Strain identifier

BacDive ID: 8435

Type strain: Yes

Species: Mycobacterium neoaurum

Strain Designation: 3503, 14 125 0001

Strain history: CIP <- 1998, V. Vincent, Inst. Pasteur, Paris, France: strain 14 125 0001 <- ATCC <- M. Tsukamura: strain 3503

NCBI tax ID(s): 1265312 (strain), 1795 (species)

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General

@ref: 11473

BacDive-ID: 8435

DSM-Number: 44074

keywords: genome sequence, 16S sequence, Bacteria, aerobe, mesophilic, Gram-positive

description: Mycobacterium neoaurum 3503 is an aerobe, mesophilic, Gram-positive bacterium that was isolated from soil.

NCBI tax id

NCBI tax idMatching level
1265312strain
1795species

strain history

@refhistory
11473<- S. Rüsch-Gerdes, Res. Centre Borstel; 3503 <- ATCC; ATCC 25795 <- M. Tsukamura; 3503
67771<- CCUG <- JCM, RIKEN, Saitama, Japan 02 Apr 1997 <- 1987, M Tsukamura 10002 (=3503)
67770M. Tsukamura 10002 (=3503).
121265CIP <- 1998, V. Vincent, Inst. Pasteur, Paris, France: strain 14 125 0001 <- ATCC <- M. Tsukamura: strain 3503

doi: 10.13145/bacdive8435.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Mycobacteriales
  • family: Mycobacteriaceae
  • genus: Mycobacterium
  • species: Mycobacterium neoaurum
  • full scientific name: Mycobacterium neoaurum Tsukamura 1972 (Approved Lists 1980)

  • synonyms

    • @ref: 20215
    • synonym: Mycolicibacterium neoaurum

@ref: 11473

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Mycobacteriaceae

genus: Mycobacterium

species: Mycobacterium neoaurum

full scientific name: Mycobacterium neoaurum Tsukamura 1972

strain designation: 3503, 14 125 0001

type strain: yes

Morphology

cell morphology

@refgram staincell shapemotility
67771positive
121265rod-shapedno

colony morphology

@refcolony colorincubation periodmedium used
19995Daffodil yellow (1007)10-14 days5006
19995Daffodil yellow (1007)10-14 daysMB7H10
19995Daffodil yellow (1007)10-14 daysMB7H11

multimedia

  • @ref: 11473
  • multimedia content: https://www.dsmz.de/microorganisms/photos/DSM_44074.jpg
  • caption: Medium 645 37°C
  • intellectual property rights: © Leibniz-Institut DSMZ

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
11473MIDDLEBROOK MEDIUM (DSMZ Medium 645)yeshttps://mediadive.dsmz.de/medium/645Name: MIDDLEBROOK MEDIUM (DSMZ Medium 645) Composition: Bacto Middlebrook 7H10 agar 20.9945 g/l Glycerol Distilled water
19995MB7H10yesName: Middlebrook 7H10 (MB7H10) Broth Base Composition: amount in percent Middlebrook 7H10 Agar 1.9% Glycerol 0.5% OADC-Enrichment in ml (NL5633) 10ml Comments: Add broth base and glycerol to ddh2O. Adjust volumes. Autoclave. Cool to 42°C. Add OADC-stock. Sterilisation: 20 minutes Temperature:121°C PH before: PH after: Biological details Culture type: suitable for: Mycobacteria OADC stock solution old broth-name: OADC-Stock Composition: Bovine Serum Albumin, fraction V 5 % 1% Oleic Acid in 0.2N NaOH 5ml Glucose 2 % NaCl 0.85 % Comments: Melt 1% Oleic Acid in 65°C water bath. Dissolve all ingredients in 700ml of ddH2O. Adjust volume to 1L. Filter sterilize and store at 4°C. -To make 1% Oleic Acid in 0.2N NaOH: Dissolve 5g oleic acid (ampule) in 500ml 0.2N NaOH (300ml ddH2O + 20ml NaOH + adjust volume to 500ml). Heat solution to 55°C for oleic acid to melt. Store at -20°C in 50ml aliquots. There is no need to sterilize this. Sterilisation: 20 minutes Temperature:121°C PH before: PH after: Biological details Culture type: suitable for: Mycobacteria
19995MB7H11yesName: Middlebrook 7H11 (MB7H11) Broth Base Composition: Middlebrook 7H11 Agar 2% Glycerol 0.5% OADC-Enrichment 10ml Comments: Add broth base and glycerol to ddH2O. Adjust volumes. Autoclave. Cool to 42°C. Add OADC-Stock. Sterilisation: 20 minutes Temperature:121°C PH before: PH after: Biological details Culture type: suitable for: Mycobacteria OADC stock solution old broth-name: OADC-Stock Composition: Bovine Serum Albumin, fraction V 5 % 1% Oleic Acid in 0.2N NaOH 5ml Glucose 2 % NaCl 0.85 % Comments: Melt 1% Oleic Acid in 65°C water bath. Dissolve all ingredients in 700ml of ddH2O. Adjust volume to 1L. Filter sterilize and store at 4°C. -To make 1% Oleic Acid in 0.2N NaOH: Dissolve 5g oleic acid (ampule) in 500ml 0.2N NaOH (300ml ddH2O + 20ml NaOH + adjust volume to 500ml). Heat solution to 55°C for oleic acid to melt. Store at -20°C in 50ml aliquots. There is no need to sterilize this. Sterilisation: 20 minutes Temperature:121°C PH before: PH after: Biological details Culture type: suitable for: Mycobacteria
199955006yesMedium: 5006 Name: Composition (g/l) Sucrose 3,0 Dextrin 15,0 Meat extract 1,0 Yeast extract 2,0 Tryptone soy broth (Oxoid) 5,0 NaCl 0,5 K2HPO4 0,5 MgSO4 x 7 H2O 0,5 FeSO4 x 7 H2O 0,01 Agar 20,0 Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7,3 Usage: Maintenance Organisms: All Actinomycetes
36383MEDIUM 55 - for Mycobacteriumyes
121265CIP Medium 55yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=55

