Strain identifier

BacDive ID: 8436

Type strain: Yes

Species: Mycobacterium sphagni

Strain history: <- J. Kazda, Borstel; Sph 38

NCBI tax ID(s): 1786 (species)

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General

@ref: 11475

BacDive-ID: 8436

DSM-Number: 44076

keywords: genome sequence, 16S sequence, Bacteria, mesophilic

description: Mycobacterium sphagni DSM 44076 is a mesophilic bacterium that was isolated from sphagnum moss, Sphagnum recurvum.

NCBI tax id

  • NCBI tax id: 1786
  • Matching level: species

strain history

  • @ref: 11475
  • history: <- J. Kazda, Borstel; Sph 38

doi: 10.13145/bacdive8436.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Mycobacteriales
  • family: Mycobacteriaceae
  • genus: Mycobacterium
  • species: Mycobacterium sphagni
  • full scientific name: Mycobacterium sphagni Kazda 1980

  • synonyms

    • @ref: 20215
    • synonym: Mycolicibacterium sphagni

@ref: 11475

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Mycobacteriaceae

genus: Mycobacterium

species: Mycobacterium sphagni

full scientific name: Mycobacterium sphagni Kazda 1980

type strain: yes

Morphology

multimedia

  • @ref: 11475
  • multimedia content: https://www.dsmz.de/microorganisms/photos/DSM_44076.jpg
  • caption: Medium 645 28°C
  • intellectual property rights: © Leibniz-Institut DSMZ

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
11475LÖWENSTEIN-JENSEN MEDIUM (DSMZ Medium 354)yeshttps://mediadive.dsmz.de/medium/354Name: LÖWENSTEIN-JENSEN MEDIUM (DSMZ Medium 354) Composition: Potato flour 18.6104 g/l L-Asparagin 2.23325 g/l KH2PO4 1.55087 g/l Mg-citrate 0.372208 g/l Malachite green 0.248139 g/l MgSO4 0.148883 g/l Glycerol Fresh egg mixture Distilled water
11475MIDDLEBROOK MEDIUM (DSMZ Medium 645)yeshttps://mediadive.dsmz.de/medium/645Name: MIDDLEBROOK MEDIUM (DSMZ Medium 645) Composition: Bacto Middlebrook 7H10 agar 20.9945 g/l Glycerol Distilled water

culture temp

  • @ref: 11475
  • growth: positive
  • type: growth
  • temperature: 28
  • range: mesophilic

Physiology and metabolism

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6836925115malate-assimilation
6836917128adipate-assimilation
6836927689decanoate-assimilation
6836924265gluconate-assimilation
6836917306maltose-assimilation
6836959640N-acetylglucosamine-assimilation
6836916899D-mannitol-assimilation
6836916024D-mannose-assimilation
6836930849L-arabinose-assimilation
6836917634D-glucose-assimilation
683695291gelatin-hydrolysis
683694853esculin-hydrolysis
6836929016arginine-hydrolysis
6836917634D-glucose-fermentation
6836927897tryptophan-energy source
6836917632nitrate-reduction

metabolite production

  • @ref: 68369
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

metabolite tests

  • @ref: 68369
  • Chebi-ID: 35581
  • metabolite: indole
  • indole test: -

enzymes

@refvalueactivityec
68369gelatinase-
68369beta-glucosidase-3.2.1.21
68369arginine dihydrolase-3.5.3.6
68382alkaline phosphatase+3.1.3.1
68382esterase (C 4)-
68382esterase lipase (C 8)+
68382lipase (C 14)-
68382leucine arylamidase+3.4.11.1
68382valine arylamidase-
68382cystine arylamidase-3.4.11.3
68382trypsin-3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase-
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase-3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase-3.2.1.20
68382beta-glucosidase-3.2.1.21
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
11475-+-+-+----+---------

API 20NE

@refNO3TRPGLU_ FermADH ArgUREESCGELPNPGGLU_ AssimARAMNEMANNAGMALGNTCAPADIMLTCITPAC
11475----+/----------------

Isolation, sampling and environmental information

isolation

  • @ref: 11475
  • sample type: sphagnum moss, Sphagnum recurvum
  • host species: Sphagnum recurvum
  • geographic location: Salemer Moor
  • country: Germany
  • origin.country: DEU
  • continent: Europe

isolation source categories

  • Cat1: #Host
  • Cat2: #Plants
  • Cat3: #Peat moss

Safety information

risk assessment

  • @ref: 11475
  • biosafety level: 1
  • biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218M.sphagni 16S ribosomal RNA geneM59283146ena1786
20218Mycobacterium sphagni strain DSM 44076 16S ribosomal RNA gene, partial sequenceAF547968540ena1786
20218Mycobacterium sphagni partial 16S rRNA gene, type strain DSM44076TFR7337191505ena1786

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Mycolicibacterium sphagni ATCC 33027GCA_002250655contigncbi1786
66792Mycobacterium sphagni strain ATCC 330271786.3wgspatric1786
66792Mycolicibacterium sphagni ATCC 330272869221657draftimg1786
66792Mycolicibacterium sphagni DSM 44076GCA_025821515scaffoldncbi1786

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
motileno94.421no
gram-positiveyes90.375no
anaerobicno99.497no
aerobicyes91.324no
halophileno93.394no
spore-formingno87.544no
thermophileno99.537no
glucose-utilyes82.153no
flagellatedno98.392no
glucose-fermentno89.52no

External links

@ref: 11475

culture collection no.: DSM 44076, ATCC 33027, Sph 38

straininfo link

  • @ref: 77835
  • straininfo: 13220

literature

  • topic: Phylogeny
  • Pubmed-ID: 15388695
  • title: Mycobacterium psychrotolerans sp. nov., isolated from pond water near a uranium mine.
  • authors: Trujillo ME, Velazquez E, Kroppenstedt RM, Schumann P, Rivas R, Mateos PF, Martinez-Molina E
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijs.0.02938-0
  • year: 2004
  • mesh: Anti-Bacterial Agents/pharmacology, Carbohydrate Metabolism, Citric Acid/metabolism, DNA, Bacterial/chemistry/isolation & purification, DNA, Ribosomal/chemistry/isolation & purification, Drug Resistance, Multiple, Bacterial, Enzymes/analysis, Fatty Acids/analysis, Genes, rRNA, Mining, Molecular Sequence Data, Mycobacterium/*classification/genetics/*isolation & purification/physiology, Mycolic Acids/analysis, Phylogeny, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Sodium Chloride/pharmacology, Temperature, Uranium, *Water Microbiology
  • topic2: Enzymology

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
11475Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 44076)https://www.dsmz.de/collection/catalogue/details/culture/DSM-44076
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
68369Automatically annotated from API 20NE
68382Automatically annotated from API zym
77835Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID13220.1StrainInfo: A central database for resolving microbial strain identifiers