Strain identifier
BacDive ID: 8483
Type strain:
Species: Mycobacterium monacense
Strain Designation: B9-21-178, T 15752
Strain history: CIP <- 2006, DSMZ <- L. Naumann, Rejensbury Univ. <- Th. Miethke, Munich Univ., Munich, Germany
NCBI tax ID(s): 1226751 (strain), 85693 (species)
General
@ref: 11719
BacDive-ID: 8483
DSM-Number: 44395
keywords: genome sequence, 16S sequence, Bacteria, obligate aerobe, mesophilic
description: Mycobacterium monacense B9-21-178 is an obligate aerobe, mesophilic bacterium that was isolated from bronchial lavage sample.
NCBI tax id
NCBI tax id | Matching level |
---|---|
1226751 | strain |
85693 | species |
strain history
@ref | history |
---|---|
11719 | <- L. Naumann, Univ. Regensburg <- Th. Miethke, Techn. Univ. München; T 15752 |
67770 | CIP 109237 <-- DSM 44395 <-- L. Naumann B9-21-178 <-- Th. Miethke. |
121007 | CIP <- 2006, DSMZ <- L. Naumann, Rejensbury Univ. <- Th. Miethke, Munich Univ., Munich, Germany |
doi: 10.13145/bacdive8483.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/actinomycetota
- domain: Bacteria
- phylum: Actinomycetota
- class: Actinomycetes
- order: Mycobacteriales
- family: Mycobacteriaceae
- genus: Mycobacterium
- species: Mycobacterium monacense
- full scientific name: Mycobacterium monacense Reischl et al. 2006
synonyms
- @ref: 20215
- synonym: Mycolicibacterium monacense
@ref: 11719
domain: Bacteria
phylum: Actinobacteria
class: Actinobacteria
order: Actinomycetales
family: Mycobacteriaceae
genus: Mycobacterium
species: Mycobacterium monacense
full scientific name: Mycobacterium monacense Reischl et al. 2006 emend. Nouioui et al. 2018
strain designation: B9-21-178, T 15752
type strain: yes
Morphology
cell morphology
@ref | motility | confidence | gram stain |
---|---|---|---|
69480 | no | 92.526 | |
69480 | 100 | positive | |
121007 | yes |
colony morphology
@ref | colony color | incubation period | medium used |
---|---|---|---|
19861 | Maize yellow | 10-14 days | ISP 2 |
19861 | Maize yellow | 10-14 days | ISP 3 |
19861 | Maize yellow | 10-14 days | ISP 4 |
19861 | Maize yellow | 10-14 days | ISP 5 |
19861 | Maize yellow | 10-14 days | ISP 6 |
19861 | Maize yellow | 10-14 days | ISP 7 |
multicellular morphology
@ref | forms multicellular complex | medium name |
---|---|---|
19861 | no | ISP 2 |
19861 | no | ISP 3 |
19861 | no | ISP 4 |
19861 | no | ISP 5 |
19861 | no | ISP 6 |
19861 | no | ISP 7 |
multimedia
- @ref: 11719
- multimedia content: https://www.dsmz.de/microorganisms/photos/DSM_44395.jpg
- caption: Medium 219 37°C
- intellectual property rights: © Leibniz-Institut DSMZ
Culture and growth conditions
culture medium
@ref | name | growth | link | composition |
---|---|---|---|---|
11719 | MYCOBACTERIUM MEDIUM (DSMZ Medium 219) | yes | https://mediadive.dsmz.de/medium/219 | Name: MYCOBACTERIUM MEDIUM (DSMZ Medium 219) Composition: Agar 20.0 g/l Na2HPO4 x 12 H2O 2.5 g/l Yeast extract 2.0 g/l Proteose peptone no. 3 2.0 g/l Casein peptone 2.0 g/l Sodium citrate 1.5 g/l KH2PO4 1.0 g/l MgSO4 x 7 H2O 0.6 g/l Tween 0.5 g/l Glycerol Distilled water |
19861 | ISP 2 | yes | Name: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes | |
19861 | ISP 3 | yes | Name: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms: | |
19861 | ISP 4 | yes | Name: ISP 4; DSM 547 Solution I: Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml. Solution II: CaCO3 2.0 g K2HPO4 (anhydrous) 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g (NH4)2SO4 2.0 g Distilled water 500.0 ml Trace salt solution (see below) 1.0 ml The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min. Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml | |
19861 | ISP 5 | yes | Name: ISP 5 (5323) Composition L-Asparagine 1.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l Salt solution (see preparation) 1.0 ml/l Agar 20.0 g/l Preparation: Salt solution 1.0 g FeSO4 x 7 H2O 1.0 g MnCl2 x 4 H2O 1.0 g ZNSO4 x 7 H2O in 100 ml water Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.2 Usage: Maintenance and taxonomy Organisms: All Actinomycetes | |
19861 | ISP 6 | yes | Name: ISP 6 (5318) Composition Peptone 15.0 g/l Proteose peptose 5.0 g/l Ferric ammonium citrate 0.5 g/l Sodium glycerophosphate 1.0 g/l Sodium thiosulfate 0.08 g/l Yeast extract 1.0 g/l Agar 15.0 g/l Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Production of melanoid pigments Organisms: All Actinomycetes | |
19861 | ISP 7 | yes | Name: ISP 7 (5322) Composition Glycerol 15.0 g/l L-Tyrosine 0.5 g/l L-Asparagine 1.0 g/l K2HPO4 0.5 g/l NaCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Trace element solution 5343 1.0 ml/l Agar 20.0 Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.3 Usage: Production of melanoid pigments Organisms: All Actinomycetes | |
36393 | MEDIUM 55 - for Mycobacterium | yes | ||
121007 | CIP Medium 55 | yes | https://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=55 |
culture temp
@ref | growth | type | temperature | range |
---|---|---|---|---|
19861 | positive | optimum | 37 | mesophilic |
11719 | positive | growth | 37 | mesophilic |
36393 | positive | growth | 37 | mesophilic |
67770 | positive | growth | 37 | mesophilic |
Physiology and metabolism
oxygen tolerance
- @ref: 121007
- oxygen tolerance: obligate aerobe
spore formation
- @ref: 69480
- spore formation: no
- confidence: 99.901
metabolite utilization
@ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
---|---|---|---|---|
121007 | 17632 | nitrate | + | reduction |
121007 | 16301 | nitrite | - | reduction |
68379 | 17632 | nitrate | - | reduction |
68379 | 4853 | esculin | - | hydrolysis |
68379 | 16199 | urea | + | hydrolysis |
68379 | 5291 | gelatin | - | hydrolysis |
68379 | 17634 | D-glucose | - | fermentation |
68379 | 16988 | D-ribose | - | fermentation |
68379 | 65327 | D-xylose | - | fermentation |
68379 | 16899 | D-mannitol | - | fermentation |
68379 | 17306 | maltose | - | fermentation |
68379 | 17716 | lactose | - | fermentation |
68379 | 17992 | sucrose | + | fermentation |
68379 | 28087 | glycogen | - | fermentation |
metabolite production
- @ref: 121007
- Chebi-ID: 35581
- metabolite: indole
- production: no
enzymes
@ref | value | activity | ec |
---|---|---|---|
68379 | gelatinase | - | |
68379 | urease | + | 3.5.1.5 |
68379 | beta-glucosidase | - | 3.2.1.21 |
68379 | N-acetyl-beta-glucosaminidase | - | 3.2.1.52 |
68379 | alpha-glucosidase | + | 3.2.1.20 |
68379 | beta-galactosidase | - | 3.2.1.23 |
68379 | beta-glucuronidase | - | 3.2.1.31 |
68379 | alkaline phosphatase | - | 3.1.3.1 |
68379 | pyrrolidonyl arylamidase | - | 3.4.19.3 |
68379 | pyrazinamidase | + | 3.5.1.B15 |
121007 | oxidase | - | |
121007 | catalase | + | 1.11.1.6 |
121007 | urease | + | 3.5.1.5 |
68382 | esterase (C 4) | + | |
68382 | esterase lipase (C 8) | + | |
68382 | leucine arylamidase | + | 3.4.11.1 |
68382 | valine arylamidase | + | |
68382 | cystine arylamidase | + | 3.4.11.3 |
68382 | trypsin | + | 3.4.21.4 |
68382 | alpha-chymotrypsin | - | 3.4.21.1 |
68382 | acid phosphatase | + | 3.1.3.2 |
68382 | naphthol-AS-BI-phosphohydrolase | + | |
68382 | alpha-galactosidase | - | 3.2.1.22 |
68382 | beta-galactosidase | - | 3.2.1.23 |
68382 | alpha-glucosidase | + | 3.2.1.20 |
68382 | beta-glucosidase | - | 3.2.1.21 |
68382 | N-acetyl-beta-glucosaminidase | - | 3.2.1.52 |
68382 | alpha-mannosidase | - | 3.2.1.24 |
