Strain identifier

BacDive ID: 8468

Type strain: Yes

Species: Mycobacterium pulveris

Strain Designation: 33505

Strain history: CIP <- 2000, JCM <- M. Tsukamura: strain 33505

NCBI tax ID(s): 36813 (species)

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General

@ref: 11606

BacDive-ID: 8468

DSM-Number: 44222

keywords: genome sequence, 16S sequence, Bacteria, aerobe, mesophilic, Gram-positive

description: Mycobacterium pulveris 33505 is an aerobe, mesophilic, Gram-positive bacterium that was isolated from house dust.

NCBI tax id

  • NCBI tax id: 36813
  • Matching level: species

strain history

@refhistory
11606<- A. F. Yassin <- ATCC <- M. Tsukamura, NCH 33505
67771<- M Tsukamura
67770M. Tsukamura 33505.
122052CIP <- 2000, JCM <- M. Tsukamura: strain 33505

doi: 10.13145/bacdive8468.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Mycobacteriales
  • family: Mycobacteriaceae
  • genus: Mycobacterium
  • species: Mycobacterium pulveris
  • full scientific name: Mycobacterium pulveris Tsukamura et al. 1983

  • synonyms

    • @ref: 20215
    • synonym: Mycolicibacterium pulveris

@ref: 11606

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Mycobacteriaceae

genus: Mycobacterium

species: Mycobacterium pulveris

full scientific name: Mycobacterium pulveris Tsukamura et al. 1983

strain designation: 33505

type strain: yes

Morphology

cell morphology

@refgram stainmotilityconfidencecell shape
67771positive
69480no95.458
69480positive100
122052norod-shaped

colony morphology

@refcolony colorincubation periodmedium used
19852Zinc yellow10-14 days5006
19852Zinc yellow10-14 daysMB7H10
19852Zinc yellow10-14 daysMB7H11
537953 days

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
11606LÖWENSTEIN-JENSEN MEDIUM (DSMZ Medium 354)yeshttps://mediadive.dsmz.de/medium/354Name: LÖWENSTEIN-JENSEN MEDIUM (DSMZ Medium 354) Composition: Potato flour 18.6104 g/l L-Asparagin 2.23325 g/l KH2PO4 1.55087 g/l Mg-citrate 0.372208 g/l Malachite green 0.248139 g/l MgSO4 0.148883 g/l Glycerol Fresh egg mixture Distilled water
11606MIDDLEBROOK MEDIUM (DSMZ Medium 645)yeshttps://mediadive.dsmz.de/medium/645Name: MIDDLEBROOK MEDIUM (DSMZ Medium 645) Composition: Bacto Middlebrook 7H10 agar 20.9945 g/l Glycerol Distilled water
19852MB7H10yesName: Middlebrook 7H10 (MB7H10) Broth Base Composition: amount in percent Middlebrook 7H10 Agar 1.9% Glycerol 0.5% OADC-Enrichment in ml (NL5633) 10ml Comments: Add broth base and glycerol to ddh2O. Adjust volumes. Autoclave. Cool to 42°C. Add OADC-stock. Sterilisation: 20 minutes Temperature:121°C PH before: PH after: Biological details Culture type: suitable for: Mycobacteria OADC stock solution old broth-name: OADC-Stock Composition: Bovine Serum Albumin, fraction V 5 % 1% Oleic Acid in 0.2N NaOH 5ml Glucose 2 % NaCl 0.85 % Comments: Melt 1% Oleic Acid in 65°C water bath. Dissolve all ingredients in 700ml of ddH2O. Adjust volume to 1L. Filter sterilize and store at 4°C. -To make 1% Oleic Acid in 0.2N NaOH: Dissolve 5g oleic acid (ampule) in 500ml 0.2N NaOH (300ml ddH2O + 20ml NaOH + adjust volume to 500ml). Heat solution to 55°C for oleic acid to melt. Store at -20°C in 50ml aliquots. There is no need to sterilize this. Sterilisation: 20 minutes Temperature:121°C PH before: PH after: Biological details Culture type: suitable for: Mycobacteria
19852MB7H11yesName: Middlebrook 7H11 (MB7H11) Broth Base Composition: Middlebrook 7H11 Agar 2% Glycerol 0.5% OADC-Enrichment 10ml Comments: Add broth base and glycerol to ddH2O. Adjust volumes. Autoclave. Cool to 42°C. Add OADC-Stock. Sterilisation: 20 minutes Temperature:121°C PH before: PH after: Biological details Culture type: suitable for: Mycobacteria OADC stock solution old broth-name: OADC-Stock Composition: Bovine Serum Albumin, fraction V 5 % 1% Oleic Acid in 0.2N NaOH 5ml Glucose 2 % NaCl 0.85 % Comments: Melt 1% Oleic Acid in 65°C water bath. Dissolve all ingredients in 700ml of ddH2O. Adjust volume to 1L. Filter sterilize and store at 4°C. -To make 1% Oleic Acid in 0.2N NaOH: Dissolve 5g oleic acid (ampule) in 500ml 0.2N NaOH (300ml ddH2O + 20ml NaOH + adjust volume to 500ml). Heat solution to 55°C for oleic acid to melt. Store at -20°C in 50ml aliquots. There is no need to sterilize this. Sterilisation: 20 minutes Temperature:121°C PH before: PH after: Biological details Culture type: suitable for: Mycobacteria
198525006yesMedium: 5006 Name: Composition (g/l) Sucrose 3,0 Dextrin 15,0 Meat extract 1,0 Yeast extract 2,0 Tryptone soy broth (Oxoid) 5,0 NaCl 0,5 K2HPO4 0,5 MgSO4 x 7 H2O 0,5 FeSO4 x 7 H2O 0,01 Agar 20,0 Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7,3 Usage: Maintenance Organisms: All Actinomycetes
36380MEDIUM 55 - for Mycobacteriumyes
122052CIP Medium 55yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=55

