Strain identifier
BacDive ID: 8521
Type strain:
Species: Mycobacterium brisbanense
Strain Designation: MF-614, W6743
Strain history: CIP <- 2003, G. Goolsby, CDC, Atlanta, Georgia, USA: strain W6743 <- R.J. Wallace <- J.M. Blacklock
NCBI tax ID(s): 146020 (species)
General
@ref: 11995
BacDive-ID: 8521
DSM-Number: 44680
keywords: genome sequence, 16S sequence, Bacteria, aerobe, mesophilic, Gram-positive
description: Mycobacterium brisbanense MF-614 is an aerobe, mesophilic, Gram-positive bacterium that was isolated from antral sinus.
NCBI tax id
- NCBI tax id: 146020
- Matching level: species
strain history
@ref | history |
---|---|
11995 | <- J. M. Brown, CDC; W6743 <- R. J. Wallace <- Z. M. Blacklock |
67770 | CIP 107830 <-- G. Goolsby W6743 <-- R. J. Wallace Mf-614 <-- J. M. Blacklock. |
117046 | CIP <- 2003, G. Goolsby, CDC, Atlanta, Georgia, USA: strain W6743 <- R.J. Wallace <- J.M. Blacklock |
doi: 10.13145/bacdive8521.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/actinomycetota
- domain: Bacteria
- phylum: Actinomycetota
- class: Actinomycetes
- order: Mycobacteriales
- family: Mycobacteriaceae
- genus: Mycobacterium
- species: Mycobacterium brisbanense
- full scientific name: Mycobacterium brisbanense Schinsky et al. 2004
synonyms
- @ref: 20215
- synonym: Mycolicibacterium brisbanense
@ref: 11995
domain: Bacteria
phylum: Actinobacteria
class: Actinobacteria
order: Actinomycetales
family: Mycobacteriaceae
genus: Mycobacterium
species: Mycobacterium brisbanense
full scientific name: Mycobacterium brisbanense Schinsky et al. 2004
strain designation: MF-614, W6743
type strain: yes
Morphology
cell morphology
@ref | gram stain | motility | confidence | cell shape |
---|---|---|---|---|
29958 | positive | |||
69480 | no | 92.515 | ||
69480 | positive | 100 | ||
117046 | no | rod-shaped |
colony morphology
- @ref: 57767
- incubation period: 2 days
Culture and growth conditions
culture medium
@ref | name | growth | link | composition |
---|---|---|---|---|
11995 | MIDDLEBROOK MEDIUM (DSMZ Medium 645) | yes | https://mediadive.dsmz.de/medium/645 | Name: MIDDLEBROOK MEDIUM (DSMZ Medium 645) Composition: Bacto Middlebrook 7H10 agar 20.9945 g/l Glycerol Distilled water |
36376 | MEDIUM 55 - for Mycobacterium | yes | ||
117046 | CIP Medium 72 | yes | https://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=72 | |
117046 | CIP Medium 55 | yes | https://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=55 |
culture temp
@ref | growth | type | temperature | range |
---|---|---|---|---|
11995 | positive | growth | 37 | mesophilic |
29958 | positive | growth | 28-35 | mesophilic |
29958 | positive | optimum | 31.5 | mesophilic |
36376 | positive | growth | 37 | mesophilic |
57767 | positive | growth | 37-42 | |
67770 | positive | growth | 37 | mesophilic |
117046 | positive | growth | 10-30 | |
117046 | no | growth | 45 | thermophilic |
Physiology and metabolism
oxygen tolerance
- @ref: 57767
- oxygen tolerance: aerobe
spore formation
@ref | spore formation | confidence |
---|---|---|
29958 | no | |
69481 | no | 99 |
69480 | no | 99.257 |
halophily
- @ref: 29958
- salt: NaCl
- growth: positive
- tested relation: optimum
- concentration: 5 %
metabolite utilization
@ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
---|---|---|---|---|
29958 | 15963 | ribitol | + | carbon source |