68382 | alpha-fucosidase | - | 3.2.1.51 |
API coryne
@ref | NIT | PYZ | PYRA | PAL | beta GUR | beta GAL | alpha GLU | beta NAG | ESC | URE | GEL | GLU | RIB | XYL | MAN | MAL | LAC | SAC | GLYG |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
19861 | - | + | - | - | - | - | + | - | - | + | - | - | - | - | - | - | - | + | - |
API zym
@ref | Alkaline phosphatase | Esterase | Esterase Lipase | Lipase | Leucine arylamidase | Valine arylamidase | Cystine arylamidase | Trypsin | alpha- Chymotrypsin | Acid phosphatase | Naphthol-AS-BI-phosphohydrolase | alpha- Galactosidase | beta- Galactosidase | beta- Glucuronidase | alpha- Glucosidase | beta- Glucosidase | N-acetyl-beta- glucosaminidase | alpha- Mannosidase | alpha- Fucosidase | Control |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
19861 | - | + | + | - | + | + | + | + | - | + | + | - | - | - | + | - | - | - | - | |
121007 | + | + | + | + | + | + | + | + | - | + | + | - | - | + | + | - | - | - | - | - |
Isolation, sampling and environmental information
isolation
@ref | sample type | geographic location | country | origin.country | continent | isolation date |
---|---|---|---|---|---|---|
11719 | bronchial lavage sample | Bavaria | Germany | DEU | Europe | |
67770 | Bronchial lavage sample | Germany | DEU | Europe | ||
121007 | Human, Wound infection | München | Germany | DEU | Europe | 1998 |
isolation source categories
Cat1 | Cat2 | Cat3 |
---|---|---|
#Infection | #Patient | #Specimen |
#Host Body-Site | #Oral cavity and airways | |
#Host Body Product | #Oral cavity and Airways | #Bronchial wash |
Safety information
risk assessment
@ref | biosafety level | biosafety level comment |
---|---|---|
11719 | 2 | Risk group (German classification) |
19861 | 2 | Risk group |
121007 | 1 | Risk group (French classification) |
Sequence information
16S sequences
@ref | description | accession | length | database | NCBI tax ID |
---|---|---|---|---|---|
20218 | Mycobacterium monacense strain DSM 44395 16S ribosomal RNA gene, partial sequence | EU191914 | 459 | ena | 1226751 |
11719 | Mycobacterium monacense strain B9-21-178 16S ribosomal RNA gene, partial sequence | AF107039 | 1473 | ena | 85693 |
Genome sequences
@ref | description | accession | assembly level | database | NCBI tax ID |
---|---|---|---|---|---|
66792 | Mycolicibacterium monacense JCM 15658 | GCA_010731575 | complete | ncbi | 85693 |
66792 | Mycolicibacterium monacense DSM 44395 | GCA_009931355 | complete | ncbi | 1226751 |
66792 | Mycobacterium monacense DSM 44395 strain DSM 44395 | 1226751.3 | wgs | patric | 1226751 |
66792 | Mycolicibacterium monacense DSM 44395 | 1226751.7 | complete | patric | 1226751 |
66792 | Mycolicibacterium monacense strain JCM 15658 | 85693.8 | complete | patric | 85693 |
67770 | Mycolicibacterium monacense DSM 44395 | GCA_002086655 | contig | ncbi | 1226751 |
66792 | Mycolicibacterium monacense DSM 44395 | GCA_027886265 | contig | ncbi | 1226751 |
GC content
- @ref: 67770
- GC-content: 68.4
- method: genome sequence analysis
Genome-based predictions
predictions
trait | prediction | confidence | training_data |
---|---|---|---|
spore-forming | no | 86 | no |
motile | no | 91.905 | no |
flagellated | no | 98.783 | no |
gram-positive | yes | 90.613 | no |
anaerobic | no | 99.233 | no |
aerobic | yes | 90.41 | no |
halophile | no | 89.035 | no |
spore-forming | no | 85.894 | no |
glucose-util | yes | 87.703 | no |
thermophile | no | 99.292 | yes |
glucose-ferment | no | 90.968 | no |
External links
@ref: 11719
culture collection no.: DSM 44395, CIP 109237, JCM 15658
straininfo link
- @ref: 77877
- straininfo: 297260
literature
