Nocardia neocaledoniensis SBHROA6 is an aerobe, Gram-positive, rod-shaped bacterium that builds an aerial mycelium and was isolated from soil.
Gram-positive rod-shaped aerobe genome sequence 16S sequence Bacteria| @ref 20215 |
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| Domain Bacteria |
| Phylum Actinomycetota |
| Class Actinomycetes |
| Order Mycobacteriales |
| Family Nocardiaceae |
| Genus Nocardia |
| Species Nocardia neocaledoniensis |
| Full scientific name Nocardia neocaledoniensis Saintpierre-Bonaccio et al. 2004 |
| @ref | Name | Growth | Medium link | Composition | |
|---|---|---|---|---|---|
| 12036 | GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) | Medium recipe at MediaDive | Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 18.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water | ||
| 19695 | ISP 2 | Name: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes | |||
| 19695 | ISP 3 | Name: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms: | |||
| 19695 | ISP 4 | Name: ISP 4; DSM 547 Solution I: Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml. Solution II: CaCO3 2.0 g K2HPO4 (anhydrous) 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g (NH4)2SO4 2.0 g Distilled water 500.0 ml Trace salt solution (see below) 1.0 ml The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min. Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml | |||
| 19695 | ISP 5 | Name: ISP 5 (5323) Composition L-Asparagine 1.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l Salt solution (see preparation) 1.0 ml/l Agar 20.0 g/l Preparation: Salt solution 1.0 g FeSO4 x 7 H2O 1.0 g MnCl2 x 4 H2O 1.0 g ZNSO4 x 7 H2O in 100 ml water Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.2 Usage: Maintenance and taxonomy Organisms: All Actinomycetes | |||
| 19695 | ISP 6 | Name: ISP 6 (5318) Composition Peptone 15.0 g/l Proteose peptose 5.0 g/l Ferric ammonium citrate 0.5 g/l Sodium glycerophosphate 1.0 g/l Sodium thiosulfate 0.08 g/l Yeast extract 1.0 g/l Agar 15.0 g/l Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Production of melanoid pigments Organisms: All Actinomycetes | |||
| 19695 | ISP 7 | Name: ISP 7 (5322) Composition Glycerol 15.0 g/l L-Tyrosine 0.5 g/l L-Asparagine 1.0 g/l K2HPO4 0.5 g/l NaCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Trace element solution 5343 1.0 ml/l Agar 20.0 Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.3 Usage: Production of melanoid pigments Organisms: All Actinomycetes | |||
| 36732 | MEDIUM 72- for trypto casein soja agar | Distilled water make up to (1000.000 ml);Trypto casein soy agar (40.000 g) | |||
| 121267 | CIP Medium 72 | Medium recipe at CIP |
| 67770 | Observationquinones: MK-8(H6, ω-cycl) |
| @ref | Chebi-ID | Metabolite | Utilization activity | Kind of utilization tested | |
|---|---|---|---|---|---|
| 19695 | 22599 ChEBI | arabinose | - | ||
| 19695 | 62968 ChEBI | cellulose | - | ||
| 121267 | 16947 ChEBI | citrate | - | carbon source | |
| 30048 | 4853 ChEBI | esculin | + | hydrolysis | |
| 68379 | 4853 ChEBI | esculin | + | hydrolysis | from API Coryne |
| 121267 | 4853 ChEBI | esculin | + | hydrolysis | |
| 19695 | 28757 ChEBI | fructose | + | ||
| 30048 | 28757 ChEBI | fructose | + | carbon source | |
| 68379 | 5291 ChEBI | gelatin | - | hydrolysis | from API Coryne |
| 19695 | 17234 ChEBI | glucose | + | ||
| 121267 | 606565 ChEBI | hippurate | - | hydrolysis | |
| 19695 | 29864 ChEBI | mannitol | + | ||
| 30048 | 29864 ChEBI | mannitol | + | carbon source | |
| 30048 | 37684 ChEBI | mannose | + | carbon source | |
| 19695 | 17268 ChEBI | myo-inositol | - | ||
| 121267 | 17632 ChEBI | nitrate | + | reduction | |
| 121267 | 17632 ChEBI | nitrate | - | respiration | |
| 121267 | 16301 ChEBI | nitrite | - | reduction | |