29958 | 22599 | arabinose | + | carbon source |
29958 | 28757 | fructose | + | carbon source |
29958 | 17234 | glucose | + | carbon source |
29958 | 29864 | mannitol | + | carbon source |
29958 | 37684 | mannose | + | carbon source |
29958 | 17268 | myo-inositol | + | carbon source |
29958 | 17814 | salicin | + | carbon source |
29958 | 30911 | sorbitol | + | carbon source |
29958 | 27082 | trehalose | + | carbon source |
29958 | 17632 | nitrate | + | reduction |
117046 | 606565 | hippurate | + | hydrolysis |
117046 | 17632 | nitrate | - | reduction |
117046 | 16301 | nitrite | - | reduction |
antibiotic resistance
- @ref: 117046
- metabolite: 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)
- is antibiotic: yes
- is sensitive: yes
- is resistant: no
metabolite production
- @ref: 117046
- Chebi-ID: 35581
- metabolite: indole
- production: no
enzymes
@ref | value | activity | ec |
---|---|---|---|
29958 | arylsulfatase | + | 3.1.6.1 |
29958 | catalase | + | 1.11.1.6 |
29958 | urease | + | 3.5.1.5 |
117046 | oxidase | - | |
117046 | beta-galactosidase | - | 3.2.1.23 |
117046 | gelatinase | - | |
117046 | amylase | - | |
117046 | DNase | + | |
117046 | caseinase | - | 3.4.21.50 |
117046 | catalase | + | 1.11.1.6 |
117046 | tween esterase | + | |
117046 | gamma-glutamyltransferase | + | 2.3.2.2 |
117046 | lecithinase | - | |
117046 | lipase | - | |
117046 | protease | - | |
117046 | urease | + | 3.5.1.5 |
68382 | alkaline phosphatase | - | 3.1.3.1 |
68382 | esterase (C 4) | + | |
68382 | esterase lipase (C 8) | + | |
68382 | lipase (C 14) | - | |
68382 | leucine arylamidase | + | 3.4.11.1 |
68382 | valine arylamidase | - | |
68382 | cystine arylamidase | - | 3.4.11.3 |
68382 | trypsin | - | 3.4.21.4 |
68382 | alpha-chymotrypsin | - | 3.4.21.1 |
68382 | acid phosphatase | + | 3.1.3.2 |
68382 | naphthol-AS-BI-phosphohydrolase | - | |
68382 | alpha-galactosidase | - | 3.2.1.22 |
68382 | beta-galactosidase | - | 3.2.1.23 |
68382 | beta-glucuronidase | - | 3.2.1.31 |
68382 | alpha-glucosidase | - | 3.2.1.20 |
68382 | beta-glucosidase | + | 3.2.1.21 |
68382 | N-acetyl-beta-glucosaminidase | - | 3.2.1.52 |
68382 | alpha-mannosidase | - | 3.2.1.24 |
68382 | alpha-fucosidase | - | 3.2.1.51 |
API zym
@ref | Control | Alkaline phosphatase | Esterase | Esterase Lipase | Lipase | Leucine arylamidase | Valine arylamidase | Cystine arylamidase | Trypsin | alpha- Chymotrypsin | Acid phosphatase | Naphthol-AS-BI-phosphohydrolase | alpha- Galactosidase | beta- Galactosidase | beta- Glucuronidase | alpha- Glucosidase | beta- Glucosidase | N-acetyl-beta- glucosaminidase | alpha- Mannosidase | alpha- Fucosidase |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
117046 | - | - | + | + | - | + | - | - | - | - | + | - | - | - | - | - | + | - | - | - |
API biotype100
@ref | GLU | FRU | GAL | TRE | MNE | SBE | MEL | SAC | RAF | MTE | MAL | LAC | LTE | MbGa | MaGa | CEL | GEN | MbGu | ESC | RIB | ARA | XYL | PLE | RHA | FUC | MLZ | DARL | LARL | XLT | DUL | TAG | GLY | INO | MAN | MTL | TUR | SOR | ADO | HBG | LYX | ERY | MDG | 3MDG | SAT | MUC | LTAT | DTAT | MTAT | DMLT | LMLT | CATE | TATE | TTE | CIT | GRT | GAT | 2KG | 5KG | TRY | NAG | GNT | PAC | PAT | pOBE | QAT | GTE | mOBE | BAT | PPAT | CMT | TGE | BET | PCE | ABT | HIN | LAT | CAP | CYT | HIS | SUC | FUM | GRE | GYT | AVT | ETN | TTN | GLN | ITA | 3OBU | APT | GTT | PRO | DALA | LALA | SER | MNT | PROP | TYR | 2KT |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