culture temp

@refgrowthtypetemperaturerange
19852positiveoptimum37mesophilic
11606positivegrowth37mesophilic
36380positivegrowth37mesophilic
53795positivegrowth37mesophilic
67770positivegrowth37mesophilic
67771positivegrowth37mesophilic
122052positivegrowth22-45
122052nogrowth10psychrophilic

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
53795aerobe
67771aerobe
122052obligate aerobe

spore formation

@refspore formationconfidence
69481no98
69480no99.979

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
122052606565hippurate-hydrolysis
12205217632nitrate+reduction
12205216301nitrite-reduction
12205217632nitrate-respiration
6837917632nitrate-reduction
683794853esculin-hydrolysis
683795291gelatin+hydrolysis
6837917634D-glucose-fermentation
6837916988D-ribose-fermentation
6837965327D-xylose-fermentation
6837916899D-mannitol-fermentation
6837917306maltose-fermentation
6837917716lactose-fermentation
6837917992sucrose+fermentation
6837928087glycogen-fermentation

metabolite production

  • @ref: 122052
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

enzymes

@refvalueactivityec
68379gelatinase+
68379beta-glucosidase-3.2.1.21
68379N-acetyl-beta-glucosaminidase-3.2.1.52
68379alpha-glucosidase+3.2.1.20
68379beta-galactosidase-3.2.1.23
68379beta-glucuronidase-3.2.1.31
68379pyrrolidonyl arylamidase+3.4.19.3
68379pyrazinamidase+3.5.1.B15
122052oxidase-
122052beta-galactosidase-3.2.1.23
122052gelatinase-
122052catalase+1.11.1.6
122052gamma-glutamyltransferase-2.3.2.2
122052urease+3.5.1.5
68382alkaline phosphatase+3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)+
68382leucine arylamidase+3.4.11.1
68382valine arylamidase-
68382trypsin-3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase-3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382beta-glucosidase-3.2.1.21
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API coryne

@refNITPYZPYRAPALbeta GURbeta GALalpha GLUbeta NAGESCUREGELGLURIBXYLMANMALLACSACGLYG
19852-+++/---+--+/-+------+-

API zym

@refAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- FucosidaseControl
19852+++++-+--++---+----
122052+++-+----++---------

Isolation, sampling and environmental information

isolation

@refsample type
11606house dust
53795House dust
67770House dust
67771From house dust
122052Environment, House dust

isolation source categories

Cat1Cat2Cat3
#Engineered#Built environment#House
#Engineered#Waste#Dust (Ash)

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
116061Risk group (German classification)
198521Risk group (German classification)
1220521Risk group (French classification)

Sequence information

16S sequences

  • @ref: 11606
  • description: Mycobacterium pulveris 16S rRNA gene, strain DSM 44222T
  • accession: AJ429046
  • length: 1492
  • database: ena
  • NCBI tax ID: 36813

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Mycolicibacterium pulveris JCM 6370GCA_010725725completencbi36813
66792Mycolicibacterium pulveris strain JCM 637036813.7completepatric36813
66792Mycolicibacterium pulveris DSM 44222GCA_025822105scaffoldncbi36813

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno98no
flagellatedno98.736no
gram-positiveyes89.905yes
anaerobicno99.068yes
aerobicyes85.771no
halophileno82.607no
spore-formingno81.918no
glucose-utilyes78.678no
thermophileno98.556yes
motileno94.208no
glucose-fermentno90.663yes

External links

@ref: 11606

culture collection no.: CCUG 37668, CIP 106804, KCTC 9518, DSM 44222, ATCC 35154, CIP 1480001, JCM 6370, NCH 33505

straininfo link

  • @ref: 77863
  • straininfo: 40892

Reference

@idauthorscataloguedoi/urltitle
11606Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 44222)https://www.dsmz.de/collection/catalogue/details/culture/DSM-44222
19852Wink, J.http://www.dsmz.de/microorganisms/wink_pdf/DSM44222.pdfCompendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
36380Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/18974
53795Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 37668)https://www.ccug.se/strain?id=37668
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
67771Curators of the KCTChttps://kctc.kribb.re.kr/En/Kctc
68379Automatically annotated from API Coryne
68382Automatically annotated from API zym
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
77863Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID40892.1StrainInfo: A central database for resolving microbial strain identifiers
122052Curators of the CIPCollection of Institut Pasteur (CIP 106804)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20106804