Strain identifier

BacDive ID: 8446

Type strain: Yes

Species: Mycobacterium brumae

Strain Designation: CR-270, 14 401 0001

Strain history: CIP <- 1993, V. Vincent, Inst. Pasteur, Paris, France: strain 14 401 0001 <- A. Ausina, Dept. Microbiol., Med. Univ., Barcelona, Spain: strain CR-270

For citation purpose refer to the digital object identifier (doi) of the current version.
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General

@ref: 11575

BacDive-ID: 8446

DSM-Number: 44177

keywords: genome sequence, 16S sequence, Bacteria, spore-forming, mesophilic

description: Mycobacterium brumae CR-270 is a spore-forming, mesophilic bacterium that was isolated from water sample.

NCBI tax id

NCBI tax idMatching level
85968species
1335619strain

strain history

@refhistory
11575<- CIP <- V. Vincent, Inst. Pasteur Paris <- A. Ausina, Dept. Microbiol., Med. Univ., Barcelona, CR-270
67770CECT 3022 <-- V. Ausina and M. Luquin CR-270.
117061CIP <- 1993, V. Vincent, Inst. Pasteur, Paris, France: strain 14 401 0001 <- A. Ausina, Dept. Microbiol., Med. Univ., Barcelona, Spain: strain CR-270

doi: 10.13145/bacdive8446.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Mycobacteriales
  • family: Mycobacteriaceae
  • genus: Mycobacterium
  • species: Mycobacterium brumae
  • full scientific name: Mycobacterium brumae Luquin et al. 1993
  • synonyms

    • @ref: 20215
    • synonym: Mycolicibacterium brumae

@ref: 11575

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Mycobacteriaceae

genus: Mycobacterium

species: Mycobacterium brumae

full scientific name: Mycobacterium brumae Luquin et al. 1993

strain designation: CR-270, 14 401 0001

type strain: yes

Morphology

cell morphology

@refmotilityconfidencegram staincell shape
69480no92.591
69480100positive
117061norod-shaped

colony morphology

@refcolony colorincubation periodmedium used
20012Cream (9001)10-14 days5006
20012Cream (9001)10-14 daysBHI
20012Cream (9001)10-14 daysBUG
20012Cream (9001)10-14 daysMB7H9

multicellular morphology

@refforms multicellular complexmedium name
20012noISP 2
20012noISP 3
20012noISP 4
20012noISP 5
20012noISP 6
20012noISP 7

multimedia

  • @ref: 11575
  • multimedia content: https://www.dsmz.de/microorganisms/photos/DSM_44177.jpg
  • caption: Medium 645 37°C
  • intellectual property rights: © Leibniz-Institut DSMZ

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
11575LÖWENSTEIN-JENSEN MEDIUM (DSMZ Medium 354)yeshttps://mediadive.dsmz.de/medium/354Name: LÖWENSTEIN-JENSEN MEDIUM (DSMZ Medium 354) Composition: Potato flour 18.6104 g/l L-Asparagin 2.23325 g/l KH2PO4 1.55087 g/l Mg-citrate 0.372208 g/l Malachite green 0.248139 g/l MgSO4 0.148883 g/l Glycerol Fresh egg mixture Distilled water
11575MIDDLEBROOK MEDIUM (DSMZ Medium 645)yeshttps://mediadive.dsmz.de/medium/645Name: MIDDLEBROOK MEDIUM (DSMZ Medium 645) Composition: Bacto Middlebrook 7H10 agar 20.9945 g/l Glycerol Distilled water
20012BUGyes
20012BHIyes
200125006yesMedium: 5006 Name: Composition (g/l) Sucrose 3,0 Dextrin 15,0 Meat extract 1,0 Yeast extract 2,0 Tryptone soy broth (Oxoid) 5,0 NaCl 0,5 K2HPO4 0,5 MgSO4 x 7 H2O 0,5 FeSO4 x 7 H2O 0,01 Agar 20,0 Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7,3 Usage: Maintenance Organisms: All Actinomycetes
20012MB7H9yesName: Middlebrook 7H9 (MB7H9) Broth Base Composition: Middelbrook 7H9 Broth Base 4.7 g/l Glycerol 2.0 g/l Casaminoacids 11.0 g/l Yeast extract 5.5 g/l Tween80 (20%) 2.5 g/l OADC-Enrichment 100 ml Agar 15.0 g/l Preparation: Dissolve in Milli-Q-H2O. Before Use add 10% OADC stock solution Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Maintenance and revitalization Organisms: Mycobacteria OADC stock solution old broth-name: OADC-Stock Composition: Bovine Serum Albumin, fraction V 5 % 1% Oleic Acid in 0.2N NaOH 5ml Glucose 2 % NaCl 0.85 % Comments: Melt 1% Oleic Acid in 65°C water bath. Dissolve all ingredients in 700ml of ddH2O. Adjust volume to 1L. Filter sterilize and store at 4°C. -To make 1% Oleic Acid in 0.2N NaOH: Dissolve 5g oleic acid (ampule) in 500ml 0.2N NaOH (300ml ddH2O + 20ml NaOH + adjust volume to 500ml). Heat solution to 55°C for oleic acid to melt. Store at -20°C in 50ml aliquots. There is no need to sterilize this. Sterilisation: 20 minutes Temperature:121°C PH before: PH after: Biological details Culture type: suitable for: Mycobacteria
40657MEDIUM 55 - for Mycobacteriumyes
117061CIP Medium 55yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=55

culture temp

@refgrowthtypetemperaturerange
20012positiveoptimum37mesophilic
11575positivegrowth37mesophilic
40657positivegrowth30mesophilic
67770positivegrowth30mesophilic
117061positivegrowth30-37mesophilic

