Strain identifier

BacDive ID: 8441

Type strain: Yes

Species: Mycobacterium aichiense

Strain Designation: 49005

Strain history: CIP <- 2000, JCM <- M. Tsukamura: strain 49005

NCBI tax ID(s): 1799 (species)

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General

@ref: 11524

BacDive-ID: 8441

DSM-Number: 44147

keywords: genome sequence, 16S sequence, Bacteria, aerobe, mesophilic

description: Mycobacterium aichiense 49005 is an aerobe, mesophilic bacterium that was isolated from soil.

NCBI tax id

  • NCBI tax id: 1799
  • Matching level: species

strain history

@refhistory
11524<- P. Sander (Medizin. Hochschule Hannover) <- ATCC <- M. Tsukamura, 5545
67770M. Tsukamura 49005.
116165CIP <- 2000, JCM <- M. Tsukamura: strain 49005

doi: 10.13145/bacdive8441.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Mycobacteriales
  • family: Mycobacteriaceae
  • genus: Mycobacterium
  • species: Mycobacterium aichiense
  • full scientific name: Mycobacterium aichiense (ex Tsukamura 1973) Tsukamura 1981

  • synonyms

    • @ref: 20215
    • synonym: Mycolicibacterium aichiense

@ref: 11524

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Mycobacteriaceae

genus: Mycobacterium

species: Mycobacterium aichiense

full scientific name: Mycobacterium aichiense (ex Tsukamura 1973) Tsukamura 1981

strain designation: 49005

type strain: yes

Morphology

cell morphology

@refmotilityconfidencegram staincell shape
69480no92.077
69480100positive
116165nopositiverod-shaped

colony morphology

@refcolony colorincubation periodmedium used
19844Beige10-14 days5006
19844Beige10-14 daysBHI
19844Beige10-14 daysBUG
19844Beige10-14 daysMB7H9

multimedia

  • @ref: 11524
  • multimedia content: https://www.dsmz.de/microorganisms/photos/DSM_44147.jpg
  • caption: Medium 645 37°C
  • intellectual property rights: © Leibniz-Institut DSMZ

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
11524MIDDLEBROOK MEDIUM (DSMZ Medium 645)yeshttps://mediadive.dsmz.de/medium/645Name: MIDDLEBROOK MEDIUM (DSMZ Medium 645) Composition: Bacto Middlebrook 7H10 agar 20.9945 g/l Glycerol Distilled water
19844BUGyes
19844BHIyes
198445006yesMedium: 5006 Name: Composition (g/l) Sucrose 3,0 Dextrin 15,0 Meat extract 1,0 Yeast extract 2,0 Tryptone soy broth (Oxoid) 5,0 NaCl 0,5 K2HPO4 0,5 MgSO4 x 7 H2O 0,5 FeSO4 x 7 H2O 0,01 Agar 20,0 Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7,3 Usage: Maintenance Organisms: All Actinomycetes
19844MB7H9yesName: Middlebrook 7H9 (MB7H9) Broth Base Composition: Middelbrook 7H9 Broth Base 4.7 g/l Glycerol 2.0 g/l Casaminoacids 11.0 g/l Yeast extract 5.5 g/l Tween80 (20%) 2.5 g/l OADC-Enrichment 100 ml Agar 15.0 g/l Preparation: Dissolve in Milli-Q-H2O. Before Use add 10% OADC stock solution Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Maintenance and revitalization Organisms: Mycobacteria OADC stock solution old broth-name: OADC-Stock Composition: Bovine Serum Albumin, fraction V 5 % 1% Oleic Acid in 0.2N NaOH 5ml Glucose 2 % NaCl 0.85 % Comments: Melt 1% Oleic Acid in 65°C water bath. Dissolve all ingredients in 700ml of ddH2O. Adjust volume to 1L. Filter sterilize and store at 4°C. -To make 1% Oleic Acid in 0.2N NaOH: Dissolve 5g oleic acid (ampule) in 500ml 0.2N NaOH (300ml ddH2O + 20ml NaOH + adjust volume to 500ml). Heat solution to 55°C for oleic acid to melt. Store at -20°C in 50ml aliquots. There is no need to sterilize this. Sterilisation: 20 minutes Temperature:121°C PH before: PH after: Biological details Culture type: suitable for: Mycobacteria
36367MEDIUM 112 - for JanibacteryesDistilled water make up to (1000.000 ml);Magnesium sulphate heptahydrate (1.000 g);Agar (20.000 g);Yeast extract (5.000 g);Peptone (10.000 g);Tween 80 (50.000 mg);Glycerol (2.000 g);Malt extract (5.000 g);Beef extract (2.000 g);Casamino acids (5.000 g);Ca
116165CIP Medium 112yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=112

