Strain identifier

BacDive ID: 8425

Type strain: Yes

Species: Mycobacterium thermoresistibile

Strain Designation: 14 131 0001, 316

Strain history: CIP <- 1998, V. Vincent, Inst. Pasteur, Paris, France: strain 14 131 0001 <- ATCC <- M. Tsukamura: strain 316

NCBI tax ID(s): 1078020 (strain), 1797 (species)

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General

@ref: 11565

BacDive-ID: 8425

DSM-Number: 44167

keywords: genome sequence, 16S sequence, Bacteria, obligate aerobe, mesophilic, human pathogen

description: Mycobacterium thermoresistibile 14 131 0001 is an obligate aerobe, mesophilic human pathogen that was isolated from soil.

NCBI tax id

NCBI tax idMatching level
1797species
1078020strain

strain history

@refhistory
11565<- ATCC <- M. Tsukamura, 316 (01039)
67770M. Tsukamura 01039 (=316).
123512CIP <- 1998, V. Vincent, Inst. Pasteur, Paris, France: strain 14 131 0001 <- ATCC <- M. Tsukamura: strain 316

doi: 10.13145/bacdive8425.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Mycobacteriales
  • family: Mycobacteriaceae
  • genus: Mycobacterium
  • species: Mycobacterium thermoresistibile
  • full scientific name: Mycobacterium thermoresistibile Tsukamura 1966 (Approved Lists 1980)

  • synonyms

    • @ref: 20215
    • synonym: Mycolicibacterium thermoresistibile

@ref: 11565

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Mycobacteriaceae

genus: Mycobacterium

species: Mycobacterium thermoresistibile

full scientific name: Mycobacterium thermoresistibile Tsukamura 1966 emend. Nouioui et al. 2018

strain designation: 14 131 0001, 316

type strain: yes

Morphology

cell morphology

@refmotilityconfidencegram staincell shape
69480no92.037
69480100positive
123512norod-shaped

colony morphology

@refcolony colorincubation periodmedium used
20011Signal yellow (1003)10-14 days5006
20011Signal yellow (1003)10-14 daysMB7H10
20011Signal yellow (1003)10-14 daysMB7H11

