Strain identifier

BacDive ID: 8467

Type strain: Yes

Species: Mycobacterium moriokaense

Strain Designation: 14 147 0001, 48504

Strain history: CIP <- 1998, V. Vincent, Inst. Pasteur, Paris, France: strain 14 147 0001 <- ATCC <- M. Tsukamura: strain NCH E11715 (= 48504)

NCBI tax ID(s): 39691 (species)

For citation purpose refer to the digital object identifier (doi) of the current version.
Archive
version 8.1 (current version):
version 8:
version 7.1:
version 7:
version 6:
version 5:
version 4.1:
version 4:
version 3:
version 2.1:
version 2:
version 1:
version 8.1 (current version)

General

@ref: 11605

BacDive-ID: 8467

DSM-Number: 44221

keywords: genome sequence, 16S sequence, Bacteria, aerobe, mesophilic, human pathogen

description: Mycobacterium moriokaense 14 147 0001 is an aerobe, mesophilic human pathogen that was isolated from soil.

NCBI tax id

  • NCBI tax id: 39691
  • Matching level: species

strain history

@refhistory
11605<- M. Goodfellow, OI MOR <- JCM <- M. Tsukamura, NCH E11715 (=strain 48504)
67770M. Tsukamura E11715 (=48504).
121145CIP <- 1998, V. Vincent, Inst. Pasteur, Paris, France: strain 14 147 0001 <- ATCC <- M. Tsukamura: strain NCH E11715 (= 48504)

doi: 10.13145/bacdive8467.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Mycobacteriales
  • family: Mycobacteriaceae
  • genus: Mycobacterium
  • species: Mycobacterium moriokaense
  • full scientific name: Mycobacterium moriokaense Tsukamura et al. 1986
  • synonyms

    • @ref: 20215
    • synonym: Mycolicibacterium moriokaense

@ref: 11605

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Mycobacteriaceae

genus: Mycobacterium

species: Mycobacterium moriokaense

full scientific name: Mycobacterium moriokaense Tsukamura et al. 1986 emend. Nouioui et al. 2018

strain designation: 14 147 0001, 48504

type strain: yes

Morphology

cell morphology

@refmotilityconfidencegram staincell shape
69480no91.04
69480100positive
121145norod-shaped

colony morphology

@refcolony colorincubation periodmedium used
20020Oyster white (1013)10-14 days5006
20020Oyster white (1013)MB7H10
20020Oyster white (1013)10-14 daysMB7H11
537982 days

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
11605LÖWENSTEIN-JENSEN MEDIUM (DSMZ Medium 354)yeshttps://mediadive.dsmz.de/medium/354Name: LÖWENSTEIN-JENSEN MEDIUM (DSMZ Medium 354) Composition: Potato flour 18.6104 g/l L-Asparagin 2.23325 g/l KH2PO4 1.55087 g/l Mg-citrate 0.372208 g/l Malachite green 0.248139 g/l MgSO4 0.148883 g/l Glycerol Fresh egg mixture Distilled water
11605MIDDLEBROOK MEDIUM (DSMZ Medium 645)yeshttps://mediadive.dsmz.de/medium/645Name: MIDDLEBROOK MEDIUM (DSMZ Medium 645) Composition: Bacto Middlebrook 7H10 agar 20.9945 g/l Glycerol Distilled water
20020MB7H10yesName: Middlebrook 7H10 (MB7H10) Broth Base Composition: amount in percent Middlebrook 7H10 Agar 1.9% Glycerol 0.5% OADC-Enrichment in ml (NL5633) 10ml Comments: Add broth base and glycerol to ddh2O. Adjust volumes. Autoclave. Cool to 42°C. Add OADC-stock. Sterilisation: 20 minutes Temperature:121°C PH before: PH after: Biological details Culture type: suitable for: Mycobacteria OADC stock solution old broth-name: OADC-Stock Composition: Bovine Serum Albumin, fraction V 5 % 1% Oleic Acid in 0.2N NaOH 5ml Glucose 2 % NaCl 0.85 % Comments: Melt 1% Oleic Acid in 65°C water bath. Dissolve all ingredients in 700ml of ddH2O. Adjust volume to 1L. Filter sterilize and store at 4°C. -To make 1% Oleic Acid in 0.2N NaOH: Dissolve 5g oleic acid (ampule) in 500ml 0.2N NaOH (300ml ddH2O + 20ml NaOH + adjust volume to 500ml). Heat solution to 55°C for oleic acid to melt. Store at -20°C in 50ml aliquots. There is no need to sterilize this. Sterilisation: 20 minutes Temperature:121°C PH before: PH after: Biological details Culture type: suitable for: Mycobacteria
20020MB7H11yesName: Middlebrook 7H11 (MB7H11) Broth Base Composition: Middlebrook 7H11 Agar 2% Glycerol 0.5% OADC-Enrichment 10ml Comments: Add broth base and glycerol to ddH2O. Adjust volumes. Autoclave. Cool to 42°C. Add OADC-Stock. Sterilisation: 20 minutes Temperature:121°C PH before: PH after: Biological details Culture type: suitable for: Mycobacteria OADC stock solution old broth-name: OADC-Stock Composition: Bovine Serum Albumin, fraction V 5 % 1% Oleic Acid in 0.2N NaOH 5ml Glucose 2 % NaCl 0.85 % Comments: Melt 1% Oleic Acid in 65°C water bath. Dissolve all ingredients in 700ml of ddH2O. Adjust volume to 1L. Filter sterilize and store at 4°C. -To make 1% Oleic Acid in 0.2N NaOH: Dissolve 5g oleic acid (ampule) in 500ml 0.2N NaOH (300ml ddH2O + 20ml NaOH + adjust volume to 500ml). Heat solution to 55°C for oleic acid to melt. Store at -20°C in 50ml aliquots. There is no need to sterilize this. Sterilisation: 20 minutes Temperature:121°C PH before: PH after: Biological details Culture type: suitable for: Mycobacteria
200205006yesMedium: 5006 Name: Composition (g/l) Sucrose 3,0 Dextrin 15,0 Meat extract 1,0 Yeast extract 2,0 Tryptone soy broth (Oxoid) 5,0 NaCl 0,5 K2HPO4 0,5 MgSO4 x 7 H2O 0,5 FeSO4 x 7 H2O 0,01 Agar 20,0 Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7,3 Usage: Maintenance Organisms: All Actinomycetes
36368MEDIUM 55 - for Mycobacteriumyes
121145CIP Medium 55yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=55

