Strain identifier
BacDive ID: 8477
Type strain:
Species: Mycobacterium tusciae
Strain Designation: Fi-25796, ID 3930
Strain history: CIP <- 2000, E. Tortoli, Florence, Italy: strain FI-25796 <- Inst. Hyg., Pisa, Italy
NCBI tax ID(s): 75922 (species)
General
@ref: 11684
BacDive-ID: 8477
DSM-Number: 44338
keywords: genome sequence, 16S sequence, Bacteria, mesophilic, rod-shaped
description: Mycobacterium tusciae Fi-25796 is a mesophilic, rod-shaped bacterium that was isolated from cervical lymph node of a child.
NCBI tax id
- NCBI tax id: 75922
- Matching level: species
strain history
@ref | history |
---|---|
11684 | <- E. Tortoli, Coreggi Hospital, Fi-25796 <- Hygiene Inst. Pisa |
67770 | CIP 106367 <-- E. Tortoli FI-25796 <-- Inst. Hyg., Pisa, Italy. |
123700 | CIP <- 2000, E. Tortoli, Florence, Italy: strain FI-25796 <- Inst. Hyg., Pisa, Italy |
doi: 10.13145/bacdive8477.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/actinomycetota
- domain: Bacteria
- phylum: Actinomycetota
- class: Actinomycetes
- order: Mycobacteriales
- family: Mycobacteriaceae
- genus: Mycobacterium
- species: Mycobacterium tusciae
- full scientific name: Mycobacterium tusciae Tortoli et al. 1999
synonyms
- @ref: 20215
- synonym: Mycolicibacterium tusciae
@ref: 11684
domain: Bacteria
phylum: Actinobacteria
class: Actinobacteria
order: Actinomycetales
family: Mycobacteriaceae
genus: Mycobacterium
species: Mycobacterium tusciae
full scientific name: Mycobacterium tusciae Tortoli et al. 1999
strain designation: Fi-25796, ID 3930
type strain: yes
Morphology
cell morphology
- @ref: 123700
- cell shape: rod-shaped
- motility: no
Culture and growth conditions
culture medium
@ref | name | growth | link | composition |
---|---|---|---|---|
11684 | LÖWENSTEIN-JENSEN MEDIUM (DSMZ Medium 354) | yes | https://mediadive.dsmz.de/medium/354 | Name: LÖWENSTEIN-JENSEN MEDIUM (DSMZ Medium 354) Composition: Potato flour 18.6104 g/l L-Asparagin 2.23325 g/l KH2PO4 1.55087 g/l Mg-citrate 0.372208 g/l Malachite green 0.248139 g/l MgSO4 0.148883 g/l Glycerol Fresh egg mixture Distilled water |
11684 | MIDDLEBROOK MEDIUM (DSMZ Medium 645) | yes | https://mediadive.dsmz.de/medium/645 | Name: MIDDLEBROOK MEDIUM (DSMZ Medium 645) Composition: Bacto Middlebrook 7H10 agar 20.9945 g/l Glycerol Distilled water |
36250 | MEDIUM 55 - for Mycobacterium | yes | ||
123700 | CIP Medium 55 | yes | https://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=55 |
culture temp
@ref | growth | type | temperature | range |
---|---|---|---|---|
11684 | positive | growth | 37 | mesophilic |
36250 | positive | growth | 30 | mesophilic |
67770 | positive | growth | 28 | mesophilic |
123700 | positive | growth | 22-37 | |
123700 | no | growth | 10 | psychrophilic |
123700 | no | growth | 45 | thermophilic |
Physiology and metabolism
metabolite utilization
@ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
---|---|---|---|---|
68369 | 25115 | malate | + | assimilation |
68369 | 27689 | decanoate | - | assimilation |
68369 | 24265 | gluconate | + | assimilation |
68369 | 17306 | maltose | - | assimilation |
68369 | 59640 | N-acetylglucosamine | - | assimilation |
68369 | 30849 | L-arabinose | - | assimilation |
68369 | 17634 | D-glucose | - | assimilation |
68369 | 5291 | gelatin | - | hydrolysis |
68369 | 4853 | esculin | - | hydrolysis |
