Strain identifier

BacDive ID: 8428

Type strain: Yes

Species: Mycobacterium malmoense

Strain Designation: Mo 816, Mo 816 (TMC 802)

Strain history: CIP <- 1998, ATCC <- I. Juhlin: strain Mo 816 (TMC 802)

NCBI tax ID(s): 1780 (species)

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General

@ref: 11561

BacDive-ID: 8428

DSM-Number: 44163

keywords: genome sequence, 16S sequence, Bacteria, microaerophile, mesophilic, Gram-positive, human pathogen

description: Mycobacterium malmoense Mo 816 is a microaerophile, mesophilic, Gram-positive human pathogen that was isolated from lung tissue.

NCBI tax id

  • NCBI tax id: 1780
  • Matching level: species

strain history

@refhistory
11561<- ATCC; ATCC 29571 <- I. Juhlin; Mo 816
67770CCUG 37761 <-- I. Tjernberg <-- ATCC 29571 <-- I. Juhlin M-ö 816.
120761CIP <- 1998, ATCC <- I. Juhlin: strain Mo 816 (TMC 802)

doi: 10.13145/bacdive8428.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Mycobacteriales
  • family: Mycobacteriaceae
  • genus: Mycobacterium
  • species: Mycobacterium malmoense
  • full scientific name: Mycobacterium malmoense Schröder and Juhlin 1977 (Approved Lists 1980)

@ref: 11561

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Mycobacteriaceae

genus: Mycobacterium

species: Mycobacterium malmoense

full scientific name: Mycobacterium malmoense Schröder and Juhlin 1977

strain designation: Mo 816, Mo 816 (TMC 802)

type strain: yes

Morphology

cell morphology

@refgram stainconfidencecell shapemotility
69480positive100
120761rod-shapedno

colony morphology

  • @ref: 53850
  • incubation period: 21-28 days

multimedia

@refmultimedia contentintellectual property rightscaption
11561https://www.dsmz.de/microorganisms/photos/DSM_44163-1.jpg© Leibniz-Institut DSMZ
11561https://www.dsmz.de/microorganisms/photos/DSM_44163.jpg© Leibniz-Institut DSMZMedium 645 37°C

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
11561MIDDLEBROOK MEDIUM (DSMZ Medium 645)yeshttps://mediadive.dsmz.de/medium/645Name: MIDDLEBROOK MEDIUM (DSMZ Medium 645) Composition: Bacto Middlebrook 7H10 agar 20.9945 g/l Glycerol Distilled water
36348MEDIUM 55 - for Mycobacteriumyes
120761CIP Medium 55yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=55
120761CIP Medium 267yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=267

culture temp

@refgrowthtypetemperaturerange
11561positivegrowth37mesophilic
36348positivegrowth37mesophilic
53850positivegrowth37mesophilic
67770positivegrowth37mesophilic
120761positivegrowth22-37
120761nogrowth10psychrophilic
120761nogrowth45thermophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 53850
  • oxygen tolerance: microaerophile

spore formation

@refspore formationconfidence
69481no95
69480no98.547

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
120761606565hippurate-hydrolysis
12076117632nitrate-reduction
12076116301nitrite-reduction

metabolite production

  • @ref: 120761
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

enzymes

@refvalueactivityec
120761oxidase-
120761beta-galactosidase-3.2.1.23
120761gelatinase-
120761catalase+1.11.1.6
120761gamma-glutamyltransferase-2.3.2.2
120761urease-3.5.1.5
68382alkaline phosphatase-3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)+
68382lipase (C 14)-
68382valine arylamidase-
68382cystine arylamidase-3.4.11.3
68382trypsin-3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase-3.1.3.2
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase-3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase-3.2.1.20
68382beta-glucosidase-3.2.1.21
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

fatty acid profile

  • fatty acids

    @reffatty acidpercentageECL
    53850C14:02.814
    53850C15:01.315
    53850C16:032.616
    53850C17:01.117
    53850C18:07.518
    53850C20:01.520
    53850C16:1 ω7c/C15:0 ISO 2OH11.615.85
    53850C16:1 ω9c1.815.774
    53850C17:1 ω8c0.316.792
    53850C17:1 ω9c ISO0.616.416
    53850C18:1 ω9c19.217.769
    53850C19:0 CYCLO ω9c2.118.87
    53850TBSA 10Me18:017.718.392
  • type of FA analysis: whole cell analysis
  • method/protocol: CCUG

