Strain identifier

BacDive ID: 8520

Type strain: Yes

Species: Mycobacterium neworleansense

Strain Designation: MF-465, W6705

Culture col. no.: CCUG 47582, CIP 107827, CIP 105354, DSM 44679, ATCC 49404, CDC 87-1098

For citation purpose refer to the digital object identifier (doi) of the current version.
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General

@ref: 11994

BacDive-ID: 8520

DSM-Number: 44679

keywords: mesophilic, aerobe, Bacteria, 16S sequence, genome sequence, gram-positive

description: Mycobacterium neworleansense MF-465 is an aerobe, mesophilic, gram-positive bacterium that was isolated from scalp wound.

strain history: <- J. M. Brown, CDC; W6705 <- R. J. Wallace <- G. Bedsole <- Charity Hospital, New Orleans

doi: 10.13145/bacdive8520.20201210.5

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinobacteria
  • domain: Bacteria
  • phylum: Actinobacteria
  • class: Actinobacteria
  • order: Mycobacteriales
  • family: Mycobacteriaceae
  • genus: Mycobacterium
  • species: Mycobacterium neworleansense
  • full scientific name: Mycobacterium neworleansense Schinsky et al. 2004
  • synonyms

    • @ref: 20215
    • synonym: Mycolicibacterium neworleansense

@ref: 11994

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Mycobacteriaceae

genus: Mycobacterium

species: Mycobacterium neworleansense

full scientific name: Mycobacterium neworleansense Schinsky et al. 2004 emend. Nouioui et al. 2018

strain designation: MF-465, W6705

type strain: yes

Morphology

cell morphology

  • @ref: 29958
  • gram stain: positive

colony morphology

@refcolony colorincubation periodmedium used
20093Light ivory (1015)10-14 days5006
20093Light ivory (1015)10-14 daysMB7H10
20093Light ivory (1015)10-14 daysMB7H11
577652 days

multimedia

  • @ref: 11994
  • multimedia content: https://www.dsmz.de/microorganisms/photos/DSM_44679.jpg
  • caption: Medium 645 37°C
  • license or copyright: (C) Leibniz-Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH
  • intellectual property rights: © Leibniz-Institut DSMZ

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
11994MYCOBACTERIUM MEDIUM (DSMZ Medium 219)yeshttps://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium219.pdf
11994MIDDLEBROOK MEDIUM (DSMZ Medium 645)yeshttps://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium645.pdf
20093MB7H10yesName: Middlebrook 7H10 (MB7H10) Broth Base Composition: amount in percent Middlebrook 7H10 Agar 1.9% Glycerol 0.5% OADC-Enrichment in ml (NL5633) 10ml Comments: Add broth base and glycerol to ddh2O. Adjust volumes. Autoclave. Cool to 42°C. Add OADC-stock. Sterilisation: 20 minutes Temperature:121°C PH before: PH after: Biological details Culture type: suitable for: Mycobacteria OADC stock solution old broth-name: OADC-Stock Composition: Bovine Serum Albumin, fraction V 5 % 1% Oleic Acid in 0.2N NaOH 5ml Glucose 2 % NaCl 0.85 % Comments: Melt 1% Oleic Acid in 65°C water bath. Dissolve all ingredients in 700ml of ddH2O. Adjust volume to 1L. Filter sterilize and store at 4°C. -To make 1% Oleic Acid in 0.2N NaOH: Dissolve 5g oleic acid (ampule) in 500ml 0.2N NaOH (300ml ddH2O + 20ml NaOH + adjust volume to 500ml). Heat solution to 55°C for oleic acid to melt. Store at -20°C in 50ml aliquots. There is no need to sterilize this. Sterilisation: 20 minutes Temperature:121°C PH before: PH after: Biological details Culture type: suitable for: Mycobacteria
20093MB7H11yesName: Middlebrook 7H11 (MB7H11) Broth Base Composition: Middlebrook 7H11 Agar 2% Glycerol 0.5% OADC-Enrichment 10ml Comments: Add broth base and glycerol to ddH2O. Adjust volumes. Autoclave. Cool to 42°C. Add OADC-Stock. Sterilisation: 20 minutes Temperature:121°C PH before: PH after: Biological details Culture type: suitable for: Mycobacteria OADC stock solution old broth-name: OADC-Stock Composition: Bovine Serum Albumin, fraction V 5 % 1% Oleic Acid in 0.2N NaOH 5ml Glucose 2 % NaCl 0.85 % Comments: Melt 1% Oleic Acid in 65°C water bath. Dissolve all ingredients in 700ml of ddH2O. Adjust volume to 1L. Filter sterilize and store at 4°C. -To make 1% Oleic Acid in 0.2N NaOH: Dissolve 5g oleic acid (ampule) in 500ml 0.2N NaOH (300ml ddH2O + 20ml NaOH + adjust volume to 500ml). Heat solution to 55°C for oleic acid to melt. Store at -20°C in 50ml aliquots. There is no need to sterilize this. Sterilisation: 20 minutes Temperature:121°C PH before: PH after: Biological details Culture type: suitable for: Mycobacteria
200935006yesMedium: 5006 Name: Composition (g/l) Sucrose 3,0 Dextrin 15,0 Meat extract 1,0 Yeast extract 2,0 Tryptone soy broth (Oxoid) 5,0 NaCl 0,5 K2HPO4 0,5 MgSO4 x 7 H2O 0,5 FeSO4 x 7 H2O 0,01 Agar 20,0 Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7,3 Usage: Maintenance Organisms: All Actinomycetes
38235MEDIUM 55 - for Mycobacteriumyes

