Strain identifier

BacDive ID: 8483

Type strain: Yes

Species: Mycobacterium monacense

Strain Designation: B9-21-178, T 15752

Strain history: CIP <- 2006, DSMZ <- L. Naumann, Rejensbury Univ. <- Th. Miethke, Munich Univ., Munich, Germany

For citation purpose refer to the digital object identifier (doi) of the current version.
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General

@ref: 11719

BacDive-ID: 8483

DSM-Number: 44395

keywords: genome sequence, 16S sequence, Bacteria, obligate aerobe, mesophilic

description: Mycobacterium monacense B9-21-178 is an obligate aerobe, mesophilic bacterium that was isolated from bronchial lavage sample.

NCBI tax id

NCBI tax idMatching level
1226751strain
85693species

strain history

@refhistory
11719<- L. Naumann, Univ. Regensburg <- Th. Miethke, Techn. Univ. München; T 15752
67770CIP 109237 <-- DSM 44395 <-- L. Naumann B9-21-178 <-- Th. Miethke.
121007CIP <- 2006, DSMZ <- L. Naumann, Rejensbury Univ. <- Th. Miethke, Munich Univ., Munich, Germany

doi: 10.13145/bacdive8483.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Mycobacteriales
  • family: Mycobacteriaceae
  • genus: Mycobacterium
  • species: Mycobacterium monacense
  • full scientific name: Mycobacterium monacense Reischl et al. 2006
  • synonyms

    • @ref: 20215
    • synonym: Mycolicibacterium monacense

@ref: 11719

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Mycobacteriaceae

genus: Mycobacterium

species: Mycobacterium monacense

full scientific name: Mycobacterium monacense Reischl et al. 2006 emend. Nouioui et al. 2018

strain designation: B9-21-178, T 15752

type strain: yes

Morphology

cell morphology

@refmotilityconfidencegram stain
69480no92.526
69480100positive
121007yes

colony morphology

@refcolony colorincubation periodmedium used
19861Maize yellow10-14 daysISP 2
19861Maize yellow10-14 daysISP 3
19861Maize yellow10-14 daysISP 4
19861Maize yellow10-14 daysISP 5
19861Maize yellow10-14 daysISP 6
19861Maize yellow10-14 daysISP 7

multicellular morphology

@refforms multicellular complexmedium name
19861noISP 2
19861noISP 3
19861noISP 4
19861noISP 5
19861noISP 6
19861noISP 7

multimedia

  • @ref: 11719
  • multimedia content: https://www.dsmz.de/microorganisms/photos/DSM_44395.jpg
  • caption: Medium 219 37°C
  • intellectual property rights: © Leibniz-Institut DSMZ

