Name: Bacillus atrophaeus DSM 7264
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 1077

Type strain:

Species: Bacillus atrophaeus

Culture col. no.: DSM 7264, ATCC 49337, NRRL NRS-213, CCUG 28524, JCM 9070, CIP 107159, IFO 15539, KCTC 3701, LMG 16797, NBRC 15539, NCIMB 12899

Strain history: NRRL NRS-213 <-- N. R. Smith NRS-213.

NCBI tax ID(s): 1220587 (strain), 1452 (species)

Other strains from Bacillus atrophaeus

B. atrophaeus 1221A, DSM 675, ATCC 9372, CIP ...
B. atrophaeus DSM 2277, ATCC 51189, CIP 103406, ...
B. atrophaeus DSM 5551, NRRL NRS-253, NRS 1936, ...
B. atrophaeus ES2-W11b1, DSM 30650
B. atrophaeus ES2-AirT4-1, DSM 30662
B. atrophaeus P4/15_1, DSM 30923
B. atrophaeus CIP 77.17
B. atrophaeus CIP 52.79
B. atrophaeus CCUG 31558
B. atrophaeus CCUG 44943
B. atrophaeus CCUG 44944
B. atrophaeus CCUG 59659 B
B. atrophaeus CCUG 61211

Section

Bacillus atrophaeus DSM 7264 is an aerobe, spore-forming, mesophilic human pathogen that forms circular colonies and has a dark brown pigmentation.

colony-forming
Gram-positive
motile
pigmented
rod-shaped
spore-forming
aerobe
human pathogen
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	07-12-2022 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacillota*
	Class	*Bacilli*
	Order	*Caryophanales*
	Family	*Bacillaceae*
	Genus	*Bacillus*
	Species	**Bacillus atrophaeus**
	Full Scientific Name (PNU)	Bacillus atrophaeus Nakamura 1989

Information on morphological and physiological properties Morphology

[Ref.: #23299]	Gram stain	positive
[Ref.: #23299]	Cell length	2.0-4.0 µm
[Ref.: #23299]	Cell width	0.5-1.0 µm
[Ref.: #23299]	Cell shape	rod-shaped
[Ref.: #23299]	Motility	yes

[Ref.: #23299]	Colony size	1-2 mm
[Ref.: #23299]	Colony color	opaque
[Ref.: #23299]	Colony shape	circular
[Ref.: #23299]	Incubation period	2 days

[Ref.: #23299]	Pigment production	yes
[Ref.: #23299]	Pigmentation color	dark brown

Information on culture and growth conditions Culture and growth conditions

[Ref.: #3083]

Culture medium

NUTRIENT AGAR (DSMZ Medium 1)

[Ref.: #3083]

Culture medium growth

[Ref.: #3083]

Culture medium link

Medium recipe provided by DSMZ

[Ref.: #3083]

Culture medium composition

expand / minimize

[Ref.: #36886]

Culture medium

MEDIUM 72- for trypto casein soja agar

[Ref.: #36886]

Culture medium growth

[Ref.: #36886]

Culture medium composition

expand / minimize

		Temperature
[Ref.: #3083]	growth	30 °C
[Ref.: #23299]	maximum	50-55 °C
[Ref.: #23299]	minimum	5-10 °C
[Ref.: #23299]	optimum	28-30 °C
[Ref.: #36886]	growth	30 °C
[Ref.: #67770]	growth	30 °C

[Ref.: #3083]	Temperature range	mesophilic
[Ref.: #23299]	Temperature range	thermophilic
[Ref.: #23299]	Temperature range	psychrophilic
[Ref.: #23299]	Temperature range	mesophilic
[Ref.: #36886]	Temperature range	mesophilic
[Ref.: #67770]	Temperature range	mesophilic

	pH	Kind of pH		pH	pH range
[Ref.: #23299]			growth	5.6-5.7

Information on physiology and metabolism Physiology and metabolism

[Ref.: #23299]

Oxygen tolerance

aerobe

[Ref.: #23299]	Spore description	ellipsoidal
[Ref.: #23299]	Type of spore	endospore
[Ref.: #23299]	Ability of spore formation	yes

