Strain identifier
BacDive ID: 8375
Type strain: 
Species: Mycobacterium neoaurum
Strain Designation: 309
Strain history: <- I. Tarnok, SN 2600 <- M. Tsukamura, 309 (M. aurum)
NCBI tax ID(s): 1795 (species)
version 9.3 (current version)
General
@ref: 11124
BacDive-ID: 8375
DSM-Number: 43536
keywords: genome sequence, 16S sequence, Bacteria, aerobe, mesophilic, Gram-positive
description: Mycobacterium neoaurum 309 is an aerobe, mesophilic, Gram-positive bacterium of the family Mycobacteriaceae.
NCBI tax id
- NCBI tax id: 1795
- Matching level: species
strain history
- @ref: 11124
- history: <- I. Tarnok, SN 2600 <- M. Tsukamura, 309 (M. aurum)
doi: 10.13145/bacdive8375.20250331.9.3
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/actinomycetota
- domain: Bacteria
- phylum: Actinomycetota
- class: Actinomycetes
- order: Mycobacteriales
- family: Mycobacteriaceae
- genus: Mycobacterium
- species: Mycobacterium neoaurum
- full scientific name: Mycobacterium neoaurum Tsukamura 1972 (Approved Lists 1980)
synonyms
- @ref: 20215
- synonym: Mycolicibacterium neoaurum
@ref: 11124
domain: Bacteria
phylum: Actinobacteria
class: Actinobacteria
order: Mycobacteriales
family: Mycobacteriaceae
genus: Mycobacterium
species: Mycobacterium neoaurum
full scientific name: Mycobacterium neoaurum Tsukamura 1972
strain designation: 309
type strain: no
Morphology
cell morphology
- @ref: 125438
- gram stain: positive
- confidence: 91.803
Culture and growth conditions
culture medium
| @ref | name | growth | link | composition |
|---|---|---|---|---|
| 11124 | MIDDLEBROOK MEDIUM (DSMZ Medium 645) | yes | https://mediadive.dsmz.de/medium/645 | Name: MIDDLEBROOK MEDIUM (DSMZ Medium 645) Composition: Bacto Middlebrook 7H10 agar 20.9945 g/l Glycerol Distilled water |
| 11124 | LÖWENSTEIN-JENSEN MEDIUM (DSMZ Medium 354) | yes | https://mediadive.dsmz.de/medium/354 | Name: LÖWENSTEIN-JENSEN MEDIUM (DSMZ Medium 354) Composition: Potato flour 18.6104 g/l L-Asparagin 2.23325 g/l KH2PO4 1.55087 g/l Mg-citrate 0.372208 g/l Malachite green 0.248139 g/l MgSO4 0.148883 g/l Glycerol Fresh egg mixture Distilled water |
culture temp
- @ref: 11124
- growth: positive
- type: growth
- temperature: 37
Physiology and metabolism
oxygen tolerance
- @ref: 125438
- oxygen tolerance: aerobe
- confidence: 90.843
metabolite utilization
| @ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
|---|---|---|---|---|
| 68369 | 25115 | malate | + | assimilation |
| 68369 | 27689 | decanoate | - | assimilation |
| 68369 | 24265 | gluconate | + | assimilation |
| 68369 | 17306 | maltose | - | assimilation |
| 68369 | 59640 | N-acetylglucosamine | - | assimilation |
| 68369 | 16899 | D-mannitol | + | assimilation |
| 68369 | 16024 | D-mannose | - | assimilation |
| 68369 | 30849 | L-arabinose | - | assimilation |
| 68369 | 5291 | gelatin | - | hydrolysis |
| 68369 | 16199 | urea | + | hydrolysis |
| 68369 | 29016 | arginine | - | hydrolysis |
| 68369 | 17634 | D-glucose | - | fermentation |
| 68369 | 27897 | tryptophan | - | energy source |
metabolite production
- @ref: 68369
- Chebi-ID: 35581
- metabolite: indole
- production: no
metabolite tests
- @ref: 68369
- Chebi-ID: 35581
- metabolite: indole
- indole test: -
enzymes
| @ref | value | activity | ec |
|---|---|---|---|
| 68382 | alpha-fucosidase | - | 3.2.1.51 |
| 68382 | N-acetyl-beta-glucosaminidase | - | 3.2.1.52 |
| 68382 | beta-glucosidase | + | 3.2.1.21 |
| 68382 | beta-glucuronidase | - | 3.2.1.31 |
| 68382 | alpha-galactosidase | - | 3.2.1.22 |
| 68382 | beta-galactosidase | - | 3.2.1.23 |
| 68382 | acid phosphatase | + | 3.1.3.2 |
| 68382 | cystine arylamidase | + | 3.4.11.3 |
| 68382 | leucine arylamidase | + | 3.4.11.1 |
| 68382 | lipase (C 14) | + | |
| 68382 | esterase lipase (C 8) | + | |
| 68382 | alkaline phosphatase | + | 3.1.3.1 |
| 68369 | gelatinase | - | |
