Strain identifier

BacDive ID: 8520

Type strain: Yes

Species: Mycobacterium neworleansense

Strain Designation: MF-465, W6705

Strain history: CIP <- 2003, G. Goolsby, CDC, Atlanta, USA: strain W6705 <- 1997, R.J. Wallace, Univ. Texas Hlth. Cent., Tyler, Texas, USA: strain MF 465, Mycobacterium fortuitum <- G. Bedsole <- Charity Hosp., New Orleans, LA, USA

NCBI tax ID(s): 146018 (species)

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General

@ref: 11994

BacDive-ID: 8520

DSM-Number: 44679

keywords: genome sequence, 16S sequence, Bacteria, aerobe, mesophilic, Gram-positive

description: Mycobacterium neworleansense MF-465 is an aerobe, mesophilic, Gram-positive bacterium that was isolated from scalp wound.

NCBI tax id

  • NCBI tax id: 146018
  • Matching level: species

strain history

@refhistory
11994<- J. M. Brown, CDC; W6705 <- R. J. Wallace <- G. Bedsole <- Charity Hospital, New Orleans
67770CIP 107827 <-- G. Goolsby W6705 <-- R. J. Wallace Mf-465 <-- G. Bedsole <-- Charity Hosp., New Orleans, USA.
121284CIP <- 2003, G. Goolsby, CDC, Atlanta, USA: strain W6705 <- 1997, R.J. Wallace, Univ. Texas Hlth. Cent., Tyler, Texas, USA: strain MF 465, Mycobacterium fortuitum <- G. Bedsole <- Charity Hosp., New Orleans, LA, USA

doi: 10.13145/bacdive8520.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Mycobacteriales
  • family: Mycobacteriaceae
  • genus: Mycobacterium
  • species: Mycobacterium neworleansense
  • full scientific name: Mycobacterium neworleansense Schinsky et al. 2004

  • synonyms

    • @ref: 20215
    • synonym: Mycolicibacterium neworleansense

@ref: 11994

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Mycobacteriaceae

genus: Mycobacterium

species: Mycobacterium neworleansense

full scientific name: Mycobacterium neworleansense Schinsky et al. 2004 emend. Nouioui et al. 2018

strain designation: MF-465, W6705

type strain: yes

Morphology

cell morphology

@refgram staincell shapemotility
29958positive
121284rod-shapedno

colony morphology

@refcolony colorincubation periodmedium used
20093Light ivory (1015)10-14 days5006
20093Light ivory (1015)10-14 daysMB7H10
20093Light ivory (1015)10-14 daysMB7H11
577652 days

multimedia

  • @ref: 11994
  • multimedia content: https://www.dsmz.de/microorganisms/photos/DSM_44679.jpg
  • caption: Medium 645 37°C
  • intellectual property rights: © Leibniz-Institut DSMZ

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
11994MYCOBACTERIUM MEDIUM (DSMZ Medium 219)yeshttps://mediadive.dsmz.de/medium/219Name: MYCOBACTERIUM MEDIUM (DSMZ Medium 219) Composition: Agar 20.0 g/l Na2HPO4 x 12 H2O 2.5 g/l Yeast extract 2.0 g/l Proteose peptone no. 3 2.0 g/l Casein peptone 2.0 g/l Sodium citrate 1.5 g/l KH2PO4 1.0 g/l MgSO4 x 7 H2O 0.6 g/l Tween 0.5 g/l Glycerol Distilled water
11994MIDDLEBROOK MEDIUM (DSMZ Medium 645)yeshttps://mediadive.dsmz.de/medium/645Name: MIDDLEBROOK MEDIUM (DSMZ Medium 645) Composition: Bacto Middlebrook 7H10 agar 20.9945 g/l Glycerol Distilled water
20093MB7H10yesName: Middlebrook 7H10 (MB7H10) Broth Base Composition: amount in percent Middlebrook 7H10 Agar 1.9% Glycerol 0.5% OADC-Enrichment in ml (NL5633) 10ml Comments: Add broth base and glycerol to ddh2O. Adjust volumes. Autoclave. Cool to 42°C. Add OADC-stock. Sterilisation: 20 minutes Temperature:121°C PH before: PH after: Biological details Culture type: suitable for: Mycobacteria OADC stock solution old broth-name: OADC-Stock Composition: Bovine Serum Albumin, fraction V 5 % 1% Oleic Acid in 0.2N NaOH 5ml Glucose 2 % NaCl 0.85 % Comments: Melt 1% Oleic Acid in 65°C water bath. Dissolve all ingredients in 700ml of ddH2O. Adjust volume to 1L. Filter sterilize and store at 4°C. -To make 1% Oleic Acid in 0.2N NaOH: Dissolve 5g oleic acid (ampule) in 500ml 0.2N NaOH (300ml ddH2O + 20ml NaOH + adjust volume to 500ml). Heat solution to 55°C for oleic acid to melt. Store at -20°C in 50ml aliquots. There is no need to sterilize this. Sterilisation: 20 minutes Temperature:121°C PH before: PH after: Biological details Culture type: suitable for: Mycobacteria
20093MB7H11yesName: Middlebrook 7H11 (MB7H11) Broth Base Composition: Middlebrook 7H11 Agar 2% Glycerol 0.5% OADC-Enrichment 10ml Comments: Add broth base and glycerol to ddH2O. Adjust volumes. Autoclave. Cool to 42°C. Add OADC-Stock. Sterilisation: 20 minutes Temperature:121°C PH before: PH after: Biological details Culture type: suitable for: Mycobacteria OADC stock solution old broth-name: OADC-Stock Composition: Bovine Serum Albumin, fraction V 5 % 1% Oleic Acid in 0.2N NaOH 5ml Glucose 2 % NaCl 0.85 % Comments: Melt 1% Oleic Acid in 65°C water bath. Dissolve all ingredients in 700ml of ddH2O. Adjust volume to 1L. Filter sterilize and store at 4°C. -To make 1% Oleic Acid in 0.2N NaOH: Dissolve 5g oleic acid (ampule) in 500ml 0.2N NaOH (300ml ddH2O + 20ml NaOH + adjust volume to 500ml). Heat solution to 55°C for oleic acid to melt. Store at -20°C in 50ml aliquots. There is no need to sterilize this. Sterilisation: 20 minutes Temperature:121°C PH before: PH after: Biological details Culture type: suitable for: Mycobacteria
200935006yesMedium: 5006 Name: Composition (g/l) Sucrose 3,0 Dextrin 15,0 Meat extract 1,0 Yeast extract 2,0 Tryptone soy broth (Oxoid) 5,0 NaCl 0,5 K2HPO4 0,5 MgSO4 x 7 H2O 0,5 FeSO4 x 7 H2O 0,01 Agar 20,0 Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7,3 Usage: Maintenance Organisms: All Actinomycetes
38235MEDIUM 55 - for Mycobacteriumyes
121284CIP Medium 55yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=55
121284CIP Medium 72yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=72

