Strain identifier

BacDive ID: 97

Type strain: Yes

Species: Acholeplasma laidlawii

Strain Designation: PG 8, PG8

Strain history: CIP <- 1975, ATCC <- E.A. Freundt: strain PG8 <- Nat. Inst. Med. Res. A <- P.P. Laidlaw and W.J. Elford

NCBI tax ID(s): 2148 (species)

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General

@ref: 16683

BacDive-ID: 97

DSM-Number: 23060

keywords: genome sequence, 16S sequence, Bacteria, mesophilic, animal pathogen

description: Acholeplasma laidlawii PG 8 is a mesophilic animal pathogen that was isolated from sewage.

NCBI tax id

  • NCBI tax id: 2148
  • Matching level: species

strain history

@refhistory
16683<- CIP <- ATCC <- Natl. Inst. Med. Res., London, United Kingdom; PG 8 <- P. P. Laidlaw and W. J. Elford
120550CIP <- 1975, ATCC <- E.A. Freundt: strain PG8 <- Nat. Inst. Med. Res. A <- P.P. Laidlaw and W.J. Elford

doi: 10.13145/bacdive97.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/mycoplasmatota
  • domain: Bacteria
  • phylum: Mycoplasmatota
  • class: Mollicutes
  • order: Acholeplasmatales
  • family: Acholeplasmataceae
  • genus: Acholeplasma
  • species: Acholeplasma laidlawii
  • full scientific name: Acholeplasma laidlawii (Sabin 1941) Edward and Freundt 1970 (Approved Lists 1980)

  • synonyms

    • @ref: 20215
    • synonym: Sapromyces laidlawi

@ref: 16683

domain: Bacteria

phylum: Tenericutes

class: Mollicutes

order: Acholeplasmatales

family: Acholeplasmataceae

genus: Acholeplasma

species: Acholeplasma laidlawii

full scientific name: Acholeplasma laidlawii (Sabin 1941) Edward and Freundt 1970

strain designation: PG 8, PG8

type strain: yes

Morphology

cell morphology

@refmotilityconfidencegram stain
69480no93.866
6948099.719positive
120550negative

colony morphology

  • @ref: 16683
  • incubation period: 1-2 days

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
16683SP4-Z MEDIUM (DSMZ Medium 1076b)yeshttps://mediadive.dsmz.de/medium/1076bName: SP4-Z MEDIUM (DSMZ Medium 1076b) Composition: Agar 10.0 g/l Tryptone 10.0 g/l Bacto peptone 5.0 g/l PPLO broth 3.5 g/l Urea 2.0 g/l Glucose 1.0 g/l None 1.0 g/l Yeast extract 0.4 g/l DNA 0.2 g/l CMRL 1066 Fetal bovine serum Swine serum Distilled water
32774MEDIUM 88 - for Mycoplasma and AcholeplasmayesDistilled water make up to (670.000 ml);PPLO broth (17.000 g);Sterile supplement - M00273 (210.000 ml);Yeast extract solution 25 % - M00263 (100.000 ml);0.1 % Phenol red - M00387 (20.000 ml)
120550CIP Medium 88yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=88
120550CIP Medium 749yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=749

culture temp

@refgrowthtypetemperaturerange
16683positivegrowth37mesophilic
32774positivegrowth37mesophilic

Physiology and metabolism

spore formation

@refspore formationconfidence
69481no100
69480no99.999

Isolation, sampling and environmental information

isolation

@refsample type
16683sewage
120550Environment, Sewage

isolation source categories

  • Cat1: #Engineered
  • Cat2: #Waste
  • Cat3: #Wastewater

taxonmaps

  • @ref: 69479
  • File name: preview.99_34358.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_117;96_702;97_820;98_951;99_34358&stattab=map
  • Last taxonomy: Acholeplasma laidlawii subclade
  • 16S sequence: U14905
  • Sequence Identity:
  • Total samples: 22
  • aquatic counts: 5
  • animal counts: 16
  • plant counts: 1

Safety information

risk assessment

@refpathogenicity animalbiosafety levelbiosafety level comment
16683yes2Risk group (German classification)
1205501Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Acholeplasma laidlawii 16S-23S ribosomal RNA intergenic spacer, complete sequence; and 23S ribosomal RNA gene, partial sequenceAF294996664ena2148
20218Acholeplasma laidlawii strain ATCC 23206 16S ribosomal RNA gene, partial sequence; 16S-23S ribosomal RNA intergenic spacer, complete sequence; and 23S ribosomal RNA gene, partial sequenceAY740436769ena2148
20218Acholeplasma laidlawii strain ATCC 23206 16S ribosomal RNA gene, partial sequence; 16S-23S ribosomal RNA intergenic spacer, complete sequence; and 23S ribosomal RNA gene, partial sequenceAY740437978ena2148
20218Acholeplasma laidlawii PG8 ATCC 23206 16S ribosomal RNA gene, partial sequenceU149051476ena2148