68369 | 16199 | urea | - | hydrolysis |
68369 | 29016 | arginine | - | hydrolysis |
68369 | 17634 | D-glucose | - | fermentation |
68369 | 27897 | tryptophan | - | energy source |
68369 | 17632 | nitrate | + | reduction |
123700 | 606565 | hippurate | - | hydrolysis |
123700 | 17632 | nitrate | + | reduction |
123700 | 16301 | nitrite | - | reduction |
metabolite production
@ref | Chebi-ID | metabolite | production |
---|---|---|---|
68369 | 35581 | indole | no |
123700 | 35581 | indole | no |
metabolite tests
- @ref: 68369
- Chebi-ID: 35581
- metabolite: indole
- indole test: -
enzymes
@ref | value | activity | ec |
---|---|---|---|
68369 | gelatinase | - | |
68369 | beta-glucosidase | - | 3.2.1.21 |
68369 | urease | - | 3.5.1.5 |
68369 | arginine dihydrolase | - | 3.5.3.6 |
123700 | oxidase | - | |
123700 | beta-galactosidase | - | 3.2.1.23 |
123700 | gelatinase | - | |
123700 | catalase | + | 1.11.1.6 |
123700 | gamma-glutamyltransferase | - | 2.3.2.2 |
123700 | urease | - | 3.5.1.5 |
68382 | esterase lipase (C 8) | + | |
68382 | lipase (C 14) | - | |
68382 | leucine arylamidase | + | 3.4.11.1 |
68382 | valine arylamidase | - | |
68382 | trypsin | - | 3.4.21.4 |
68382 | alpha-chymotrypsin | - | 3.4.21.1 |
68382 | alpha-galactosidase | - | 3.2.1.22 |
68382 | beta-galactosidase | - | 3.2.1.23 |
68382 | beta-glucuronidase | - | 3.2.1.31 |
68382 | alpha-glucosidase | - | 3.2.1.20 |
68382 | beta-glucosidase | - | 3.2.1.21 |
68382 | N-acetyl-beta-glucosaminidase | - | 3.2.1.52 |
68382 | alpha-mannosidase | - | 3.2.1.24 |
68382 | alpha-fucosidase | - | 3.2.1.51 |
API zym
@ref | Control | Alkaline phosphatase | Esterase | Esterase Lipase | Lipase | Leucine arylamidase | Valine arylamidase | Cystine arylamidase | Trypsin | alpha- Chymotrypsin | Acid phosphatase | Naphthol-AS-BI-phosphohydrolase | alpha- Galactosidase | beta- Galactosidase | beta- Glucuronidase | alpha- Glucosidase | beta- Glucosidase | N-acetyl-beta- glucosaminidase | alpha- Mannosidase | alpha- Fucosidase |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
11684 | - | - | +/- | + | - | + | - | - | - | - | - | - | - | - | - | - | - | - | - | - |
123700 | - | + | + | + | - | + | - | + | - | - | + | + | - | - | - | - | - | - | - | - |
API 20NE
@ref | NO3 | TRP | GLU_ Ferm | ADH Arg | URE | ESC | GEL | PNPG | GLU_ Assim | ARA | MNE | MAN | NAG | MAL | GNT | CAP | ADI | MLT | CIT | PAC |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
11684 | + | - | - | - | - | - | - | - | - | - | +/- | +/- | - | - | + | - | +/- | + | - | - |
Isolation, sampling and environmental information
isolation
@ref | sample type | geographic location | country | origin.country | continent | sampling date | isolation date |
---|---|---|---|---|---|---|---|
11684 | cervical lymph node of a child | Pisa | Italy | ITA | Europe | ||
59084 | Human lymph node | Pisa | Italy | ITA | Europe | 1996 | |
67770 | Cervical lymph node of a child | ||||||
123700 | Human, Cervical lymphonode (child) | Pisa | Italy | ITA | Europe | 1995 |
isolation source categories
Cat1 | Cat2 | Cat3 |
---|---|---|
#Infection | #Patient | |
#Host Body-Site | #Organ | #Lymph node |
#Host | #Human | #Child |
Safety information
risk assessment
@ref | biosafety level | biosafety level comment |
---|---|---|
11684 | 1 | Risk group (German classification) |
