Strain identifier
BacDive ID: 8446
Type strain:
Species: Mycobacterium brumae
Strain Designation: CR-270, 14 401 0001
Strain history: CIP <- 1993, V. Vincent, Inst. Pasteur, Paris, France: strain 14 401 0001 <- A. Ausina, Dept. Microbiol., Med. Univ., Barcelona, Spain: strain CR-270
NCBI tax ID(s): 1335619 (strain), 85968 (species)
General
@ref: 11575
BacDive-ID: 8446
DSM-Number: 44177
keywords: genome sequence, 16S sequence, Bacteria, spore-forming, mesophilic
description: Mycobacterium brumae CR-270 is a spore-forming, mesophilic bacterium that was isolated from water sample.
NCBI tax id
NCBI tax id | Matching level |
---|---|
85968 | species |
1335619 | strain |
strain history
@ref | history |
---|---|
11575 | <- CIP <- V. Vincent, Inst. Pasteur Paris <- A. Ausina, Dept. Microbiol., Med. Univ., Barcelona, CR-270 |
67770 | CECT 3022 <-- V. Ausina and M. Luquin CR-270. |
117061 | CIP <- 1993, V. Vincent, Inst. Pasteur, Paris, France: strain 14 401 0001 <- A. Ausina, Dept. Microbiol., Med. Univ., Barcelona, Spain: strain CR-270 |
doi: 10.13145/bacdive8446.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/actinomycetota
- domain: Bacteria
- phylum: Actinomycetota
- class: Actinomycetes
- order: Mycobacteriales
- family: Mycobacteriaceae
- genus: Mycobacterium
- species: Mycobacterium brumae
- full scientific name: Mycobacterium brumae Luquin et al. 1993
synonyms
- @ref: 20215
- synonym: Mycolicibacterium brumae
@ref: 11575
domain: Bacteria
phylum: Actinobacteria
class: Actinobacteria
order: Actinomycetales
family: Mycobacteriaceae
genus: Mycobacterium
species: Mycobacterium brumae
full scientific name: Mycobacterium brumae Luquin et al. 1993
strain designation: CR-270, 14 401 0001
type strain: yes
Morphology
cell morphology
@ref | motility | confidence | gram stain | cell shape |
---|---|---|---|---|
69480 | no | 92.591 | ||
69480 | 100 | positive | ||
117061 | no | rod-shaped |
colony morphology
@ref | colony color | incubation period | medium used |
---|---|---|---|
20012 | Cream (9001) | 10-14 days | 5006 |
20012 | Cream (9001) | 10-14 days | BHI |
20012 | Cream (9001) | 10-14 days | BUG |
20012 | Cream (9001) | 10-14 days | MB7H9 |
multicellular morphology
@ref | forms multicellular complex | medium name |
---|---|---|
20012 | no | ISP 2 |
20012 | no | ISP 3 |
20012 | no | ISP 4 |
20012 | no | ISP 5 |
20012 | no | ISP 6 |
20012 | no | ISP 7 |
multimedia
- @ref: 11575
- multimedia content: https://www.dsmz.de/microorganisms/photos/DSM_44177.jpg
- caption: Medium 645 37°C
- intellectual property rights: © Leibniz-Institut DSMZ
Culture and growth conditions
culture medium
@ref | name | growth | link | composition |
---|---|---|---|---|
11575 | LÖWENSTEIN-JENSEN MEDIUM (DSMZ Medium 354) | yes | https://mediadive.dsmz.de/medium/354 | Name: LÖWENSTEIN-JENSEN MEDIUM (DSMZ Medium 354) Composition: Potato flour 18.6104 g/l L-Asparagin 2.23325 g/l KH2PO4 1.55087 g/l Mg-citrate 0.372208 g/l Malachite green 0.248139 g/l MgSO4 0.148883 g/l Glycerol Fresh egg mixture Distilled water |
11575 | MIDDLEBROOK MEDIUM (DSMZ Medium 645) | yes | https://mediadive.dsmz.de/medium/645 | Name: MIDDLEBROOK MEDIUM (DSMZ Medium 645) Composition: Bacto Middlebrook 7H10 agar 20.9945 g/l Glycerol Distilled water |
20012 | BUG | yes | ||
20012 | BHI | yes | ||
20012 | 5006 | yes | Medium: 5006 Name: Composition (g/l) Sucrose 3,0 Dextrin 15,0 Meat extract 1,0 Yeast extract 2,0 Tryptone soy broth (Oxoid) 5,0 NaCl 0,5 K2HPO4 0,5 MgSO4 x 7 H2O 0,5 FeSO4 x 7 H2O 0,01 Agar 20,0 Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7,3 Usage: Maintenance Organisms: All Actinomycetes | |