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #23299]		NaCl		growth	7	%

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #23299]	arginine ChEBI	-	degradation
[Ref.: #23299]	casein	+	degradation
[Ref.: #23299]	casein	+	hydrolysis
[Ref.: #23299]	cellobiose ChEBI	+/-	builds acid from
[Ref.: #23299]	citrate ChEBI	+	assimilation
[Ref.: #23299]	D-fructose ChEBI	-	builds gas from
[Ref.: #23299]	D-fructose ChEBI	+	builds acid from
[Ref.: #23299]	D-galactose ChEBI	+/-	builds acid from
[Ref.: #23299]	D-glucose ChEBI	-	builds gas from
[Ref.: #23299]	D-glucose ChEBI	+	builds acid from
[Ref.: #23299]	D-mannose ChEBI	+/-	builds acid from
[Ref.: #23299]	D-ribose ChEBI	+/-	builds acid from
[Ref.: #23299]	D-xylose ChEBI	-	builds gas from
[Ref.: #23299]	D-xylose ChEBI	+	builds acid from
[Ref.: #23299]	egg yolk	-	degradation
[Ref.: #23299]	L-arabinose ChEBI	-	builds gas from
[Ref.: #23299]	L-arabinose ChEBI	+	builds acid from
[Ref.: #23299]	L-rhamnose ChEBI	+/-	builds acid from
[Ref.: #23299]	lactose ChEBI	-	fermentation
[Ref.: #23299]	lecithin ChEBI	-	degradation
[Ref.: #23299]	lysine ChEBI	-	degradation
[Ref.: #23299]	maltose ChEBI	+/-	builds acid from
[Ref.: #23299]	mannitol ChEBI	-	builds gas from
[Ref.: #23299]	mannitol ChEBI	+	builds acid from
[Ref.: #23299]	melibiose ChEBI	-	fermentation
[Ref.: #23299]	nitrate ChEBI	+	reduction
[Ref.: #23299]	ornithine ChEBI	-	degradation
[Ref.: #23299]	phenylalanine ChEBI	-	degradation
[Ref.: #23299]	propionate ChEBI	-	assimilation
[Ref.: #23299]	salicin ChEBI	-	builds gas from
[Ref.: #23299]	salicin ChEBI	+	builds acid from
[Ref.: #23299]	sorbitol ChEBI	+/-	builds acid from
[Ref.: #23299]	starch ChEBI	+	hydrolysis
[Ref.: #23299]	sucrose ChEBI	-	builds gas from
[Ref.: #23299]	sucrose ChEBI	+	builds acid from
[Ref.: #23299]	trehalose ChEBI	-	builds gas from
[Ref.: #23299]	trehalose ChEBI	+	builds acid from
[Ref.: #23299]	tween 80 ChEBI	-	degradation
[Ref.: #23299]	tyrosine ChEBI	-	degradation
[Ref.: #23299]	urea ChEBI	-	degradation
	Only first 10 entries are displayed. Click here to see all.

	Metabolite production	Metabolite
[Ref.: #23299]		acetoin ChEBI
[Ref.: #23299]		dihydroxyacetone ChEBI
[Ref.: #23299]		hydrogen sulfide ChEBI
[Ref.: #23299]		indole ChEBI

	Physiological tests	Metabolite	Voges-Proskauer-test
[Ref.: #23299]		acetoin ChEBI	+

	Enzymes	Enzyme	Enzyme activity	EC number
[Ref.: #23299]		catalase	+	1.11.1.6
[Ref.: #23299]		cytochrome oxidase	-	1.9.3.1

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #3083]	Sample type/isolated from	soil
[Ref.: #3083]	Geographic location (country and/or sea, region)	Colorado

[Ref.: #49270]	Sample type/isolated from	Soil
[Ref.: #49270]	Geographic location (country and/or sea, region)	Colorado
[Ref.: #49270]	Country	USA
[Ref.: #49270]	Country ISO 3 Code	USA
[Ref.: #49270]	Continent	North America

[Ref.: #67770]	Sample type/isolated from	Soil
[Ref.: #67770]	Geographic location (country and/or sea, region)	CO
[Ref.: #67770]	Country	USA
[Ref.: #67770]	Country ISO 3 Code	USA
[Ref.: #67770]	Continent	North America
* marker position based on {}

Isolation sources categories

#Environmental

#Terrestrial

#Soil

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence AB021181 (>99% sequence identity) for Bacillus from Microbeatlas

Aquatic Samples	40
Soil Samples	16
Animal Samples	61
Plant Samples	16
Total Samples	133

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #3083]	Pathogenicity (human)	yes, in single cases
[Ref.: #3083]	Biosafety level	1	Risk group (German classification) According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

Only first 5 entries are displayed. Click here to see all.

