Strain identifier

BacDive ID: 8499

Type strain: Yes

Species: Mycobacterium frederiksbergense

Strain Designation: FAn9

Variant: Isotype of BacDive ID 8504

Strain history: <- U. Karlson, Miljo and Energi, Roskilde Denmark, FAn9

NCBI tax ID(s): 117567 (species)

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General

@ref: 11692

BacDive-ID: 8499

DSM-Number: 44346

keywords: 16S sequence, Bacteria, mesophilic

description: Mycobacterium frederiksbergense FAn9 is a mesophilic bacterium that was isolated from coal tar contaminated soil former gas works site.

NCBI tax id

  • NCBI tax id: 117567
  • Matching level: species

strain history

  • @ref: 11692
  • history: <- U. Karlson, Miljo and Energi, Roskilde Denmark, FAn9

doi: 10.13145/bacdive8499.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Mycobacteriales
  • family: Mycobacteriaceae
  • genus: Mycobacterium
  • species: Mycobacterium frederiksbergense
  • full scientific name: Mycobacterium frederiksbergense Willumsen et al. 2001

  • synonyms

    • @ref: 20215
    • synonym: Mycolicibacterium frederiksbergense

@ref: 11692

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Mycobacteriaceae

genus: Mycobacterium

species: Mycobacterium frederiksbergense

full scientific name: Mycobacterium frederiksbergense Willumsen et al. 2001

strain designation: FAn9

variant: Isotype of BacDive ID 8504

type strain: yes

Morphology

colony morphology

  • @ref: 11692
  • incubation period: 8-14 days

multimedia

  • @ref: 11692
  • multimedia content: https://www.dsmz.de/microorganisms/photos/DSM_44346.jpg
  • caption: Medium 645 28°C
  • intellectual property rights: © Leibniz-Institut DSMZ

Culture and growth conditions

culture medium

  • @ref: 11692
  • name: MIDDLEBROOK MEDIUM (DSMZ Medium 645)
  • growth: yes
  • link: https://mediadive.dsmz.de/medium/645
  • composition: Name: MIDDLEBROOK MEDIUM (DSMZ Medium 645) Composition: Bacto Middlebrook 7H10 agar 20.9945 g/l Glycerol Distilled water

culture temp

  • @ref: 11692
  • growth: positive
  • type: growth
  • temperature: 28-37
  • range: mesophilic

Physiology and metabolism

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6836925115malate+assimilation
6836917128adipate-assimilation
6836927689decanoate-assimilation
6836924265gluconate+assimilation
6836917306maltose-assimilation
6836959640N-acetylglucosamine-assimilation
6836916899D-mannitol+assimilation
6836916024D-mannose-assimilation
683695291gelatin-hydrolysis
683694853esculin-hydrolysis
6836916199urea+hydrolysis
6836929016arginine-hydrolysis
6836917634D-glucose-fermentation
6836927897tryptophan-energy source
6836917632nitrate+reduction

metabolite production

  • @ref: 68369
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

metabolite tests

  • @ref: 68369
  • Chebi-ID: 35581
  • metabolite: indole
  • indole test: -

enzymes

@refvalueactivityec
68369gelatinase-
68369beta-glucosidase-3.2.1.21
68369urease+3.5.1.5
68369arginine dihydrolase-3.5.3.6
68382alkaline phosphatase-3.1.3.1
68382esterase (C 4)-
68382lipase (C 14)-
68382valine arylamidase-
68382cystine arylamidase-3.4.11.3
68382trypsin-3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase-3.1.3.2
68382naphthol-AS-BI-phosphohydrolase-
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase-3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase-3.2.1.20
68382beta-glucosidase-3.2.1.21
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
11692---+/--+/---------------

API 20NE

@refNO3TRPGLU_ FermADH ArgUREESCGELPNPGGLU_ AssimARAMNEMANNAGMALGNTCAPADIMLTCITPAC
11692+---+---+/-+/--+--+--+--

Isolation, sampling and environmental information

isolation

  • @ref: 11692
  • sample type: coal tar contaminated soil former gas works site
  • geographic location: Kopenhagen
  • country: Denmark
  • origin.country: DNK
  • continent: Europe

isolation source categories

Cat1Cat2Cat3
#Engineered#Contamination
#Environmental#Terrestrial#Geologic
#Environmental#Terrestrial#Soil

Safety information

risk assessment

  • @ref: 11692
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Mycobacterium frederiksbergense strain DSM 44346 16S ribosomal RNA gene, partial sequenceAF547925540ena117567
11692Mycobacterium frederiksbergense partial 16S rRNA gene, strain DSM 44346AJ2762741474ena117567

External links

@ref: 11692

culture collection no.: DSM 44346, KCTC 19100, NRRL B-24126

straininfo link

  • @ref: 77892
  • straininfo: 50584

literature

  • topic: Phylogeny
  • Pubmed-ID: 11594601
  • title: Mycobacterium frederiksbergense sp. nov., a novel polycyclic aromatic hydrocarbon-degrading Mycobacterium species.
  • authors: Willumsen P, Karlson U, Stackebrandt E, Kroppenstedt RM
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/00207713-51-5-1715
  • year: 2001
  • mesh: Biodegradation, Environmental, Coal Tar, DNA, Bacterial/analysis/genetics, DNA, Ribosomal/analysis/genetics, Denmark, Fatty Acids/analysis, Molecular Sequence Data, Mycobacterium/chemistry/*classification/metabolism, Mycolic Acids/analysis, Polycyclic Aromatic Hydrocarbons/*metabolism, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, *Soil Microbiology, *Soil Pollutants
  • topic2: Genetics

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
11692Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 44346)https://www.dsmz.de/collection/catalogue/details/culture/DSM-44346
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
68369Automatically annotated from API 20NE
68382Automatically annotated from API zym
77892Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID50584.1StrainInfo: A central database for resolving microbial strain identifiers