Strain identifier

BacDive ID: 8511

Type strain: Yes

Species: Mycobacterium triplex

Strain history: CIP <- 1999, W.R. Butler, CDC, Atlanta, GA, USA: CDC 90-1019

NCBI tax ID(s): 47839 (species)

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General

@ref: 11938

BacDive-ID: 8511

DSM-Number: 44626

keywords: genome sequence, 16S sequence, Bacteria, aerobe, mesophilic, rod-shaped

description: Mycobacterium triplex CCUG 58693 is an aerobe, mesophilic, rod-shaped bacterium that was isolated from lymph nodes.

NCBI tax id

  • NCBI tax id: 47839
  • Matching level: species

strain history

@refhistory
11938<- CIP <- W. R. Butler, CDC
67770CIP 106108 <-- W. R. Butler CDC 90-1019.
123584CIP <- 1999, W.R. Butler, CDC, Atlanta, GA, USA: CDC 90-1019

doi: 10.13145/bacdive8511.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Mycobacteriales
  • family: Mycobacteriaceae
  • genus: Mycobacterium
  • species: Mycobacterium triplex
  • full scientific name: Mycobacterium triplex Floyd et al. 1997

@ref: 11938

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Mycobacteriaceae

genus: Mycobacterium

species: Mycobacterium triplex

full scientific name: Mycobacterium triplex Floyd et al. 1997 emend. Nouioui et al. 2018

type strain: yes

Morphology

cell morphology

  • @ref: 123584
  • cell shape: rod-shaped
  • motility: no

colony morphology

  • @ref: 61741
  • incubation period: 10-14 days

multimedia

  • @ref: 11938
  • multimedia content: https://www.dsmz.de/microorganisms/photos/DSM_44626.jpg
  • caption: Medium 645 37°C
  • intellectual property rights: © Leibniz-Institut DSMZ

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
11938MIDDLEBROOK MEDIUM (DSMZ Medium 645)yeshttps://mediadive.dsmz.de/medium/645Name: MIDDLEBROOK MEDIUM (DSMZ Medium 645) Composition: Bacto Middlebrook 7H10 agar 20.9945 g/l Glycerol Distilled water
123584CIP Medium 55yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=55

culture temp

@refgrowthtypetemperaturerange
11938positivegrowth37mesophilic
61741positivegrowth37mesophilic
67770positivegrowth37mesophilic
123584positivegrowth22-37
123584nogrowth10psychrophilic
123584nogrowth45thermophilic

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
61741aerobe
123584obligate aerobe

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
123584606565hippurate-hydrolysis
12358417632nitrate+reduction
12358416301nitrite-reduction
12358417632nitrate-respiration

metabolite production

  • @ref: 123584
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

enzymes

@refvalueactivityec
123584oxidase-
123584beta-galactosidase-3.2.1.23
123584gelatinase-
123584catalase+1.11.1.6
123584gamma-glutamyltransferase-2.3.2.2
123584urease+3.5.1.5
68382alkaline phosphatase+3.1.3.1
68382esterase (C 4)-
68382esterase lipase (C 8)+
68382lipase (C 14)-
68382leucine arylamidase+3.4.11.1
68382valine arylamidase-
68382cystine arylamidase-3.4.11.3
68382trypsin-3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase-3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase+3.2.1.20
68382beta-glucosidase-3.2.1.21
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
123584-+-+-+----++---+----

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentsampling date
11938lymph nodesCaliforniaUSAUSANorth America
61741Human lymf nodesCaliforniaUSAUSANorth America1990
67770Lymph nodeCAUSAUSANorth America
123584Lymph nodesCaliforniaUnited States of AmericaUSANorth America

isolation source categories

  • Cat1: #Host Body-Site
  • Cat2: #Organ
  • Cat3: #Lymph node

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
119382Risk group (German classification)
1235841Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Mycobacterium triplex strain 90-1019 16S ribosomal RNA gene, complete sequenceU576321474ena47839
20218Mycobacterium triplex strain CIP 106108 16S ribosomal RNA gene, partial sequenceAF547973542ena47839
11938Mycobacterium triplex strain ATCC 700071 16S ribosomal RNA gene, partial sequenceGQ1532791483ena47839

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Mycobacterium triplex strain DSM 4462647839.7wgspatric47839
66792Mycobacterium triplex strain DSM 4462647839.3wgspatric47839
66792Mycobacterium triplex DSM 446262619619252draftimg47839
67770Mycobacterium triplex DSM 44626GCA_000689255scaffoldncbi47839
67770Mycobacterium triplex DSM 44626GCA_002102415contigncbi47839

GC content

  • @ref: 67770
  • GC-content: 66.57
  • method: genome sequence analysis

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
gram-positiveyes89.659no
anaerobicno99.342no
halophileno95.918no
spore-formingno82.395no
glucose-utilyes72.42no
aerobicyes93.039yes
flagellatedno98.824no
motileno94.017no
thermophileno97.425yes
glucose-fermentno92.423no

External links

@ref: 11938

culture collection no.: CCUG 58693, DSMZ 44626, ATCC 70071, CDC 90-1019, CIP 106108, DSM 44626, ATCC 700071, JCM 14744

straininfo link

  • @ref: 77901
  • straininfo: 46211

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny8940431Characterization of an SAV organism and proposal of Mycobacterium triplex sp. nov.Floyd MM, Guthertz LS, Silcox VA, Duffey PS, Jang Y, Desmond EP, Crawford JT, Butler WRJ Clin Microbiol10.1128/jcm.34.12.2963-2967.19961996Bacterial Typing Techniques, Base Sequence, DNA Primers/genetics, Humans, Molecular Sequence Data, Mycobacterium/*classification/genetics/*isolation & purification, Mycobacterium Infections/epidemiology/microbiology, Mycobacterium avium Complex/classification, Phylogeny, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/genetics, Sequence Homology, Nucleic Acid, Species Specificity, Terminology as Topic, United States/epidemiologyPathogenicity
Genetics24874681Draft Genome Sequence of Mycobacterium triplex DSM 44626.Sassi M, Croce O, Robert C, Raoult D, Drancourt MGenome Announc10.1128/genomeA.00499-142014

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
11938Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 44626)https://www.dsmz.de/collection/catalogue/details/culture/DSM-44626
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
61741Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 58693)https://www.ccug.se/strain?id=58693
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68382Automatically annotated from API zym
77901Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID46211.1StrainInfo: A central database for resolving microbial strain identifiers
123584Curators of the CIPCollection of Institut Pasteur (CIP 106108)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20106108