117046 | + | + | - | + | - | + | - | - | - | - | - | - | - | - | - | - | - | - | - | + | - | - | - | + | - | - | + | + | + | - | - | + | + | + | - | - | + | + | - | - | + | - | - | - | - | - | - | - | - | + | - | - | - | - | - | - | + | - | - | + | + | - | + | - | + | - | - | + | - | - | + | + | + | + | + | + | - | + | - | - | + | - | - | + | + | - | + | - | + | - | + | + | + | + | + | - | + | - | - |
Isolation, sampling and environmental information
isolation
@ref | sample type | geographic location | country | origin.country | continent |
---|---|---|---|---|---|
11995 | antral sinus | Brisbane | Australia | AUS | Australia and Oceania |
57767 | Antral sinus | Brisbane | Australia | AUS | Australia and Oceania |
67770 | Antral sinus | Brisbane | Australia | AUS | Australia and Oceania |
117046 | Antral sinus | Australia | AUS | Australia and Oceania |
isolation source categories
- Cat1: #Host Body-Site
- Cat2: #Oral cavity and airways
taxonmaps
- @ref: 69479
- File name: preview.99_1906.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_10;96_115;97_219;98_248;99_1906&stattab=map
- Last taxonomy: Mycolicibacterium
- 16S sequence: AY012577
- Sequence Identity:
- Total samples: 640
- soil counts: 361
- aquatic counts: 128
- animal counts: 69
- plant counts: 82
Safety information
risk assessment
@ref | biosafety level | biosafety level comment |
---|---|---|
11995 | 2 | Risk group (German classification) |
117046 | 1 | Risk group (French classification) |
Sequence information
16S sequences
- @ref: 11995
- description: Mycobacterium brisbanense strain W6743 16S ribosomal RNA gene, partial sequence
- accession: AY012577
- length: 1499
- database: ena
- NCBI tax ID: 146020
Genome sequences
@ref | description | accession | assembly level | database | NCBI tax ID |
---|---|---|---|---|---|
66792 | Mycobacterium brisbanense strain JCM15654 | 146020.3 | wgs | patric | 146020 |
67770 | Mycolicibacterium brisbanense JCM15654 | GCA_001570425 | scaffold | ncbi | 146020 |
66792 | Mycolicibacterium brisbanense DSM 44680 | GCA_025822265 | scaffold | ncbi | 146020 |
GC content
@ref | GC-content | method |
---|---|---|
29958 | 62 | |
67770 | 62 | thermal denaturation, midpoint method (Tm) |
Genome-based predictions
predictions
trait | prediction | confidence | training_data |
---|---|---|---|
spore-forming | no | 99 | no |
motile | no | 92.743 | no |
gram-positive | yes | 87.716 | yes |
anaerobic | no | 98.813 | yes |
halophile | no | 95.856 | no |
spore-forming | no | 82.875 | no |
thermophile | no | 99.575 | yes |
glucose-util | yes | 89.192 | yes |
aerobic | yes | 90.968 | yes |
flagellated | no | 97.592 | no |
glucose-ferment | no | 90.983 | no |
External links
@ref: 11995
culture collection no.: DSM 44680, ATCC 49938, CCUG 47584, CIP 107830, JCM 15654
straininfo link
- @ref: 77910
- straininfo: 42765
literature
topic | Pubmed-ID | title | authors | journal | DOI | year | mesh | topic2 |
---|---|---|---|---|---|---|---|---|
