Strain identifier

BacDive ID: 8530

Type strain: Yes

Species: Mycobacterium cosmeticum

Strain Designation: LTA-388

Culture col. no.: DSM 44829, ATCC BAA 878, CIP 108170, LTA-388 , CCUG 55485, JCM 14739

For citation purpose refer to the digital object identifier (doi) of the current version.
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General

@ref: 12011

BacDive-ID: 8530

DSM-Number: 44829

keywords: rod-shaped, mesophilic, aerobe, Bacteria, 16S sequence, genome sequence, human pathogen

description: Mycobacterium cosmeticum LTA-388 is an aerobe, mesophilic, rod-shaped human pathogen that was isolated from a granulomatous lesion of a female patient.

strain history: <- CIP

doi: 10.13145/bacdive8530.20201210.5

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinobacteria
  • domain: Bacteria
  • phylum: Actinobacteria
  • class: Actinobacteria
  • order: Mycobacteriales
  • family: Mycobacteriaceae
  • genus: Mycobacterium
  • species: Mycobacterium cosmeticum
  • full scientific name: Mycobacterium cosmeticum Cooksey et al. 2004
  • synonyms

    • @ref: 20215
    • synonym: Mycolicibacterium cosmeticum

@ref: 12011

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Mycobacteriaceae

genus: Mycobacterium

species: Mycobacterium cosmeticum

full scientific name: Mycobacterium cosmeticum Cooksey et al. 2004 emend. Nouioui et al. 2018

strain designation: LTA-388

type strain: yes

Morphology

cell morphology

  • @ref: 31297
  • cell length: 1.5 µm
  • cell width: 0.55 µm
  • cell shape: rod-shaped
  • motility: no

colony morphology

@refcolony colorincubation periodmedium used
20128Ivory (1014)10-14 days5006
20128Ivory (1014)10-14 daysBUG
20128Ivory (1014)10-14 daysMB7H9
606032 days

multimedia

  • @ref: 12011
  • multimedia content: https://www.dsmz.de/microorganisms/photos/DSM_44829.jpg
  • caption: Medium 645 37°C
  • license or copyright: (C) Leibniz-Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH
  • intellectual property rights: © Leibniz-Institut DSMZ

pigmentation

  • @ref: 31297
  • production: yes

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
12011MIDDLEBROOK MEDIUM (DSMZ Medium 645)yeshttps://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium645.pdf
20128BUGyes
201285006yesMedium: 5006 Name: Composition (g/l) Sucrose 3,0 Dextrin 15,0 Meat extract 1,0 Yeast extract 2,0 Tryptone soy broth (Oxoid) 5,0 NaCl 0,5 K2HPO4 0,5 MgSO4 x 7 H2O 0,5 FeSO4 x 7 H2O 0,01 Agar 20,0 Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7,3 Usage: Maintenance Organisms: All Actinomycetes
20128MB7H9yesName: Middlebrook 7H9 (MB7H9) Broth Base Composition: Middelbrook 7H9 Broth Base 4.7 g/l Glycerol 2.0 g/l Casaminoacids 11.0 g/l Yeast extract 5.5 g/l Tween80 (20%) 2.5 g/l OADC-Enrichment 100 ml Agar 15.0 g/l Preparation: Dissolve in Milli-Q-H2O. Before Use add 10% OADC stock solution Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Maintenance and revitalization Organisms: Mycobacteria OADC stock solution old broth-name: OADC-Stock Composition: Bovine Serum Albumin, fraction V 5 % 1% Oleic Acid in 0.2N NaOH 5ml Glucose 2 % NaCl 0.85 % Comments: Melt 1% Oleic Acid in 65°C water bath. Dissolve all ingredients in 700ml of ddH2O. Adjust volume to 1L. Filter sterilize and store at 4°C. -To make 1% Oleic Acid in 0.2N NaOH: Dissolve 5g oleic acid (ampule) in 500ml 0.2N NaOH (300ml ddH2O + 20ml NaOH + adjust volume to 500ml). Heat solution to 55°C for oleic acid to melt. Store at -20°C in 50ml aliquots. There is no need to sterilize this. Sterilisation: 20 minutes Temperature:121°C PH before: PH after: Biological details Culture type: suitable for: Mycobacteria
36388MEDIUM 55 - for Mycobacteriumyes