culture temp

@refgrowthtypetemperaturerange
20093positiveoptimum37mesophilic
11994positivegrowth37mesophilic
29958positivegrowth28-35mesophilic
29958positiveoptimum31.5mesophilic
38235positivegrowth37mesophilic
57765positivegrowth37mesophilic
67770positivegrowth37mesophilic
121284positivegrowth22-30
121284nogrowth10psychrophilic
121284nogrowth45thermophilic

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
57765aerobe
121284obligate aerobe

spore formation

  • @ref: 29958
  • spore formation: no

halophily

  • @ref: 29958
  • salt: NaCl
  • growth: positive
  • tested relation: optimum
  • concentration: 5 %

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
2995828757fructose+carbon source
2995817234glucose+carbon source
2995829864mannitol+carbon source
2995837684mannose+carbon source
2995817268myo-inositol+carbon source
2995817814salicin+carbon source
2995827082trehalose+carbon source
2995818222xylose+carbon source
2995817632nitrate+reduction
121284606565hippurate-hydrolysis
12128417632nitrate+reduction
12128416301nitrite-reduction
12128417632nitrate-respiration
6837917632nitrate-reduction
683794853esculin+hydrolysis
6837916199urea+hydrolysis
683795291gelatin-hydrolysis
6837917634D-glucose-fermentation
6837916988D-ribose-fermentation
6837965327D-xylose-fermentation
6837916899D-mannitol-fermentation
6837917306maltose-fermentation
6837917716lactose-fermentation
6837917992sucrose-fermentation
6837928087glycogen-fermentation

metabolite production

  • @ref: 121284
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

enzymes

@refvalueactivityec
29958arylsulfatase+3.1.6.1
29958catalase+1.11.1.6
29958urease+3.5.1.5
68379gelatinase-
68379urease+3.5.1.5
68379beta-glucosidase+3.2.1.21
68379N-acetyl-beta-glucosaminidase-3.2.1.52
68379alpha-glucosidase-3.2.1.20
68379beta-galactosidase-3.2.1.23
68379beta-glucuronidase-3.2.1.31
68379alkaline phosphatase-3.1.3.1
68379pyrrolidonyl arylamidase-3.4.19.3
68379pyrazinamidase+3.5.1.B15
121284oxidase-
121284beta-galactosidase-3.2.1.23
121284gelatinase-
121284amylase-
121284DNase-
121284caseinase-3.4.21.50
121284catalase+1.11.1.6
121284tween esterase+
121284gamma-glutamyltransferase+2.3.2.2
121284lecithinase-
121284lipase-
121284protease-
121284urease+3.5.1.5
68382alkaline phosphatase-3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)+
68382lipase (C 14)-
68382leucine arylamidase+3.4.11.1
68382valine arylamidase-
68382trypsin-3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase-3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase-3.2.1.20
68382beta-glucosidase+3.2.1.21
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-fucosidase-3.2.1.51

