Strain identifier

BacDive ID: 8463

Type strain: Yes

Species: Mycobacterium hassiacum

Strain Designation: 3849

Strain history: CIP <- 1997, U. Reischl and L. Naumann, Regensburg Univ., Regensburg, Germany: strain 3849 <- Hess

NCBI tax ID(s): 1122247 (strain), 46351 (species)

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General

@ref: 11595

BacDive-ID: 8463

DSM-Number: 44199

keywords: genome sequence, 16S sequence, Bacteria, aerobe, mesophilic

description: Mycobacterium hassiacum 3849 is an aerobe, mesophilic bacterium that was isolated from urine.

NCBI tax id

NCBI tax idMatching level
46351species
1122247strain

strain history

@refhistory
11595<- L. Naumann, Universität Regensburg <- Labor Dr. Hess, Kassel
67770CIP 105218 <-- U. Reischl and L. Naumann 3849 <-- L. Hess.
119847CIP <- 1997, U. Reischl and L. Naumann, Regensburg Univ., Regensburg, Germany: strain 3849 <- Hess

doi: 10.13145/bacdive8463.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Mycobacteriales
  • family: Mycobacteriaceae
  • genus: Mycobacterium
  • species: Mycobacterium hassiacum
  • full scientific name: Mycobacterium hassiacum Schröder et al. 1997

  • synonyms

    • @ref: 20215
    • synonym: Mycolicibacterium hassiacum

@ref: 11595

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Mycobacteriaceae

genus: Mycobacterium

species: Mycobacterium hassiacum

full scientific name: Mycobacterium hassiacum Schröder et al. 1997 emend. Nouioui et al. 2018

strain designation: 3849

type strain: yes

Morphology

cell morphology

@refmotilityconfidencegram staincell shape
69480no93.935
69480100positive
119847rod-shaped

colony morphology

@refcolony colorincubation periodmedium used
19849Rape yellow10-14 days5006
19849Rape yellow10-14 daysMB7H10
19849Rape yellow10-14 daysMB7H11

multimedia

@refmultimedia contentintellectual property rightscaption
11595https://www.dsmz.de/microorganisms/photos/DSM_44199-1.jpg© Leibniz-Institut DSMZ
11595https://www.dsmz.de/microorganisms/photos/DSM_44199.jpg© Leibniz-Institut DSMZMedium 645 37°C

