Strain identifier

BacDive ID: 899

Type strain: No

Species: Bacillus sp.

Strain Designation: Pac 2

Strain history: CIP <- 1992, F. Pichinoty, CNRS, Marseille, France: strain Pac 2

NCBI tax ID(s): 1409 (species)

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General

@ref: 3101

BacDive-ID: 899

DSM-Number: 7297

keywords: Bacteria, obligate aerobe, spore-forming, mesophilic, Gram-positive, motile, rod-shaped

description: Bacillus sp. Pac 2 is an obligate aerobe, spore-forming, mesophilic bacterium that was isolated from soil.

NCBI tax id

  • NCBI tax id: 1409
  • Matching level: species

strain history

@refhistory
3101<- F. Pichinoty
355621992, F. Pichinoty, CNRS, Marseille, France: strain Pac 2
116023CIP <- 1992, F. Pichinoty, CNRS, Marseille, France: strain Pac 2

doi: 10.13145/bacdive899.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/bacillota
  • domain: Bacteria
  • phylum: Bacillota
  • class: Bacilli
  • order: Caryophanales
  • family: Bacillaceae
  • genus: Bacillus
  • species: Bacillus sp.
  • full scientific name: Bacillus Cohn 1872 (Approved Lists 1980)
  • synonyms

    @refsynonym
    20215Quasibacillus
    20215Pseudobacillus
    20215Maribacillus

@ref: 3101

domain: Bacteria

phylum: Firmicutes

class: Bacilli

order: Bacillales

family: Bacillaceae

genus: Bacillus

species: Bacillus sp.

full scientific name: Bacillus sp.

strain designation: Pac 2

type strain: no

Morphology

cell morphology

  • @ref: 116023
  • gram stain: positive
  • cell shape: rod-shaped
  • motility: yes

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
3101NUTRIENT AGAR (DSMZ Medium 1)yeshttps://mediadive.dsmz.de/medium/1Name: NUTRIENT AGAR (DSMZ Medium 1) Composition: Agar 15.0 g/l Peptone 5.0 g/l Meat extract 3.0 g/l Distilled water
35562MEDIUM 3 - Columbia agaryesColumbia agar (39.000 g);distilled water (1000.000 ml)
116023CIP Medium 3yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=3

culture temp

@refgrowthtypetemperaturerange
3101positivegrowth30mesophilic
35562positivegrowth30mesophilic
116023positivegrowth30-45
116023nogrowth10psychrophilic
116023nogrowth55thermophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 116023
  • oxygen tolerance: obligate aerobe

spore formation

  • @ref: 116023
  • spore formation: yes

halophily

@refsaltgrowthtested relationconcentration
116023NaClpositivegrowth0-2 %
116023NaClnogrowth4 %
116023NaClnogrowth6 %
116023NaClnogrowth8 %
116023NaClnogrowth10 %

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
11602317632nitrate+reduction
11602316301nitrite-reduction
11602317632nitrate+respiration

metabolite production

  • @ref: 116023
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

metabolite tests

  • @ref: 116023
  • Chebi-ID: 15688
  • metabolite: acetoin
  • voges-proskauer-test: +

enzymes

@refvalueactivityec
116023oxidase-
116023alcohol dehydrogenase-1.1.1.1
116023gelatinase-
116023amylase-
116023caseinase-3.4.21.50
116023catalase+1.11.1.6
116023lecithinase+
116023lysine decarboxylase-4.1.1.18
116023ornithine decarboxylase-4.1.1.17
116023urease+3.5.1.5

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentisolation date
3101soilMarseilleFranceFRAEurope
116023Environment, SoilMarseilleFranceFRAEurope1980

isolation source categories

  • Cat1: #Environmental
  • Cat2: #Terrestrial
  • Cat3: #Soil

Safety information

risk assessment

  • @ref: 116023
  • biosafety level: 1
  • biosafety level comment: Risk group (French classification)

Sequence information

GC content

  • @ref: 3101
  • GC-content: 46.4
  • method: Buoyant density centrifugation (BD)

External links

@ref: 3101

culture collection no.: DSM 7297, CIP 103557, ATCC 51157

straininfo link

  • @ref: 70572
  • straininfo: 42834

Reference

@idauthorscataloguedoi/urltitle
3101Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 7297)https://www.dsmz.de/collection/catalogue/details/culture/DSM-7297
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
35562Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/15368
70572Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID42834.1StrainInfo: A central database for resolving microbial strain identifiers
116023Curators of the CIPCollection of Institut Pasteur (CIP 103557)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20103557