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #20218]	Bacillus atrophaeus clone EP-1_F 16S ribosomal RNA gene, partial sequence; 16S-23S internal transcribed spacer, complete sequence; and 23S ribosomal RNA gene, partial sequence	AF478080	415	ENA	1452 tax ID	*
[Ref.: #20218]	Bacillus atrophaeus strain BCRC 17123 16S ribosomal RNA gene, partial sequence	EF433409	1468	ENA	1452 tax ID	*
[Ref.: #20218]	Bacillus atrophaeus gene for 16S ribosomal RNA	AB021181	1515	ENA	1452 tax ID	*
[Ref.: #20218]	Bacillus atrophaeus gene for 16S rRNA, partial sequence, strain: NBRC 15539	AB363731	1475	ENA	1452 tax ID	*
[Ref.: #20218]	B.atrophaeus 16S ribosomal RNA	X60607	1427	ENA	1452 tax ID	*
[Ref.: #20218]	Bacillus atrophaeus strain NRRL NRS-213 16S ribosomal RNA gene, partial sequence	EU138516	1168	ENA	1452 tax ID	*

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Bacillus atrophaeus NBRC 15539	1220587.3	wgs	PATRIC	1220587 tax ID	*
[Ref.: #66792]	Bacillus atrophaeus NBRC 15539	2731957673	draft	JGI IMG/M	1220587 tax ID	*
[Ref.: #67770]	Bacillus atrophaeus NBRC 15539 NBRC 15539	GCA_001591925	contig	GenBank	1220587 tax ID
[Ref.: #67770]	Bacillus atrophaeus NRRL NRS 213	GCA_001584335	scaffold	GenBank	1452 tax ID
[Ref.: #66792]	Klebsiella grimontii RHBSTW-00853	GCA_013730235	complete	PATRIC	2058152 tax ID	*

[Ref.: #23299]

GC-content

41-43 mol%

Buoyant density centrifugation (BD)

Availability in culture collections External links

[Ref.: #3083]

Culture collection no.

DSM 7264, ATCC 49337, NRRL NRS-213, CCUG 28524, JCM 9070, CIP 107159, IFO 15539, KCTC 3701, LMG 16797, NBRC 15539, NCIMB 12899

[Ref.: #20218]

Associated Passport(s) in StrainInfo

160678, 33983, 291063

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	An algorithm for automated bacterial identification using matrix-assisted laser desorption/ionization mass spectrometry.	Jarman KH, Cebula ST, Saenz AJ, Petersen CE, Valentine NB, Kingsley MT, Wahl KL	Anal Chem	10.1021/ac990832j	2000	*
Biotechnology	Effect of microorganism characteristics on leak size critical to predicting package sterility.	Keller S, Marcy J, Blakistone B, Hackney C, Carter WH, Lacy G	J Food Prot	10.4315/0362-028x-66.9.1716	2003	*
Phylogeny	Identification of bacterial spores using statistical analysis of Fourier transform infrared photoacoustic spectroscopy data.	Thompson SE, Foster NS, Johnson TJ, Valentine NB, Amonette JE	Appl Spectrosc	10.1366/000370203322258832	2003	*
Phylogeny	Detection of molecular diversity in Bacillus atrophaeus by amplified fragment length polymorphism analysis.	Burke SA, Wright JD, Robinson MK, Bronk BV, Warren RL	Appl Environ Microbiol	10.1128/AEM.70.5.2786-2790.2004	2004	*
Pathogenicity	Role of pigmentation in protecting Bacillus sp. endospores against environmental UV radiation.	Moeller R, Horneck G, Facius R, Stackebrandt E	FEMS Microbiol Ecol	10.1016/j.femsec.2004.08.008	2005	*

References

#3083

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 7264

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#20218

Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )

#23299

L. K. NAKAMURA: Taxonomic Relationship of Black-Pigmented Bacillus subtilis Strains and a Proposal for Bacillus atrophaeus sp. nov.. IJSEM 39: 295 - 300 1989 ( DOI 10.1099/00207713-39-3-295 )

#36886 ; Curators of the CIP;
#49270 Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 28524

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

* These data were automatically processed and therefore are not curated

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