Strain identifier
BacDive ID: 8467
Type strain:
Species: Mycobacterium moriokaense
Strain Designation: 14 147 0001, 48504
Strain history: CIP <- 1998, V. Vincent, Inst. Pasteur, Paris, France: strain 14 147 0001 <- ATCC <- M. Tsukamura: strain NCH E11715 (= 48504)
NCBI tax ID(s): 39691 (species)
General
@ref: 11605
BacDive-ID: 8467
DSM-Number: 44221
keywords: genome sequence, 16S sequence, Bacteria, aerobe, mesophilic, human pathogen
description: Mycobacterium moriokaense 14 147 0001 is an aerobe, mesophilic human pathogen that was isolated from soil.
NCBI tax id
- NCBI tax id: 39691
- Matching level: species
strain history
@ref | history |
---|---|
11605 | <- M. Goodfellow, OI MOR <- JCM <- M. Tsukamura, NCH E11715 (=strain 48504) |
67770 | M. Tsukamura E11715 (=48504). |
121145 | CIP <- 1998, V. Vincent, Inst. Pasteur, Paris, France: strain 14 147 0001 <- ATCC <- M. Tsukamura: strain NCH E11715 (= 48504) |
doi: 10.13145/bacdive8467.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/actinomycetota
- domain: Bacteria
- phylum: Actinomycetota
- class: Actinomycetes
- order: Mycobacteriales
- family: Mycobacteriaceae
- genus: Mycobacterium
- species: Mycobacterium moriokaense
- full scientific name: Mycobacterium moriokaense Tsukamura et al. 1986
synonyms
- @ref: 20215
- synonym: Mycolicibacterium moriokaense
@ref: 11605
domain: Bacteria
phylum: Actinobacteria
class: Actinobacteria
order: Actinomycetales
family: Mycobacteriaceae
genus: Mycobacterium
species: Mycobacterium moriokaense
full scientific name: Mycobacterium moriokaense Tsukamura et al. 1986 emend. Nouioui et al. 2018
strain designation: 14 147 0001, 48504
type strain: yes
Morphology
cell morphology
@ref | motility | confidence | gram stain | cell shape |
---|---|---|---|---|
69480 | no | 91.04 | ||
69480 | 100 | positive | ||
121145 | no | rod-shaped |
colony morphology
@ref | colony color | incubation period | medium used |
---|---|---|---|
20020 | Oyster white (1013) | 10-14 days | 5006 |
20020 | Oyster white (1013) | MB7H10 | |
20020 | Oyster white (1013) | 10-14 days | MB7H11 |
53798 | 2 days |
Culture and growth conditions
culture medium
@ref | name | growth | link | composition |
---|---|---|---|---|
11605 | LÖWENSTEIN-JENSEN MEDIUM (DSMZ Medium 354) | yes | https://mediadive.dsmz.de/medium/354 | Name: LÖWENSTEIN-JENSEN MEDIUM (DSMZ Medium 354) Composition: Potato flour 18.6104 g/l L-Asparagin 2.23325 g/l KH2PO4 1.55087 g/l Mg-citrate 0.372208 g/l Malachite green 0.248139 g/l MgSO4 0.148883 g/l Glycerol Fresh egg mixture Distilled water |
11605 | MIDDLEBROOK MEDIUM (DSMZ Medium 645) | yes | https://mediadive.dsmz.de/medium/645 | Name: MIDDLEBROOK MEDIUM (DSMZ Medium 645) Composition: Bacto Middlebrook 7H10 agar 20.9945 g/l Glycerol Distilled water |
20020 | MB7H10 | yes | Name: Middlebrook 7H10 (MB7H10) Broth Base Composition: amount in percent Middlebrook 7H10 Agar 1.9% Glycerol 0.5% OADC-Enrichment in ml (NL5633) 10ml Comments: Add broth base and glycerol to ddh2O. Adjust volumes. Autoclave. Cool to 42°C. Add OADC-stock. Sterilisation: 20 minutes Temperature:121°C PH before: PH after: Biological details Culture type: suitable for: Mycobacteria OADC stock solution old broth-name: OADC-Stock Composition: Bovine Serum Albumin, fraction V 5 % 1% Oleic Acid in 0.2N NaOH 5ml