culture temp

@refgrowthtypetemperaturerange
20093positiveoptimum37mesophilic
11994positivegrowth37mesophilic
29958positivegrowth28-35mesophilic
29958positiveoptimum31.5mesophilic
38235positivegrowth37mesophilic
57765positivegrowth37mesophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 57765
  • oxygen tolerance: aerobe

spore formation

  • @ref: 29958
  • spore formation: no

halophily

  • @ref: 29958
  • salt: NaCl
  • growth: positive
  • tested relation: optimum
  • concentration: 5 %

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
2995828757fructose+carbon source
2995817234glucose+carbon source
2995829864mannitol+carbon source
2995837684mannose+carbon source
2995817268myo-inositol+carbon source
2995817814salicin+carbon source
2995827082trehalose+carbon source
2995818222xylose+carbon source
2995817632nitrate+reduction

enzymes

@refvalueactivityec
29958arylsulfatase+3.1.6.1
29958catalase+1.11.1.6
29958urease+3.5.1.5

API coryne

@refNITPYZPYRAPALbeta GURbeta GALalpha GLUbeta NAGESCUREGELGLURIBXYLMANMALLACSACGLYG
20093-+------++---------

API zym

@refAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
20093-++-+-+--++----+-+-

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentsampling date
11994scalp woundLA, New OrleansUSAUSANorth America
57765Human wound,scalpNew-Orleans,Charity HospitalUSAUSANorth America1987

isolation source categories

Cat1Cat2Cat3
#Host Body-Site#Other#Wound
#Host Body-Site#Organ#Skin, Nail, Hair

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
119942Risk group (German classification)
200932German classification

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Mycobacterium fortuitum strain ATCC 49404 16S ribosomal RNA gene, partial sequenceAF4805811470ena1766
20218Mycobacterium neworleansense strain ATCC 49404 16S ribosomal RNA gene, partial sequenceAY4570681483ena146018
20218M.fortuitum 16S rRNA (ATCC 49404)X65529885ena1766
11994Mycobacterium neworleansense strain W6705 16S ribosomal RNA gene, partial sequenceAY0125751480ena146018

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Mycolicibacterium neworleansense type strain: ATCC 49404GCA_001245615contigncbi146018
66792Mycobacterium neworleansense146018.3wgspatric146018

GC content

  • @ref: 29958
  • GC-content: 60

External links

@ref: 11994

culture collection no.: CCUG 47582, CIP 107827, CIP 105354, DSM 44679, ATCC 49404, CDC 87-1098

straininfo link

@refpassport
20218http://www.straininfo.net/strains/381466
20218http://www.straininfo.net/strains/121400
20218http://www.straininfo.net/strains/121402

Reference

@idauthorscataloguedoi/urltitlejournalpubmedID_cross_reference
11994Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 44679)https://www.dsmz.de/collection/catalogue/details/culture/DSM-44679
20093Wink, J.http://www.dsmz.de/microorganisms/wink_pdf/DSM44679.pdfCompendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig
20215D.Gleim, M.Kracht, N.Weiss et. al.http://www.dsmz.de/bacterial-diversity/prokaryotic-nomenclature-up-to-date.htmlProkaryotic Nomenclature Up-to-date - compilation of all names of Bacteria and Archaea, validly published according to the Bacteriological Code since 1. Jan. 1980, and validly published nomenclatural changes since
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
2632710.1099/ijs.0.02743-0
29958Barberan A, Caceres Velazquez H, Jones S, Fierer N.10.1128/mSphere.00237-17Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information26327
38235Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/5381
57765Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 47582)https://www.ccug.se/strain?id=47582
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)