Nocardia asiatica DSM 44668 is an aerobe, spore-forming, mesophilic prokaryote that builds an aerial mycelium and was isolated from human sputum.
spore-forming Gram-positive aerobe mesophilic genome sequence 16S sequence| @ref 20215 |
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| Domain Bacillati |
| Phylum Actinomycetota |
| Class Actinomycetes |
| Order Mycobacteriales |
| Family Nocardiaceae |
| Genus Nocardia |
| Species Nocardia asiatica |
| Full scientific name Nocardia asiatica Kageyama et al. 2004 |
| BacDive ID | Other strains from Nocardia asiatica (1) | Type strain |
|---|---|---|
| 10776 | N. asiatica DSM 44700, IFM 10205, JCM 11961 |
| 29922 | Productionyes |
| @ref: | 11864 |
| multimedia content: | DSM_44668.jpg |
| multimedia.multimedia content: | https://www.dsmz.de/microorganisms/photos/DSM_44668.jpg |
| caption: | Medium 65 28°C |
| intellectual property rights: | © Leibniz-Institut DSMZ |
| manual_annotation: | 1 |
| @ref | Name | Growth | Medium link | Composition | |
|---|---|---|---|---|---|
| 11864 | GPHF-MEDIUM (DSMZ Medium 553) | Medium recipe at MediaDive | Name: GPHF-MEDIUM (DSMZ Medium 553) Composition: Agar 20.0 g/l Glucose 10.0 g/l Beef extract 5.0 g/l Yeast extract 5.0 g/l Casein peptone 5.0 g/l CaCl2 x 2 H2O 0.74 g/l Distilled water | ||
| 11864 | GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) | Medium recipe at MediaDive | Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 18.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water | ||
| 20089 | ISP 2 | Name: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes | |||
| 20089 | ISP 3 | Name: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms: | |||
| 20089 | ISP 4 | Name: ISP 4; DSM 547 Solution I: Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml. Solution II: CaCO3 2.0 g K2HPO4 (anhydrous) 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g (NH4)2SO4 2.0 g Distilled water 500.0 ml Trace salt solution (see below) 1.0 ml The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min. Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml | |||
| 20089 | ISP 5 | Name: ISP 5 (5323) Composition L-Asparagine 1.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l Salt solution (see preparation) 1.0 ml/l Agar 20.0 g/l Preparation: Salt solution 1.0 g FeSO4 x 7 H2O 1.0 g MnCl2 x 4 H2O 1.0 g ZNSO4 x 7 H2O in 100 ml water Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.2 Usage: Maintenance and taxonomy Organisms: All Actinomycetes | |||
| 20089 | ISP 6 | Name: ISP 6 (5318) Composition Peptone 15.0 g/l Proteose peptose 5.0 g/l Ferric ammonium citrate 0.5 g/l Sodium glycerophosphate 1.0 g/l Sodium thiosulfate 0.08 g/l Yeast extract 1.0 g/l Agar 15.0 g/l Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Production of melanoid pigments Organisms: All Actinomycetes | |||
| 20089 | ISP 7 | Name: ISP 7 (5322) Composition Glycerol 15.0 g/l L-Tyrosine 0.5 g/l L-Asparagine 1.0 g/l K2HPO4 0.5 g/l NaCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Trace element solution 5343 1.0 ml/l Agar 20.0 Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.3 Usage: Production of melanoid pigments Organisms: All Actinomycetes | |||
| 32913 | MEDIUM 116 - for Streptomyces, Nocardia, Streptosporangium and Mycobacterium chlorophenolicum | Distilled water make up to (1000.000 ml);Agar (20.000 g);Glucose (4.000g);Yeast extract (4.000 g);Malt extract (10.000 g) | |||
| 116526 | CIP Medium 116 | Medium recipe at CIP |
| 29922 | Spore formationyes |
| 67770 | Observationquinones: MK-8(H4, ω-cycl) |
| @ref | Chebi-ID | Metabolite | Utilization activity | Kind of utilization tested | |
|---|---|---|---|---|---|
| 29922 | 30089 ChEBI | acetate | + | carbon source | |
| 20089 | 22599 ChEBI | arabinose | - | ||
| 20089 | 62968 ChEBI | cellulose | - | ||
| 29922 | 16947 ChEBI | citrate | + | carbon source | |
| 116526 | 16947 ChEBI | citrate | - | carbon source | |
| 68379 | 4853 ChEBI | esculin | - | hydrolysis | from API Coryne |
| 116526 | 4853 ChEBI | esculin | + | hydrolysis | |
| 20089 | 28757 ChEBI | fructose | - | ||