Glucose 2 % NaCl 0.85 % Comments: Melt 1% Oleic Acid in 65°C water bath. Dissolve all ingredients in 700ml of ddH2O. Adjust volume to 1L. Filter sterilize and store at 4°C. -To make 1% Oleic Acid in 0.2N NaOH: Dissolve 5g oleic acid (ampule) in 500ml 0.2N NaOH (300ml ddH2O + 20ml NaOH + adjust volume to 500ml). Heat solution to 55°C for oleic acid to melt. Store at -20°C in 50ml aliquots. There is no need to sterilize this. Sterilisation: 20 minutes Temperature:121°C PH before: PH after: Biological details Culture type: suitable for: Mycobacteria | |
20020 | MB7H11 | yes | Name: Middlebrook 7H11 (MB7H11) Broth Base Composition: Middlebrook 7H11 Agar 2% Glycerol 0.5% OADC-Enrichment 10ml Comments: Add broth base and glycerol to ddH2O. Adjust volumes. Autoclave. Cool to 42°C. Add OADC-Stock. Sterilisation: 20 minutes Temperature:121°C PH before: PH after: Biological details Culture type: suitable for: Mycobacteria OADC stock solution old broth-name: OADC-Stock Composition: Bovine Serum Albumin, fraction V 5 % 1% Oleic Acid in 0.2N NaOH 5ml Glucose 2 % NaCl 0.85 % Comments: Melt 1% Oleic Acid in 65°C water bath. Dissolve all ingredients in 700ml of ddH2O. Adjust volume to 1L. Filter sterilize and store at 4°C. -To make 1% Oleic Acid in 0.2N NaOH: Dissolve 5g oleic acid (ampule) in 500ml 0.2N NaOH (300ml ddH2O + 20ml NaOH + adjust volume to 500ml). Heat solution to 55°C for oleic acid to melt. Store at -20°C in 50ml aliquots. There is no need to sterilize this. Sterilisation: 20 minutes Temperature:121°C PH before: PH after: Biological details Culture type: suitable for: Mycobacteria | |
20020 | 5006 | yes | Medium: 5006 Name: Composition (g/l) Sucrose 3,0 Dextrin 15,0 Meat extract 1,0 Yeast extract 2,0 Tryptone soy broth (Oxoid) 5,0 NaCl 0,5 K2HPO4 0,5 MgSO4 x 7 H2O 0,5 FeSO4 x 7 H2O 0,01 Agar 20,0 Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7,3 Usage: Maintenance Organisms: All Actinomycetes | |
36368 | MEDIUM 55 - for Mycobacterium | yes | ||
121145 | CIP Medium 55 | yes | https://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=55 |
culture temp
@ref | growth | type | temperature | range |
---|---|---|---|---|
20020 | positive | optimum | 37 | mesophilic |
11605 | positive | growth | 37 | mesophilic |
36368 | positive | growth | 37 | mesophilic |
53798 | positive | growth | 30-37 | mesophilic |
67770 | positive | growth | 37 | mesophilic |
121145 | positive | growth | 25-37 | mesophilic |
121145 | no | growth | 10 | psychrophilic |
121145 | no | growth | 45 | thermophilic |
Physiology and metabolism
oxygen tolerance
@ref | oxygen tolerance |
---|---|
53798 | aerobe |
121145 | obligate aerobe |
spore formation
- @ref: 69480
- spore formation: no
- confidence: 99.901
metabolite utilization
@ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
---|---|---|---|---|
121145 | 606565 | hippurate | - | hydrolysis |
121145 | 17632 | nitrate | + | reduction |
121145 | 16301 | nitrite | - | reduction |
121145 | 17632 | nitrate | - | respiration |
68379 | 4853 | esculin | - | hydrolysis |
68379 | 5291 | gelatin | - | hydrolysis |
68379 | 17634 | D-glucose | - | fermentation |
68379 | 16988 | D-ribose | - | fermentation |
68379 | 65327 | D-xylose | - | fermentation |
68379 | 16899 | D-mannitol | - | fermentation |
68379 | 17306 | maltose | - | fermentation |