culture temp

@refgrowthtypetemperaturerange
20020positiveoptimum37mesophilic
11605positivegrowth37mesophilic
36368positivegrowth37mesophilic
53798positivegrowth30-37mesophilic
67770positivegrowth37mesophilic
121145positivegrowth25-37mesophilic
121145nogrowth10psychrophilic
121145nogrowth45thermophilic

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
53798aerobe
121145obligate aerobe

spore formation

  • @ref: 69480
  • spore formation: no
  • confidence: 99.901

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
121145606565hippurate-hydrolysis
12114517632nitrate+reduction
12114516301nitrite-reduction
12114517632nitrate-respiration
683794853esculin-hydrolysis
683795291gelatin-hydrolysis
6837917634D-glucose-fermentation
6837916988D-ribose-fermentation
6837965327D-xylose-fermentation
6837916899D-mannitol-fermentation
6837917306maltose-fermentation
6837917716lactose-fermentation
6837928087glycogen-fermentation

metabolite production

  • @ref: 121145
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

enzymes

@refvalueactivityec
68379gelatinase-
68379beta-glucosidase-3.2.1.21
68379N-acetyl-beta-glucosaminidase-3.2.1.52
68379alpha-glucosidase+3.2.1.20
68379beta-galactosidase-3.2.1.23
68379beta-glucuronidase-3.2.1.31
68379alkaline phosphatase-3.1.3.1
121145oxidase-
121145beta-galactosidase-3.2.1.23
121145gelatinase-
121145amylase-
121145DNase-
121145caseinase-3.4.21.50
121145catalase+1.11.1.6
121145tween esterase-
121145gamma-glutamyltransferase-2.3.2.2
121145lecithinase-
121145lipase-
121145urease+3.5.1.5
68382alkaline phosphatase-3.1.3.1
68382esterase lipase (C 8)+
68382lipase (C 14)-
68382leucine arylamidase+3.4.11.1
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase-3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-fucosidase-3.2.1.51

API coryne

@refNITPYZPYRAPALbeta GURbeta GALalpha GLUbeta NAGESCUREGELGLURIBXYLMANMALLACSACGLYGControl
20020-++---+--+-------+-
11605++/-----+-------------

API zym

@refAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- FucosidaseControl
20020-++-++++++----++-+-
11605-+/-+-+-+/-+/-------------
121145-++-+----++---------

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
121145+-------------+-----------+------+--+------------+----------+-----------+--+--+---------+-------+--

Isolation, sampling and environmental information

isolation

@refsample typecountryorigin.countrycontinent
11605soilJapanJPNAsia
53798Soil
67770Soil
121145Environment, SoilJapanJPNAsia

isolation source categories

  • Cat1: #Environmental
  • Cat2: #Terrestrial
  • Cat3: #Soil

Safety information

risk assessment

@refpathogenicity humanbiosafety levelbiosafety level comment
11605yes, in single cases1Risk group (German classification)
200201German classification
1211451Risk group (French classification)

