Nocardia nova 23095, 2338, R443(1), R443 | Type strain | DSM 44481, ATCC 33726, CCRC 13746, CIP 104777, IFM 0290, IFO 15556, JCM 6044, NBRC 15556, CCUG 45939, BCRC 13746, CECT 3056, CGMCC 4.1705, CGMCC 4.1879, IMSNU 21197, IMSNU 21201, KCTC 9959, VKM Ac-1971

Caption:

Medium 535 28°C

Intellectual property rights:

Information on culture and growth conditions Culture and growth conditions

Culture medium

TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535)

Culture medium growth

Medium recipe at MediaDive

Culture medium link

Culture medium composition

Culture medium

GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)

Culture medium growth

Medium recipe at MediaDive

Culture medium link

Culture medium composition

Culture medium

ISP 2

Culture medium growth

Culture medium composition

Culture medium

ISP 3

Culture medium growth

Culture medium composition

Culture medium

ISP 4

Culture medium growth

Culture medium composition

Culture medium

ISP 5

Culture medium growth

Culture medium composition

[Ref.: #35233]

Culture medium

MEDIUM 9 - Brain heart infusion BHI

[Ref.: #35233]

Culture medium growth

[Ref.: #35233]

Culture medium composition

Culture medium

CIP Medium 116

Culture medium growth

Culture medium link

Culture medium

CIP Medium 9

Culture medium growth

Culture medium link

Culture medium

CIP Medium 129

Culture medium growth

Culture medium link

Culture medium

CIP Medium 194

Culture medium growth

Culture medium link

Only first 5 entries are displayed. Click here to see all.

	Kind of temperature		Temperature
[Ref.: #11807]		growth	28 °C
[Ref.: #35233]		growth	30 °C
[Ref.: #56996]		growth	37 °C
[Ref.: #67770]		growth	28 °C
[Ref.: #121361]		growth	30-37 °C
[Ref.: #121361]	no	growth	10 °C
[Ref.: #121361]	no	growth	45 °C

Information on physiology and metabolism Physiology and metabolism

[Ref.: #56996]	Oxygen tolerance	aerobe
[Ref.: #121361]	Oxygen tolerance	obligate aerobe

	Salt	Tested relation		Salt conc.
[Ref.: #20062]	NaCl		maximum	2.5	%
[Ref.: #121361]	NaCl		growth	0-4	%
[Ref.: #121361]	NaCl	no	growth	6	%
[Ref.: #121361]	NaCl	no	growth	8	%
[Ref.: #121361]	NaCl	no	growth	10	%

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #68368]	arginine ChEBI	+	hydrolysis	* from API 20E
[Ref.: #68368]	citrate ChEBI	+	assimilation	* from API 20E
[Ref.: #121361]	esculin ChEBI	-	hydrolysis
[Ref.: #68368]	gelatin ChEBI	-	hydrolysis	* from API 20E
[Ref.: #121361]	hippurate ChEBI	+	hydrolysis
[Ref.: #68368]	lysine ChEBI	+	degradation	* from API 20E
[Ref.: #121361]	nitrate ChEBI	+	reduction
[Ref.: #121361]	nitrate ChEBI	-	respiration
[Ref.: #121361]	nitrite ChEBI	-	reduction
[Ref.: #68368]	ornithine ChEBI	+	degradation	* from API 20E
[Ref.: #68368]	tryptophan ChEBI	-	energy source	* from API 20E
[Ref.: #68368]	urea ChEBI	-	hydrolysis	* from API 20E
	Only first 10 entries are displayed. Click here to see all.

	Metabolite	Production
[Ref.: #121361]	indole ChEBI	no
[Ref.: #67770]	nocardimicin G	yes
[Ref.: #67770]	nocardimicin H	yes
[Ref.: #67770]	nocardimicin I	yes
[Ref.: #68368]	acetoin ChEBI	no	* from API 20E
[Ref.: #68368]	hydrogen sulfide ChEBI	no	* from API 20E
[Ref.: #68368]	indole ChEBI	no	* from API 20E

	Metabolite	Voges-Proskauer-test	Indole test
[Ref.: #121361]	acetoin ChEBI	-
[Ref.: #68368]	acetoin ChEBI	-		* from API 20E
[Ref.: #68368]	indole ChEBI		-	* from API 20E

	Enzyme	Enzyme activity	EC number
[Ref.: #121361]	alcohol dehydrogenase	-	1.1.1.1
[Ref.: #68382]	alpha-chymotrypsin	-	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	-	3.2.1.22	* from API zym
[Ref.: #68382]	alpha-glucosidase	+	3.2.1.20	* from API zym
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #121361]	amylase	-
[Ref.: #68368]	arginine dihydrolase	+	3.5.3.6	* from API 20E
[Ref.: #121361]	beta-galactosidase	+	3.2.1.23
[Ref.: #68368]	beta-galactosidase	-	3.2.1.23	* from API 20E
[Ref.: #68382]	beta-glucosidase	+	3.2.1.21	* from API zym
[Ref.: #68382]	beta-glucuronidase	-	3.2.1.31	* from API zym
[Ref.: #121361]	caseinase	-	3.4.21.50
[Ref.: #121361]	catalase	+	1.11.1.6
[Ref.: #121361]	DNase	-
[Ref.: #68382]	esterase lipase (C 8)	+		* from API zym
[Ref.: #121361]	gelatinase	-
[Ref.: #68368]	gelatinase	-		* from API 20E
[Ref.: #121361]	lecithinase	-
[Ref.: #68382]	leucine arylamidase	+	3.4.11.1	* from API zym
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #121361]	lysine decarboxylase	-	4.1.1.18
[Ref.: #68368]	lysine decarboxylase	+	4.1.1.18	* from API 20E
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API zym
[Ref.: #121361]	ornithine decarboxylase	-	4.1.1.17
[Ref.: #68368]	ornithine decarboxylase	+	4.1.1.17	* from API 20E
[Ref.: #121361]	oxidase	-
[Ref.: #68368]	tryptophan deaminase	-	4.1.99.1	* from API 20E
[Ref.: #121361]	tween esterase	-
[Ref.: #121361]	urease	+	3.5.1.5
[Ref.: #68368]	urease	-	3.5.1.5	* from API 20E
[Ref.: #68382]	valine arylamidase	+		* from API zym
	Only first 10 entries are displayed. Click here to see all.

API® 20E:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	beta-GalactosidaseONPG	Arginine dihydrolaseADH (Arg)	Lysine decarboxylaseLDC (Lys)	Ornithine decarboxylaseODC	Citrate utilizationCIT	H2S productionH2S	Urease/urea hydrolysisURE	Tryptophan deaminaseTDA (Trp)	Indole productionIND	Acetoin production (Voges Proskauer test)VP	Gelatinase/gelatin hydrolysisGEL	Fermentation/oxidation of D-glucoseGLU	Fermentation/oxidation of D-mannitolMAN	Fermentation/oxidation of inositolINO	Fermentation/oxidation of sorbitolSor	Fermentation/oxidation of L-rhamnoseRHA	Fermentation/oxidation of sucroseSAC	Fermentation/oxidation of melibioseMEL	Fermentation/oxidation of amygdalinAMY	Fermentation/oxidation of L-arabinoseARA	Cytochrome oxidaseOX	Nitrite productionNO2	Reduction to N2N2	MotilityMOB	Growth on MacConkey mediumMAC	Oxidative catabolisation of D-glucoseOF-O	Fermentation of D-glucoseOF-F
[Ref.: #20062]	626	-	+	+	+	+	-	-	-	-	-	-	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.

API® Biotype100:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	ControlQ	D-glucose as carbon sourceGLU	D-fructose as carbon sourceFRU	D-galactose as carbon sourceGAL	D-trehalose as carbon sourceTRE	D-mannose as carbon sourceMNE	L-sorbose as carbon sourceSBE	D-melibiose as carbon sourceMEL	sucrose as carbon sourceSAC	D-raffinose as carbon sourceRAF	maltotriose as carbon sourceMTE	maltose as carbon sourceMAL	lactose as carbon sourceLAC	lactulose as carbon sourceLTE	1-O-methyl-beta-galactopyranoside as carbon sourceMbGa	1-O-methyl-alpha-galactopyranoside as carbon sourceMaGa	D-cellobiose as carbon sourceCEL	gentiobiose as carbon sourceGEN	1-O-methyl-beta-D-glucopyranoside as carbon sourceMbGu	esculin as carbon sourceESC	D-ribose as carbon sourceRIB	L-arabinose as carbon sourceARA	D-xylose as carbon sourceXYL	palatinose as carbon sourcePLE	L-rhamnose as carbon sourceRHA	L-fucose as carbon sourceFUC	D-melezitose as carbon sourceMLZ	D-arabitol as carbon sourceDARL	L-arabitol as carbon sourceLARL	xylitol as carbon sourceXLT	dulcitol as carbon sourceDUL	D-tagatose as carbon sourceTAG	glycerol as carbon sourceGLY	myo-inositol as carbon sourceINO	D-mannitol as carbon sourceMAN	maltitol as carbon sourceMTL	D-turanose as carbon sourceTUR	D-sorbitol as carbon sourceSOR	adonitol as carbon sourceADO	hydroxyquinoline-beta-glucuronide as carbon sourceHBG	D-lyxose as carbon sourceLYX	i-erythritol as carbon sourceERY	1-O-methyl-alpha-D-glucoside as carbon sourceMDG	3-O-methyl-D-glucose as carbon source3MDG	D-saccharate as carbon sourceSAT	mucate as carbon sourceMUC	L-tartrate as carbon sourceLTAT	D-tartrate as carbon sourceDTAT	meso-tartrate as carbon sourceMTAT	D-malate as carbon sourceDMLT	L-malate as carbon sourceLMLT	cis-aconitate as carbon sourceCATE	trans-aconitate as carbon sourceTATE	tricarballylate as carbon sourceTTE	citrate as carbon sourceCIT	D-glucuronate as carbon sourceGRT	D-galacturonate as carbon sourceGAT	2-ketogluconate as carbon source2KG	5-ketogluconate as carbon source5KG	tryptophan as carbon sourceTRY	N-acetyl-D-glucosamine as carbon sourceNAG	D-gluconate as carbon sourceGNT	phenylacetate as carbon sourcePAC	protocatechuate as carbon sourcePAT	4-hydroxybenzoate as carbon sourcepOBE	quinate as carbon sourceQAT	gentisate as carbon sourceGTE	3-hydroxybenzoate as carbon sourcemOBE	benzoate as carbon sourceBAT	3-phenylpropionate as carbon sourcePPAT	m-coumarate as carbon sourceCMT	trigonelline as carbon sourceTGE	betaine as carbon sourceBET	putrescine as carbon sourcePCE	4-aminobutyrate as carbon sourceABT	histamine as carbon sourceHIN	DL-lactate as carbon sourceLAT	caprate as carbon sourceCAP	caprylate as carbon sourceCYT	L-histidine as carbon sourceHIS	succinate as carbon sourceSUC	fumarate as carbon sourceFUM	glutarate as carbon sourceGRE	DL-glycerate as carbon sourceGYT	5-aminovalerate as carbon sourceAVT	ethanolamine as carbon sourceETN	tryptamine as carbon sourceTTN	D-glucosamine as carbon sourceGLN	itaconate as carbon sourceITA	3-hydroxybutyrate as carbon source3OBU	L-aspartate as carbon sourceAPT	L-glutamate as carbon sourceGTT	L-proline as carbon sourcePRO	D-alanine as carbon sourceDALA	L-alanine as carbon sourceLALA	L-serine as carbon sourceSER	malonate as carbon sourceMNT	propionate as carbon sourcePROP	L-tyrosine as carbon sourceTYR	2-ketoglutarate as carbon source2KT
[Ref.: #121361]	816	not determinedn.d.	+	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	+	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	+	-	-	+	+	+	-	-	-	-	-	-	-	+	-	+	-	-	-	-	-	+	-	+

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #20062]	844	not determinedn.d.	-	-	+	-	+	+	-	-	-	-	-	-	-	-	+	+	-	-	-
[Ref.: #121361]	8191	-	+	+	+	-	+	+	+	+	-	+	+	-	+	-	+	+	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #69479]

Global distribution of 16S sequence X80593 (>99% sequence identity) for Nocardia nova from Microbeatlas

Aquatic Samples	11
Soil Samples	25
Animal Samples	6
Plant Samples	5
Total Samples	47