topic | Pubmed-ID | title | authors | journal | DOI | year | mesh | topic2 |
---|---|---|---|---|---|---|---|---|
Phylogeny | 17082393 | Mycobacterium monacense sp. nov. | Reischl U, Melzl H, Kroppenstedt RM, Miethke T, Naumann L, Mariottini A, Mazzarelli G, Tortoli E | Int J Syst Evol Microbiol | 10.1099/ijs.0.64527-0 | 2006 | Adult, Aged, 80 and over, Anti-Bacterial Agents/pharmacology, Bacterial Proteins/genetics, Bronchoalveolar Lavage Fluid/microbiology, Chaperonin 60, Chaperonins/genetics, Child, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, DNA, Ribosomal Spacer/chemistry/genetics, Female, Genes, rRNA, Germany, Humans, Italy, Male, Microbial Sensitivity Tests, Molecular Sequence Data, Mycobacterium Infections, Nontuberculous/*microbiology, Mycolic Acids/*analysis, Nontuberculous Mycobacteria/*classification/*isolation & purification/physiology, Phylogeny, Pigments, Biological, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Sequence Homology, Nucleic Acid, Sputum/microbiology, Wound Infection/microbiology | Pathogenicity |
Phylogeny | 21742818 | Mycobacterium litorale sp. nov., a rapidly growing mycobacterium from soil. | Zhang Y, Zhang J, Fang C, Pang H, Fan J | Int J Syst Evol Microbiol | 10.1099/ijs.0.033449-0 | 2011 | Bacterial Proteins/genetics, Bacterial Typing Techniques, Chaperonin 60/genetics, China, Cluster Analysis, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, DNA, Ribosomal Spacer/chemistry/genetics, Fatty Acids/analysis, Molecular Sequence Data, Mycobacterium/*classification/genetics/growth & development/*isolation & purification, Mycolic Acids/analysis, Nucleic Acid Hybridization, Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, *Soil Microbiology | Genetics |
Phylogeny | 34878372 | Mycolicibacterium baixiangningiae sp. nov. and Mycolicibacterium mengxianglii sp. nov., two new rapidly growing mycobacterial species. | Cheng Y, Lei W, Wang X, Tian Z, Liu H, Yang J, Lu S, Lai XH, Pu J, Huang Y, Zhang S, Yang C, Lian X, Bai Y, Wan K, Wang S, Xu J | Int J Syst Evol Microbiol | 10.1099/ijsem.0.005019 | 2021 | Animals, Antelopes/*microbiology, Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Feces/microbiology, Mycobacteriaceae/*classification/isolation & purification, Nucleic Acid Hybridization, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Tibet | Transcriptome |
Reference
@id | authors | catalogue | doi/url | title | journal | pubmed |
---|---|---|---|---|---|---|
11719 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 44395) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-44395 | |||
19861 | Wink, J. | http://www.dsmz.de/microorganisms/wink_pdf/DSM44395.pdf | Compendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig | |||
20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |||
20218 | Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P. | 10.1016/j.syapm.2013.11.002 | StrainInfo introduces electronic passports for microorganisms. | Syst Appl Microbiol. 37: 42-50 2014 | 24321274 | |
36393 | Curators of the CIP | https://brclims.pasteur.fr/brcWeb/souche/detail/1/6956 | ||||
66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |||
67770 | Curators of the JCM | https://jcm.brc.riken.jp/en/ | ||||
68379 | Automatically annotated from API Coryne | |||||
68382 | Automatically annotated from API zym | |||||
69480 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |||
77877 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID297260.1 | StrainInfo: A central database for resolving microbial strain identifiers | |||
121007 | Curators of the CIP | Collection of Institut Pasteur (CIP 109237) | https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20109237 |