Gordonia cholesterolivorans Chol-3 is an obligate aerobe, Gram-positive, rod-shaped bacterium that was isolated from sewage sludge from sewage treatment plant.
Gram-positive rod-shaped obligate aerobe genome sequence 16S sequence Bacteria| @ref 20215 |
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Last LPSN update
2026-02-09 11:20:18 |
| Domain Bacteria |
| Phylum Actinomycetota |
| Class Actinomycetes |
| Order Mycobacteriales |
| Family Gordoniaceae |
| Genus Gordonia |
| Species Gordonia cholesterolivorans |
| Full scientific name Gordonia cholesterolivorans Drzyzga et al. 2009 |
| @ref | Name | Growth | Medium link | Composition | |
|---|---|---|---|---|---|
| 15808 | BHI MEDIUM (DSMZ Medium 215) | Medium recipe at MediaDive  | Name: BHI MEDIUM (DSMZ Medium 215) Composition: Brain heart infusion 37.0 g/l Distilled water | ||
| 15808 | GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) | Medium recipe at MediaDive | Name: GYM STREPTOMYCES MEDIUM (DSMZ Medium 65) Composition: Agar 18.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water | ||
| 15808 | TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535) | Medium recipe at MediaDive | Name: TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Distilled water | ||
| 20185 | ISP 2 | Name: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes | |||
| 20185 | ISP 3 | Name: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms: | |||
| 20185 | ISP 4 | Name: ISP 4; DSM 547 Solution I: Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml. Solution II: CaCO3 2.0 g K2HPO4 (anhydrous) 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g (NH4)2SO4 2.0 g Distilled water 500.0 ml Trace salt solution (see below) 1.0 ml The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min. Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml | |||
| 20185 | ISP 5 | Name: ISP 5 (5323) Composition L-Asparagine 1.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l Salt solution (see preparation) 1.0 ml/l Agar 20.0 g/l Preparation: Salt solution 1.0 g FeSO4 x 7 H2O 1.0 g MnCl2 x 4 H2O 1.0 g ZNSO4 x 7 H2O in 100 ml water Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.2 Usage: Maintenance and taxonomy Organisms: All Actinomycetes | |||
| 20185 | ISP 6 | Name: ISP 6 (5318) Composition Peptone 15.0 g/l Proteose peptose 5.0 g/l Ferric ammonium citrate 0.5 g/l Sodium glycerophosphate 1.0 g/l Sodium thiosulfate 0.08 g/l Yeast extract 1.0 g/l Agar 15.0 g/l Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Production of melanoid pigments Organisms: All Actinomycetes | |||
| 20185 | ISP 7 | Name: ISP 7 (5322) Composition Glycerol 15.0 g/l L-Tyrosine 0.5 g/l L-Asparagine 1.0 g/l K2HPO4 0.5 g/l NaCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Trace element solution 5343 1.0 ml/l Agar 20.0 Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.3 Usage: Production of melanoid pigments Organisms: All Actinomycetes | |||
| 37917 | MEDIUM 72- for trypto casein soja agar | Distilled water make up to (1000.000 ml);Trypto casein soy agar (40.000 g) | |||
| 118253 | CIP Medium 72 | Medium recipe at CIP | |||
| 118253 | CIP Medium 368 | Medium recipe at CIP |
| 118253 | Oxygen toleranceobligate aerobe |
| @ref | Chebi-ID | Metabolite | Utilization activity | Kind of utilization tested | |
|---|---|---|---|---|---|
| 68379 | 17634 ChEBI | D-glucose | - | fermentation | from API Coryne |
| 68379 | 16899 ChEBI | D-mannitol | - | fermentation | from API Coryne |
| 68379 | 16988 ChEBI | D-ribose | - | fermentation | from API Coryne |
| 68379 | 65327 ChEBI | D-xylose | - | fermentation | from API Coryne |
| 68379 | 4853 ChEBI | esculin | - | hydrolysis | from API Coryne |
| 68379 | 5291 ChEBI | gelatin | - | hydrolysis | from API Coryne |
| 68379 | 28087 ChEBI | glycogen | - | fermentation | from API Coryne |
| 68379 | 17716 ChEBI | lactose | - | fermentation | from API Coryne |
| 68379 | 17306 ChEBI | maltose | - | fermentation | from API Coryne |
| 68379 | 17632 ChEBI | nitrate | - | reduction | from API Coryne |
| 118253 | 17632 ChEBI | nitrate | + | reduction | |
| 118253 | 16301 ChEBI | nitrite | - | reduction | |
| 68379 | 17992 ChEBI | sucrose | + | fermentation | from API Coryne |
| 68379 | 16199 ChEBI | urea | - | hydrolysis | from API Coryne |
| @ref | Value | Activity | Ec | |
|---|---|---|---|---|
| 68382 | acid phosphatase | + | 3.1.3.2 | from API zym |
| 68382 | alkaline phosphatase | + | 3.1.3.1 | from API zym |
| 68379 | alkaline phosphatase | + | 3.1.3.1 | from API Coryne |