| 68369 | urease | + | 3.5.1.5 |
| 68369 | arginine dihydrolase | - | 3.5.3.6 |
API zym
| @ref | Control | Alkaline phosphatase | Esterase | Esterase Lipase | Lipase | Leucine arylamidase | Valine arylamidase | Cystine arylamidase | Trypsin | alpha- Chymotrypsin | Acid phosphatase | Naphthol-AS-BI-phosphohydrolase | alpha- Galactosidase | beta- Galactosidase | beta- Glucuronidase | alpha- Glucosidase | beta- Glucosidase | N-acetyl-beta- glucosaminidase | alpha- Mannosidase | alpha- Fucosidase |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 11124 | - | + | +/- | + | + | + | +/- | + | +/- | +/- | + | +/- | - | - | - | +/- | + | - | +/- | - |
API 20NE
| @ref | NO3 | TRP | GLU_ Ferm | ADH Arg | URE | ESC | GEL | PNPG | GLU_ Assim | ARA | MNE | MAN | NAG | MAL | GNT | CAP | ADI | MLT | CIT | PAC |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 11124 | +/- | - | - | - | + | +/- | - | - | +/- | - | - | + | - | - | + | - | +/- | + | +/- | - |
Safety information
risk assessment
- @ref: 11124
- biosafety level: 2
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
- @ref: 20218
- description: Mycobacterium neoaurum strain ATCC 23072 16S ribosomal RNA gene, partial sequence
- accession: FJ172309
- length: 1453
- database: nuccore
- NCBI tax ID: 1795
Genome sequences
- @ref: 66792
- description: Mycolicibacterium neoaurum DSM 43536
- accession: GCA_027627515
- assembly level: chromosome
- database: ncbi
- NCBI tax ID: 1795
Genome-based predictions
predictions
| @ref | model | trait | description | prediction | confidence | training_data |
|---|---|---|---|---|---|---|
| 125438 | gram-positive | gram-positive | Positive reaction to Gram-staining | yes | 91.803 | no |
| 125438 | anaerobic | anaerobic | Ability to grow under anoxygenic conditions (including facultative anaerobes) | no | 97.983 | no |
| 125438 | aerobic | aerobic | Ability to grow under oxygenic conditions (including facultative aerobes) | yes | 90.843 | no |
| 125438 | spore-forming | spore-forming | Ability to form endo- or exospores | no | 58.598 | no |
| 125438 | thermophile | thermophilic | Ability to grow at temperatures above or equal to 45°C | no | 97 | yes |
| 125438 | motile2+ | flagellated | Ability to perform flagellated movement | no | 88 | no |
External links
@ref: 11124
culture collection no.: DSM 43536, ATCC 23072, ATCC 25790, NCTC 10440, SN 2600
straininfo link
- @ref: 77774
- straininfo: 37893
literature
- topic: Metabolism
- Pubmed-ID: 2362446
- title: Formation of progesterone and 1-dehydroprogesterone from cholesterol in fermentation cultures of Mycobacterium aurum.
- authors: Horhold C, Bohme KH
- journal: J Steroid Biochem
- DOI: 10.1016/0022-4731(90)90128-f
- year: 1990
- mesh: Cholesterol/*metabolism, *Fermentation, Mycobacterium/growth & development/*metabolism, Progesterone/*analogs & derivatives/*biosynthesis
Reference
| @id | authors | catalogue | doi/url | title | journal | pubmed |
|---|---|---|---|---|---|---|
| 11124 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 43536) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-43536 | |||
| 20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |||
| 20218 | Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P. | 10.1016/j.syapm.2013.11.002 | StrainInfo introduces electronic passports for microorganisms. | Syst Appl Microbiol. 37: 42-50 2014 | 24321274 | |
| 66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |||
| 68369 | Automatically annotated from API 20NE | |||||
| 68382 | Automatically annotated from API zym | |||||
| 77774 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID37893.1 | StrainInfo: A central database for resolving microbial strain identifiers | |||
| 125438 | Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann | 10.1101/2024.08.12.607695 | Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets |