Strain identifier
BacDive ID: 8479
Type strain:
Species: Mycobacterium murale
Strain Designation: 220-96, MA 112/96
Strain history: CIP <- 1999, CCUG <- M. Salkinoja-Salonen, Helsinki, Finland <- M. A. Andersson
NCBI tax ID(s): 182220 (species)
General
@ref: 11686
BacDive-ID: 8479
DSM-Number: 44340
keywords: genome sequence, 16S sequence, Bacteria, aerobe, mesophilic
description: Mycobacterium murale 220-96 is an aerobe, mesophilic bacterium that was isolated from water-damaged indoor building material.
NCBI tax id
- NCBI tax id: 182220
- Matching level: species
strain history
@ref | history |
---|---|
11686 | <- R. Vuorio; MA 112/96 <- M. Andersson |
67770 | CCUG 39728 <-- M. Salkinoja-Salonen MA112/96. |
121186 | CIP <- 1999, CCUG <- M. Salkinoja-Salonen, Helsinki, Finland <- M. A. Andersson |
doi: 10.13145/bacdive8479.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/actinomycetota
- domain: Bacteria
- phylum: Actinomycetota
- class: Actinomycetes
- order: Mycobacteriales
- family: Mycobacteriaceae
- genus: Mycobacterium
- species: Mycobacterium murale
- full scientific name: Mycobacterium murale Vuorio et al. 1999
synonyms
- @ref: 20215
- synonym: Mycolicibacterium murale
@ref: 11686
domain: Bacteria
phylum: Actinobacteria
class: Actinobacteria
order: Actinomycetales
family: Mycobacteriaceae
genus: Mycobacterium
species: Mycobacterium murale
full scientific name: Mycobacterium murale Vuorio et al. 1999
strain designation: 220-96, MA 112/96
type strain: yes
Morphology
cell morphology
@ref | motility | confidence | gram stain | cell shape |
---|---|---|---|---|
69480 | no | 95.855 | ||
69480 | 100 | positive | ||
121186 | no | rod-shaped |
colony morphology
@ref | colony color | incubation period | medium used |
---|---|---|---|
20046 | Golden yellow (1004) | 10-14 days | 5006 |
20046 | 10-14 days | MB7H10 | |
20046 | 10-14 days | MB7H11 | |
54907 | 2-4 days |
Culture and growth conditions
culture medium
@ref | name | growth | link | composition |
---|---|---|---|---|
11686 | MYCOBACTERIUM MEDIUM (DSMZ Medium 219) | yes | https://mediadive.dsmz.de/medium/219 | Name: MYCOBACTERIUM MEDIUM (DSMZ Medium 219) Composition: Agar 20.0 g/l Na2HPO4 x 12 H2O 2.5 g/l Yeast extract 2.0 g/l Proteose peptone no. 3 2.0 g/l Casein peptone 2.0 g/l Sodium citrate 1.5 g/l KH2PO4 1.0 g/l MgSO4 x 7 H2O 0.6 g/l Tween 0.5 g/l Glycerol Distilled water |
11686 | PEPTONE MEAT EXTRACT GLYCEROL AGAR (DSMZ Medium 250) | yes | https://mediadive.dsmz.de/medium/250 | Name: PEPTONE MEAT EXTRACT GLYCEROL AGAR (DSMZ Medium 250) Composition: Agar 20.0 g/l Proteose peptone no. 3 5.0 g/l Meat extract 3.0 g/l Glycerol Distilled water |
20046 | MB7H10 | yes | Name: Middlebrook 7H10 (MB7H10) Broth Base Composition: amount in percent Middlebrook 7H10 Agar 1.9% Glycerol 0.5% OADC-Enrichment in ml (NL5633) 10ml Comments: Add broth base and glycerol to ddh2O. Adjust volumes. Autoclave. Cool to 42°C. Add OADC-stock. Sterilisation: 20 minutes Temperature:121°C PH before: PH after: Biological details Culture type: suitable for: Mycobacteria OADC stock solution old broth-name: OADC-Stock Composition: Bovine Serum Albumin, fraction V 5 % 1% Oleic Acid in 0.2N NaOH 5ml Glucose 2 % NaCl 0.85 % Comments: Melt 1% Oleic Acid in 65°C water bath. Dissolve all ingredients in 700ml of ddH2O. Adjust volume to 1L. Filter sterilize and store at 4°C. -To make 1% Oleic Acid in 0.2N NaOH: Dissolve 5g oleic acid (ampule) in 500ml 0.2N NaOH (300ml ddH2O + 20ml NaOH + adjust volume to 500ml). Heat solution to 55°C for oleic acid to melt. Store at -20°C in 50ml aliquots. There is no need to sterilize this. Sterilisation: 20 minutes Temperature:121°C PH before: PH after: Biological details Culture type: suitable for: Mycobacteria | |
20046 | MB7H11 | yes | Name: Middlebrook 7H11 (MB7H11) Broth Base Composition: Middlebrook 7H11 Agar 2% Glycerol 0.5% OADC-Enrichment 10ml Comments: Add broth base and glycerol to ddH2O. Adjust volumes. Autoclave. Cool to 42°C. Add OADC-Stock. Sterilisation: 20 minutes Temperature:121°C PH before: PH after: Biological details Culture type: suitable for: Mycobacteria OADC stock solution old broth-name: OADC-Stock Composition: Bovine Serum Albumin, fraction V 5 % 1% Oleic Acid in 0.2N NaOH 5ml Glucose 2 % NaCl 0.85 % Comments: Melt 1% Oleic Acid in 65°C water bath. Dissolve all ingredients in 700ml of ddH2O. Adjust volume to 1L. Filter sterilize and store at 4°C. -To make 1% Oleic Acid in 0.2N NaOH: Dissolve 5g oleic acid (ampule) in 500ml 0.2N NaOH (300ml ddH2O + 20ml NaOH + adjust volume to 500ml). Heat solution to 55°C for oleic acid to melt. Store at -20°C in 50ml aliquots. There is no need to sterilize this. Sterilisation: 20 minutes Temperature:121°C PH before: PH after: Biological details Culture type: suitable for: Mycobacteria | |
20046 | 5006 | yes | Medium: 5006 Name: Composition (g/l) Sucrose 3,0 Dextrin 15,0 Meat extract 1,0 Yeast extract 2,0 Tryptone soy broth (Oxoid) 5,0 NaCl 0,5 K2HPO4 0,5 MgSO4 x 7 H2O 0,5 FeSO4 x 7 H2O 0,01 Agar 20,0 Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7,3 Usage: Maintenance Organisms: All Actinomycetes | |
36255 | MEDIUM 55 - for Mycobacterium | yes | ||
121186 | CIP Medium 55 | yes | https://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=55 |
culture temp
@ref | growth | type | temperature | range |
---|---|---|---|---|
20046 | positive | optimum | 37 | mesophilic |
11686 | positive | growth | 37 | mesophilic |
36255 | positive | growth | 22 | psychrophilic |
54907 | positive | growth | 30 | mesophilic |
67770 | positive | growth | 37 | mesophilic |
121186 | positive | growth | 10-30 | |
121186 | no | growth | 45 | thermophilic |
Physiology and metabolism
oxygen tolerance
@ref | oxygen tolerance |
---|---|
54907 | aerobe |
121186 | obligate aerobe |
spore formation
@ref | spore formation | confidence |
---|---|---|
69481 | no | 93 |
69480 | no | 99.751 |
observation
- @ref: 67770
- observation: quinones: MK-9(H2) (90%), MK-8(H2) (10%)
metabolite utilization
@ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
---|---|---|---|---|
121186 | 606565 | hippurate | - | hydrolysis |
121186 | 17632 | nitrate | - | reduction |
121186 | 16301 | nitrite | - | reduction |
121186 | 17632 | nitrate | - | respiration |
68379 | 17632 | nitrate | + | reduction |
68379 | 4853 | esculin | - | hydrolysis |
68379 | 16199 | urea | + | hydrolysis |
68379 | 5291 | gelatin | + | hydrolysis |