culture temp

@refgrowthtypetemperaturerange
11473positivegrowth37mesophilic
19995positiveoptimum37mesophilic
36383positivegrowth37mesophilic
53793positivegrowth30-37mesophilic
67770positivegrowth37mesophilic
67771positivegrowth37mesophilic
121265positivegrowth22-37
121265nogrowth10psychrophilic
121265nogrowth45thermophilic

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
53793aerobe
67771aerobe
121265obligate aerobe

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
121265606565hippurate-hydrolysis
12126517632nitrate+reduction
12126516301nitrite-reduction
12126517632nitrate-respiration
6837916199urea+hydrolysis
683795291gelatin-hydrolysis
6837917634D-glucose-fermentation
6837916988D-ribose-fermentation
6837965327D-xylose-fermentation
6837916899D-mannitol-fermentation
6837917306maltose-fermentation
6837917716lactose-fermentation
6837917992sucrose-fermentation
6837928087glycogen-fermentation

metabolite production

  • @ref: 121265
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

enzymes

@refvalueactivityec
68379catalase+1.11.1.6
68379gelatinase-
68379urease+3.5.1.5
68379N-acetyl-beta-glucosaminidase-3.2.1.52
68379beta-galactosidase-3.2.1.23
68379beta-glucuronidase-3.2.1.31
68379pyrrolidonyl arylamidase+3.4.19.3
68379pyrazinamidase+3.5.1.B15
121265oxidase-
121265beta-galactosidase-3.2.1.23
121265gelatinase-
121265amylase-
121265DNase-
121265caseinase-3.4.21.50
121265catalase+1.11.1.6
121265tween esterase+
121265gamma-glutamyltransferase-2.3.2.2
121265lecithinase-
121265lipase-
121265protease-
121265urease+3.5.1.5
68382alkaline phosphatase-3.1.3.1
68382esterase lipase (C 8)+
68382lipase (C 14)-
68382leucine arylamidase+3.4.11.1
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase-3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-fucosidase-3.2.1.51

API coryne

@refNITPYZPYRAPALbeta GURbeta GALalpha GLUbeta NAGESCUREGELGLURIBXYLMANMALLACSACGLYGControlCAT
19995-++------+---------
53793++++--+-++----------+

API zym

@refAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- FucosidaseControl
19995-++-+--+-++---+--+-
53793--+-++++-++----+----
121265-++-+-+--++---------

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
121265+++++---------------++---------+++--------------++++--------+-----------+--+--+++---+-+-+------++-+

Isolation, sampling and environmental information

isolation

@refsample type
11473soil
53793Soil
67770Soil
67771From soil
121265Environment, Soil

isolation source categories

  • Cat1: #Environmental
  • Cat2: #Terrestrial
  • Cat3: #Soil

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
114732Risk group (German classification)
199951German classification
1212651Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Mycobacterium neoaurum 16S ribosomal RNA gene, partial sequenceAF4805931470ena1265312
20218Mycobacterium neoaurum strain ATCC 25795 16S ribosomal RNA gene, partial sequenceFJ1723061451ena1265312
20218M.neoaurum 16S ribosomal RNAM295641354ena1795
20218Mycobacterium neoaurum BCRC:17230 16S ribosomal RNA gene, partial sequence; 16S-23S ribosomal RNA intergenic spacer, complete sequence; and 23S ribosomal RNA gene, partial sequenceHM584726272ena1795
20218Mycobacterium neoaurum strain CIP 105387 16S ribosomal RNA gene, partial sequenceAF547951540ena1265312