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
11565LÖWENSTEIN-JENSEN MEDIUM (DSMZ Medium 354)yeshttps://mediadive.dsmz.de/medium/354Name: LÖWENSTEIN-JENSEN MEDIUM (DSMZ Medium 354) Composition: Potato flour 18.6104 g/l L-Asparagin 2.23325 g/l KH2PO4 1.55087 g/l Mg-citrate 0.372208 g/l Malachite green 0.248139 g/l MgSO4 0.148883 g/l Glycerol Fresh egg mixture Distilled water
11565MIDDLEBROOK MEDIUM (DSMZ Medium 645)yeshttps://mediadive.dsmz.de/medium/645Name: MIDDLEBROOK MEDIUM (DSMZ Medium 645) Composition: Bacto Middlebrook 7H10 agar 20.9945 g/l Glycerol Distilled water
20011MB7H10yesName: Middlebrook 7H10 (MB7H10) Broth Base Composition: amount in percent Middlebrook 7H10 Agar 1.9% Glycerol 0.5% OADC-Enrichment in ml (NL5633) 10ml Comments: Add broth base and glycerol to ddh2O. Adjust volumes. Autoclave. Cool to 42°C. Add OADC-stock. Sterilisation: 20 minutes Temperature:121°C PH before: PH after: Biological details Culture type: suitable for: Mycobacteria OADC stock solution old broth-name: OADC-Stock Composition: Bovine Serum Albumin, fraction V 5 % 1% Oleic Acid in 0.2N NaOH 5ml Glucose 2 % NaCl 0.85 % Comments: Melt 1% Oleic Acid in 65°C water bath. Dissolve all ingredients in 700ml of ddH2O. Adjust volume to 1L. Filter sterilize and store at 4°C. -To make 1% Oleic Acid in 0.2N NaOH: Dissolve 5g oleic acid (ampule) in 500ml 0.2N NaOH (300ml ddH2O + 20ml NaOH + adjust volume to 500ml). Heat solution to 55°C for oleic acid to melt. Store at -20°C in 50ml aliquots. There is no need to sterilize this. Sterilisation: 20 minutes Temperature:121°C PH before: PH after: Biological details Culture type: suitable for: Mycobacteria
20011MB7H11yesName: Middlebrook 7H11 (MB7H11) Broth Base Composition: Middlebrook 7H11 Agar 2% Glycerol 0.5% OADC-Enrichment 10ml Comments: Add broth base and glycerol to ddH2O. Adjust volumes. Autoclave. Cool to 42°C. Add OADC-Stock. Sterilisation: 20 minutes Temperature:121°C PH before: PH after: Biological details Culture type: suitable for: Mycobacteria OADC stock solution old broth-name: OADC-Stock Composition: Bovine Serum Albumin, fraction V 5 % 1% Oleic Acid in 0.2N NaOH 5ml Glucose 2 % NaCl 0.85 % Comments: Melt 1% Oleic Acid in 65°C water bath. Dissolve all ingredients in 700ml of ddH2O. Adjust volume to 1L. Filter sterilize and store at 4°C. -To make 1% Oleic Acid in 0.2N NaOH: Dissolve 5g oleic acid (ampule) in 500ml 0.2N NaOH (300ml ddH2O + 20ml NaOH + adjust volume to 500ml). Heat solution to 55°C for oleic acid to melt. Store at -20°C in 50ml aliquots. There is no need to sterilize this. Sterilisation: 20 minutes Temperature:121°C PH before: PH after: Biological details Culture type: suitable for: Mycobacteria
200115006yesMedium: 5006 Name: Composition (g/l) Sucrose 3,0 Dextrin 15,0 Meat extract 1,0 Yeast extract 2,0 Tryptone soy broth (Oxoid) 5,0 NaCl 0,5 K2HPO4 0,5 MgSO4 x 7 H2O 0,5 FeSO4 x 7 H2O 0,01 Agar 20,0 Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7,3 Usage: Maintenance Organisms: All Actinomycetes
36375MEDIUM 55 - for Mycobacteriumyes
123512CIP Medium 55yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=55

culture temp

@refgrowthtypetemperaturerange
20011positiveoptimum37mesophilic
11565positivegrowth37mesophilic
36375positivegrowth37mesophilic
67770positivegrowth37mesophilic
123512positivegrowth25-45
123512nogrowth10psychrophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 123512
  • oxygen tolerance: obligate aerobe

spore formation

  • @ref: 69480
  • spore formation: no
  • confidence: 99.525

observation

  • @ref: 67770
  • observation: quinones: MK-9(H2)

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
123512606565hippurate-hydrolysis
12351217632nitrate+reduction
12351216301nitrite-reduction
12351217632nitrate-respiration
6837917632nitrate-reduction
683794853esculin-hydrolysis
6837916199urea-hydrolysis
683795291gelatin-hydrolysis
6837917634D-glucose-fermentation
6837916988D-ribose-fermentation
6837965327D-xylose-fermentation
6837916899D-mannitol-fermentation
6837917306maltose-fermentation
6837917716lactose-fermentation
6837917992sucrose+fermentation
6837928087glycogen-fermentation