API coryne

@refNITPYZPYRAPALbeta GURbeta GALalpha GLUbeta NAGESCUREGELGLURIBXYLMANMALLACSACGLYG
20093-+------++---------

API zym

@refAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- FucosidaseControl
20093-++-+-+--++----+-+-
121284-++-+----++----+----

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
121284-+----------------+------------+++---------+-----+++--------+-----------+++-----+--++---+--++++-+-+

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentsampling dateisolation date
11994scalp woundLA, New OrleansUSAUSANorth America
57765Human wound,scalpNew-Orleans,Charity HospitalUSAUSANorth America1987
67770Scalp woundNew Orleans, LAUSAUSANorth America
121284Scalp woundNew Orleans, LouisianaUnited States of AmericaUSANorth America1987

isolation source categories

Cat1Cat2Cat3
#Host Body-Site#Other#Wound
#Host Body-Site#Organ#Skin, Nail, Hair

taxonmaps

  • @ref: 69479
  • File name: preview.99_515.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_10;96_115;97_219;98_419;99_515&stattab=map
  • Last taxonomy: Mycolicibacterium
  • 16S sequence: AY012575
  • Sequence Identity:
  • Total samples: 496
  • soil counts: 45
  • aquatic counts: 64
  • animal counts: 50
  • plant counts: 337

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
119942Risk group (German classification)
200932German classification
1212841Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Mycobacterium fortuitum strain ATCC 49404 16S ribosomal RNA gene, partial sequenceAF4805811470ena1766
20218Mycobacterium neworleansense strain ATCC 49404 16S ribosomal RNA gene, partial sequenceAY4570681483ena146018
20218M.fortuitum 16S rRNA (ATCC 49404)X65529885ena1766
11994Mycobacterium neworleansense strain W6705 16S ribosomal RNA gene, partial sequenceAY0125751480ena146018

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Mycolicibacterium neworleansense ATCC 494042690315792draftimg146018
67770Mycolicibacterium neworleansense ATCC 49404TGCA_001245615contigncbi146018
66792Mycolicibacterium neworleansense DSM 44679GCA_025823025scaffoldncbi146018

GC content

@refGC-contentmethod
2995860
6777066.8genome sequence analysis
6777060thermal denaturation, midpoint method (Tm)

External links

@ref: 11994

culture collection no.: CCUG 47582, CIP 107827, CIP 105354, DSM 44679, ATCC 49404, CDC 87-1098, JCM 15659

straininfo link

  • @ref: 77909
  • straininfo: 45216

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny15388725Taxonomic variation in the Mycobacterium fortuitum third biovariant complex: description of Mycobacterium boenickei sp. nov., Mycobacterium houstonense sp. nov., Mycobacterium neworleansense sp. nov. and Mycobacterium brisbanense sp. nov. and recognition of Mycobacterium porcinum from human clinical isolates.Schinsky MF, Morey RE, Steigerwalt AG, Douglas MP, Wilson RW, Floyd MM, Butler WR, Daneshvar MI, Brown-Elliott BA, Wallace RJ, McNeil MM, Brenner DJ, Brown JMInt J Syst Evol Microbiol10.1099/ijs.0.02743-02004Anti-Bacterial Agents/pharmacology, Bacterial Typing Techniques, Base Composition, Chaperonin 60/genetics, DNA Fingerprinting, DNA, Bacterial/chemistry/isolation & purification, DNA, Ribosomal/chemistry/isolation & purification, Fatty Acids/analysis, Genes, rRNA/genetics, Humans, Microbial Sensitivity Tests, Molecular Sequence Data, Mycobacterium Infections, Nontuberculous/*microbiology, Mycobacterium fortuitum/*classification/genetics/isolation & purification/physiology, Mycolic Acids/analysis, Nontuberculous Mycobacteria/*classification/genetics/isolation & purification/physiology, Nucleic Acid Hybridization, Phylogeny, Polymorphism, Restriction Fragment Length, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/genetics, Ribotyping, Sequence Analysis, DNAEnzymology
Genetics26543131Draft Genome Sequence of Mycobacterium neworleansense Strain ATCC 49404T.Asmar S, Robert C, Croce O, Caputo A, Drancourt MGenome Announc10.1128/genomeA.01314-152015

Reference

@idauthorscataloguedoi/urltitlejournalpubmedID_cross_reference
11994Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 44679)https://www.dsmz.de/collection/catalogue/details/culture/DSM-44679
20093Wink, J.http://www.dsmz.de/microorganisms/wink_pdf/DSM44679.pdfCompendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
29958Barberan A, Caceres Velazquez H, Jones S, Fierer N.10.1128/mSphere.00237-17Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information2877604126327
38235Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/5381
57765Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 47582)https://www.ccug.se/strain?id=47582
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68379Automatically annotated from API Coryne
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
77909Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID45216.1StrainInfo: A central database for resolving microbial strain identifiers
121284Curators of the CIPCollection of Institut Pasteur (CIP 107827)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20107827