culture temp

@refgrowthtypetemperaturerange
12011positivegrowth37mesophilic
20128positiveoptimum37mesophilic
31297positiveoptimum35mesophilic
36388positivegrowth30mesophilic
60603positivegrowth30mesophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 60603
  • oxygen tolerance: aerobe

spore formation

  • @ref: 31297
  • spore formation: no

halophily

@refsaltgrowthtested relationconcentration
31297NaClpositivegrowth0-5 %
31297NaClpositiveoptimum2.5 %

observation

  • @ref: 31297
  • observation: aggregates in clumps

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
3129722599arabinose+carbon source
3129716947citrate+carbon source
3129729864mannitol+carbon source
3129727082trehalose+carbon source
3129718222xylose+carbon source
3129717632nitrate+reduction

enzymes

  • @ref: 31297
  • value: arylsulfatase
  • activity: +
  • ec: 3.1.6.1

API coryne

@refNITPYZPYRAPALbeta GURbeta GALalpha GLUbeta NAGESCUREGELGLURIBXYLMANMALLACSACGLYGControl
20128--------++-------+-
12011---+--+/---+----------

API zym

@refAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- FucosidaseControl
20128-++-+--+-------+---
12011-+/-+-++/-+/---+----------

Isolation, sampling and environmental information

isolation

@refsample typecountryorigin.countrycontinentsampling dategeographic location
12011a granulomatous lesion of a female patientVenezuelaVENMiddle and South America
60603Human granulomatous subdermal lesionVenezuelaVENMiddle and South America2003Caracas

isolation source categories

Cat1Cat2Cat3
#Infection#Inflammation
#Infection#Patient
#Host#Human#Female

Safety information

risk assessment

@refpathogenicity humanbiosafety levelbiosafety level comment
12011yes1Risk group (German classification)
201282German classification

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Mycobacterium cosmeticum strain DSM 44829 16S ribosomal RNA gene, partial sequenceDQ124114532ena258533
12011Mycobacterium cosmeticum strain LTA-388 16S ribosomal RNA gene, partial sequenceAY4497281507ena258533

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Mycolicibacterium cosmeticum DSM 44829GCA_000613185contigncbi258533
66792Mycolicibacterium cosmeticum DSM 44829GCA_005670675scaffoldncbi258533
66792Mycobacterium cosmeticum strain DSM 44829258533.3wgspatric258533
66792Mycolicibacterium cosmeticum strain DSM 44829 strain not applicable258533.7wgspatric258533

External links

@ref: 12011

culture collection no.: DSM 44829, ATCC BAA 878, CIP 108170, LTA-388, CCUG 55485, JCM 14739

straininfo link

@refpassport
20218http://www.straininfo.net/strains/381831
20218http://www.straininfo.net/strains/371028
20218http://www.straininfo.net/strains/381829
20218http://www.straininfo.net/strains/381830

Reference

@idauthorscataloguedoi/urltitlejournalpubmedID_cross_reference
12011Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 44829)https://www.dsmz.de/collection/catalogue/details/culture/DSM-44829
20128Wink, J.http://www.dsmz.de/microorganisms/wink_pdf/DSM44829.pdfCompendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig
20215D.Gleim, M.Kracht, N.Weiss et. al.http://www.dsmz.de/bacterial-diversity/prokaryotic-nomenclature-up-to-date.htmlProkaryotic Nomenclature Up-to-date - compilation of all names of Bacteria and Archaea, validly published according to the Bacteriological Code since 1. Jan. 1980, and validly published nomenclatural changes since
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
2761610.1099/ijs.0.63238-0
31297Barberan A, Caceres Velazquez H, Jones S, Fierer N.10.1128/mSphere.00237-17Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information27616
36388Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/5763
60603Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 55485)https://www.ccug.se/strain?id=55485
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)