culture temp

@refgrowthtypetemperaturerange
19844positiveoptimum37mesophilic
11524positivegrowth37mesophilic
36367positivegrowth37mesophilic
53799positivegrowth30-37mesophilic
67770positivegrowth37mesophilic
116165positivegrowth22-37
116165nogrowth10psychrophilic
116165nogrowth45thermophilic

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
53799aerobe
53799microaerophile

spore formation

@refspore formationconfidence
69481no96
69480no99.98

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
116165606565hippurate-hydrolysis
11616517632nitrate-reduction
11616516301nitrite-reduction
11616517632nitrate-respiration

metabolite production

  • @ref: 116165
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

enzymes

@refvalueactivityec
116165oxidase-
116165beta-galactosidase+3.2.1.23
116165gelatinase-
116165catalase+1.11.1.6
116165gamma-glutamyltransferase-2.3.2.2
116165urease-3.5.1.5
68382alkaline phosphatase+3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)+
68382lipase (C 14)-
68382leucine arylamidase+3.4.11.1
68382valine arylamidase-
68382cystine arylamidase-3.4.11.3
68382trypsin-3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase-3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase+3.2.1.20
68382beta-glucosidase-3.2.1.21
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
116165-+++-+----++---+----

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinent
11524soil
53799Soil
67770SoilAichi Pref.JapanJPNAsia
116165Environment, Soil

isolation source categories

  • Cat1: #Environmental
  • Cat2: #Terrestrial
  • Cat3: #Soil

taxonmaps

  • @ref: 69479
  • File name: preview.99_2774.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_10;96_115;97_219;98_419;99_2774&stattab=map
  • Last taxonomy: Mycolicibacterium
  • 16S sequence: X55598
  • Sequence Identity:
  • Total samples: 372
  • soil counts: 136
  • aquatic counts: 31
  • animal counts: 53
  • plant counts: 152

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
115241Risk group (German classification)
198441Risk group (German classification)
1161651Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218M.aichiense 16S ribosomal RNA, partX555981456ena1799
20218Mycobacterium aichiense strain DSM 44147 16S ribosomal RNA gene, partial sequenceAF547894539ena1799

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Mycolicibacterium aichiense JCM 6376GCA_010726245completencbi1799
66792Mycolicibacterium aichiense strain JCM 63761799.7completepatric1799
66792Mycolicibacterium aichiense strain NCTC108201799.5wgspatric1799
66792Mycolicibacterium aichiense NCTC 108202856228341draftimg1799
67770Mycolicibacterium aichiense NCTC10820GCA_900453085contigncbi1799
66792Mycolicibacterium aichiense DSM 44147GCA_025822215contigncbi1799

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno96no
motileno92.678no
flagellatedno98.036no
gram-positiveyes88.223no
anaerobicno99.234yes
aerobicyes92.089no
halophileno93.778no
spore-formingno83.337no
glucose-utilyes82.798no
thermophileno99.561no
glucose-fermentno89.68no

External links

@ref: 11524

culture collection no.: DSM 44147, ATCC 27280, JCM 6376, NCTC 10820, CCUG 37672, LMG 19259, CIP 106808

straininfo link

  • @ref: 77840
  • straininfo: 13224

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
11524Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 44147)https://www.dsmz.de/collection/catalogue/details/culture/DSM-44147
19844Wink, J.http://www.dsmz.de/microorganisms/wink_pdf/DSM44147.pdfCompendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
36367Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/18980
53799Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 37672)https://www.ccug.se/strain?id=37672
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
77840Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID13224.1StrainInfo: A central database for resolving microbial strain identifiers
116165Curators of the CIPCollection of Institut Pasteur (CIP 106808)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20106808