| 19695 | 16634 ChEBI | raffinose | - | ||
| 30048 | 16634 ChEBI | raffinose | + | carbon source | |
| 19695 | 26546 ChEBI | rhamnose | - | ||
| 19695 | 17992 ChEBI | sucrose | - | ||
| 68379 | 17992 ChEBI | sucrose | + | fermentation | from API Coryne |
| 30048 | 27082 ChEBI | trehalose | + | carbon source | |
| 30048 | 53426 ChEBI | tween 80 | + | carbon source | |
| 30048 | 16199 ChEBI | urea | + | carbon source | |
| 68379 | 16199 ChEBI | urea | + | hydrolysis | from API Coryne |
| 19695 | 18222 ChEBI | xylose | - |
| @ref | Metabolite | Is sensitive | Is resistant | |
|---|---|---|---|---|
| 121267 | 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate) |
| @ref | Value | Activity | Ec | |
|---|---|---|---|---|
| 68382 | acid phosphatase | + | 3.1.3.2 | from API zym |
| 121267 | alcohol dehydrogenase | - | 1.1.1.1 | |
| 68382 | alkaline phosphatase | + | 3.1.3.1 | from API zym |
| 68379 | alkaline phosphatase | + | 3.1.3.1 | from API Coryne |
| 68382 | alpha-chymotrypsin | - | 3.4.21.1 | from API zym |
| 68382 | alpha-fucosidase | - | 3.2.1.51 | from API zym |
| 68382 | alpha-galactosidase | - | 3.2.1.22 | from API zym |
| 68379 | alpha-glucosidase | + | 3.2.1.20 | from API Coryne |
| 68382 | alpha-glucosidase | + | 3.2.1.20 | from API zym |
| 68382 | alpha-mannosidase | - | 3.2.1.24 | from API zym |
| 121267 | amylase | - | ||
| 68382 | beta-galactosidase | + | 3.2.1.23 | from API zym |
| 121267 | beta-galactosidase | + | 3.2.1.23 | |
| 68379 | beta-galactosidase | - | 3.2.1.23 | from API Coryne |
| 68382 | beta-glucosidase | + | 3.2.1.21 | from API zym |
| 68379 | beta-glucosidase | + | 3.2.1.21 | from API Coryne |
| 68382 | beta-glucuronidase | - | 3.2.1.31 | from API zym |
| 68379 | beta-glucuronidase | - | 3.2.1.31 | from API Coryne |
| 121267 | caseinase | - | 3.4.21.50 | |
| 30048 | catalase | + | 1.11.1.6 | |
| 121267 | catalase | + | 1.11.1.6 | |
| 121267 | DNase | - | ||
| 68382 | esterase (C 4) | + | from API zym | |
| 68382 | esterase lipase (C 8) | + | from API zym | |
| 121267 | gamma-glutamyltransferase | + | 2.3.2.2 | |
| 121267 | gelatinase | - | ||
| 68379 | gelatinase | - | from API Coryne | |
| 121267 | lecithinase | - | ||
| 68382 | leucine arylamidase | + | 3.4.11.1 | from API zym |
| 121267 | lipase | - | ||
| 68382 | lipase (C 14) | - | from API zym | |
| 121267 | lysine decarboxylase | - | 4.1.1.18 | |
| 68382 | N-acetyl-beta-glucosaminidase | - | 3.2.1.52 | from API zym |
| 68379 | N-acetyl-beta-glucosaminidase | - | 3.2.1.52 | from API Coryne |
| 68382 | naphthol-AS-BI-phosphohydrolase | + | from API zym | |
| 121267 | ornithine decarboxylase | - | 4.1.1.17 | |
| 121267 | oxidase | - | ||
| 121267 | phenylalanine ammonia-lyase | - | 4.3.1.24 | |
| 68379 | pyrazinamidase | + | 3.5.1.B15 | from API Coryne |
| 68379 | pyrrolidonyl arylamidase | - | 3.4.19.3 | from API Coryne |
| 121267 | tryptophan deaminase | - | ||
| 121267 | tween esterase | - | ||
| 121267 | urease | + | 3.5.1.5 | |
| 68379 | urease | + | 3.5.1.5 | from API Coryne |
Global distribution of 16S sequence JF797311 (>99% sequence identity) for Nocardia from Microbeatlas ![]()
| @ref | Pathogenicity human | Pathogenicity animal | Biosafety level | Biosafety level comment | |
|---|---|---|---|---|---|
| 12036 | yes, in single cases | yes, in single cases | 1 | Risk group (German classification) According to TRBA 466 | |
| 19695 | 1 | Risk group (German classification) According to TRBA 466 | |||
| 121267 | 2 | Risk group (French classification) According to TRBA 466 |
| @ref | Description | Assembly level | INSDC accession | BV-BRC accession | IMG accession | NCBI tax ID | Score | |
|---|---|---|---|---|---|---|---|---|
| 67770 | ASM318213v1 assembly for Nocardia neocaledoniensis DSM 44717 | scaffold | 236511 | 71.51 | ||||