20012 | MB7H9 | yes | Name: Middlebrook 7H9 (MB7H9) Broth Base Composition: Middelbrook 7H9 Broth Base 4.7 g/l Glycerol 2.0 g/l Casaminoacids 11.0 g/l Yeast extract 5.5 g/l Tween80 (20%) 2.5 g/l OADC-Enrichment 100 ml Agar 15.0 g/l Preparation: Dissolve in Milli-Q-H2O. Before Use add 10% OADC stock solution Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Maintenance and revitalization Organisms: Mycobacteria OADC stock solution old broth-name: OADC-Stock Composition: Bovine Serum Albumin, fraction V 5 % 1% Oleic Acid in 0.2N NaOH 5ml Glucose 2 % NaCl 0.85 % Comments: Melt 1% Oleic Acid in 65°C water bath. Dissolve all ingredients in 700ml of ddH2O. Adjust volume to 1L. Filter sterilize and store at 4°C. -To make 1% Oleic Acid in 0.2N NaOH: Dissolve 5g oleic acid (ampule) in 500ml 0.2N NaOH (300ml ddH2O + 20ml NaOH + adjust volume to 500ml). Heat solution to 55°C for oleic acid to melt. Store at -20°C in 50ml aliquots. There is no need to sterilize this. Sterilisation: 20 minutes Temperature:121°C PH before: PH after: Biological details Culture type: suitable for: Mycobacteria | |
40657 | MEDIUM 55 - for Mycobacterium | yes | ||
117061 | CIP Medium 55 | yes | https://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=55 |
culture temp
@ref | growth | type | temperature | range |
---|---|---|---|---|
20012 | positive | optimum | 37 | mesophilic |
11575 | positive | growth | 37 | mesophilic |
40657 | positive | growth | 30 | mesophilic |
67770 | positive | growth | 30 | mesophilic |
117061 | positive | growth | 30-37 | mesophilic |
Physiology and metabolism
spore formation
@ref | spore description | spore formation | confidence |
---|---|---|---|
20012 | Formation of sporangia (multiocular), spore surface smooth | yes | |
69481 | no | 94 | |
69480 | no | 99.975 |
metabolite utilization
@ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
---|---|---|---|---|
117061 | 16947 | citrate | - | carbon source |
117061 | 17632 | nitrate | + | reduction |
68379 | 17632 | nitrate | - | reduction |
68379 | 4853 | esculin | - | hydrolysis |
68379 | 16199 | urea | + | hydrolysis |
68379 | 5291 | gelatin | - | hydrolysis |
68379 | 17634 | D-glucose | - | fermentation |
68379 | 16988 | D-ribose | - | fermentation |
68379 | 65327 | D-xylose | - | fermentation |
68379 | 16899 | D-mannitol | - | fermentation |
68379 | 17306 | maltose | - | fermentation |
68379 | 17716 | lactose | - | fermentation |
68379 | 17992 | sucrose | - | fermentation |
68379 | 28087 | glycogen | - | fermentation |
metabolite production
- @ref: 117061
- Chebi-ID: 35581
- metabolite: indole
- production: no
enzymes
@ref | value | activity | ec |
---|---|---|---|
68379 | gelatinase | - | |
68379 | urease | + | 3.5.1.5 |
68379 | beta-glucosidase | - | 3.2.1.21 |
68379 | N-acetyl-beta-glucosaminidase | - | 3.2.1.52 |
68379 | alpha-glucosidase | - | 3.2.1.20 |
68379 | beta-galactosidase | - | 3.2.1.23 |
68379 | beta-glucuronidase | - | 3.2.1.31 |
68379 | alkaline phosphatase | - | 3.1.3.1 |
68379 | pyrrolidonyl arylamidase | - | 3.4.19.3 |
68379 | pyrazinamidase | + | 3.5.1.B15 |
117061 | oxidase | - | |
117061 | catalase | + | 1.11.1.6 |
117061 | tween esterase | + | |
117061 | urease | + | 3.5.1.5 |
68382 | alkaline phosphatase | - | 3.1.3.1 |
68382 | esterase (C 4) | + | |
68382 | esterase lipase (C 8) | + | |
68382 | lipase (C 14) | - | |
68382 | leucine arylamidase | + | 3.4.11.1 |
68382 | valine arylamidase | - | |
68382 | cystine arylamidase | - | 3.4.11.3 |
68382 | trypsin | - | 3.4.21.4 |
68382 | alpha-chymotrypsin | - | 3.4.21.1 |
68382 | acid phosphatase | - | 3.1.3.2 |
68382 | naphthol-AS-BI-phosphohydrolase | - | |
68382 | alpha-galactosidase | - | 3.2.1.22 |