68379 | 17634 | D-glucose | + | fermentation |
68379 | 16988 | D-ribose | - | fermentation |
68379 | 65327 | D-xylose | - | fermentation |
68379 | 16899 | D-mannitol | + | fermentation |
68379 | 17306 | maltose | + | fermentation |
68379 | 17716 | lactose | + | fermentation |
68379 | 17992 | sucrose | + | fermentation |
68379 | 28087 | glycogen | - | fermentation |
metabolite production
- @ref: 121186
- Chebi-ID: 35581
- metabolite: indole
- production: no
enzymes
@ref | value | activity | ec |
---|---|---|---|
68379 | gelatinase | + | |
68379 | urease | + | 3.5.1.5 |
68379 | beta-glucosidase | - | 3.2.1.21 |
68379 | N-acetyl-beta-glucosaminidase | - | 3.2.1.52 |
68379 | alpha-glucosidase | - | 3.2.1.20 |
68379 | beta-galactosidase | + | 3.2.1.23 |
68379 | beta-glucuronidase | + | 3.2.1.31 |
68379 | alkaline phosphatase | - | 3.1.3.1 |
68379 | pyrrolidonyl arylamidase | - | 3.4.19.3 |
68379 | pyrazinamidase | - | 3.5.1.B15 |
121186 | oxidase | - | |
121186 | beta-galactosidase | + | 3.2.1.23 |
121186 | gelatinase | - | |
121186 | amylase | - | |
121186 | DNase | - | |
121186 | caseinase | - | 3.4.21.50 |
121186 | catalase | + | 1.11.1.6 |
121186 | tween esterase | + | |
121186 | gamma-glutamyltransferase | - | 2.3.2.2 |
121186 | lecithinase | - | |
121186 | lipase | - | |
121186 | protease | - | |
121186 | urease | + | 3.5.1.5 |
68382 | esterase (C 4) | + | |
68382 | esterase lipase (C 8) | + | |
68382 | lipase (C 14) | - | |
68382 | alpha-chymotrypsin | - | 3.4.21.1 |
68382 | alpha-galactosidase | - | 3.2.1.22 |
68382 | beta-glucosidase | + | 3.2.1.21 |
68382 | N-acetyl-beta-glucosaminidase | - | 3.2.1.52 |
68382 | alpha-mannosidase | - | 3.2.1.24 |
68382 | alpha-fucosidase | - | 3.2.1.51 |
fatty acid profile
fatty acids
@ref fatty acid percentage ECL 54907 C14:0 4.3 14 54907 C16:0 19.3 16 54907 C18:0 0.6 18 54907 C16:1 ω7c 2.6 15.819 54907 C16:1 ω7c/C15:0 ISO 2OH 10.8 15.85 54907 C16:1 ω9c 1.3 15.774 54907 C17:1 ω7c 16.1 16.818 54907 C17:1 ω8c 0.4 16.792 54907 C18:1 ω9c 28.2 17.769 54907 C18:2 ω6,9c/C18:0 ANTE 0.7 17.724 54907 C20:0 alcohol (C20:0 2OH alc) 11.4 18.835 54907 TBSA 10Me18:0 4.3 18.392 - type of FA analysis: whole cell analysis
- method/protocol: CCUG
API coryne
@ref | NIT | PYZ | PYRA | PAL | beta GUR | beta GAL | alpha GLU | beta NAG | ESC | URE | GEL | GLU | RIB | XYL | MAN | MAL | LAC | SAC | GLYG |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
20046 | + | - | - | - | + | + | - | - | - | + | + | + | - | - | + | + | + | + | - |
API zym
@ref | Alkaline phosphatase | Esterase | Esterase Lipase | Lipase | Leucine arylamidase | Valine arylamidase | Cystine arylamidase | Trypsin | alpha- Chymotrypsin | Acid phosphatase | Naphthol-AS-BI-phosphohydrolase | alpha- Galactosidase | beta- Galactosidase | beta- Glucuronidase | alpha- Glucosidase | beta- Glucosidase | N-acetyl-beta- glucosaminidase | alpha- Mannosidase | alpha- Fucosidase | Control |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
20046 | - | + | + | - | - | - | - | - | - | - | - | - | + | + | - | + | - | - | - | |
121186 | + | + | + | - | + | + | + | + | - | + | + | - | - | - | + | + | - | - | - | - |
API biotype100
@ref | GLU | FRU | GAL | TRE | MNE | SBE | MEL | SAC | RAF | MTE | MAL | LAC | LTE | MbGa | MaGa | CEL | GEN | MbGu | ESC | RIB | ARA | XYL | PLE | RHA | FUC | MLZ | DARL | LARL | XLT | DUL | TAG | GLY | INO | MAN | MTL | TUR | SOR | ADO | HBG | LYX | ERY | MDG | 3MDG | SAT | MUC | LTAT | DTAT | MTAT | DMLT | LMLT | CATE | TATE | TTE | CIT | GRT | GAT | 2KG | 5KG | TRY | NAG | GNT | PAC | PAT | pOBE | QAT | GTE | mOBE | BAT | PPAT | CMT | TGE | BET | PCE | ABT | HIN | LAT | CAP | CYT | HIS | SUC | FUM | GRE | GYT | AVT | ETN | TTN | GLN | ITA | 3OBU | APT | GTT | PRO | DALA | LALA | SER | MNT | PROP | TYR | 2KT |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
121186 | + | + | - | - | - | - | - | - | - | - | + | - | - | - | - | - | - | - | - | + | + | - | - | + | - | - | + | + | + | - | - | - | - | + | - | - | - | + | - | - | - | - | - | - | - | - | - | - | + | + | + | + | - | - | - | - | + | - | - | - | + | - | - | - | - | - | - | - | - | - | - | - | + | - | - | + | - | - | - | + | + | + | - | - | - | - | - | - | + | - | - | - | - | - | - | - | - | - | + |
Isolation, sampling and environmental information
isolation
@ref | sample type | country | origin.country | continent | geographic location |
---|---|---|---|---|---|
11686 | water-damaged indoor building material | Finland | FIN | Europe | |
54907 | Water-damaged indoor building material | Finland | FIN | Europe | |
67770 | Water-damaged indoor building material of a children's day care center | Finland | FIN | Europe | |
121186 | Environment, Water-damaged indoor building material | Finland | FIN | Europe | Helsinki |
isolation source categories
- Cat1: #Engineered
- Cat2: #Built environment
- Cat3: #Indoor
Safety information
risk assessment
@ref | biosafety level | biosafety level comment |
---|---|---|
11686 | 1 | Risk group (German classification) |
20046 | 1 | German classification |
121186 | 1 | Risk group (French classification) |
Sequence information
16S sequences
@ref | description | accession | length | database | NCBI tax ID |
---|---|---|---|---|---|
20218 | Mycobacterium murale 16S ribosomal RNA gene, partial sequence | HM770863 | 1472 | ena | 182220 |
20218 | Mycobacterium murale strain CIP 105980 16S ribosomal RNA gene, partial sequence | AF547950 | 540 | ena | 182220 |
20218 | Mycobacterium murale gene for 16S ribosomal RNA, partial sequence | AB537171 | 1459 | ena | 182220 |
Genome sequences
@ref | description | accession | assembly level | database | NCBI tax ID |
---|---|---|---|---|---|
66792 | Mycolicibacterium murale JCM 13392 | GCA_010722995 | contig | ncbi | 182220 |
66792 | Mycolicibacterium murale strain JCM 13392 | 182220.8 | wgs | patric | 182220 |
66792 | Mycolicibacterium murale DSM 44340 | GCA_025822305 | scaffold | ncbi | 182220 |
GC content
@ref | GC-content | method |
---|---|---|
11686 | 72.9 | |
67770 | 72.9 | high performance liquid chromatography (HPLC) |
Genome-based predictions
predictions
trait | prediction | confidence | training_data |
---|---|---|---|
spore-forming | no | 93 | no |
motile | no | 89.676 | no |
gram-positive | yes | 87.786 | no |
anaerobic | no | 99.487 | yes |
aerobic | yes | 90.519 | yes |
halophile | no | 86.568 | no |
spore-forming | no | 84.81 | no |
glucose-util | yes | 88.632 | no |
flagellated | no | 97.504 | no |