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #11807]	Pathogenicity (human)	yes
[Ref.: #11807]	Pathogenicity (animal)	yes
[Ref.: #11807]	Biosafety level	2	Risk group (German classification) According to TRBA 466
[Ref.: #20062]	Biosafety level	2	German classification
[Ref.: #121361]	Biosafety level	2	Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #11807]	Nocardia nova 16S rRNA gene, strain ATCC 33726T	X80593	1395	GenBank ENA	37330 tax ID
[Ref.: #11807]	N.nova gene for 16S ribosomal RNA	Z36930	1472	GenBank ENA	37330 tax ID
[Ref.: #20218]	Nocardia nova strain ATCC 33726T 16S ribosomal RNA gene, partial sequence	AY191250	1383	GenBank ENA	37330 tax ID	*
[Ref.: #20218]	Nocardia nova strain ATCC 33726 16S ribosomal RNA gene, partial sequence	DQ659911	1401	GenBank ENA	37330 tax ID	*
[Ref.: #20218]	Nocardia nova strain ATCC 33726 clone 1 16S ribosomal RNA gene, partial sequence; 16S-23S ribosomal RNA intergenic spacer, complete sequence; and 23S ribosomal RNA gene, partial sequence	GU236409	626	GenBank ENA	37330 tax ID	*
[Ref.: #20218]	Nocardia nova strain ATCC 33726 clone 2 16S ribosomal RNA gene, partial sequence; 16S-23S ribosomal RNA intergenic spacer, complete sequence; and 23S ribosomal RNA gene, partial sequence	GU236410	626	GenBank ENA	37330 tax ID	*
[Ref.: #20218]	Nocardia nova strain ATCC 33726 clone 3 16S ribosomal RNA gene, partial sequence; 16S-23S ribosomal RNA intergenic spacer, complete sequence; and 23S ribosomal RNA gene, partial sequence	GU236411	626	GenBank ENA	37330 tax ID	*
[Ref.: #20218]	Nocardia nova strain ATCC 33726 clone 4 16S ribosomal RNA gene, partial sequence; 16S-23S ribosomal RNA intergenic spacer, complete sequence; and 23S ribosomal RNA gene, partial sequence	GU236412	626	GenBank ENA	37330 tax ID	*
[Ref.: #20218]	Nocardia nova strain ATCC 33726 clone 5 16S ribosomal RNA gene, partial sequence; 16S-23S ribosomal RNA intergenic spacer, complete sequence; and 23S ribosomal RNA gene, partial sequence	GU236413	632	GenBank ENA	37330 tax ID	*
[Ref.: #20218]	Nocardia nova strain ATCC 33726 clone 6 16S ribosomal RNA gene, partial sequence; 16S-23S ribosomal RNA intergenic spacer, complete sequence; and 23S ribosomal RNA gene, partial sequence	GU236414	626	GenBank ENA	37330 tax ID	*
[Ref.: #20218]	Nocardia nova clone A9 16S ribosomal RNA gene, partial sequence	AY262320	500	GenBank ENA	37330 tax ID	*
[Ref.: #20218]	Nocardia nova strain JCM 6044 16S ribosomal RNA gene, complete sequence	AF430028	1492	GenBank ENA	37330 tax ID	*
[Ref.: #20218]	Nocardia nova strain JCM 6044 note copy 1 16S-23S ribosomal RNA intergenic spacer, complete sequence	AF536455	322	GenBank ENA	37330 tax ID	*
[Ref.: #20218]	Nocardia nova strain JCM 6044 note copy 2 16S-23S ribosomal RNA intergenic spacer, complete sequence	AF536456	322	GenBank ENA	37330 tax ID	*
[Ref.: #20218]	Nocardia nova strain JCM 6044 note copy 3 16S-23S ribosomal RNA intergenic spacer, complete sequence	AF536457	328	GenBank ENA	37330 tax ID	*
[Ref.: #20218]	Nocardia nova strain ATCC 33726 16S ribosomal RNA gene, partial sequence	GQ376190	1439	GenBank ENA	37330 tax ID	*
[Ref.: #20218]	Nocardia nova strain DSM 44481 16S ribosomal RNA gene, partial sequence	JN041650	462	GenBank ENA	37330 tax ID	*

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Nocardia nova NBRC 15556	GCA_001613005	contig	GenBank ENA	1210081 tax ID
[Ref.: #66792]	Nocardia nova NBRC 15556	1210081.4	wgs	PATRIC	1210081 tax ID	*
[Ref.: #66792]	Nocardia nova NBRC 15556	2731957739	draft	JGI IMG/M	1210081 tax ID	*

[Ref.: #11807]	GC-content	67.3 mol%	high performance liquid chromatography (HPLC)
[Ref.: #67770]	GC-content	67.9 mol%	genome sequence analysis

Data predicted using genome information as a basis Genome-based predictions

	Trait	Model	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Nocardia nova NBRC 15556 PATRIC: 1210081.4
[Ref.: #69481]	spore-forming	spore-formingⓘ	yes	83	no

	Trait	Model	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Nocardia nova NBRC 15556 PATRIC: 1210081.4
[Ref.: #69480]	gram-positive	gram-positiveⓘ	yes	90.66	no
[Ref.: #69480]	anaerobic	anaerobicⓘ	no	92.206	no
[Ref.: #69480]	spore-forming	spore-formingⓘ	yes	63.187	no
[Ref.: #69480]	aerobic	aerobicⓘ	yes	88.954	yes
[Ref.: #69480]	thermophilic	thermophileⓘ	no	96.4	yes
[Ref.: #69480]	flagellated	motile2+ⓘ	no	89.5	no

Availability in culture collections External links