| 68379 | 5291 ChEBI | gelatin | - | hydrolysis | from API Coryne |
| 20089 | 17234 ChEBI | glucose | + | ||
| 29922 | 17234 ChEBI | glucose | + | carbon source | |
| 116526 | 606565 ChEBI | hippurate | - | hydrolysis | |
| 20089 | 29864 ChEBI | mannitol | - | ||
| 20089 | 17268 ChEBI | myo-inositol | +/- | ||
| 29922 | 17632 ChEBI | nitrate | + | reduction | |
| 116526 | 17632 ChEBI | nitrate | + | reduction | |
| 116526 | 17632 ChEBI | nitrate | - | respiration | |
| 116526 | 16301 ChEBI | nitrite | - | reduction | |
| 20089 | 16634 ChEBI | raffinose | - | ||
| 20089 | 26546 ChEBI | rhamnose | - | ||
| 29922 | 26546 ChEBI | rhamnose | + | carbon source | |
| 20089 | 17992 ChEBI | sucrose | - | ||
| 68379 | 17992 ChEBI | sucrose | - | fermentation | from API Coryne |
| 68379 | 16199 ChEBI | urea | - | hydrolysis | from API Coryne |
| 20089 | 18222 ChEBI | xylose | - |
| @ref | Metabolite | Is antibiotic | Is sensitive | Is resistant | |
|---|---|---|---|---|---|
| 116526 | 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate) |
| @ref | Value | Activity | Ec | |
|---|---|---|---|---|
| 68382 | acid phosphatase | + | 3.1.3.2 | from API zym |
| 116526 | alcohol dehydrogenase | - | 1.1.1.1 | |
| 68382 | alkaline phosphatase | + | 3.1.3.1 | from API zym |
| 68379 | alkaline phosphatase | + | 3.1.3.1 | from API Coryne |
| 68382 | alpha-chymotrypsin | - | 3.4.21.1 | from API zym |
| 68382 | alpha-fucosidase | - | 3.2.1.51 | from API zym |
| 68382 | alpha-galactosidase | - | 3.2.1.22 | from API zym |
| 68382 | alpha-glucosidase | + | 3.2.1.20 | from API zym |
| 68379 | alpha-glucosidase | + | 3.2.1.20 | from API Coryne |
| 68382 | alpha-mannosidase | - | 3.2.1.24 | from API zym |
| 116526 | amylase | - | ||
| 68382 | beta-galactosidase | - | 3.2.1.23 | from API zym |
| 116526 | beta-galactosidase | - | 3.2.1.23 | |
| 68379 | beta-galactosidase | - | 3.2.1.23 | from API Coryne |
| 68382 | beta-glucosidase | + | 3.2.1.21 | from API zym |
| 68379 | beta-glucosidase | - | 3.2.1.21 | from API Coryne |
| 68379 | beta-glucuronidase | - | 3.2.1.31 | from API Coryne |
| 68382 | beta-glucuronidase | - | 3.2.1.31 | from API zym |
| 116526 | caseinase | - | 3.4.21.50 | |
| 116526 | catalase | + | 1.11.1.6 | |
| 68382 | cystine arylamidase | - | 3.4.11.3 | from API zym |
| 116526 | DNase | - | ||
| 68382 | esterase (C 4) | + | from API zym | |
| 68382 | esterase lipase (C 8) | + | from API zym | |
| 116526 | gamma-glutamyltransferase | + | 2.3.2.2 | |
| 116526 | gelatinase | - | ||
| 68379 | gelatinase | - | from API Coryne | |
| 116526 | lecithinase | - | ||
| 68382 | leucine arylamidase | + | 3.4.11.1 | from API zym |
| 116526 | lipase | - | ||
| 68382 | lipase (C 14) | - | from API zym | |
| 116526 | lysine decarboxylase | - | 4.1.1.18 | |
| 68382 | N-acetyl-beta-glucosaminidase | - | 3.2.1.52 | from API zym |
| 68379 | N-acetyl-beta-glucosaminidase | - | 3.2.1.52 | from API Coryne |
| 116526 | ornithine decarboxylase | - | 4.1.1.17 | |
| 116526 | oxidase | - | ||
| 116526 | phenylalanine ammonia-lyase | - | 4.3.1.24 | |
| 116526 | protease | - | ||
| 68379 | pyrazinamidase | - | 3.5.1.B15 | from API Coryne |
| 68379 | pyrrolidonyl arylamidase | - | 3.4.19.3 | from API Coryne |
| 68382 | trypsin | - | 3.4.21.4 | from API zym |
| 116526 | tryptophan deaminase | - | ||
| 116526 | tween esterase | - | ||
| 116526 | urease | - | 3.5.1.5 | |
| 68379 | urease | - | 3.5.1.5 | from API Coryne |
| 68382 | valine arylamidase | - | from API zym |
Global distribution of 16S sequence GQ217495 (>99% sequence identity) for Nocardia from Microbeatlas ![]()
| @ref | Description | Assembly level | INSDC accession | BV-BRC accession | IMG accession | NCBI tax ID | |
|---|---|---|---|---|---|---|---|
| 67770 | ASM30841v1 assembly for Nocardia asiatica NBRC 100129 | contig | 1206721 |
| @ref | Description | Accession | Length | Database | NCBI tax ID | |
|---|---|---|---|---|---|---|
| 20218 | Nocardia asiatica strain DSM 44668 isolate VNS35 16S ribosomal RNA gene, partial sequence | AY903617 | 606 | 1206721 | ||