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Mycobacterium neoaurum ATCC 257951265312.4wgspatric1265312
66792Mycobacterium neoaurum strain ATCC 257951795.5wgspatric1795
66792Mycolicibacterium neoaurum strain DSM 440741795.15wgspatric1795
66792Mycolicibacterium neoaurum ATCC 257952582581012draftimg1265312
66792Mycolicibacterium neoaurum ATCC 257952600255158draftimg1265312
67770Mycolicibacterium neoaurum DSM 44074GCA_000724065scaffoldncbi1795
67770Mycolicibacterium neoaurum ATCC 25795GCA_000691525contigncbi1265312
67770Mycolicibacterium neoaurum DSM 44074GCA_005670605scaffoldncbi1795

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
motileno94.195no
gram-positiveyes90.593no
anaerobicno99.575yes
aerobicyes91.006no
halophileno91.933no
spore-formingno82.008no
glucose-utilyes88.162no
flagellatedno98.318no
thermophileno99.581no
glucose-fermentno88.731yes

External links

@ref: 11473

culture collection no.: DSM 44074, ATCC 25795, JCM 6365, NCTC 10818, CCUG 37665, CIP 105387, LMG 19258, KCTC 19096, BCRC 17230, HAMBI 2273

straininfo link

  • @ref: 77834
  • straininfo: 14097

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Enzymology16349147Purification and Characterization of an l-Amino Amidase from Mycobacterium neoaurum ATCC 25795.Hermes HF, Tandler RF, Sonke T, Dijkhuizen L, Meijer EMAppl Environ Microbiol10.1128/aem.60.1.153-159.19941994
Phylogeny23728378Mycobacterium sediminis sp. nov. and Mycobacterium arabiense sp. nov., two rapidly growing members of the genus Mycobacterium.Zhang DF, Chen X, Zhang XM, Zhi XY, Yao JC, Jiang Y, Xiong Z, Li WJInt J Syst Evol Microbiol10.1099/ijs.0.050567-02013Bacterial Typing Techniques, Base Composition, China, DNA, Bacterial/genetics, DNA, Ribosomal Spacer/genetics, Fatty Acids/chemistry, Genes, Bacterial, Geologic Sediments/*microbiology, Molecular Sequence Data, Multilocus Sequence Typing, Mycobacterium/*classification/genetics/isolation & purification, Nucleic Acid Hybridization, Oceans and Seas, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, United Arab EmiratesGenetics
Metabolism24831710Characterization and engineering of 3-ketosteroid- big up tri, open1-dehydrogenase and 3-ketosteroid-9alpha-hydroxylase in Mycobacterium neoaurum ATCC 25795 to produce 9alpha-hydroxy-4-androstene-3,17-dione through the catabolism of sterols.Yao K, Xu LQ, Wang FQ, Wei DZMetab Eng10.1016/j.ymben.2014.05.0052014Androstenedione/*analogs & derivatives/biosynthesis, *Bacterial Proteins/genetics/metabolism, *Mixed Function Oxygenases/genetics/metabolism, *Mycobacterium/enzymology/genetics, *Oxidoreductases/genetics/metabolism, Phytosterols/metabolismEnzymology
Genetics25013147Draft Genome Sequence of Mycobacterium neoaurum Strain DSM 44074T.Phelippeau M, Robert C, Croce O, Raoult D, Drancourt MGenome Announc10.1128/genomeA.00699-142014
Metabolism26898409Unraveling and engineering the production of 23,24-bisnorcholenic steroids in sterol metabolism.Xu LQ, Liu YJ, Yao K, Liu HH, Tao XY, Wang FQ, Wei DZSci Rep10.1038/srep21928201617-Hydroxysteroid Dehydrogenases/genetics/metabolism, 3-Hydroxyacyl CoA Dehydrogenases/genetics/metabolism, Biotransformation, Cholenes/metabolism, Genes, Bacterial, Industrial Microbiology, Metabolic Engineering, Mycobacterium/genetics/*metabolism, Steroids/biosynthesis, Sterols/*metabolismBiotechnology
Metabolism28532497Improving the production of 22-hydroxy-23,24-bisnorchol-4-ene-3-one from sterols in Mycobacterium neoaurum by increasing cell permeability and modifying multiple genes.Xiong LB, Liu HH, Xu LQ, Sun WJ, Wang FQ, Wei DZMicrob Cell Fact10.1186/s12934-017-0705-x2017Cell Membrane Permeability, Cholestenones/*metabolism, *Genes, Bacterial, Metabolic Networks and Pathways, Mycobacterium/*genetics/*metabolism, Sterols/*chemistry/*metabolismBiotechnology