68379 | 17716 | lactose | - | fermentation |
68379 | 28087 | glycogen | - | fermentation |
metabolite production
- @ref: 121145
- Chebi-ID: 35581
- metabolite: indole
- production: no
enzymes
@ref | value | activity | ec |
---|---|---|---|
68379 | gelatinase | - | |
68379 | beta-glucosidase | - | 3.2.1.21 |
68379 | N-acetyl-beta-glucosaminidase | - | 3.2.1.52 |
68379 | alpha-glucosidase | + | 3.2.1.20 |
68379 | beta-galactosidase | - | 3.2.1.23 |
68379 | beta-glucuronidase | - | 3.2.1.31 |
68379 | alkaline phosphatase | - | 3.1.3.1 |
121145 | oxidase | - | |
121145 | beta-galactosidase | - | 3.2.1.23 |
121145 | gelatinase | - | |
121145 | amylase | - | |
121145 | DNase | - | |
121145 | caseinase | - | 3.4.21.50 |
121145 | catalase | + | 1.11.1.6 |
121145 | tween esterase | - | |
121145 | gamma-glutamyltransferase | - | 2.3.2.2 |
121145 | lecithinase | - | |
121145 | lipase | - | |
121145 | urease | + | 3.5.1.5 |
68382 | alkaline phosphatase | - | 3.1.3.1 |
68382 | esterase lipase (C 8) | + | |
68382 | lipase (C 14) | - | |
68382 | leucine arylamidase | + | 3.4.11.1 |
68382 | alpha-galactosidase | - | 3.2.1.22 |
68382 | beta-galactosidase | - | 3.2.1.23 |
68382 | beta-glucuronidase | - | 3.2.1.31 |
68382 | N-acetyl-beta-glucosaminidase | - | 3.2.1.52 |
68382 | alpha-fucosidase | - | 3.2.1.51 |
API coryne
@ref | NIT | PYZ | PYRA | PAL | beta GUR | beta GAL | alpha GLU | beta NAG | ESC | URE | GEL | GLU | RIB | XYL | MAN | MAL | LAC | SAC | GLYG | Control |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
20020 | - | + | + | - | - | - | + | - | - | + | - | - | - | - | - | - | - | + | - | |
11605 | + | +/- | - | - | - | - | + | - | - | - | - | - | - | - | - | - | - | - | - | - |
API zym
@ref | Alkaline phosphatase | Esterase | Esterase Lipase | Lipase | Leucine arylamidase | Valine arylamidase | Cystine arylamidase | Trypsin | alpha- Chymotrypsin | Acid phosphatase | Naphthol-AS-BI-phosphohydrolase | alpha- Galactosidase | beta- Galactosidase | beta- Glucuronidase | alpha- Glucosidase | beta- Glucosidase | N-acetyl-beta- glucosaminidase | alpha- Mannosidase | alpha- Fucosidase | Control |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
20020 | - | + | + | - | + | + | + | + | + | + | - | - | - | - | + | + | - | + | - | |
11605 | - | +/- | + | - | + | - | +/- | +/- | - | - | - | - | - | - | - | - | - | - | - | - |
121145 | - | + | + | - | + | - | - | - | - | + | + | - | - | - | - | - | - | - | - | - |
API biotype100
@ref | GLU | FRU | GAL | TRE | MNE | SBE | MEL | SAC | RAF | MTE | MAL | LAC | LTE | MbGa | MaGa | CEL | GEN | MbGu | ESC | RIB | ARA | XYL | PLE | RHA | FUC | MLZ | DARL | LARL | XLT | DUL | TAG | GLY | INO | MAN | MTL | TUR | SOR | ADO | HBG | LYX | ERY | MDG | 3MDG | SAT | MUC | LTAT | DTAT | MTAT | DMLT | LMLT | CATE | TATE | TTE | CIT | GRT | GAT | 2KG | 5KG | TRY | NAG | GNT | PAC | PAT | pOBE | QAT | GTE | mOBE | BAT | PPAT | CMT | TGE | BET | PCE | ABT | HIN | LAT | CAP | CYT | HIS | SUC | FUM | GRE | GYT | AVT | ETN | TTN | GLN | ITA | 3OBU | APT | GTT | PRO | DALA | LALA | SER | MNT | PROP | TYR | 2KT |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
121145 | + | - | - | - | - | - | - | - | - | - | - | - | - | - | + | - | - | - | - | - | - | - | - | - | - | - | + | - | - | - | - | - | - | + | - | - | + | - | - | - | - | - | - | - | - | - | - | - | - | + | - | - | - | - | - | - | - | - | - | - | + | - | - | - | - | - | - | - | - | - | - | - | + | - | - | + | - | - | + | - | - | - | - | - | - | - | - | - | + | - | - | - | - | - | - | - | + | - | - |
Isolation, sampling and environmental information
isolation
@ref | sample type | country | origin.country | continent |
---|---|---|---|---|
11605 | soil | Japan | JPN | Asia |
53798 | Soil | |||
67770 | Soil | |||
121145 | Environment, Soil | Japan | JPN | Asia |
isolation source categories
- Cat1: #Environmental
- Cat2: #Terrestrial
- Cat3: #Soil
Safety information
risk assessment
@ref | pathogenicity human | biosafety level | biosafety level comment |
---|---|---|---|
11605 | yes, in single cases | 1 | Risk group (German classification) |
20020 | 1 | German classification | |
121145 | 1 | Risk group (French classification) |
Sequence information
16S sequences
- @ref: 11605
- description: Mycobacterium moriokaense 16S rRNA gene, strain DSM 44221T
- accession: AJ429044
- length: 1493
- database: ena
- NCBI tax ID: 39691
Genome sequences
@ref | description | accession | assembly level | database | NCBI tax ID |
---|---|---|---|---|---|
66792 | Mycolicibacterium moriokaense JCM 6375 | GCA_010726085 | complete | ncbi | 39691 |
66792 | Mycolicibacterium moriokaense DSM 44221 | GCA_003347205 | contig | ncbi | 39691 |
66792 | Mycobacterium moriokaense strain CIP105393 | 39691.4 | wgs | patric | 39691 |
66792 | Mycobacterium moriokaense strain CIP105393 | 39691.3 | wgs | patric | 39691 |
66792 | Mycolicibacterium moriokaense strain DSM 44221 | 39691.9 | wgs | patric | 39691 |
66792 | Mycolicibacterium moriokaense strain JCM 6375 | 39691.13 | complete | patric | 39691 |
66792 | Mycolicibacterium moriokaense CIP105393 | 2865562250 | draft | img | 39691 |
66792 | Mycolicibacterium moriokaense DSM 44221 | 2856244778 | draft | img | 39691 |
67770 | Mycolicibacterium moriokaense CIP105393 | GCA_002086395 | contig | ncbi | 39691 |
66792 | Mycolicibacterium moriokaense DSM 44221 | GCA_025822185 | contig | ncbi | 39691 |
GC content
@ref | GC-content | method |
---|---|---|
67770 | 64 | thermal denaturation, midpoint method (Tm) |
67770 | 66 | genome sequence analysis |
Genome-based predictions
predictions
trait | prediction | confidence | training_data |
---|---|---|---|
spore-forming | no | 86 | no |
motile | no | 93.696 | no |
flagellated | no | 98.496 | no |
gram-positive | yes | 90.008 | no |
anaerobic | no | 99.282 | yes |
aerobic | yes | 92.629 | yes |
halophile | no | 93.962 | no |
spore-forming | no | 87.971 | no |
glucose-util | yes | 82.544 | no |
thermophile | no | 99.567 | no |
glucose-ferment | no | 89.383 | yes |
External links
@ref: 11605
culture collection no.: DSM 44221, ATCC 43059, JCM 6375, CCUG 37671, CIP 105393, KCTC 9516, VKM Ac-1183, NCH E11715
straininfo link
- @ref: 77862
- straininfo: 41622
literature
topic | Pubmed-ID | title | authors | journal | DOI | year | mesh | topic2 |
---|---|---|---|---|---|---|---|---|
Phylogeny | 30696899 | Genome-guided and mass spectrometry investigation of natural products produced by a potential new actinobacterial strain isolated from a mangrove ecosystem in Futian, Shenzhen, China. | Hu D, Gao C, Sun C, Jin T, Fan G, Mok KM, Lee SM | Sci Rep | 10.1038/s41598-018-37475-w | 2019 | Anti-Bacterial Agents/isolation & purification/metabolism/*pharmacology, Bacterial Typing Techniques, Biological Products/isolation & purification/metabolism/*pharmacology, China, DNA, Bacterial/genetics, Genome, Bacterial/*genetics, Mycobacterium/classification/*genetics/isolation & purification/*metabolism, RNA, Ribosomal, 16S/genetics, Secondary Metabolism/physiology, Sequence Analysis, DNA, Soil Microbiology, Wetlands, Whole Genome Sequencing | Metabolism |
Phylogeny | 31460855 | Mycolicibacterium stellerae sp. nov., a rapidly growing scotochromogenic strain isolated from Stellera chamaejasme. | Nouioui I, Sangal V, Cortes-Albayay C, Jando M, Igual JM, Klenk HP, Zhang YQ, Goodfellow M | Int J Syst Evol Microbiol | 10.1099/ijsem.0.003644 | 2019 | Bacterial Typing Techniques, Base Composition, China, DNA, Bacterial/genetics, Fatty Acids/chemistry, Mycobacteriaceae/*classification/isolation & purification, Nucleic Acid Hybridization, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Thymelaeaceae/*microbiology | Transcriptome |
Phylogeny | 32054545 | Phenotypic and molecular characterisation of a novel species, Mycobacterium hubeiense sp., isolated from the sputum of a patient with secondary tuberculosis in Hubei of China. | Yu X, Zheng H, Zhou F, Hu P, Wang H, Li N, He J, Wang P, Zhang L, Men H, Xiang J, Zhang S | Epidemiol Infect | 10.1017/S0950268820000436 | 2020 | Aged, 80 and over, Antitubercular Agents/pharmacology, Bacterial Proteins/genetics, Base Composition, Chaperonin 60/genetics, China, Cluster Analysis, Cytosol/chemistry, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, DNA, Ribosomal Spacer/chemistry/genetics, DNA-Directed RNA Polymerases/genetics, Fatty Acids/analysis, Humans, Levofloxacin/pharmacology, Male, Microbial Sensitivity Tests, Moxifloxacin/pharmacology, Mycobacterium/*classification/genetics/*isolation & purification/physiology, Mycobacterium Infections, Nontuberculous/*microbiology, Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Sputum/*microbiology | Pathogenicity |
Reference
@id | authors | catalogue | doi/url | title |
---|---|---|---|---|
11605 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 44221) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-44221 | |
20020 | Wink, J. | http://www.dsmz.de/microorganisms/wink_pdf/DSM44221.pdf | Compendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig | |
20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |
36368 | Curators of the CIP | https://brclims.pasteur.fr/brcWeb/souche/detail/1/17407 | ||
53798 | Curators of the CCUG | Culture Collection University of Gothenburg (CCUG) (CCUG 37671) | https://www.ccug.se/strain?id=37671 | |
66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |
67770 | Curators of the JCM | https://jcm.brc.riken.jp/en/ | ||
68379 | Automatically annotated from API Coryne | |||
68382 | Automatically annotated from API zym | |||
69480 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |
77862 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID41622.1 | StrainInfo: A central database for resolving microbial strain identifiers | |
121145 | Curators of the CIP | Collection of Institut Pasteur (CIP 105393) | https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20105393 |