68382 | beta-galactosidase | - | 3.2.1.23 |
68382 | beta-glucuronidase | - | 3.2.1.31 |
68382 | alpha-glucosidase | - | 3.2.1.20 |
68382 | beta-glucosidase | - | 3.2.1.21 |
68382 | N-acetyl-beta-glucosaminidase | - | 3.2.1.52 |
68382 | alpha-mannosidase | - | 3.2.1.24 |
68382 | alpha-fucosidase | - | 3.2.1.51 |
API coryne
@ref | NIT | PYZ | PYRA | PAL | beta GUR | beta GAL | alpha GLU | beta NAG | ESC | URE | GEL | GLU | RIB | XYL | MAN | MAL | LAC | SAC | GLYG |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
20012 | - | + | - | - | - | - | - | - | - | + | - | - | - | - | - | - | - | - | - |
API zym
@ref | Alkaline phosphatase | Esterase | Esterase Lipase | Lipase | Leucine arylamidase | Valine arylamidase | Cystine arylamidase | Trypsin | alpha- Chymotrypsin | Acid phosphatase | Naphthol-AS-BI-phosphohydrolase | alpha- Galactosidase | beta- Galactosidase | beta- Glucuronidase | alpha- Glucosidase | beta- Glucosidase | N-acetyl-beta- glucosaminidase | alpha- Mannosidase | alpha- Fucosidase |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
20012 | - | + | + | - | + | - | - | - | - | - | - | - | - | - | - | - | - | - | - |
API biotype100
@ref | GLU | FRU | GAL | TRE | MNE | SBE | MEL | SAC | RAF | MTE | MAL | LAC | LTE | MbGa | MaGa | CEL | GEN | MbGu | ESC | RIB | ARA | XYL | PLE | RHA | FUC | MLZ | DARL | LARL | XLT | DUL | TAG | GLY | INO | MAN | MTL | TUR | SOR | ADO | HBG | LYX | ERY | MDG | 3MDG | SAT | MUC | LTAT | DTAT | MTAT | DMLT | LMLT | CATE | TATE | TTE | CIT | GRT | GAT | 2KG | 5KG | TRY | NAG | GNT | PAC | PAT | pOBE | QAT | GTE | mOBE | BAT | PPAT | CMT | TGE | BET | PCE | ABT | HIN | LAT | CAP | CYT | HIS | SUC | FUM | GRE | GYT | AVT | ETN | TTN | GLN | ITA | 3OBU | APT | GTT | PRO | DALA | LALA | SER | MNT | PROP | TYR | 2KT |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
117061 | + | + | - | - | - | - | - | + | - | - | + | - | - | - | - | - | - | - | - | + | - | - | - | - | - | - | - | - | - | - | - | - | + | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | + | + | - | - | - | - | - | - | - | - | - | - | - | + | - | - | - | - | - | + | - | - | - | - | + | - | - | + | - | - | - | + | - | - | - | - | - | - | - | - |
Isolation, sampling and environmental information
isolation
@ref | sample type | geographic location | country | origin.country | continent |
---|---|---|---|---|---|
11575 | water sample | Barcelona | Spain | ESP | Europe |
53751 | Water | Barcelona | Spain | ESP | Europe |
67770 | Water from the Llobregat River | Barcelona | Spain | ESP | Europe |
117061 | Environment, Water sample | Barcelona | Spain | ESP | Europe |
isolation source categories
- Cat1: #Environmental
- Cat2: #Aquatic
Safety information
risk assessment
@ref | biosafety level | biosafety level comment |
---|---|---|
11575 | 1 | Risk group (German classification) |
20012 | 1 | German classification |
117061 | 1 | Risk group (French classification) |
Sequence information
16S sequences
@ref | description | accession | length | database | NCBI tax ID |
---|---|---|---|---|---|
20218 | Mycobacterium brumae 16S ribosomal RNA gene, partial sequence | AF480576 | 1449 | ena | 85968 |
20218 | Mycobacterium brumae strain CIP 103465 16S ribosomal RNA gene, partial sequence | AF547907 | 539 | ena | 85968 |
Genome sequences
@ref | description | accession | assembly level | database | NCBI tax ID |
---|---|---|---|---|---|
66792 | Mycolicibacterium brumae DSM 44177 | 1335619.3 | wgs | patric | 1335619 |
66792 | Mycolicibacterium brumae DSM 44177 | 2866967103 | draft | img | 1335619 |
67770 | Mycolicibacterium brumae DSM 44177 | GCA_004014795 | contig | ncbi | 1335619 |
