Strain identifier
BacDive ID: 8533
Type strain:
Species: Mycobacterium parascrofulaceum
Strain history: CIP <- 2003, C.Y. Turenne, Health Canada, Winnipeg, Manitoba, Canada: strain HSC68 <- J. Wolfe
NCBI tax ID(s): 240125 (species)
General
@ref: 11957
BacDive-ID: 8533
DSM-Number: 44648
keywords: genome sequence, 16S sequence, Bacteria, obligate aerobe, mesophilic
description: Mycobacterium parascrofulaceum DSM 44648 is an obligate aerobe, mesophilic bacterium that was isolated from cervix biopsy.
NCBI tax id
- NCBI tax id: 240125
- Matching level: species
strain history
@ref | history |
---|---|
11957 | <- C. Turenne; HSC 68 <- J. Wolfe |
67770 | KCTC 9979 <-- C. Y. Turenne HSC-68 <-- J. N. Wolfe. |
121594 | CIP <- 2003, C.Y. Turenne, Health Canada, Winnipeg, Manitoba, Canada: strain HSC68 <- J. Wolfe |
doi: 10.13145/bacdive8533.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/actinomycetota
- domain: Bacteria
- phylum: Actinomycetota
- class: Actinomycetes
- order: Mycobacteriales
- family: Mycobacteriaceae
- genus: Mycobacterium
- species: Mycobacterium parascrofulaceum
- full scientific name: Mycobacterium parascrofulaceum Turenne et al. 2004
@ref: 11957
domain: Bacteria
phylum: Actinobacteria
class: Actinobacteria
order: Actinomycetales
family: Mycobacteriaceae
genus: Mycobacterium
species: Mycobacterium parascrofulaceum
full scientific name: Mycobacterium parascrofulaceum Turenne et al. 2004 emend. Nouioui et al. 2018
type strain: yes
Morphology
cell morphology
@ref | motility | cell shape |
---|---|---|
30100 | no | |
121594 | no | rod-shaped |
pigmentation
- @ref: 30100
- production: yes
Culture and growth conditions
culture medium
@ref | name | growth | link | composition |
---|---|---|---|---|
11957 | MIDDLEBROOK MEDIUM (DSMZ Medium 645) | yes | https://mediadive.dsmz.de/medium/645 | Name: MIDDLEBROOK MEDIUM (DSMZ Medium 645) Composition: Bacto Middlebrook 7H10 agar 20.9945 g/l Glycerol Distilled water |
36338 | MEDIUM 55 - for Mycobacterium | yes | ||
121594 | CIP Medium 55 | yes | https://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=55 |
culture temp
@ref | growth | type | temperature | range |
---|---|---|---|---|
11957 | positive | growth | 37 | mesophilic |
30100 | positive | growth | 25-37 | mesophilic |
30100 | positive | optimum | 37 | mesophilic |
36338 | positive | growth | 37 | mesophilic |
67770 | positive | growth | 37 | mesophilic |
121594 | positive | growth | 30-37 | mesophilic |
121594 | no | growth | 10 | psychrophilic |
121594 | no | growth | 22 | psychrophilic |
121594 | no | growth | 45 | thermophilic |
Physiology and metabolism
oxygen tolerance
- @ref: 121594
- oxygen tolerance: obligate aerobe
halophily
- @ref: 30100
- salt: NaCl
- growth: positive
- tested relation: growth
- concentration: 5 %
metabolite utilization
@ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
---|---|---|---|---|
121594 | 606565 | hippurate | - | hydrolysis |
121594 | 17632 | nitrate | - | reduction |
121594 | 16301 | nitrite | - | reduction |
121594 | 17632 | nitrate | - | respiration |
metabolite production
- @ref: 121594
- Chebi-ID: 35581
- metabolite: indole
- production: no
enzymes
@ref | value | activity | ec |
---|---|---|---|
30100 | catalase | + | 1.11.1.6 |
30100 | pyrazinamidase | + | 3.5.1.B15 |
30100 | urease | + | 3.5.1.5 |
121594 | oxidase | - | |
121594 | beta-galactosidase | - | 3.2.1.23 |