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
11561--++-+-----+/---------
120761--++-------+--------

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinent
11561lung tissue
53850Human lung tissueMalmöSwedenSWEEurope
67770Resected lung tissueMalmöSwedenSWEEurope
120761Lung tissueSwedenSWEEurope

isolation source categories

Cat1Cat2Cat3
#Host#Mammals
#Host Body-Site#Oral cavity and airways#Lung

taxonmaps

  • @ref: 69479
  • File name: preview.99_75.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_10;96_37;97_39;98_43;99_75&stattab=map
  • Last taxonomy: Mycobacterium
  • 16S sequence: X52930
  • Sequence Identity:
  • Total samples: 1520
  • soil counts: 881
  • aquatic counts: 382
  • animal counts: 203
  • plant counts: 54

Safety information

risk assessment

@refpathogenicity humanpathogenicity animalbiosafety levelbiosafety level comment
11561yesyes2Risk group (German classification)
1207612Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Mycobacterium malmoense strain ATCC 29571 16S ribosomal RNA gene, partial sequenceGQ1532781495ena1780
20218M.malmoense 16S ribosomal RNA geneM59275145ena1780
20218M.malmoense 16S ribosomal rRNAM616661367ena1780
20218Mycobacterium malmoense 16S rRNA geneX529301463ena1780
20218Mycobacterium malmoense strain CIP 105775 16S ribosomal RNA gene, partial sequenceAF547945554ena1780
20218Mycobacterium malmoense DNA for 16S rRNA, internal transcribed spacer, 23S rRNAAB026696432ena1780

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Mycobacterium malmoense ATCC 29571GCA_019645855completencbi1780
66792Mycobacterium malmoense strain ATCC 295711780.25completepatric1780
66792Mycobacterium malmoense DSM 44163GCA_022558505completencbi1780

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno95no
flagellatedno98.956no
gram-positiveyes89.057no
anaerobicno99.003no
aerobicyes87.052no
halophileno91.28no
spore-formingno83.348no
motileno95.298no
thermophileno95.676yes
glucose-utilyes65.709no
glucose-fermentno90.651no

External links

@ref: 11561

culture collection no.: DSM 44163, ATCC 29571, TMC 802, CCUG 37761, JCM 13391, CIP 105775, NCTC 11298

straininfo link

  • @ref: 77827
  • straininfo: 39593

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Pathogenicity3659578Mycobacterium malmoense--report of a case in Switzerland.Leuenberger P, Aubert JD, Beer VRespiration10.1159/0001952151987Bronchoalveolar Lavage Fluid/microbiology, Erythromycin/therapeutic use, Humans, Isoniazid/therapeutic use, Male, Microbial Sensitivity Tests, Middle Aged, Mycobacterium/*isolation & purification, Mycobacterium Infections/*etiology, Mycobacterium Infections, Nontuberculous/*etiology/therapy, Nontuberculous Mycobacteria/drug effects/*isolation & purification, Pneumonectomy, Rifampin/therapeutic use, Sputum/microbiology, SwitzerlandEnzymology
Phylogeny20833878Mycobacterium shinjukuense sp. nov., a slowly growing, non-chromogenic species isolated from human clinical specimens.Saito H, Iwamoto T, Ohkusu K, Otsuka Y, Akiyama Y, Sato S, Taguchi O, Sueyasu Y, Kawabe Y, Fujimoto H, Ezaki T, Butler RInt J Syst Evol Microbiol10.1099/ijs.0.025478-02010Aged, Aged, 80 and over, Bacterial Proteins/genetics, DNA, Bacterial/genetics, DNA, Ribosomal/genetics, Female, Humans, Male, Middle Aged, Molecular Sequence Data, Mycobacterium/*classification/genetics/growth & development/*isolation & purification, Mycobacterium Infections/*microbiology, Phylogeny, RNA, Ribosomal, 16S/geneticsPathogenicity

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
11561Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 44163)https://www.dsmz.de/collection/catalogue/details/culture/DSM-44163
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
36348Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/17831
53850Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 37761)https://www.ccug.se/strain?id=37761
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
77827Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID39593.1StrainInfo: A central database for resolving microbial strain identifiers
120761Curators of the CIPCollection of Institut Pasteur (CIP 105775)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20105775