66792 | Mycolicibacterium brumae ATCC 51384 | GCA_025215495 | complete | ncbi | 85968 |
66792 | Mycolicibacterium brumae DSM 44177 | GCA_025822525 | scaffold | ncbi | 85968 |
66792 | Mycolicibacterium brumae strain ATCC 51384 | 85968.9 | complete | patric | 85968 |
Genome-based predictions
predictions
trait | prediction | confidence | training_data |
---|---|---|---|
spore-forming | no | 94 | no |
motile | no | 95.898 | no |
flagellated | no | 98.037 | no |
gram-positive | yes | 88.492 | no |
anaerobic | no | 99.129 | no |
aerobic | yes | 81.608 | no |
halophile | no | 92.385 | no |
spore-forming | no | 88.426 | yes |
glucose-ferment | no | 89.477 | no |
thermophile | no | 97.711 | yes |
glucose-util | yes | 86.33 | no |
External links
@ref: 11575
culture collection no.: DSM 44177, ATCC 51384, CIP 103465, CCUG 37586, JCM 12273, CECT 3022, KCTC 19711
straininfo link
- @ref: 77844
- straininfo: 46328
literature
topic | Pubmed-ID | title | authors | journal | DOI | year | mesh | topic2 |
---|---|---|---|---|---|---|---|---|
Pathogenicity | 22977091 | Cord factors from atypical mycobacteria (Mycobacterium alvei, Mycobacterium brumae) stimulate the secretion of some pro-inflammatory cytokines of relevance in tuberculosis. | Linares C, Bernabeu A, Luquin M, Valero-Guillen PL | Microbiology (Reading) | 10.1099/mic.0.060681-0 | 2012 | Animals, Cell Line, Cord Factors/*metabolism/pharmacology, Cytokines/*metabolism, Humans, Inflammation Mediators/*metabolism, Mice, Nontuberculous Mycobacteria/*metabolism, Tuberculosis/immunology/*metabolism/microbiology, Up-Regulation/drug effects | Metabolism |
Genetics | 27125480 | Draft Genome Sequence of Mycobacterium brumae ATCC 51384. | D'Auria G, Torrents E, Luquin M, Comas I, Julian E | Genome Announc | 10.1128/genomeA.00237-16 | 2016 |
Reference
@id | authors | catalogue | doi/url | title | journal | pubmed |
---|---|---|---|---|---|---|
11575 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 44177) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-44177 | |||
20012 | Wink, J. | http://www.dsmz.de/microorganisms/wink_pdf/DSM44177.pdf | Compendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig | |||
20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |||
20218 | Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P. | 10.1016/j.syapm.2013.11.002 | StrainInfo introduces electronic passports for microorganisms. | Syst Appl Microbiol. 37: 42-50 2014 | 24321274 | |
40657 | Curators of the CIP | https://brclims.pasteur.fr/brcWeb/souche/detail/1/15265 | ||||
53751 | Curators of the CCUG | Culture Collection University of Gothenburg (CCUG) (CCUG 37586) | https://www.ccug.se/strain?id=37586 | |||
66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |||
66794 | Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg | 10.1093/nar/gkaa1025 | BRENDA, the ELIXIR core data resource in 2021: new developments and updates | Nucleic Acids Res. 49: D498-D508 2020 | 33211880 | |
67770 | Curators of the JCM | https://jcm.brc.riken.jp/en/ | ||||
68379 | Automatically annotated from API Coryne | |||||
68382 | Automatically annotated from API zym | |||||
69480 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |||
69481 | Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch | 10.21203/rs.3.rs-2527258/v1 | Predictions based on the model GenomeNet Sporulation v. 1 | |||
77844 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID46328.1 | StrainInfo: A central database for resolving microbial strain identifiers | |||
117061 | Curators of the CIP | Collection of Institut Pasteur (CIP 103465) | https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20103465 |