| 67770 | ASM799073v1 assembly for Nocardia neocaledoniensis NBRC 108232 | contig | 1210080 |
| @ref | Description | Accession | Length | Database | NCBI tax ID | |
|---|---|---|---|---|---|---|
| 20218 | Nocardia neocaledoniensis strain DSM 44717 isolate VNS32 16S ribosomal RNA gene, partial sequence | AY903614 | 606 | 236511 | ||
| 20218 | Nocardia neocaledoniensis strain W8372 16S ribosomal RNA gene, partial sequence | GQ853080 | 1441 | 236511 | ||
| 20218 | Nocardia neocaledoniensis strain DSM 44717 16S ribosomal RNA gene, partial sequence | JF797311 | 1395 | 236511 | ||
| 20218 | Nocardia neocaledoniensis strain DSM 44717 16S ribosomal RNA gene, partial sequence | JN041458 | 462 | 236511 | ||
| 12036 | Nocardia neocaledonica 16S ribosomal RNA gene, partial sequence | AY282603 | 1474 | 236511 |
| @ref | Trait | Model | Prediction | Confidence in % | In training data |
|---|---|---|---|---|---|
| 125439 | oxygen_tolerance | BacteriaNetⓘ | obligate aerobe | 97.10 | no |
| 125439 | gram_stain | BacteriaNetⓘ | positive | 99.00 | no |
| 125439 | motility | BacteriaNetⓘ | no | 84.80 | no |
| 125439 | spore_formation | BacteriaNetⓘ | yes | 64.30 | no |
| @ref | Trait | Model | Prediction | Confidence in % | In training data |
|---|---|---|---|---|---|
| 125438 | gram-positive | gram-positiveⓘ | yes | 90.59 | yes |
| 125438 | anaerobic | anaerobicⓘ | no | 98.50 | yes |
| 125438 | spore-forming | spore-formingⓘ | yes | 59.31 | no |
| 125438 | aerobic | aerobicⓘ | yes | 87.45 | yes |
| 125438 | thermophilic | thermophileⓘ | no | 95.90 | no |
| 125438 | flagellated | motile2+ⓘ | no | 89.00 | no |
| Topic | Title | Authors | Journal | DOI | Year | |
|---|---|---|---|---|---|---|
| Pathogenicity | Anti-Candida properties of urauchimycins from actinobacteria associated with trachymyrmex ants. | Mendes TD, Borges WS, Rodrigues A, Solomon SE, Vieira PC, Duarte MC, Pagnocca FC. | Biomed Res Int | 10.1155/2013/835081 | 2013 | |
| Enzymology | Purification and characterization of a new family 45 endoglucanase, STCE1, from Staphylotrichum coccosporum and its overproduction in Humicola insolens. | Koga J, Baba Y, Shimonaka A, Nishimura T, Hanamura S, Kono T. | Appl Environ Microbiol | 10.1128/aem.02747-07 | 2008 | |
| Phylogeny | Nocardia rhizosphaerihabitans sp. nov., a novel actinomycete isolated from a coastal soil. | Ding P, Bai JL, Wang TT, Sun Y, Cao CL, Jiang JH, Qin S | Int J Syst Evol Microbiol | 10.1099/ijsem.0.002481 | 2017 | |
| Phylogeny | Nocardia neocaledoniensis sp. nov., a novel actinomycete isolated from a New-Caledonian brown hypermagnesian ultramafic soil. | Saintpierre-Bonaccio D, Maldonado LA, Amir H, Pineau R, Goodfellow M | Int J Syst Evol Microbiol | 10.1099/ijs.0.02881-0 | 2004 |
| #12036 | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 44717 |
| #19695 | Wink, J.: Compendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig . |
| #20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 ) |
| #20218 | Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 ) |
| #26409 | IJSEM 599 2004 ( DOI 10.1099/ijs.0.02881-0 , PubMed 15023981 ) |
| #30048 | Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #26409 |
| #36732 | ; Curators of the CIP; |
| #67770 | Japan Collection of Microorganism (JCM) ; Curators of the JCM; |
| #68379 | Automatically annotated from API Coryne . |
| #68382 | Automatically annotated from API zym . |
| #69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta . |
| #121267 | Collection of Institut Pasteur ; Curators of the CIP; CIP 108429 |
| #125438 | Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 ) |
| #125439 | Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 . |
| #126262 | A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 ) |
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