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
11595MIDDLEBROOK MEDIUM (DSMZ Medium 645)yeshttps://mediadive.dsmz.de/medium/645Name: MIDDLEBROOK MEDIUM (DSMZ Medium 645) Composition: Bacto Middlebrook 7H10 agar 20.9945 g/l Glycerol Distilled water
19849MB7H10yesName: Middlebrook 7H10 (MB7H10) Broth Base Composition: amount in percent Middlebrook 7H10 Agar 1.9% Glycerol 0.5% OADC-Enrichment in ml (NL5633) 10ml Comments: Add broth base and glycerol to ddh2O. Adjust volumes. Autoclave. Cool to 42°C. Add OADC-stock. Sterilisation: 20 minutes Temperature:121°C PH before: PH after: Biological details Culture type: suitable for: Mycobacteria OADC stock solution old broth-name: OADC-Stock Composition: Bovine Serum Albumin, fraction V 5 % 1% Oleic Acid in 0.2N NaOH 5ml Glucose 2 % NaCl 0.85 % Comments: Melt 1% Oleic Acid in 65°C water bath. Dissolve all ingredients in 700ml of ddH2O. Adjust volume to 1L. Filter sterilize and store at 4°C. -To make 1% Oleic Acid in 0.2N NaOH: Dissolve 5g oleic acid (ampule) in 500ml 0.2N NaOH (300ml ddH2O + 20ml NaOH + adjust volume to 500ml). Heat solution to 55°C for oleic acid to melt. Store at -20°C in 50ml aliquots. There is no need to sterilize this. Sterilisation: 20 minutes Temperature:121°C PH before: PH after: Biological details Culture type: suitable for: Mycobacteria
19849MB7H11yesName: Middlebrook 7H11 (MB7H11) Broth Base Composition: Middlebrook 7H11 Agar 2% Glycerol 0.5% OADC-Enrichment 10ml Comments: Add broth base and glycerol to ddH2O. Adjust volumes. Autoclave. Cool to 42°C. Add OADC-Stock. Sterilisation: 20 minutes Temperature:121°C PH before: PH after: Biological details Culture type: suitable for: Mycobacteria OADC stock solution old broth-name: OADC-Stock Composition: Bovine Serum Albumin, fraction V 5 % 1% Oleic Acid in 0.2N NaOH 5ml Glucose 2 % NaCl 0.85 % Comments: Melt 1% Oleic Acid in 65°C water bath. Dissolve all ingredients in 700ml of ddH2O. Adjust volume to 1L. Filter sterilize and store at 4°C. -To make 1% Oleic Acid in 0.2N NaOH: Dissolve 5g oleic acid (ampule) in 500ml 0.2N NaOH (300ml ddH2O + 20ml NaOH + adjust volume to 500ml). Heat solution to 55°C for oleic acid to melt. Store at -20°C in 50ml aliquots. There is no need to sterilize this. Sterilisation: 20 minutes Temperature:121°C PH before: PH after: Biological details Culture type: suitable for: Mycobacteria
198495006yesMedium: 5006 Name: Composition (g/l) Sucrose 3,0 Dextrin 15,0 Meat extract 1,0 Yeast extract 2,0 Tryptone soy broth (Oxoid) 5,0 NaCl 0,5 K2HPO4 0,5 MgSO4 x 7 H2O 0,5 FeSO4 x 7 H2O 0,01 Agar 20,0 Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7,3 Usage: Maintenance Organisms: All Actinomycetes
36331MEDIUM 55 - for Mycobacteriumyes
119847CIP Medium 55yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=55

culture temp

@refgrowthtypetemperaturerange
19849positiveoptimum37mesophilic
11595positivegrowth37mesophilic
36331positivegrowth37mesophilic
53712positivegrowth37-55
67770positivegrowth37mesophilic
119847positivegrowth30-45
119847nogrowth10psychrophilic
119847nogrowth22psychrophilic

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
53712aerobe
119847obligate aerobe

spore formation

  • @ref: 69480
  • spore formation: no
  • confidence: 99.915

observation

  • @ref: 67770
  • observation: quinones: MK-9(H2)

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
119847606565hippurate-hydrolysis
11984717632nitrate+reduction
11984716301nitrite-reduction
11984717632nitrate-respiration
6837917632nitrate-reduction
683794853esculin-hydrolysis
6837916199urea-hydrolysis
683795291gelatin-hydrolysis
6837917634D-glucose-fermentation
6837916988D-ribose-fermentation
6837965327D-xylose-fermentation
6837916899D-mannitol-fermentation
6837917306maltose-fermentation
6837917716lactose-fermentation
6837917992sucrose-fermentation
6837928087glycogen-fermentation

metabolite production

  • @ref: 119847
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

enzymes

@refvalueactivityec
68379gelatinase-
68379urease-3.5.1.5
68379beta-glucosidase-3.2.1.21
68379N-acetyl-beta-glucosaminidase-3.2.1.52
68379alpha-glucosidase-3.2.1.20
68379beta-galactosidase-3.2.1.23
68379beta-glucuronidase-3.2.1.31
68379alkaline phosphatase+3.1.3.1
68379pyrrolidonyl arylamidase+3.4.19.3
68379pyrazinamidase+3.5.1.B15
119847oxidase-
119847beta-galactosidase-3.2.1.23
119847catalase+1.11.1.6
119847gamma-glutamyltransferase-2.3.2.2
119847urease+3.5.1.5
68382esterase (C 4)+
68382esterase lipase (C 8)+
68382leucine arylamidase+3.4.11.1
68382valine arylamidase+
68382cystine arylamidase+3.4.11.3
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase-3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase-3.2.1.20
68382beta-glucosidase-3.2.1.21
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-fucosidase-3.2.1.51

