Strain identifier

BacDive ID: 8605

Type strain: Yes

Species: Mycoplasmoides genitalium

Strain Designation: G37

Strain history: <- J. K. Davis {2007} <- J. G. Tully; G37

NCBI tax ID(s): 2097 (species)

For citation purpose refer to the digital object identifier (doi) of the current version.
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General

@ref: 8310

BacDive-ID: 8605

DSM-Number: 19775

keywords: mesophilic, Bacteria, 16S sequence

description: Mycoplasmoides genitalium G37 is a mesophilic bacterium that was isolated from urethra of male with non-gonococcal urethritis.

NCBI tax id

  • NCBI tax id: 2097
  • Matching level: species

strain history: <- J. K. Davis {2007} <- J. G. Tully; G37

doi: 10.13145/bacdive8605.20220920.7

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/mycoplasmatota
  • domain: Bacteria
  • phylum: Mycoplasmatota
  • class: Mollicutes
  • order: Mycoplasmoidales
  • family: Mycoplasmoidaceae
  • genus: Mycoplasmoides
  • species: Mycoplasmoides genitalium
  • full scientific name: Mycoplasmoides genitalium (Tully et al. 1983) Gupta et al. 2018
  • synonyms

    • @ref: 20215
    • synonym: Mycoplasma genitalium

@ref: 8310

domain: Bacteria

phylum: Tenericutes

class: Mollicutes

order: Mycoplasmatales

family: Mycoplasmoidaceae

genus: Mycoplasmoides

species: Mycoplasmoides genitalium

full scientific name: Mycoplasmoides genitalium (Tully et al. 1983) Gupta et al. 2018

strain designation: G37

type strain: yes

Morphology

colony morphology

  • @ref: 8310
  • incubation period: 1-2 days

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
8310SP4-Z MEDIUM (DSMZ Medium 1076b)yeshttps://bacmedia.dsmz.de/medium/1076bName: SP4-Z MEDIUM (DSMZ Medium 1076b) Composition: Agar 10.0 g/l Tryptone 10.0 g/l Bacto peptone 5.0 g/l PPLO broth 3.5 g/l Urea 2.0 g/l Glucose 1.0 g/l Arginine HCl 1.0 g/l Yeast extract 0.4 g/l DNA 0.2 g/l CMRL 1066 Fetal bovine serum Swine serum Distilled water
40782MEDIUM 79 - for MycoplasmayesDistilled water make up to (610.000 ml);Glucose (5.000 g);Peptone (5.300g);Tryptone (10.000 g);PPLO broth (0.500 g);Sterile supplement - M00265 (375.000 ml)

culture temp

@refgrowthtypetemperaturerange
8310positivegrowth37mesophilic
40782positivegrowth37mesophilic

Isolation, sampling and environmental information

isolation

  • @ref: 8310
  • sample type: urethra of male with non-gonococcal urethritis
  • country: USA
  • origin.country: USA
  • continent: North America

isolation source categories

Cat1Cat2Cat3
#Infection#Disease
#Infection#Patient
#Host Body-Site#Urogenital tract#Urethra
#Host#Human#Male

Safety information

risk assessment

  • @ref: 8310
  • biosafety level: 2
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Mycoplasma genitalium gene for 16S rRNA, partial sequenceAB069813251ena2097
20218Mycoplasma genitalium strain ATCC 33530 16S ribosomal RNA gene, partial sequence; 16S-23S ribosomal RNA intergenic spacer, complete sequence; and 23S ribosomal RNA gene, partial sequenceAY816341835ena2097
8310M.genitalium 16S rRNA geneX773341490ena2097

External links

@ref: 8310

culture collection no.: DSM 19775, ATCC 33530

straininfo link

  • @ref: 20218
  • passport: http://www.straininfo.net/strains/109438

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Metabolism23357772Contribution of topoisomerase IV mutation to quinolone resistance in Mycoplasma genitalium.Yamaguchi Y, Takei M, Kishii R, Yasuda M, Deguchi TAntimicrob Agents Chemother10.1128/AAC.01956-122013Anti-Bacterial Agents/*pharmacology, DNA Gyrase/genetics/metabolism, DNA Topoisomerase IV/*genetics/metabolism, Drug Resistance, Bacterial/genetics, Microbial Sensitivity Tests, Mutation, Mycoplasma genitalium/*drug effects/*enzymology/genetics, Polymerase Chain Reaction, Quinolones/*pharmacologyEnzymology
Pathogenicity25245990Sitafloxacin: antimicrobial activity against ciprofloxacin-selected laboratory mutants of Mycoplasma genitalium and inhibitory activity against its DNA gyrase and topoisomerase IV.Deguchi T, Kikuchi M, Yasuda M, Ito SJ Infect Chemother10.1016/j.jiac.2014.08.0212014Anti-Bacterial Agents/*pharmacology, Ciprofloxacin/pharmacology, DNA Gyrase/drug effects, DNA Topoisomerase IV/drug effects, DNA, Bacterial/genetics, Drug Resistance, Bacterial, Fluoroquinolones/*pharmacology, Humans, Microbial Sensitivity Tests, Mutation/*genetics, Mycoplasma Infections/microbiology, Mycoplasma genitalium/*drug effects/*genetics, Topoisomerase II Inhibitors/*pharmacology

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
8310Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 19775)https://www.dsmz.de/collection/catalogue/details/culture/DSM-19775
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
40782Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/15601