 76.70 | no |
| 125439 | motility | BacteriaNetⓘ | no | 89.10 | no |
| 125439 | gram_stain | BacteriaNetⓘ | positive | 99.20 | no |
| 125439 | oxygen_tolerance | BacteriaNetⓘ | obligate aerobe | 99.30 | no |
| @ref | Trait | Model | Prediction | Confidence in % | In training data |
|---|---|---|---|---|---|
| 125438 | gram-positive | gram-positiveⓘ | yes | 90.05 | yes |
| 125438 | anaerobic | anaerobicⓘ | no | 96.90 | yes |
| 125438 | spore-forming | spore-formingⓘ | yes | 62.92 | no |
| 125438 | aerobic | aerobicⓘ | yes | 89.74 | yes |
| 125438 | thermophilic | thermophileⓘ | no | 96.00 | yes |
| 125438 | flagellated | motile2+ⓘ | no | 89.00 | no |
| Topic | Title | Authors | Journal | DOI | Year | |
|---|---|---|---|---|---|---|
| Discovery and Characterization of the Fully Decorated Nocardiosis-Associated Polyketide Natural Product. | Kishore S, Del Rio Flores A, Lynch SR, Yuet KP, Khosla C. | J Am Chem Soc | 10.1021/jacs.3c13670 | 2024 | ||
| Enzymology | Evaluation of the integrated database network system (IDNS) SmartGene software for analysis of 16S rRNA gene sequences for identification of Nocardia species. | Conville PS, Murray PR, Zelazny AM. | J Clin Microbiol | 10.1128/jcm.00681-10 | 2010 | |
| Pulmonary Nocardiosis in a Patient With COPD and Bronchiectasis. | Mira Palomino C, Babiano Nodal M, Raboso Moreno B. | Open Respir Arch | 10.1016/j.opresp.2025.100485 | 2025 | ||
| Pathogenic Nocardia amamiensis infection: A rare case report and literature review. | Zhang J, Zhu Y, Sun Y, Han X, Mao Y. | Heliyon | 10.1016/j.heliyon.2023.e17183 | 2023 | ||
| Clinical features and outcomes of persons with cystic fibrosis and nocardia isolation: a systematic review. | Terlizzi V, Ballerini T, Castaldo A, Dolce D, Campana S, Taccetti G, Chiappini E. | BMC Pulm Med | 10.1186/s12890-024-03217-0 | 2024 | ||
| Identification of Nocardia and non-tuberculous Mycobacterium species by MALDI-TOF MS using the VITEK MS coupled to IVD and RUO databases. | Rodriguez-Temporal D, Zvezdanova ME, Benedi P, Marin M, Blazquez-Sanchez M, Ruiz-Serrano MJ, Munoz P, Rodriguez-Sanchez B. | Microb Biotechnol | 10.1111/1751-7915.14146 | 2023 | ||
| Pathogenicity | Retrospective Analysis of Antimicrobial Susceptibility Profiles of Nocardia Species from a Tertiary Hospital and Reference Laboratory, 2011 to 2017. | Hamdi AM, Fida M, Deml SM, Abu Saleh OM, Wengenack NL. | Antimicrob Agents Chemother | 10.1128/aac.01868-19 | 2020 | |
| Phylogeny | Clinical significance, antimicrobial susceptibility and molecular identification of Nocardia species isolated from children with cystic fibrosis. | Betran A, Villuendas MC, Rezusta A, Pereira J, Revillo MJ, Rodriguez-Nava V. | Braz J Microbiol | 10.1016/j.bjm.2016.01.029 | 2016 | |
| Genetics | Respiratory Bacterial Microbiota and Individual Bacterial Variability in Lung Cancer and Bronchiectasis Patients. | Ekanayake A, Madegedara D, Chandrasekharan V, Magana-Arachchi D. | Indian J Microbiol | 10.1007/s12088-019-00850-w | 2020 | |
| Phylogeny | Detection and characterization of nocardia from patients diagnosed as tuberculosis in egypt. | Helal ZH, Khan MI, Ashour MS, Eissa SA. | Int J Biomed Sci | 2008 | ||
| Phylogeny | Evaluation of Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry for Identification of Mycobacterium species, Nocardia species, and Other Aerobic Actinomycetes. | Buckwalter SP, Olson SL, Connelly BJ, Lucas BC, Rodning AA, Walchak RC, Deml SM, Wohlfiel SL, Wengenack NL. | J Clin Microbiol | 10.1128/jcm.02128-15 | 2016 | |
| Nocardia arthritidis as a cause of disseminated nocardiosis in a patient with chronic lymphocytic leukemia. | Roberts AL, Davidson RM, Freifeld AG, Iwen PC. | IDCases | 10.1016/j.idcr.2016.09.015 | 2016 | ||
| Metabolism | Diversity and antimicrobial activities of actinobacteria isolated from tropical mangrove sediments in Malaysia. | Lee LH, Zainal N, Azman AS, Eng SK, Goh BH, Yin WF, Ab Mutalib NS, Chan KG. | ScientificWorldJournal | 10.1155/2014/698178 | 2014 | |
| Phylogeny | Nocardia amamiensis sp. nov., isolated from a sugar-cane field in Japan. | Yamamura H, Tamura T, Sakiyama Y, Harayama S. | Int J Syst Evol Microbiol | 10.1099/ijs.0.64829-0 | 2007 | |
| Phylogeny | Nocardia niwae sp. nov., isolated from human pulmonary sources. | Moser BD, Klenk HP, Schumann P, Potter G, Lasker BA, Steigerwalt AG, Hinrikson HP, Brown JM | Int J Syst Evol Microbiol | 10.1099/ijs.0.020370-0 | 2010 | |
| Phylogeny | Nocardia araoensis sp. nov. and Nocardia pneumoniae sp. nov., isolated from patients in Japan. | Kageyama A, Yazawa K, Mukai A, Kohara T, Nishimura K, Kroppenstedt RM, Mikami Y | Int J Syst Evol Microbiol | 10.1099/ijs.0.63074-0 | 2004 |
| #12048 | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 44730 |
| #19698 | Wink, J.: Compendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig . |
| #20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 ) |
| #20218 | Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 ) |
| #27545 | IJSEM 2025 2004 ( DOI 10.1099/ijs.0.63074-0 , PubMed 15545428 ) |
| #31219 | Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #27545 |
| #40047 | ; Curators of the CIP; |
| #67770 | Japan Collection of Microorganism (JCM) ; Curators of the JCM; |
| #68379 | Automatically annotated from API Coryne . |
| #68382 | Automatically annotated from API zym . |
| #69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta . |
| #121894 | Collection of Institut Pasteur ; Curators of the CIP; CIP 108644 |
| #125438 | Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 ) |
| #125439 | Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 . |
| #126262 | A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 ) |
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