Strain identifier

BacDive ID: 8490

Type strain: Yes

Species: Mycobacterium heckeshornense

Strain Designation: S369

Strain history: CIP <- 2002, DSMZ <- A. Roth, Zehlendorf-Heckeshorn Hosp., Germany: strain S369

NCBI tax ID(s): 110505 (species)

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General

@ref: 11548

BacDive-ID: 8490

DSM-Number: 44428

keywords: genome sequence, 16S sequence, Bacteria, microaerophile, mesophilic, rod-shaped

description: Mycobacterium heckeshornense S369 is a microaerophile, mesophilic, rod-shaped bacterium that was isolated from respiratory tract women .

NCBI tax id

  • NCBI tax id: 110505
  • Matching level: species

strain history

@refhistory
11548<- A. Roth, Hospital Zehlendorf-Heckeshorn; S369
67770CIP 107347 <-- DSM 44428 <-- A. Roth S369.
119854CIP <- 2002, DSMZ <- A. Roth, Zehlendorf-Heckeshorn Hosp., Germany: strain S369

doi: 10.13145/bacdive8490.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Mycobacteriales
  • family: Mycobacteriaceae
  • genus: Mycobacterium
  • species: Mycobacterium heckeshornense
  • full scientific name: Mycobacterium heckeshornense Roth et al. 2001

@ref: 11548

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Mycobacteriaceae

genus: Mycobacterium

species: Mycobacterium heckeshornense

full scientific name: Mycobacterium heckeshornense Roth et al. 2001

strain designation: S369

type strain: yes

Morphology

cell morphology

  • @ref: 119854
  • cell shape: rod-shaped
  • motility: no

multimedia

  • @ref: 11548
  • multimedia content: https://www.dsmz.de/microorganisms/photos/DSM_44428.jpg
  • caption: Medium 645 37°C
  • intellectual property rights: © Leibniz-Institut DSMZ

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
11548MIDDLEBROOK MEDIUM (DSMZ Medium 645)yeshttps://mediadive.dsmz.de/medium/645Name: MIDDLEBROOK MEDIUM (DSMZ Medium 645) Composition: Bacto Middlebrook 7H10 agar 20.9945 g/l Glycerol Distilled water
36334MEDIUM 93 - for Mycobacterium avium subsp. sylvaticumyesDistilled water make up to (900.000 ml);Glycerol (4.500 ml);Middlebrook7H10 agar (17.000 g);Middelbrook OADC enrichment (100.000 ml)
119854CIP Medium 93yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=93

culture temp

@refgrowthtypetemperaturerange
11548positivegrowth37mesophilic
36334positivegrowth37mesophilic
59358positivegrowth37mesophilic
67770positivegrowth37mesophilic
119854positivegrowth45thermophilic
119854nogrowth10psychrophilic
119854nogrowth22psychrophilic
119854nogrowth30mesophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 59358
  • oxygen tolerance: microaerophile

observation

  • @ref: 67770
  • observation: quinones: MK-9(H2)

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
119854606565hippurate-hydrolysis
11985417632nitrate-reduction
11985416301nitrite-reduction

metabolite production

  • @ref: 119854
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

enzymes

@refvalueactivityec
119854oxidase-
119854beta-galactosidase+3.2.1.23
119854gelatinase-
119854catalase-1.11.1.6
119854gamma-glutamyltransferase+2.3.2.2
119854urease-3.5.1.5
68382alkaline phosphatase-3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)+
68382lipase (C 14)-
68382leucine arylamidase+3.4.11.1
68382valine arylamidase-
68382cystine arylamidase-3.4.11.3
68382trypsin-3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase-3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase-3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase+3.2.1.20
68382beta-glucosidase-3.2.1.21
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
119854--++-+-----+---+----

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentisolation date
11548respiratory tract women (immunocompetent)BerlinGermanyDEUEurope
59358Human respiratory tract,woman,immunocompetentGermanyDEUEurope
67770Respiratory tract of an immunocompetent womenBerlinGermanyDEUEurope
119854Human, Respiratory tractBerlinGermanyDEUEurope1994

isolation source categories

Cat1Cat2Cat3
#Host#Human#Female
#Host Body-Site#Oral cavity and airways#Airways

taxonmaps

  • @ref: 69479
  • File name: preview.99_784.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_10;96_475;97_543;98_626;99_784&stattab=map
  • Last taxonomy: Mycobacterium heckeshornense
  • 16S sequence: AF174290
  • Sequence Identity:
  • Total samples: 199
  • soil counts: 115
  • aquatic counts: 11
  • animal counts: 2
  • plant counts: 71

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
115482Risk group (German classification)
1198541Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Mycobacterium heckeshornense strain DSM 44428 16S ribosomal RNA gene, partial sequenceAF547934560ena110505
11548Mycobacterium heckeshornense 16S ribosomal RNA gene, partial sequenceAF1742901527ena110505

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Mycobacterium heckeshornense JCM 15655GCA_016592155completencbi110505
66792Mycobacterium heckeshornense strain JCM 15655110505.9completepatric110505
66792Mycobacterium heckeshornense DSM 44428GCA_025822025scaffoldncbi110505

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
flagellatedno99.274no
gram-positiveyes86.935no
anaerobicno98.32no
aerobicyes89.838no
halophileno93.802no
spore-formingno84.976no
thermophileno86.942no
glucose-utilyes68.923no
motileno96.774no
glucose-fermentno92.377no

External links

@ref: 11548

culture collection no.: DSM 44428, CCUG 51897, JCM 15655, CIP 107347

straininfo link

  • @ref: 77884
  • straininfo: 50628

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny11060075Mycobacterium heckeshornense sp. nov., A new pathogenic slowly growing Mycobacterium sp. Causing cavitary lung disease in an immunocompetent patient.Roth A, Reischl U, Schonfeld N, Naumann L, Emler S, Fischer M, Mauch H, Loddenkemper R, Kroppenstedt RMJ Clin Microbiol10.1128/JCM.38.11.4102-4107.20002000Adult, Base Sequence, DNA, Bacterial/analysis/genetics, DNA, Ribosomal/analysis/genetics, Female, Humans, Immunocompetence, Molecular Sequence Data, Mycobacterium Infections, Nontuberculous/*microbiology, Nontuberculous Mycobacteria/chemistry/*classification/genetics/*isolation & purification/pathogenicity, Phylogeny, RNA, Ribosomal, 16S/genetics, Tuberculosis, Pulmonary/*microbiology, VirulencePathogenicity
Genetics33707319Complete Genome Sequence of Mycobacterium heckeshornense JCM 15655(T), Closely Related to a Pathogenic Nontuberculous Mycobacterial Species, Mycobacterium xenopi.Yoshida M, Fukano H, Asakura T, Suzuki M, Hoshino YMicrobiol Resour Announc10.1128/MRA.00020-212021

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
11548Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 44428)https://www.dsmz.de/collection/catalogue/details/culture/DSM-44428
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
36334Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/4773
59358Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 51897)https://www.ccug.se/strain?id=51897
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
77884Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID50628.1StrainInfo: A central database for resolving microbial strain identifiers
119854Curators of the CIPCollection of Institut Pasteur (CIP 107347)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20107347