123700 | 1 | Risk group (French classification) |
Sequence information
16S sequences
@ref | description | accession | length | database | NCBI tax ID |
---|---|---|---|---|---|
20218 | Mycobacterium tusciae strain CIP 106367 16S ribosomal RNA gene, partial sequence | AF547978 | 543 | ena | 75922 |
20218 | Mycobacterium tusciae 16S ribosomal RNA gene, partial sequence | HM770864 | 1472 | ena | 75922 |
11684 | Mycobacterium sp. FI-25796 16S ribosomal RNA gene, complete sequence | AF058299 | 1409 | ena | 75922 |
Genome sequences
@ref | description | accession | assembly level | database | NCBI tax ID |
---|---|---|---|---|---|
66792 | Mycolicibacterium tusciae DSM 44338 | 2865601517 | draft | img | 75922 |
67770 | Mycolicibacterium tusciae DSM 44338 | GCA_002086795 | contig | ncbi | 75922 |
GC content
@ref | GC-content | method |
---|---|---|
11684 | 66.4 | |
67770 | 66.4 | high performance liquid chromatography (HPLC) |
External links
@ref: 11684
culture collection no.: CCUG 50996, CIP 106367, JCM 12692, DSM 44338, ATCC BAA 564
straininfo link
- @ref: 77872
- straininfo: 50578
literature
- topic: Phylogeny
- Pubmed-ID: 10555367
- title: Mycobacterium tusciae sp. nov.
- authors: Tortoli E, Kroppenstedt RM, Bartoloni A, Caroli G, Jan I, Pawlowski J, Emler S
- journal: Int J Syst Bacteriol
- DOI: 10.1099/00207713-49-4-1839
- year: 1999
- mesh: Bacterial Typing Techniques, Base Composition, Base Sequence, Child, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, Fibrosis/microbiology, Humans, Immunocompromised Host, Lipids/analysis, Lymph Nodes/microbiology, Molecular Sequence Data, Mycobacterium/chemistry/*classification/isolation & purification/physiology, Mycobacterium Infections/microbiology, Mycolic Acids/analysis, Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Water Microbiology, Water Supply
- topic2: Pathogenicity
Reference
@id | authors | catalogue | doi/url | title | journal | pubmed |
---|---|---|---|---|---|---|
11684 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 44338) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-44338 | |||
20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |||
20218 | Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P. | 10.1016/j.syapm.2013.11.002 | StrainInfo introduces electronic passports for microorganisms. | Syst Appl Microbiol. 37: 42-50 2014 | 24321274 | |
36250 | Curators of the CIP | https://brclims.pasteur.fr/brcWeb/souche/detail/1/18490 | ||||
59084 | Curators of the CCUG | Culture Collection University of Gothenburg (CCUG) (CCUG 50996) | https://www.ccug.se/strain?id=50996 | |||
66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |||
66794 | Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg | 10.1093/nar/gkaa1025 | BRENDA, the ELIXIR core data resource in 2021: new developments and updates | Nucleic Acids Res. 49: D498-D508 2020 | 33211880 | |
67770 | Curators of the JCM | https://jcm.brc.riken.jp/en/ | ||||
68369 | Automatically annotated from API 20NE | |||||
68382 | Automatically annotated from API zym | |||||
77872 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID50578.1 | StrainInfo: A central database for resolving microbial strain identifiers | |||
123700 | Curators of the CIP | Collection of Institut Pasteur (CIP 106367) | https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20106367 |