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
11719MYCOBACTERIUM MEDIUM (DSMZ Medium 219)yeshttps://mediadive.dsmz.de/medium/219Name: MYCOBACTERIUM MEDIUM (DSMZ Medium 219) Composition: Agar 20.0 g/l Na2HPO4 x 12 H2O 2.5 g/l Yeast extract 2.0 g/l Proteose peptone no. 3 2.0 g/l Casein peptone 2.0 g/l Sodium citrate 1.5 g/l KH2PO4 1.0 g/l MgSO4 x 7 H2O 0.6 g/l Tween 0.5 g/l Glycerol Distilled water
19861ISP 2yesName: ISP 2 / Yeast Malt Agar (5265); 5265 Composition Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l Agar 15.0 g/l Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.0 Usage: Maintenance and Taxonomy Organisms: All Actinomycetes
19861ISP 3yesName: ISP 3; 5315 Composition Dog oat flakes 20.0 g/l Trace element solution (5314) 2.5 ml/l Agar 18.0 g/l Preparation: Oat flakes are cooked for 20 minutes, trace element solution and agar are added (in the case of non rolled oat flakes the suspension has to bee filtrated). Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.8 Usage: Maintenance and taxonomy (e.g. SEM As liquid medium for metabolite production) Organisms: All Actinomycetes Trace element solution 5314 Name: Trace element solution 5314; 5314 Composition CaCl2 x H2O 3.0 g/l Fe-III-citrate 1.0 g/l MnSO4 0.2 g/l ZnCl2 0.1 g/l CuSO4 x 5 H2O 0.025 g/l Sodium tetra borate 0.2 g/l CoCl2 x 6 H2O 0.004 g/l Sodium molybdate 0.01 g/l Preparation: Use double destillated water. Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Trace element solution for different media Organisms:
19861ISP 4yesName: ISP 4; DSM 547 Solution I: Difco soluble starch, 10.0 g. Make a paste of the starch with a small amount of cold distilled water and bring to a volume of 500 ml. Solution II: CaCO3 2.0 g K2HPO4 (anhydrous) 1.0 g MgSO4 x 7 H2O 1.0 g NaCl 1.0 g (NH4)2SO4 2.0 g Distilled water 500.0 ml Trace salt solution (see below) 1.0 ml The pH should be between 7.0 and 7.4. Do not adjust if it is within this range. Mix solutions I and II together. Add 20.0 g agar. Liquify agar by steaming at 100°C for 10 to 20 min. Trace element solution: FeSO4 x 7 H2O 0.1 g MnCl2 x 4 H2O 0.1 g ZnSO4 x 7 H2O 0.1 g Distilled water 100.0 ml
19861ISP 5yesName: ISP 5 (5323) Composition L-Asparagine 1.0 g/l Glycerol 10.0 g/l K2HPO4 1.0 g/l Salt solution (see preparation) 1.0 ml/l Agar 20.0 g/l Preparation: Salt solution 1.0 g FeSO4 x 7 H2O 1.0 g MnCl2 x 4 H2O 1.0 g ZNSO4 x 7 H2O in 100 ml water Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.2 Usage: Maintenance and taxonomy Organisms: All Actinomycetes
19861ISP 6yesName: ISP 6 (5318) Composition Peptone 15.0 g/l Proteose peptose 5.0 g/l Ferric ammonium citrate 0.5 g/l Sodium glycerophosphate 1.0 g/l Sodium thiosulfate 0.08 g/l Yeast extract 1.0 g/l Agar 15.0 g/l Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Production of melanoid pigments Organisms: All Actinomycetes
19861ISP 7yesName: ISP 7 (5322) Composition Glycerol 15.0 g/l L-Tyrosine 0.5 g/l L-Asparagine 1.0 g/l K2HPO4 0.5 g/l NaCl 0.5 g/l FeSO4 x 7 H2O 0.01 g/l Trace element solution 5343 1.0 ml/l Agar 20.0 Sterilisation: 20 minutes at 121°C pH before sterilisation: 7.3 Usage: Production of melanoid pigments Organisms: All Actinomycetes
36393MEDIUM 55 - for Mycobacteriumyes
121007CIP Medium 55yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=55

culture temp

@refgrowthtypetemperaturerange
19861positiveoptimum37mesophilic
11719positivegrowth37mesophilic
36393positivegrowth37mesophilic
67770positivegrowth37mesophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 121007
  • oxygen tolerance: obligate aerobe

spore formation

  • @ref: 69480
  • spore formation: no
  • confidence: 99.901

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
12100717632nitrate+reduction
12100716301nitrite-reduction
6837917632nitrate-reduction
683794853esculin-hydrolysis
6837916199urea+hydrolysis
683795291gelatin-hydrolysis
6837917634D-glucose-fermentation
6837916988D-ribose-fermentation
6837965327D-xylose-fermentation
6837916899D-mannitol-fermentation
6837917306maltose-fermentation
6837917716lactose-fermentation
6837917992sucrose+fermentation
6837928087glycogen-fermentation

metabolite production

  • @ref: 121007
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

enzymes

@refvalueactivityec
68379gelatinase-
68379urease+3.5.1.5
68379beta-glucosidase-3.2.1.21
68379N-acetyl-beta-glucosaminidase-3.2.1.52
68379alpha-glucosidase+3.2.1.20
68379beta-galactosidase-3.2.1.23
68379beta-glucuronidase-3.2.1.31
68379alkaline phosphatase-3.1.3.1
68379pyrrolidonyl arylamidase-3.4.19.3
68379pyrazinamidase+3.5.1.B15
121007oxidase-
121007catalase+1.11.1.6
121007urease+3.5.1.5
68382esterase (C 4)+
68382esterase lipase (C 8)+
68382leucine arylamidase+3.4.11.1
68382valine arylamidase+
68382cystine arylamidase+3.4.11.3
68382trypsin+3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase-3.2.1.23
68382alpha-glucosidase+3.2.1.20
68382beta-glucosidase-3.2.1.21
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API coryne

@refNITPYZPYRAPALbeta GURbeta GALalpha GLUbeta NAGESCUREGELGLURIBXYLMANMALLACSACGLYG
19861-+----+--+-------+-

API zym

@refAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- FucosidaseControl
19861-++-++++-++---+----
121007++++++++-++--++-----