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Acholeplasma laidlawii DSM 23060GCA_003385765scaffoldncbi2148
66792Acholeplasma laidlawii NCTC10116GCA_900476025completencbi2148
66792Acholeplasma laidlawii strain DSM 230602148.14wgspatric2148
66792Acholeplasma laidlawii strain NCTC101162148.13completepatric2148
66792Acholeplasma laidlawii DSM 230602757320367draftimg2148
66792Acholeplasma laidlawii NCTC 101162826166747completeimg2148

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno100no
flagellatedno98.499no
gram-positiveyes54.128no
anaerobicno77.492no
aerobicno93.647no
halophileyes64.489no
spore-formingno95.355no
thermophileno98.644yes
glucose-utilyes88.121no
motileno95.395no
glucose-fermentyes59.513no

External links

@ref: 16683

culture collection no.: DSM 23060, ATCC 23206, CIP 75.27, IFO (now NBRC) 14400, NCTC 10116, CCUG 32754

straininfo link

  • @ref: 69780
  • straininfo: 37937

literature

topicPubmed-IDtitleauthorsjournalyearmeshtopic2DOI
Enzymology848216[Characterization of Acholeplasma Strains by Horizontal Polyacrylamide Flat Gel Electrophoresis (author's transl)].Boden K, Kirchhoff HZentralbl Bakteriol Orig A1977Acholeplasma/analysis/*classification, Animals, Bacterial Proteins/analysis, Electrophoresis, Polyacrylamide Gel/methods, Horses/microbiologyPhylogeny
Enzymology2326188Human ureaplasmas show diverse genome sizes by pulsed-field electrophoresis.Robertson JA, Pyle LE, Stemke GW, Finch LRNucleic Acids Res1990DNA, Bacterial/genetics/*isolation & purification, Electrophoresis, Agar Gel, *Genes, Bacterial, Humans, Molecular Weight, Mycoplasma/genetics, Restriction Mapping, Serotyping, Ureaplasma/*genetics/isolation & purificationPhylogeny10.1093/nar/18.6.1451
7373281Colony morphology, ultrastructure and morphogenesis in Mycoplasma hominis, Acholeplasma laidlawii and Ureaplasma urealyticum.Meloni GA, Bertoloni G, Busolo F, Conventi LJ Gen Microbiol1980Acholeplasma laidlawii/*ultrastructure, Clone Cells, Microscopy, Electron, Microscopy, Interference, Morphogenesis, Mycoplasma/*ultrastructure, Ureaplasma/*ultrastructure10.1099/00221287-116-2-435
Metabolism7655655[The phenotypic traits of Mollicutes as their possible phylogenetic markers].Skrypal' IH, Onyshchenko AM, Malynovs'ka LP, Tokovenko IP, Havrylko LOMikrobiol Z1995Fructose-Bisphosphatase/metabolism, Genetic Markers, Lectins/metabolism, N-Acetylneuraminic Acid, Phenotype, *Phylogeny, Sialic Acids/metabolism, Tenericutes/*genetics/metabolismPhylogeny
Phylogeny7663744Possible monosaccharide composition of glycopolymers located on the external side of membranes of cells of some mollicutes.Skripal' IG, Onishchenko AM, Gavrilko LOMikrobiol Z1995Cell Membrane/chemistry, Glycoproteins/analysis, Lectins, Monosaccharides/*analysis, Polysaccharides/analysis, Polysaccharides, Bacterial/*analysis, Species Specificity, Tenericutes/*chemistry
Enzymology20014143Sensitive and selective detection of mycoplasma in cell culture samples using cantilever sensors.Xu S, Sharma H, Mutharasan RBiotechnol Bioeng2010Acholeplasma laidlawii/*isolation & purification, Bacteriological Techniques/*methods, Biosensing Techniques/*methods, Calibration, Cell Culture Techniques/*methods, Cells, Cultured, Enzyme-Linked Immunosorbent Assay, Escherichia coli, Linear Models, Sensitivity and SpecificityPhylogeny10.1002/bit.22637

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
16683Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 23060)https://www.dsmz.de/collection/catalogue/details/culture/DSM-23060
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
32774Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/10764
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
69780Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID37937.1StrainInfo: A central database for resolving microbial strain identifiers
120550Curators of the CIPCollection of Institut Pasteur (CIP 75.27)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%2075.27