metabolite production

  • @ref: 123512
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

enzymes

@refvalueactivityec
68379gelatinase-
68379urease-3.5.1.5
68379beta-glucosidase-3.2.1.21
68379N-acetyl-beta-glucosaminidase-3.2.1.52
68379alpha-glucosidase-3.2.1.20
68379beta-galactosidase-3.2.1.23
68379beta-glucuronidase-3.2.1.31
68379pyrrolidonyl arylamidase+3.4.19.3
68379pyrazinamidase+3.5.1.B15
123512oxidase-
123512beta-galactosidase-3.2.1.23
123512gelatinase-
123512amylase-
123512DNase-
123512caseinase-3.4.21.50
123512catalase+1.11.1.6
123512tween esterase-
123512gamma-glutamyltransferase-2.3.2.2
123512lecithinase-
123512lipase-
123512urease+3.5.1.5
68382lipase (C 14)-
68382leucine arylamidase+3.4.11.1
68382acid phosphatase+3.1.3.2
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase-3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase-3.2.1.20
68382beta-glucosidase-3.2.1.21
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API coryne

@refNITPYZPYRAPALbeta GURbeta GALalpha GLUbeta NAGESCUREGELGLURIBXYLMANMALLACSACGLYG
20011-+++/--------------+-

API zym

@refAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- FucosidaseControl
20011+++-+++++++--------
11565-+/-+/--+-+/---+----------
123512-++-+----++---------

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
123512-+-------------------------------------------------------------------------+--+---------+-------+--

Isolation, sampling and environmental information

isolation

@refsample type
11565soil
67770Soil
123512Environment, Soil

isolation source categories

  • Cat1: #Environmental
  • Cat2: #Terrestrial
  • Cat3: #Soil

Safety information

risk assessment

@refpathogenicity humanpathogenicity animalbiosafety levelbiosafety level comment
11565yes, in single casesyes, in single cases1Risk group (German classification)
200111German classification
1235121Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218M.thermoresistible 16S ribosomal RNAM295701359ena1797
20218M.thermoresistible 16S ribosomal RNA, partX556021464ena1797
20218Mycobacterium thermoresistibile strain CIP 105390 16S ribosomal RNA gene, partial sequenceAF547971547ena1797

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Mycolicibacterium thermoresistibile NCTC10409GCA_900187065completencbi1797
66792Mycobacterium thermoresistibile ATCC 195271078020.3wgspatric1078020
66792Mycobacterium thermoresistibile strain JCM63621797.3wgspatric1797
66792Mycobacterium thermoresistibile strain NCTC104091797.4completepatric1797
66792Mycolicibacterium thermoresistibile ATCC 195272519103087draftimg1078020
66792Mycolicibacterium thermoresistibile NCTC 104092773858053completeimg1797
67770Mycolicibacterium thermoresistibile ATCC 19527GCA_000234585contigncbi1078020
67770Mycolicibacterium thermoresistibile JCM6362GCA_001570505scaffoldncbi1797
66792Mycolicibacterium thermoresistibile DSM 44167GCA_025822325scaffoldncbi1797

GC content

  • @ref: 67770
  • GC-content: 69
  • method: genome sequence analysis

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingyes65no
motileno95.456no
flagellatedno99.055no
gram-positiveyes88.095no
anaerobicno99.183no
aerobicyes88.021no
halophileno82.681no
spore-formingno86.638no
thermophileno98.158no
glucose-utilyes82.053no
glucose-fermentno90.727no

External links

@ref: 11565

culture collection no.: DSM 44167, ATCC 19527, JCM 6392, NCTC 10409, JCM 6362, CCUG 28008, CCUG 41353, CIP 105390

straininfo link

  • @ref: 77824
  • straininfo: 36991

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
11565Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 44167)https://www.dsmz.de/collection/catalogue/details/culture/DSM-44167
20011Wink, J.http://www.dsmz.de/microorganisms/wink_pdf/DSM44167.pdfCompendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
36375Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/17404
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
66794Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg10.1093/nar/gkaa1025BRENDA, the ELIXIR core data resource in 2021: new developments and updatesNucleic Acids Res. 49: D498-D508 202033211880
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68379Automatically annotated from API Coryne
68382Automatically annotated from API zym
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
77824Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID36991.1StrainInfo: A central database for resolving microbial strain identifiers
123512Curators of the CIPCollection of Institut Pasteur (CIP 105390)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20105390