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentisolation date
11719bronchial lavage sampleBavariaGermanyDEUEurope
67770Bronchial lavage sampleGermanyDEUEurope
121007Human, Wound infectionMünchenGermanyDEUEurope1998

isolation source categories

Cat1Cat2Cat3
#Infection#Patient#Specimen
#Host Body-Site#Oral cavity and airways
#Host Body Product#Oral cavity and Airways#Bronchial wash

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
117192Risk group (German classification)
198612Risk group
1210071Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Mycobacterium monacense strain DSM 44395 16S ribosomal RNA gene, partial sequenceEU191914459ena1226751
11719Mycobacterium monacense strain B9-21-178 16S ribosomal RNA gene, partial sequenceAF1070391473ena85693

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Mycolicibacterium monacense JCM 15658GCA_010731575completencbi85693
66792Mycolicibacterium monacense DSM 44395GCA_009931355completencbi1226751
66792Mycobacterium monacense DSM 44395 strain DSM 443951226751.3wgspatric1226751
66792Mycolicibacterium monacense DSM 443951226751.7completepatric1226751
66792Mycolicibacterium monacense strain JCM 1565885693.8completepatric85693
67770Mycolicibacterium monacense DSM 44395GCA_002086655contigncbi1226751
66792Mycolicibacterium monacense DSM 44395GCA_027886265contigncbi1226751

GC content

  • @ref: 67770
  • GC-content: 68.4
  • method: genome sequence analysis

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno86no
motileno91.905no
flagellatedno98.783no
gram-positiveyes90.613no
anaerobicno99.233no
aerobicyes90.41no
halophileno89.035no
spore-formingno85.894no
glucose-utilyes87.703no
thermophileno99.292yes
glucose-fermentno90.968no

External links

@ref: 11719

culture collection no.: DSM 44395, CIP 109237, JCM 15658

straininfo link

  • @ref: 77877
  • straininfo: 297260

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny17082393Mycobacterium monacense sp. nov.Reischl U, Melzl H, Kroppenstedt RM, Miethke T, Naumann L, Mariottini A, Mazzarelli G, Tortoli EInt J Syst Evol Microbiol10.1099/ijs.0.64527-02006Adult, Aged, 80 and over, Anti-Bacterial Agents/pharmacology, Bacterial Proteins/genetics, Bronchoalveolar Lavage Fluid/microbiology, Chaperonin 60, Chaperonins/genetics, Child, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, DNA, Ribosomal Spacer/chemistry/genetics, Female, Genes, rRNA, Germany, Humans, Italy, Male, Microbial Sensitivity Tests, Molecular Sequence Data, Mycobacterium Infections, Nontuberculous/*microbiology, Mycolic Acids/*analysis, Nontuberculous Mycobacteria/*classification/*isolation & purification/physiology, Phylogeny, Pigments, Biological, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Sequence Homology, Nucleic Acid, Sputum/microbiology, Wound Infection/microbiologyPathogenicity
Phylogeny21742818Mycobacterium litorale sp. nov., a rapidly growing mycobacterium from soil.Zhang Y, Zhang J, Fang C, Pang H, Fan JInt J Syst Evol Microbiol10.1099/ijs.0.033449-02011Bacterial Proteins/genetics, Bacterial Typing Techniques, Chaperonin 60/genetics, China, Cluster Analysis, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, DNA, Ribosomal Spacer/chemistry/genetics, Fatty Acids/analysis, Molecular Sequence Data, Mycobacterium/*classification/genetics/growth & development/*isolation & purification, Mycolic Acids/analysis, Nucleic Acid Hybridization, Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, *Soil MicrobiologyGenetics
Phylogeny34878372Mycolicibacterium baixiangningiae sp. nov. and Mycolicibacterium mengxianglii sp. nov., two new rapidly growing mycobacterial species.Cheng Y, Lei W, Wang X, Tian Z, Liu H, Yang J, Lu S, Lai XH, Pu J, Huang Y, Zhang S, Yang C, Lian X, Bai Y, Wan K, Wang S, Xu JInt J Syst Evol Microbiol10.1099/ijsem.0.0050192021Animals, Antelopes/*microbiology, Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Feces/microbiology, Mycobacteriaceae/*classification/isolation & purification, Nucleic Acid Hybridization, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, TibetTranscriptome

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
11719Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 44395)https://www.dsmz.de/collection/catalogue/details/culture/DSM-44395
19861Wink, J.http://www.dsmz.de/microorganisms/wink_pdf/DSM44395.pdfCompendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
36393Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/6956
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68379Automatically annotated from API Coryne
68382Automatically annotated from API zym
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
77877Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID297260.1StrainInfo: A central database for resolving microbial strain identifiers
121007Curators of the CIPCollection of Institut Pasteur (CIP 109237)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20109237