| 68382 | alpha-chymotrypsin | - | 3.4.21.1 | from API zym |
| 68382 | alpha-fucosidase | - | 3.2.1.51 | from API zym |
| 68382 | alpha-galactosidase | - | 3.2.1.22 | from API zym |
| 68382 | alpha-glucosidase | + | 3.2.1.20 | from API zym |
| 68379 | alpha-glucosidase | + | 3.2.1.20 | from API Coryne |
| 68382 | alpha-mannosidase | - | 3.2.1.24 | from API zym |
| 68382 | beta-galactosidase | - | 3.2.1.23 | from API zym |
| 68379 | beta-galactosidase | - | 3.2.1.23 | from API Coryne |
| 68382 | beta-glucosidase | + | 3.2.1.21 | from API zym |
| 68379 | beta-glucosidase | - | 3.2.1.21 | from API Coryne |
| 68382 | beta-glucuronidase | - | 3.2.1.31 | from API zym |
| 68379 | beta-glucuronidase | - | 3.2.1.31 | from API Coryne |
| 118253 | catalase | + | 1.11.1.6 | |
| 68382 | esterase (C 4) | + | from API zym | |
| 68382 | esterase lipase (C 8) | + | from API zym | |
| 68379 | gelatinase | - | from API Coryne | |
| 68382 | leucine arylamidase | + | 3.4.11.1 | from API zym |
| 68382 | lipase (C 14) | + | from API zym | |
| 68382 | N-acetyl-beta-glucosaminidase | - | 3.2.1.52 | from API zym |
| 68379 | N-acetyl-beta-glucosaminidase | - | 3.2.1.52 | from API Coryne |
| 68382 | naphthol-AS-BI-phosphohydrolase | + | from API zym | |
| 118253 | oxidase | - | ||
| 68379 | pyrazinamidase | - | 3.5.1.B15 | from API Coryne |
| 68379 | pyrrolidonyl arylamidase | + | 3.4.19.3 | from API Coryne |
| 68382 | trypsin | - | 3.4.21.4 | from API zym |
| 118253 | urease | - | 3.5.1.5 | |
| 68379 | urease | - | 3.5.1.5 | from API Coryne |
| 68382 | valine arylamidase | + | from API zym |
| Cat1 | Cat2 | Cat3 | |
|---|---|---|---|
| #Engineered | #Waste | #Sewage sludge | |
| #Engineered | #Waste | #Water treatment plant |
| @ref | Sample type | Geographic location | Country | Country ISO 3 Code | Continent | Isolation date | |
|---|---|---|---|---|---|---|---|
| 15808 | sewage sludge from sewage treatment plant | Ciudad Real | Spain | ESP | Europe | ||
| 67770 | Sewage sludge from a wastewater treatment plant | Ciudad Real | Spain | ESP | Europe | ||
| 118253 | Environment, Sewage sludge sample | Ciudad Real, Madrid | Spain | ESP | Europe | 2006 |
Global distribution of 16S sequence EU244645 (>99% sequence identity) for Gordonia from Microbeatlas 
 Aquatic Samples |
620 |
 Soil Samples |
85 |
 Animal Samples |
263 |
 Plant Samples |
40 |
| Total Samples | 1008 |
| @ref | Description | Assembly level | INSDC accession | NCBI tax ID | Score | |
|---|---|---|---|---|---|---|
| 124043 | ASM3953197v1 assembly for Gordonia cholesterolivorans JCM 16227 | scaffold | GCA_039531975   | 559625 | 61.76 | |
| @ref | Description | Accession | Length | Database | NCBI tax ID | |
|---|---|---|---|---|---|---|
| 15808 | Gordonia cholesterolivorans strain Chol-3 16S ribosomal RNA gene, partial sequence | EU244645 | 1445 |  | 559625 |
| Topic | Title | Authors | Journal | DOI | Year | |
|---|---|---|---|---|---|---|
| Pathogenicity | Microbial biofilm formation and degradation of octocrylene, a UV absorber found in sunscreen. | Suleiman M, Schroder C, Kuhn M, Simon A, Stahl A, Frerichs H, Antranikian G. | Commun Biol | 10.1038/s42003-019-0679-9 | 2019 | |
| Mechanistic and phylogenetic insights into actinobacteria-mediated oestrogen biodegradation in urban estuarine sediments. | Hsiao TH, Chen YL, Meng M, Chuang MR, Horinouchi M, Hayashi T, Wang PH, Chiang YR. | Microb Biotechnol | 10.1111/1751-7915.13798 | 2021 | | |
| Metabolism | Estrogen Degraders and Estrogen Degradation Pathway Identified in an Activated Sludge. | Chen YL, Fu HY, Lee TH, Shih CJ, Huang L, Wang YS, Ismail W, Chiang YR. | Appl Environ Microbiol | 10.1128/aem.00001-18 | 2018 | |
| Metabolism | Cholesterol degradation by Gordonia cholesterolivorans. | Drzyzga O, Fernandez de las Heras L, Morales V, Navarro Llorens JM, Perera J. | Appl Environ Microbiol | 10.1128/aem.05149-11 | 2011 | |
| Phylogeny | Gordonia cholesterolivorans sp. nov., a cholesterol-degrading actinomycete isolated from sewage sludge. | Drzyzga O, Navarro Llorens JM, Fernandez de Las Heras L, Garcia Fernandez E, Perera J | Int J Syst Evol Microbiol | 10.1099/ijs.0.005777-0 | 2009 | |
How to citeIf you want to cite this particular strain cite the following doi:
https://doi.org/10.13145/bacdive10619.20251217.10
When using BacDive for research please cite the following paper
BacDive in 2025: the core database for prokaryotic strain data
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