API coryne

@refNITPYZPYRAPALbeta GURbeta GALalpha GLUbeta NAGESCUREGELGLURIBXYLMANMALLACSACGLYG
19849-+++---------------

API zym

@refAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- FucosidaseControl
19849++++++++-++------+-
119847-++-+++--++---------

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentisolation date
11595urineHessenGermanyDEUEurope
53712Human urine
67770Urine
119847UrineHessenGermanyDEUEurope1995

isolation source categories

  • Cat1: #Host Body Product
  • Cat2: #Fluids
  • Cat3: #Urine

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
115951Risk group (German classification)
198491Risk group (German classification)
1198471Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Mycobacterium hassiacum 16S ribosomal RNA gene, partial sequenceU494011491ena46351
20218Mycobacterium hassiacum strain CIP 105218 16S ribosomal RNA gene, partial sequenceAF547933547ena46351

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Mycolicibacterium hassiacum DSM 44199 MhassiacumGCA_900603025completencbi1122247
66792Mycobacterium hassiacum DSM 441991122247.3wgspatric1122247
66792Mycobacterium hassiacum DSM 441991122247.4wgspatric1122247
66792Mycolicibacterium hassiacum DSM 44199 strain Mhassiacum1122247.5completepatric1122247
66792Mycolicibacterium hassiacum DSM 441992531839488draftimg1122247
66792Mycolicibacterium hassiacum DSM 441992515154012draftimg1122247
66792Mycolicibacterium hassiacum DSM 441992856852935completeimg1122247
67770Mycolicibacterium hassiacum DSM 44199GCA_000300375contigncbi1122247
67770Mycolicibacterium hassiacum DSM 44199GCA_000379865scaffoldncbi1122247
66792Mycolicibacterium hassiacum DSM 44199GCA_027886095contigncbi1122247

GC content

@refGC-contentmethod
6777069.4genome sequence analysis
6777065.8high performance liquid chromatography (HPLC)

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno81no
flagellatedno98.887no
gram-positiveyes88.575no
anaerobicno98.973no
aerobicyes90.023yes
halophileno84.501no
spore-formingno88.53no
thermophileno98.407yes
glucose-utilyes82.491no
motileno95.609no
glucose-fermentno92.377yes

External links

@ref: 11595

culture collection no.: DSM 44199, CCUG 37519, CIP 105218, JCM 12690

straininfo link

  • @ref: 77858
  • straininfo: 50521

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Genetics23209251Genome sequence of Mycobacterium hassiacum DSM 44199, a rare source of heat-stable mycobacterial proteins.Tiago I, Maranha A, Mendes V, Alarico S, Moynihan PJ, Clarke AJ, Macedo-Ribeiro S, Pereira PJ, Empadinhas NJ Bacteriol10.1128/JB.01880-122012*Bacterial Proteins/metabolism, Base Composition, Base Sequence, DNA, Bacterial/genetics, *Genome, Bacterial, Hot Temperature, Humans, Molecular Sequence Data, Nontuberculous Mycobacteria/classification/*genetics/isolation & purification, Phylogeny, Protein Stability, RNA, Bacterial/genetics, Sequence Analysis, DNA, Urine/microbiologyStress
Genetics30701245Complete Genome Sequence of Mycolicibacterium hassiacum DSM 44199.Sanchez M, Blesa A, Sacristan-Horcajada E, Berenguer JMicrobiol Resour Announc10.1128/MRA.01522-182019

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
11595Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 44199)https://www.dsmz.de/collection/catalogue/details/culture/DSM-44199
19849Wink, J.http://www.dsmz.de/microorganisms/wink_pdf/DSM44199.pdfCompendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
36331Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/17214
53712Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 37519)https://www.ccug.se/strain?id=37519
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
66794Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg10.1093/nar/gkaa1025BRENDA, the ELIXIR core data resource in 2021: new developments and updatesNucleic Acids Res. 49: D498-D508 202033211880
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68379Automatically annotated from API Coryne
68382Automatically annotated from API zym
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
77858Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID50521.1StrainInfo: A central database for resolving microbial strain identifiers
119847Curators of the CIPCollection of Institut Pasteur (CIP 105218)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20105218