121594 | gelatinase | - | |
121594 | catalase | + | 1.11.1.6 |
121594 | gamma-glutamyltransferase | + | 2.3.2.2 |
121594 | urease | + | 3.5.1.5 |
68382 | alkaline phosphatase | - | 3.1.3.1 |
68382 | esterase (C 4) | + | |
68382 | esterase lipase (C 8) | + | |
68382 | lipase (C 14) | - | |
68382 | leucine arylamidase | + | 3.4.11.1 |
68382 | valine arylamidase | + | |
68382 | cystine arylamidase | + | 3.4.11.3 |
68382 | trypsin | + | 3.4.21.4 |
68382 | alpha-chymotrypsin | - | 3.4.21.1 |
68382 | acid phosphatase | + | 3.1.3.2 |
68382 | naphthol-AS-BI-phosphohydrolase | + | |
68382 | alpha-galactosidase | - | 3.2.1.22 |
68382 | beta-galactosidase | - | 3.2.1.23 |
68382 | beta-glucuronidase | - | 3.2.1.31 |
68382 | alpha-glucosidase | + | 3.2.1.20 |
68382 | beta-glucosidase | - | 3.2.1.21 |
68382 | N-acetyl-beta-glucosaminidase | - | 3.2.1.52 |
68382 | alpha-mannosidase | - | 3.2.1.24 |
68382 | alpha-fucosidase | - | 3.2.1.51 |
API zym
@ref | Control | Alkaline phosphatase | Esterase | Esterase Lipase | Lipase | Leucine arylamidase | Valine arylamidase | Cystine arylamidase | Trypsin | alpha- Chymotrypsin | Acid phosphatase | Naphthol-AS-BI-phosphohydrolase | alpha- Galactosidase | beta- Galactosidase | beta- Glucuronidase | alpha- Glucosidase | beta- Glucosidase | N-acetyl-beta- glucosaminidase | alpha- Mannosidase | alpha- Fucosidase |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
121594 | - | - | + | + | - | + | + | + | + | - | + | + | - | - | - | + | - | - | - | - |
Isolation, sampling and environmental information
isolation
@ref | sample type | geographic location | country | origin.country | continent | isolation date |
---|---|---|---|---|---|---|
11957 | cervix biopsy | Winnipeg, Manitoba | Canada | CAN | North America | |
67770 | Cervix biopsy | Manitoba | Canada | CAN | North America | |
121594 | Human, Cervix biopsy | Manitoba | Canada | CAN | North America | 1981 |
isolation source categories
Cat1 | Cat2 | Cat3 |
---|---|---|
#Infection | #Patient | #Biopsy |
#Host Body-Site | #Urogenital tract | #Ovary |
Safety information
risk assessment
@ref | biosafety level | biosafety level comment |
---|---|---|
11957 | 2 | Risk group (German classification) |
121594 | 1 | Risk group (French classification) |
Sequence information
16S sequences
@ref | description | accession | length | database | NCBI tax ID |
---|---|---|---|---|---|
20218 | Mycobacterium parascrofulaceum strain BAA-614 16S ribosomal RNA gene, partial sequence; 16S-23S intergenic spacer region, complete sequence; and 23S ribosomal RNA gene, partial sequence | AY337279 | 373 | ena | 525368 |
20218 | Mycobacterium parascrofulaceum strain ATCC BAA-614 16S ribosomal RNA gene, partial sequence | GQ153273 | 1483 | ena | 525368 |
20218 | Mycobacterium parascrofulaceum strain ATCC BAA-614 16S ribosomal RNA gene, partial sequence | JF271815 | 1383 | ena | 525368 |
20218 | Mycobacterium parascrofulaceum strain ATCC BAA-614 16S ribosomal RNA gene, partial sequence; 16S-23S ribosomal RNA intergenic spacer, complete sequence; and 23S ribosomal RNA gene, partial sequence | JQ411511 | 441 | ena | 525368 |
20218 | Mycobacterium parascrofulaceum strain ATCC BAA-614 16S-23S ribosomal RNA intergenic spacer, partial sequence | JQ411531 | 281 | ena | 525368 |
20218 | Mycobacterium parascrofulaceum strain CIP 108112 16S ribosomal RNA gene, partial sequence | AY943211 | 539 | ena | 240125 |