Physiology and metabolism

spore formation

@refspore descriptionspore formationconfidence
20012Formation of sporangia (multiocular), spore surface smoothyes
69481no94
69480no99.975

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
11706116947citrate-carbon source
11706117632nitrate+reduction
6837917632nitrate-reduction
683794853esculin-hydrolysis
6837916199urea+hydrolysis
683795291gelatin-hydrolysis
6837917634D-glucose-fermentation
6837916988D-ribose-fermentation
6837965327D-xylose-fermentation
6837916899D-mannitol-fermentation
6837917306maltose-fermentation
6837917716lactose-fermentation
6837917992sucrose-fermentation
6837928087glycogen-fermentation

metabolite production

  • @ref: 117061
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

enzymes

@refvalueactivityec
68379gelatinase-
68379urease+3.5.1.5
68379beta-glucosidase-3.2.1.21
68379N-acetyl-beta-glucosaminidase-3.2.1.52
68379alpha-glucosidase-3.2.1.20
68379beta-galactosidase-3.2.1.23
68379beta-glucuronidase-3.2.1.31
68379alkaline phosphatase-3.1.3.1
68379pyrrolidonyl arylamidase-3.4.19.3
68379pyrazinamidase+3.5.1.B15
117061oxidase-
117061catalase+1.11.1.6
117061tween esterase+
117061urease+3.5.1.5
68382alkaline phosphatase-3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)+
68382lipase (C 14)-
68382leucine arylamidase+3.4.11.1
68382valine arylamidase-
68382cystine arylamidase-3.4.11.3
68382trypsin-3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase-3.1.3.2
68382naphthol-AS-BI-phosphohydrolase-
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase-3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase-3.2.1.20
68382beta-glucosidase-3.2.1.21
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API coryne

@refNITPYZPYRAPALbeta GURbeta GALalpha GLUbeta NAGESCUREGELGLURIBXYLMANMALLACSACGLYG
20012-+-------+---------

API zym

@refAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
20012-++-+--------------

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
117061++-----+--+--------+------------+--------------------------++-----------+-----+----+--+---+--------

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinent
11575water sampleBarcelonaSpainESPEurope
53751WaterBarcelonaSpainESPEurope
67770Water from the Llobregat RiverBarcelonaSpainESPEurope
117061Environment, Water sampleBarcelonaSpainESPEurope

isolation source categories

  • Cat1: #Environmental
  • Cat2: #Aquatic

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
115751Risk group (German classification)
200121German classification
1170611Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Mycobacterium brumae 16S ribosomal RNA gene, partial sequenceAF4805761449ena85968
20218Mycobacterium brumae strain CIP 103465 16S ribosomal RNA gene, partial sequenceAF547907539ena85968

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Mycolicibacterium brumae DSM 441771335619.3wgspatric1335619
66792Mycolicibacterium brumae DSM 441772866967103draftimg1335619
67770Mycolicibacterium brumae DSM 44177GCA_004014795contigncbi1335619
66792Mycolicibacterium brumae ATCC 51384GCA_025215495completencbi85968
66792Mycolicibacterium brumae DSM 44177GCA_025822525scaffoldncbi85968
66792Mycolicibacterium brumae strain ATCC 5138485968.9completepatric85968

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno94no
motileno95.898no
flagellatedno98.037no
gram-positiveyes88.492no
anaerobicno99.129no
aerobicyes81.608no
halophileno92.385no
spore-formingno88.426yes
glucose-fermentno89.477no
thermophileno97.711yes
glucose-utilyes86.33no

External links

@ref: 11575

culture collection no.: DSM 44177, ATCC 51384, CIP 103465, CCUG 37586, JCM 12273, CECT 3022, KCTC 19711

straininfo link

  • @ref: 77844
  • straininfo: 46328

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Pathogenicity22977091Cord factors from atypical mycobacteria (Mycobacterium alvei, Mycobacterium brumae) stimulate the secretion of some pro-inflammatory cytokines of relevance in tuberculosis.Linares C, Bernabeu A, Luquin M, Valero-Guillen PLMicrobiology (Reading)10.1099/mic.0.060681-02012Animals, Cell Line, Cord Factors/*metabolism/pharmacology, Cytokines/*metabolism, Humans, Inflammation Mediators/*metabolism, Mice, Nontuberculous Mycobacteria/*metabolism, Tuberculosis/immunology/*metabolism/microbiology, Up-Regulation/drug effectsMetabolism
Genetics27125480Draft Genome Sequence of Mycobacterium brumae ATCC 51384.D'Auria G, Torrents E, Luquin M, Comas I, Julian EGenome Announc10.1128/genomeA.00237-162016

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
11575Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 44177)https://www.dsmz.de/collection/catalogue/details/culture/DSM-44177
20012Wink, J.http://www.dsmz.de/microorganisms/wink_pdf/DSM44177.pdfCompendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
40657Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/15265
53751Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 37586)https://www.ccug.se/strain?id=37586
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
66794Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg10.1093/nar/gkaa1025BRENDA, the ELIXIR core data resource in 2021: new developments and updatesNucleic Acids Res. 49: D498-D508 202033211880
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68379Automatically annotated from API Coryne
68382Automatically annotated from API zym
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
77844Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID46328.1StrainInfo: A central database for resolving microbial strain identifiers
117061Curators of the CIPCollection of Institut Pasteur (CIP 103465)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20103465