Strain identifier

BacDive ID: 8523

Type strain: Yes

Species: Mycobacterium neglectum

Strain Designation: BN3150

Strain history: CIP <- 2003, R. Schulze-Röbbecke, Düsseldorf Univ., Düsseldorf, Germany: strain BN3150

NCBI tax ID(s): 242737 (species)

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General

@ref: 12065

BacDive-ID: 8523

DSM-Number: 44756

keywords: 16S sequence, Bacteria, facultative anaerobe, mesophilic, rod-shaped

description: Mycobacterium neglectum BN3150 is a facultative anaerobe, mesophilic, rod-shaped bacterium that was isolated from biofilm from underground drinking water distribution mains.

NCBI tax id

  • NCBI tax id: 242737
  • Matching level: species

strain history

@refhistory
12065<- R. Schulze-Röbbecke; BN3150 <- B. Ilg
121256CIP <- 2003, R. Schulze-Röbbecke, Düsseldorf Univ., Düsseldorf, Germany: strain BN3150

doi: 10.13145/bacdive8523.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Mycobacteriales
  • family: Mycobacteriaceae
  • genus: Mycobacterium
  • species: Mycobacterium neglectum
  • full scientific name: Mycobacterium neglectum Nouioui et al. 2019

@ref: 12065

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Mycobacteriaceae

genus: Mycobacterium

species: Mycobacterium neglectum

full scientific name: Mycobacterium neglectum Nouioui et al. 2019

strain designation: BN3150

type strain: yes

Morphology

cell morphology

  • @ref: 121256
  • cell shape: rod-shaped
  • motility: no

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
12065MIDDLEBROOK MEDIUM (DSMZ Medium 645)yeshttps://mediadive.dsmz.de/medium/645Name: MIDDLEBROOK MEDIUM (DSMZ Medium 645) Composition: Bacto Middlebrook 7H10 agar 20.9945 g/l Glycerol Distilled water
12065LÖWENSTEIN-JENSEN MEDIUM (DSMZ Medium 354)yeshttps://mediadive.dsmz.de/medium/354Name: LÖWENSTEIN-JENSEN MEDIUM (DSMZ Medium 354) Composition: Potato flour 18.6104 g/l L-Asparagin 2.23325 g/l KH2PO4 1.55087 g/l Mg-citrate 0.372208 g/l Malachite green 0.248139 g/l MgSO4 0.148883 g/l Glycerol Fresh egg mixture Distilled water
36339MEDIUM 55 - for Mycobacteriumyes
121256CIP Medium 55yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=55

culture temp

@refgrowthtypetemperaturerange
12065positivegrowth28mesophilic
36339positivegrowth21psychrophilic
121256positivegrowth10-30
121256nogrowth45thermophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 121256
  • oxygen tolerance: facultative anaerobe

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
121256606565hippurate-hydrolysis
12125617632nitrate+reduction
12125616301nitrite-reduction
12125617632nitrate+respiration

metabolite production

  • @ref: 121256
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

enzymes

@refvalueactivityec
121256oxidase-
121256beta-galactosidase-3.2.1.23
121256gelatinase-
121256amylase-
121256DNase-
121256caseinase-3.4.21.50
121256catalase+1.11.1.6
121256tween esterase-
121256gamma-glutamyltransferase+2.3.2.2
121256lecithinase-
121256lipase-
121256protease-
121256urease+3.5.1.5
68382alkaline phosphatase+3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)+
68382lipase (C 14)-
68382leucine arylamidase+3.4.11.1
68382valine arylamidase-
68382cystine arylamidase+3.4.11.3
68382trypsin+3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase-3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase+3.2.1.20
68382beta-glucosidase-3.2.1.21
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
121256-+++-+-++-++---+----

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
121256-+------------------+-----++----++--++-----------+------------+-+--------------++-------+----------

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentisolation date
12065biofilm from underground drinking water distribution mainsDuisburgGermanyDEUEurope
121256Environment, Biofilm from underground drinking waterDuisburgGermanyDEUEurope1999

isolation source categories

Cat1Cat2Cat3
#Environmental#Aquatic#Freshwater
#Environmental#Biofilm

Safety information

risk assessment

  • @ref: 121256
  • biosafety level: 1
  • biosafety level comment: Risk group (French classification)

Sequence information

16S sequences

  • @ref: 20218
  • description: Mycobacterium neglectum strain DSM 44756 16S ribosomal RNA gene, partial sequence
  • accession: DQ350151
  • length: 514
  • database: ena
  • NCBI tax ID: 242737

External links

@ref: 12065

culture collection no.: DSM 44756, CIP 108078

straininfo link

  • @ref: 77912
  • straininfo: 215525

literature

  • topic: Phylogeny
  • Pubmed-ID: 29404824
  • title: Formal description of Mycobacterium neglectum sp. nov. and Mycobacterium palauense sp. nov., rapidly growing actinobacteria.
  • authors: Nouioui I, Carro L, Sangal V, Jando M, Igual JM, Goodfellow M, Klenk HP
  • journal: Antonie Van Leeuwenhoek
  • DOI: 10.1007/s10482-018-1029-5
  • year: 2018
  • mesh: DNA, Bacterial/genetics, Multilocus Sequence Typing, Mycobacterium/*classification/genetics/growth & development/*isolation & purification, Nucleic Acid Hybridization, Phylogeny, RNA, Ribosomal, 16S/genetics
  • topic2: Enzymology

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
12065Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 44756)https://www.dsmz.de/collection/catalogue/details/culture/DSM-44756
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
36339Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/5660
68382Automatically annotated from API zym
77912Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID215525.1StrainInfo: A central database for resolving microbial strain identifiers
121256Curators of the CIPCollection of Institut Pasteur (CIP 108078)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20108078