Strain identifier

BacDive ID: 8519

Type strain: Yes

Species: Mycobacterium boenickei

Strain Designation: MF-657, W5998

Culture col. no.: DSM 44677, ATCC 49935, CCUG 47580, CIP 107829

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General

@ref: 11974

BacDive-ID: 8519

DSM-Number: 44677

keywords: aerobe, Bacteria, 16S sequence, genome sequence, mesophilic, gram-positive

description: Mycobacterium boenickei MF-657 is an aerobe, mesophilic, gram-positive bacterium that was isolated from leg wound.

strain history: <- J. M. Brown, CDC; W5998 <- R. J. Wallace <- Roche Biochemical Labs.

doi: 10.13145/bacdive8519.20201210.5

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinobacteria
  • domain: Bacteria
  • phylum: Actinobacteria
  • class: Actinobacteria
  • order: Mycobacteriales
  • family: Mycobacteriaceae
  • genus: Mycobacterium
  • species: Mycobacterium boenickei
  • full scientific name: Mycobacterium boenickei Schinsky et al. 2004
  • synonyms

    • @ref: 20215
    • synonym: Mycolicibacterium boenickei

@ref: 11974

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Mycobacteriaceae

genus: Mycobacterium

species: Mycobacterium boenickei

full scientific name: Mycobacterium boenickei Schinsky et al. 2004 emend. Nouioui et al. 2018

strain designation: MF-657, W5998

type strain: yes

Morphology

cell morphology

  • @ref: 29958
  • gram stain: positive

colony morphology

  • @ref: 57763
  • incubation period: 2 days

multimedia

  • @ref: 11974
  • multimedia content: https://www.dsmz.de/microorganisms/photos/DSM_44677.jpg
  • caption: Medium 645 37°C
  • license or copyright: (C) Leibniz-Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH
  • intellectual property rights: © Leibniz-Institut DSMZ

Culture and growth conditions

culture medium

@refnamegrowthlink
11974MIDDLEBROOK MEDIUM (DSMZ Medium 645)yeshttps://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium645.pdf
36377MEDIUM 55 - for Mycobacteriumyes

culture temp

@refgrowthtypetemperaturerange
11974positivegrowth37mesophilic
29958positivegrowth28-35mesophilic
29958positiveoptimum31.5mesophilic
36377positivegrowth37mesophilic
57763positivegrowth37mesophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 57763
  • oxygen tolerance: aerobe

spore formation

  • @ref: 29958
  • spore formation: no

halophily

  • @ref: 29958
  • salt: NaCl
  • growth: positive
  • tested relation: optimum
  • concentration: 5 %

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
2995828757fructose+carbon source
2995817234glucose+carbon source
2995829864mannitol+carbon source
2995837684mannose+carbon source
2995817268myo-inositol+carbon source
2995817814salicin+carbon source
2995827082trehalose+carbon source
2995817632nitrate+reduction

enzymes

@refvalueactivityec
29958arylsulfatase+3.1.6.1
29958catalase+1.11.1.6
29958urease+3.5.1.5

Isolation, sampling and environmental information

isolation

@refsample typecountryorigin.countrycontinent
11974leg woundUSAUSANorth America
57763Human wound,legUSAUSANorth America

isolation source categories

Cat1Cat2Cat3
#Host Body-Site#Other#Wound
#Host Body-Site#Limb#Leg

Safety information

risk assessment

  • @ref: 11974
  • biosafety level: 2
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

  • @ref: 11974
  • description: Mycobacterium bonickei strain W5998 16S ribosomal RNA gene, partial sequence
  • accession: AY012573
  • length: 1498
  • database: ena
  • NCBI tax ID: 146017

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Mycolicibacterium boenickei JCM 15653GCA_010731295completencbi146017
66792Mycolicibacterium boenickei CIP107829GCA_900166915contigncbi146017
66792Mycolicibacterium boenickei CCUG47580GCA_002553535contigncbi146017
66792Mycobacterium boenickei strain CCUG47580146017.6wgspatric146017
66792Mycobacterium boenickei strain CIP107829146017.5wgspatric146017

GC content

  • @ref: 29958
  • GC-content: 64

External links

@ref: 11974

culture collection no.: DSM 44677, ATCC 49935, CCUG 47580, CIP 107829

straininfo link

@refpassport
20218http://www.straininfo.net/strains/381456
20218http://www.straininfo.net/strains/121516

Reference

@idauthorscataloguedoi/urltitlejournalpubmedID_cross_reference
11974Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 44677)https://www.dsmz.de/collection/catalogue/details/culture/DSM-44677
20215D.Gleim, M.Kracht, N.Weiss et. al.http://www.dsmz.de/bacterial-diversity/prokaryotic-nomenclature-up-to-date.htmlProkaryotic Nomenclature Up-to-date - compilation of all names of Bacteria and Archaea, validly published according to the Bacteriological Code since 1. Jan. 1980, and validly published nomenclatural changes since
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
2632710.1099/ijs.0.02743-0
29958Barberan A, Caceres Velazquez H, Jones S, Fierer N.10.1128/mSphere.00237-17Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information26327
36377Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/5383
57763Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 47580)https://www.ccug.se/strain?id=47580
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)