Sequence information

16S sequences

  • @ref: 11605
  • description: Mycobacterium moriokaense 16S rRNA gene, strain DSM 44221T
  • accession: AJ429044
  • length: 1493
  • database: ena
  • NCBI tax ID: 39691

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Mycolicibacterium moriokaense JCM 6375GCA_010726085completencbi39691
66792Mycolicibacterium moriokaense DSM 44221GCA_003347205contigncbi39691
66792Mycobacterium moriokaense strain CIP10539339691.4wgspatric39691
66792Mycobacterium moriokaense strain CIP10539339691.3wgspatric39691
66792Mycolicibacterium moriokaense strain DSM 4422139691.9wgspatric39691
66792Mycolicibacterium moriokaense strain JCM 637539691.13completepatric39691
66792Mycolicibacterium moriokaense CIP1053932865562250draftimg39691
66792Mycolicibacterium moriokaense DSM 442212856244778draftimg39691
67770Mycolicibacterium moriokaense CIP105393GCA_002086395contigncbi39691
66792Mycolicibacterium moriokaense DSM 44221GCA_025822185contigncbi39691

GC content

@refGC-contentmethod
6777064thermal denaturation, midpoint method (Tm)
6777066genome sequence analysis

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno86no
motileno93.696no
flagellatedno98.496no
gram-positiveyes90.008no
anaerobicno99.282yes
aerobicyes92.629yes
halophileno93.962no
spore-formingno87.971no
glucose-utilyes82.544no
thermophileno99.567no
glucose-fermentno89.383yes

External links

@ref: 11605

culture collection no.: DSM 44221, ATCC 43059, JCM 6375, CCUG 37671, CIP 105393, KCTC 9516, VKM Ac-1183, NCH E11715

straininfo link

  • @ref: 77862
  • straininfo: 41622

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny30696899Genome-guided and mass spectrometry investigation of natural products produced by a potential new actinobacterial strain isolated from a mangrove ecosystem in Futian, Shenzhen, China.Hu D, Gao C, Sun C, Jin T, Fan G, Mok KM, Lee SMSci Rep10.1038/s41598-018-37475-w2019Anti-Bacterial Agents/isolation & purification/metabolism/*pharmacology, Bacterial Typing Techniques, Biological Products/isolation & purification/metabolism/*pharmacology, China, DNA, Bacterial/genetics, Genome, Bacterial/*genetics, Mycobacterium/classification/*genetics/isolation & purification/*metabolism, RNA, Ribosomal, 16S/genetics, Secondary Metabolism/physiology, Sequence Analysis, DNA, Soil Microbiology, Wetlands, Whole Genome SequencingMetabolism
Phylogeny31460855Mycolicibacterium stellerae sp. nov., a rapidly growing scotochromogenic strain isolated from Stellera chamaejasme.Nouioui I, Sangal V, Cortes-Albayay C, Jando M, Igual JM, Klenk HP, Zhang YQ, Goodfellow MInt J Syst Evol Microbiol10.1099/ijsem.0.0036442019Bacterial Typing Techniques, Base Composition, China, DNA, Bacterial/genetics, Fatty Acids/chemistry, Mycobacteriaceae/*classification/isolation & purification, Nucleic Acid Hybridization, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Thymelaeaceae/*microbiologyTranscriptome
Phylogeny32054545Phenotypic and molecular characterisation of a novel species, Mycobacterium hubeiense sp., isolated from the sputum of a patient with secondary tuberculosis in Hubei of China.Yu X, Zheng H, Zhou F, Hu P, Wang H, Li N, He J, Wang P, Zhang L, Men H, Xiang J, Zhang SEpidemiol Infect10.1017/S09502688200004362020Aged, 80 and over, Antitubercular Agents/pharmacology, Bacterial Proteins/genetics, Base Composition, Chaperonin 60/genetics, China, Cluster Analysis, Cytosol/chemistry, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, DNA, Ribosomal Spacer/chemistry/genetics, DNA-Directed RNA Polymerases/genetics, Fatty Acids/analysis, Humans, Levofloxacin/pharmacology, Male, Microbial Sensitivity Tests, Moxifloxacin/pharmacology, Mycobacterium/*classification/genetics/*isolation & purification/physiology, Mycobacterium Infections, Nontuberculous/*microbiology, Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Sputum/*microbiologyPathogenicity

Reference

@idauthorscataloguedoi/urltitle
11605Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 44221)https://www.dsmz.de/collection/catalogue/details/culture/DSM-44221
20020Wink, J.http://www.dsmz.de/microorganisms/wink_pdf/DSM44221.pdfCompendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
36368Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/17407
53798Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 37671)https://www.ccug.se/strain?id=37671
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68379Automatically annotated from API Coryne
68382Automatically annotated from API zym
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
77862Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID41622.1StrainInfo: A central database for resolving microbial strain identifiers
121145Curators of the CIPCollection of Institut Pasteur (CIP 105393)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20105393