30100 | Mycobacterium parascrofulaceum strain BAA-614 16S ribosomal RNA gene, partial sequence | AY337273 | 1468 | nuccore | 525368 |
Genome sequences
- @ref: 67770
- description: Mycobacterium parascrofulaceum ATCC BAA-614
- accession: GCA_000164135
- assembly level: scaffold
- database: ncbi
- NCBI tax ID: 525368
GC content
- @ref: 67770
- GC-content: 68.4
- method: genome sequence analysis
Genome-based predictions
predictions
trait | prediction | confidence | training_data |
---|---|---|---|
motile | no | 93.571 | yes |
flagellated | no | 98.708 | yes |
gram-positive | yes | 89.906 | no |
anaerobic | no | 98.738 | no |
aerobic | yes | 86.484 | no |
halophile | no | 92.673 | no |
spore-forming | no | 83.709 | no |
thermophile | no | 98.436 | yes |
glucose-util | yes | 65.316 | no |
glucose-ferment | no | 91.173 | no |
External links
@ref: 11957
culture collection no.: DSM 44648, ATCC BAA 614, HSC 68, JCM 13015, CIP 108112, KCTC 9979
straininfo link
- @ref: 77919
- straininfo: 100401
literature
- topic: Phylogeny
- Pubmed-ID: 15388708
- title: Mycobacterium parascrofulaceum sp. nov., novel slowly growing, scotochromogenic clinical isolates related to Mycobacterium simiae.
- authors: Turenne CY, Cook VJ, Burdz TV, Pauls RJ, Thibert L, Wolfe JN, Kabani A
- journal: Int J Syst Evol Microbiol
- DOI: 10.1099/ijs.0.02940-0
- year: 2004
- mesh: Adult, Aged, Anti-Bacterial Agents/pharmacology, Bacterial Proteins/genetics, Bacterial Typing Techniques, Bronchoalveolar Lavage Fluid/microbiology, Canada, Cervix Uteri/microbiology, Chaperonin 60, Chaperonins/genetics, DNA, Bacterial/chemistry/isolation & purification, DNA, Ribosomal/chemistry/isolation & purification, DNA, Ribosomal Spacer/chemistry/isolation & purification, Female, Genes, rRNA, Humans, Male, Microbial Sensitivity Tests, Middle Aged, Molecular Sequence Data, Mycobacterium Infections/*microbiology, Mycobacterium scrofulaceum/classification, Mycolic Acids/analysis, Nontuberculous Mycobacteria/*classification/genetics/*isolation & purification/physiology, Phylogeny, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Sputum/microbiology
- topic2: Enzymology
Reference
@id | authors | catalogue | doi/url | title | journal | pubmed | ID_cross_reference |
---|---|---|---|---|---|---|---|
11957 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 44648) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-44648 | ||||
20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | ||||
20218 | Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P. | 10.1016/j.syapm.2013.11.002 | StrainInfo introduces electronic passports for microorganisms. | Syst Appl Microbiol. 37: 42-50 2014 | 24321274 | ||
30100 | Barberan A, Caceres Velazquez H, Jones S, Fierer N. | 10.1128/mSphere.00237-17 | Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information | 28776041 | 26456 | ||
36338 | Curators of the CIP | https://brclims.pasteur.fr/brcWeb/souche/detail/1/5699 | |||||
67770 | Curators of the JCM | https://jcm.brc.riken.jp/en/ | |||||
68382 | Automatically annotated from API zym | ||||||
77919 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID100401.1 | StrainInfo: A central database for resolving microbial strain identifiers | ||||
121594 | Curators of the CIP | Collection of Institut Pasteur (CIP 108112) | https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20108112 |