Phylogeny | 15388725 | Taxonomic variation in the Mycobacterium fortuitum third biovariant complex: description of Mycobacterium boenickei sp. nov., Mycobacterium houstonense sp. nov., Mycobacterium neworleansense sp. nov. and Mycobacterium brisbanense sp. nov. and recognition of Mycobacterium porcinum from human clinical isolates. | Schinsky MF, Morey RE, Steigerwalt AG, Douglas MP, Wilson RW, Floyd MM, Butler WR, Daneshvar MI, Brown-Elliott BA, Wallace RJ, McNeil MM, Brenner DJ, Brown JM | Int J Syst Evol Microbiol | 10.1099/ijs.0.02743-0 | 2004 | Anti-Bacterial Agents/pharmacology, Bacterial Typing Techniques, Base Composition, Chaperonin 60/genetics, DNA Fingerprinting, DNA, Bacterial/chemistry/isolation & purification, DNA, Ribosomal/chemistry/isolation & purification, Fatty Acids/analysis, Genes, rRNA/genetics, Humans, Microbial Sensitivity Tests, Molecular Sequence Data, Mycobacterium Infections, Nontuberculous/*microbiology, Mycobacterium fortuitum/*classification/genetics/isolation & purification/physiology, Mycolic Acids/analysis, Nontuberculous Mycobacteria/*classification/genetics/isolation & purification/physiology, Nucleic Acid Hybridization, Phylogeny, Polymorphism, Restriction Fragment Length, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/genetics, Ribotyping, Sequence Analysis, DNA | Enzymology |
Phylogeny | 24687707 | Mycobacterium brisbanense species nova isolated from a patient with chronic cavitary lung infection. | Poh ME, Liam CK, Ng KP, Tan R | Chest | 10.1378/chest.13-1952 | 2014 | Chronic Disease, Humans, Male, Middle Aged, Mycobacterium Infections, Nontuberculous/*microbiology, Nontuberculous Mycobacteria/*isolation & purification, Pneumonia, Bacterial/*microbiology | Pathogenicity |
Reference
@id | authors | catalogue | doi/url | title | ID_cross_reference | pubmed | journal |
---|---|---|---|---|---|---|---|
11995 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 44680) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-44680 | ||||
20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | ||||
29958 | Barberan A, Caceres Velazquez H, Jones S, Fierer N. | 10.1128/mSphere.00237-17 | Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information | 26327 | 28776041 | ||
36376 | Curators of the CIP | https://brclims.pasteur.fr/brcWeb/souche/detail/1/5385 | |||||
57767 | Curators of the CCUG | Culture Collection University of Gothenburg (CCUG) (CCUG 47584) | https://www.ccug.se/strain?id=47584 | ||||
66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | ||||
66794 | Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg | 10.1093/nar/gkaa1025 | BRENDA, the ELIXIR core data resource in 2021: new developments and updates | 33211880 | Nucleic Acids Res. 49: D498-D508 2020 | ||
67770 | Curators of the JCM | https://jcm.brc.riken.jp/en/ | |||||
68382 | Automatically annotated from API zym | ||||||
69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | https://microbeatlas.org/ | MicrobeAtlas 1.0 beta | ||||
69480 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | ||||
69481 | Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch | 10.21203/rs.3.rs-2527258/v1 | Predictions based on the model GenomeNet Sporulation v. 1 | ||||
77910 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID42765.1 | StrainInfo: A central database for resolving microbial strain identifiers | ||||
117046 | Curators of the CIP | Collection of Institut Pasteur (CIP 107830) | https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20107830 |