Metabolism29728384Engineered 3-Ketosteroid 9alpha-Hydroxylases in Mycobacterium neoaurum: an Efficient Platform for Production of Steroid Drugs.Liu HH, Xu LQ, Yao K, Xiong LB, Tao XY, Liu M, Wang FQ, Wei DZAppl Environ Microbiol10.1128/AEM.02777-172018Amino Acid Substitution, Androstadienes/metabolism, Bacterial Proteins/*genetics/*metabolism, Biotransformation, Cholesterol, Diosgenin/metabolism, Gene Deletion, Genetic Engineering/methods, Metabolic Networks and Pathways/genetics, Mixed Function Oxygenases/*genetics, Models, Molecular, Mutagenesis, Site-Directed, Mycobacterium/*genetics/*metabolism, Nontuberculous Mycobacteria/genetics/metabolism, Oxygenases/metabolism, Phytosterols/metabolism, Sequence Alignment, Sequence Analysis, Protein, Steroids/*metabolismBiotechnology
Metabolism30075077Integrated Transcriptome and Proteome Studies Reveal the Underlying Mechanisms for Sterol Catabolism and Steroid Production in Mycobacterium neoaurum.Liu M, Xiong LB, Tao X, Liu QH, Wang FQ, Wei DZJ Agric Food Chem10.1021/acs.jafc.8b027142018Bacterial Proteins/*genetics/metabolism, Gene Expression Regulation, Bacterial, Multigene Family, Mycobacterium/chemistry/genetics/*metabolism, Proteome/*genetics/metabolism, Regulon, Steroids/*biosynthesis/chemistry, Sterols/chemistry/*metabolism, TranscriptomeTranscriptome
Metabolism30362748Metabolic Adaptation of Mycobacterium neoaurum ATCC 25795 in the Catabolism of Sterols for Producing Important Steroid Intermediates.Liu M, Xiong LB, Tao X, Liu QH, Wang FQ, Wei DZJ Agric Food Chem10.1021/acs.jafc.8b047772018Acyl Coenzyme A/metabolism, Bacterial Proteins/genetics/metabolism, Mycobacterium/chemistry/genetics/*metabolism, Steroids/chemistry/*metabolism, Sterols/chemistry/*metabolism
Metabolism32228591Enhancing the bioconversion of phytosterols to steroidal intermediates by the deficiency of kasB in the cell wall synthesis of Mycobacterium neoaurum.Xiong LB, Liu HH, Zhao M, Liu YJ, Song L, Xie ZY, Xu YX, Wang FQ, Wei DZMicrob Cell Fact10.1186/s12934-020-01335-y2020Cell Wall/*metabolism, Mycobacteriaceae/*chemistry, Phytosterols/*metabolism, Steroids/*metabolism
34653700CRISPR-Cas12a assisted precise genome editing of Mycolicibacterium neoaurum.Liu K, Gao Y, Li ZH, Liu M, Wang FQ, Wei DZN Biotechnol10.1016/j.nbt.2021.10.0032021*CRISPR-Cas Systems/genetics, DNA End-Joining Repair, *Gene Editing, Mycobacteriaceae/*genetics, Plasmids
Metabolism34949197Production of 9,21-dihydroxy-20-methyl-pregna-4-en-3-one from phytosterols in Mycobacterium neoaurum by modifying multiple genes and improving the intracellular environment.Yuan CY, Ma ZG, Zhang JX, Liu XC, Du GL, Sun JS, Shi JP, Zhang BGMicrob Cell Fact10.1186/s12934-021-01717-w2021Bacterial Proteins/genetics, Coenzyme A-Transferases/genetics, Gene Editing, Gene Knockout Techniques, Genome, Bacterial, Hydro-Lyases/genetics, *Metabolic Networks and Pathways, Mycobacteriaceae/*genetics/*metabolism, Oxidoreductases/genetics, Phytosterols/*metabolism, Prodrugs/*metabolism, Steroids/*metabolismGenetics
Biotechnology35782483Multiplexed site-specific genome engineering in Mycolicibacterium neoaurum by Att/Int system.Liu K, Lin GH, Liu K, Liu YJ, Tao XY, Gao B, Zhao M, Wei DZ, Wang FQSynth Syst Biotechnol10.1016/j.synbio.2022.05.0062022

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
11473Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 44074)https://www.dsmz.de/collection/catalogue/details/culture/DSM-44074
19995Wink, J.http://www.dsmz.de/microorganisms/wink_pdf/DSM44074.pdfCompendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
36383Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/17401
53793Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 37665)https://www.ccug.se/strain?id=37665
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
67771Curators of the KCTChttps://kctc.kribb.re.kr/En/Kctc
68379Automatically annotated from API Coryne
68382Automatically annotated from API zym
77834Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID14097.1StrainInfo: A central database for resolving microbial strain identifiers
121265Curators of the CIPCollection of Institut Pasteur (CIP 105387)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20105387