Strain identifier

BacDive ID: 8627

Type strain: Yes

Species: Mycoplasma leachii

Strain Designation: N29, PG50

Strain history: <- NCTC {2008} <- PHLS <- R. H. Leach <- G. S. Cottew <- G.C. Simmons and L.A.Y. Johnston

NCBI tax ID(s): 2105 (species)

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General

@ref: 15475

BacDive-ID: 8627

DSM-Number: 21131

keywords: genome sequence, 16S sequence, Bacteria, aerobe, mesophilic, animal pathogen

description: Mycoplasma leachii N29 is an aerobe, mesophilic animal pathogen that was isolated from calf, arthritic joint.

NCBI tax id

  • NCBI tax id: 2105
  • Matching level: species

strain history

  • @ref: 15475
  • history: <- NCTC {2008} <- PHLS <- R. H. Leach <- G. S. Cottew <- G.C. Simmons and L.A.Y. Johnston

doi: 10.13145/bacdive8627.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/mycoplasmatota
  • domain: Bacteria
  • phylum: Mycoplasmatota
  • class: Mollicutes
  • order: Mycoplasmatales
  • family: Mycoplasmataceae
  • genus: Mycoplasma
  • species: Mycoplasma leachii
  • full scientific name: Mycoplasma leachii Manso-Silván et al. 2009

@ref: 15475

domain: Bacteria

phylum: Tenericutes

class: Mollicutes

order: Mycoplasmatales

family: Mycoplasmataceae

genus: Mycoplasma

species: Mycoplasma leachii

full scientific name: Mycoplasma leachii Manso-Silván et al. 2009

strain designation: N29, PG50

type strain: yes

Morphology

cell morphology

  • @ref: 29074
  • motility: no

pigmentation

  • @ref: 29074
  • production: no

Culture and growth conditions

culture medium

  • @ref: 15475
  • name: SP4-Z MEDIUM (DSMZ Medium 1076b)
  • growth: yes
  • link: https://mediadive.dsmz.de/medium/1076b
  • composition: Name: SP4-Z MEDIUM (DSMZ Medium 1076b) Composition: Agar 10.0 g/l Tryptone 10.0 g/l Bacto peptone 5.0 g/l PPLO broth 3.5 g/l Urea 2.0 g/l Glucose 1.0 g/l None 1.0 g/l Yeast extract 0.4 g/l DNA 0.2 g/l CMRL 1066 Fetal bovine serum Swine serum Distilled water

culture temp

@refgrowthtypetemperaturerange
15475positivegrowth37mesophilic
29074positivegrowth28-37mesophilic
29074positiveoptimum37mesophilic

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
15475aerobe
29074facultative anaerobe

metabolite utilization

  • @ref: 29074
  • Chebi-ID: 17234
  • metabolite: glucose
  • utilization activity: +
  • kind of utilization tested: carbon source

Isolation, sampling and environmental information

isolation

  • @ref: 15475
  • sample type: calf, arthritic joint
  • country: Australia
  • origin.country: AUS
  • continent: Australia and Oceania

isolation source categories

Cat1Cat2Cat3
#Infection#Inflammation
#Host#Mammals#Bovinae (Cow, Cattle)
#Host Body-Site#Limb#Joint

taxonmaps

  • @ref: 69479
  • File name: preview.99_653.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_109;96_405;97_458;98_527;99_653&stattab=map
  • Last taxonomy: Mycoplasma
  • 16S sequence: U26054
  • Sequence Identity:
  • Total samples: 4646
  • soil counts: 14
  • aquatic counts: 107
  • animal counts: 4511
  • plant counts: 14

Safety information

risk assessment

  • @ref: 15475
  • pathogenicity animal: yes
  • biosafety level: 2
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 15475
  • description: Mycoplasma sp. PG50 (bovine group 7) 16S ribosomal RNA (rrnB) gene
  • accession: U26054
  • length: 1524
  • database: ena
  • NCBI tax ID: 880447

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Mycoplasma leachii PG50GCA_000183365completencbi880447
66792Mycoplasma leachii PG50880447.3completepatric880447
66792Mycoplasma leachii PG50649633074completeimg880447

GC content

  • @ref: 29074
  • GC-content: 25.4

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
motileno93.445yes
flagellatedno97.96yes
gram-positiveyes72.619no
anaerobicno96.263yes
aerobicno96.239no
halophileno50no
spore-formingno98.437no
thermophileno95.048yes
glucose-utilyes91.964yes
glucose-fermentyes82.475no

External links

@ref: 15475

culture collection no.: DSM 21131, NCTC 10133

straininfo link

  • @ref: 78008
  • straininfo: 114266

literature

  • topic: Phylogeny
  • Pubmed-ID: 19502315
  • title: Mycoplasma leachii sp. nov. as a new species designation for Mycoplasma sp. bovine group 7 of Leach, and reclassification of Mycoplasma mycoides subsp. mycoides LC as a serovar of Mycoplasma mycoides subsp. capri.
  • authors: Manso-Silvan L, Vilei EM, Sachse K, Djordjevic SP, Thiaucourt F, Frey J
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijs.0.005546-0
  • year: 2009
  • mesh: Animals, Arthritis, Infectious/microbiology/*veterinary, Australia, Cattle, Cattle Diseases/*microbiology, Genotype, Goat Diseases/*microbiology, Goats, Mycoplasma/*classification/genetics/pathogenicity, Mycoplasma Infections/microbiology/veterinary, Mycoplasma mycoides/*classification/genetics/pathogenicity, Phylogeny, Pleuropneumonia, Contagious/*microbiology, Serotyping, Species Specificity, Turkey
  • topic2: Genetics

Reference

@idauthorscataloguedoi/urltitleID_cross_referencepubmed
15475Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 21131)https://www.dsmz.de/collection/catalogue/details/culture/DSM-21131
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
29074Barberan A, Caceres Velazquez H, Jones S, Fierer N.10.1128/mSphere.00237-17Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information2550428776041
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
78008Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID114266.1StrainInfo: A central database for resolving microbial strain identifiers