| 20218 | Nocardia asiatica strain DSM 44668 16S ribosomal RNA gene, partial sequence | DQ659897 | 1370 | 1206721 | ||
| 20218 | Nocardia asiatica culture-collection CDC | GQ217495 | 1441 | 209252 | ||
| 20218 | Nocardia asiatica NBRC 100129 strain DSM 44668 16S ribosomal RNA gene, partial sequence | JN041487 | 462 | 1206721 | ||
| 11864 | Nocardia asiatica gene for 16S rRNA, partial sequence, strain: IFM 0245 | AB092566 | 1403 | 1206721 | ||
| 124043 | Nocardia asiatica NBRC 100129 strain DSM 44668 16S ribosomal RNA gene, partial sequence. | MW563862 | 1348 | 1206721 |
| @ref | Trait | Model | Prediction | Confidence in % | In training data |
|---|---|---|---|---|---|
| 125439 | oxygen_tolerance | BacteriaNetⓘ | obligate aerobe | 96.00 | no |
| 125439 | gram_stain | BacteriaNetⓘ | positive | 98.90 | no |
| 125439 | motility | BacteriaNetⓘ | no | 88.30 | no |
| 125439 | spore_formation | BacteriaNetⓘ | yes | 69.10 | no |
| @ref | Trait | Model | Prediction | Confidence in % | In training data |
|---|---|---|---|---|---|
| 125438 | gram-positive | gram-positiveⓘ | yes | 91.52 | yes |
| 125438 | anaerobic | anaerobicⓘ | no | 96.32 | yes |
| 125438 | spore-forming | spore-formingⓘ | yes | 62.99 | no |
| 125438 | aerobic | aerobicⓘ | yes | 89.97 | yes |
| 125438 | thermophilic | thermophileⓘ | no | 95.00 | yes |
| 125438 | flagellated | motile2+ⓘ | no | 87.00 | yes |
| Topic | Title | Authors | Journal | DOI | Year | |
|---|---|---|---|---|---|---|
| Metabolism | Characterization and functional expression of a rubber degradation gene of a Nocardia degrader from a rubber-processing factory. | Linh DV, Huong NL, Tabata M, Imai S, Iijima S, Kasai D, Anh TK, Fukuda M. | J Biosci Bioeng | 10.1016/j.jbiosc.2016.11.012 | 2017 | |
| Metabolism | Identification of poly(cis-1,4-Isoprene) degradation intermediates during growth of moderately thermophilic actinomycetes on rubber and cloning of a functional lcp homologue from Nocardia farcinica strain E1. | Ibrahim EM, Arenskotter M, Luftmann H, Steinbuchel A. | Appl Environ Microbiol | 10.1128/aem.72.5.3375-3382.2006 | 2006 | |
| Phylogeny | Genomic Virulence Features of Two Novel Species Nocardia barduliensis sp. nov. and Nocardia gipuzkoensis sp. nov., Isolated from Patients with Chronic Pulmonary Diseases. | Nouioui I, Cortes-Albayay C, Neumann-Schaal M, Vicente D, Cilla G, Klenk HP, Marimon JM, Ercibengoa M | Microorganisms | 10.3390/microorganisms8101517 | 2020 | |
| Phylogeny | Nocardia niwae sp. nov., isolated from human pulmonary sources. | Moser BD, Klenk HP, Schumann P, Potter G, Lasker BA, Steigerwalt AG, Hinrikson HP, Brown JM | Int J Syst Evol Microbiol | 10.1099/ijs.0.020370-0 | 2010 | |
| Phylogeny | Nocardia asiatica sp. nov., isolated from patients with nocardiosis in Japan and clinical specimens from Thailand. | Kageyama A, Poonwan N, Yazawa K, Mikami Y, Nishimura K | Int J Syst Evol Microbiol | 10.1099/ijs.0.02676-0 | 2004 |
| #11864 | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 44668 |
| #20089 | Wink, J.: Compendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig . |
| #20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 ) |
| #20218 | Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 ) |
| #29922 | Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #26293 (see below) |
| #32913 | ; Curators of the CIP; |
| #58347 | Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 48826 |
| #67770 | Japan Collection of Microorganism (JCM) ; Curators of the JCM; |
| #68379 | Automatically annotated from API Coryne . |
| #68382 | Automatically annotated from API zym . |
| #69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta . |
| #116526 | Collection of Institut Pasteur ; Curators of the CIP; CIP 108374 |
| #124043 | Isabel Schober, Julia Koblitz: Data extracted from sequence databases, automatically matched based on designation and taxonomy . |
| #125438 | Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 ) |
| #125439 | Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 . |
| #126262 | A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 ) |
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