thermophile | no | 99.581 | no |
glucose-ferment | no | 90.401 | no |
External links
@ref: 11686
culture collection no.: CCUG 39728, JCM 13392, DSM 44340, HAMBI 2320, CIP 105980, VTT E-062961
straininfo link
- @ref: 77874
- straininfo: 50580
literature
topic | Pubmed-ID | title | authors | journal | DOI | year | mesh | topic2 |
---|---|---|---|---|---|---|---|---|
Phylogeny | 10028244 | A new rapidly growing mycobacterial species, Mycobacterium murale sp. nov., isolated from the indoor walls of a children's day care centre. | Vuorio R, Andersson MA, Rainey FA, Kroppenstedt RM, Kampfer P, Busse HJ, Viljanen M, Salkinoja-Salonen M | Int J Syst Bacteriol | 10.1099/00207713-49-1-25 | 1999 | Base Sequence, Child, Child Day Care Centers, DNA, Ribosomal/chemistry, *Environmental Microbiology, Humans, Microbial Sensitivity Tests, Molecular Sequence Data, Mycobacterium/chemistry/*classification/drug effects, RNA, Ribosomal, 16S/genetics | Pathogenicity |
Phylogeny | 34878372 | Mycolicibacterium baixiangningiae sp. nov. and Mycolicibacterium mengxianglii sp. nov., two new rapidly growing mycobacterial species. | Cheng Y, Lei W, Wang X, Tian Z, Liu H, Yang J, Lu S, Lai XH, Pu J, Huang Y, Zhang S, Yang C, Lian X, Bai Y, Wan K, Wang S, Xu J | Int J Syst Evol Microbiol | 10.1099/ijsem.0.005019 | 2021 | Animals, Antelopes/*microbiology, Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Feces/microbiology, Mycobacteriaceae/*classification/isolation & purification, Nucleic Acid Hybridization, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Tibet | Transcriptome |
Reference
@id | authors | catalogue | doi/url | title | journal | pubmed |
---|---|---|---|---|---|---|
11686 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 44340) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-44340 | |||
20046 | Wink, J. | http://www.dsmz.de/microorganisms/wink_pdf/DSM44340.pdf | Compendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig | |||
20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |||
20218 | Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P. | 10.1016/j.syapm.2013.11.002 | StrainInfo introduces electronic passports for microorganisms. | Syst Appl Microbiol. 37: 42-50 2014 | 24321274 | |
36255 | Curators of the CIP | https://brclims.pasteur.fr/brcWeb/souche/detail/1/18059 | ||||
54907 | Curators of the CCUG | Culture Collection University of Gothenburg (CCUG) (CCUG 39728) | https://www.ccug.se/strain?id=39728 | |||
66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |||
67770 | Curators of the JCM | https://jcm.brc.riken.jp/en/ | ||||
68379 | Automatically annotated from API Coryne | |||||
68382 | Automatically annotated from API zym | |||||
69480 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |||
69481 | Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch | 10.21203/rs.3.rs-2527258/v1 | Predictions based on the model GenomeNet Sporulation v. 1 | |||
77874 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID50580.1 | StrainInfo: A central database for resolving microbial strain identifiers | |||
121186 | Curators of the CIP | Collection of Institut Pasteur (CIP 105980) | https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20105980 |