Strain identifier

BacDive ID: 8373

Type strain: Yes

Species: Mycobacterium szulgai

Strain Designation: 25932, 14 024 0001

Strain history: CIP <- 1995, V. Vincent, Inst. Pasteur, Paris, France: strain 14 024 0001 <- ATCC <- J. Marks: strain 25932

NCBI tax ID(s): 1787 (species)

For citation purpose refer to the digital object identifier (doi) of the current version.
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General

@ref: 11564

BacDive-ID: 8373

DSM-Number: 44166

keywords: genome sequence, 16S sequence, Bacteria, aerobe, mesophilic, Gram-positive, human pathogen

description: Mycobacterium szulgai 25932 is an aerobe, mesophilic, Gram-positive human pathogen that was isolated from cervical adenitis.

NCBI tax id

  • NCBI tax id: 1787
  • Matching level: species

strain history

@refhistory
11564<- ATCC; ATCC 35799 <- TMC; TMC 1328 <- NCTC; NCTC 10831 <- J. Marks; 25932
67770M. Tsukamura SZ12002 <-- J. Marks 25932.
123404CIP <- 1995, V. Vincent, Inst. Pasteur, Paris, France: strain 14 024 0001 <- ATCC <- J. Marks: strain 25932

doi: 10.13145/bacdive8373.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Mycobacteriales
  • family: Mycobacteriaceae
  • genus: Mycobacterium
  • species: Mycobacterium szulgai
  • full scientific name: Mycobacterium szulgai Marks et al. 1972 (Approved Lists 1980)

@ref: 11564

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Mycobacteriaceae

genus: Mycobacterium

species: Mycobacterium szulgai

full scientific name: Mycobacterium szulgai Marks et al. 1972 emend. Nouioui et al. 2018

strain designation: 25932, 14 024 0001

type strain: yes

Morphology

cell morphology

@refgram stainconfidencecell shapemotility
69480positive100
123404positiverod-shapedno

colony morphology

@refcolony colorincubation periodmedium used
20010Zinc yellow (1018)10-14 days5006
20010Zinc yellow (1018)10-14 daysMB7H10
20010Zinc yellow (1018)10-14 daysMB7H11
538027 days

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
11564MIDDLEBROOK MEDIUM (DSMZ Medium 645)yeshttps://mediadive.dsmz.de/medium/645Name: MIDDLEBROOK MEDIUM (DSMZ Medium 645) Composition: Bacto Middlebrook 7H10 agar 20.9945 g/l Glycerol Distilled water
20010MB7H10yesName: Middlebrook 7H10 (MB7H10) Broth Base Composition: amount in percent Middlebrook 7H10 Agar 1.9% Glycerol 0.5% OADC-Enrichment in ml (NL5633) 10ml Comments: Add broth base and glycerol to ddh2O. Adjust volumes. Autoclave. Cool to 42°C. Add OADC-stock. Sterilisation: 20 minutes Temperature:121°C PH before: PH after: Biological details Culture type: suitable for: Mycobacteria OADC stock solution old broth-name: OADC-Stock Composition: Bovine Serum Albumin, fraction V 5 % 1% Oleic Acid in 0.2N NaOH 5ml Glucose 2 % NaCl 0.85 % Comments: Melt 1% Oleic Acid in 65°C water bath. Dissolve all ingredients in 700ml of ddH2O. Adjust volume to 1L. Filter sterilize and store at 4°C. -To make 1% Oleic Acid in 0.2N NaOH: Dissolve 5g oleic acid (ampule) in 500ml 0.2N NaOH (300ml ddH2O + 20ml NaOH + adjust volume to 500ml). Heat solution to 55°C for oleic acid to melt. Store at -20°C in 50ml aliquots. There is no need to sterilize this. Sterilisation: 20 minutes Temperature:121°C PH before: PH after: Biological details Culture type: suitable for: Mycobacteria
20010MB7H11yesName: Middlebrook 7H11 (MB7H11) Broth Base Composition: Middlebrook 7H11 Agar 2% Glycerol 0.5% OADC-Enrichment 10ml Comments: Add broth base and glycerol to ddH2O. Adjust volumes. Autoclave. Cool to 42°C. Add OADC-Stock. Sterilisation: 20 minutes Temperature:121°C PH before: PH after: Biological details Culture type: suitable for: Mycobacteria OADC stock solution old broth-name: OADC-Stock Composition: Bovine Serum Albumin, fraction V 5 % 1% Oleic Acid in 0.2N NaOH 5ml Glucose 2 % NaCl 0.85 % Comments: Melt 1% Oleic Acid in 65°C water bath. Dissolve all ingredients in 700ml of ddH2O. Adjust volume to 1L. Filter sterilize and store at 4°C. -To make 1% Oleic Acid in 0.2N NaOH: Dissolve 5g oleic acid (ampule) in 500ml 0.2N NaOH (300ml ddH2O + 20ml NaOH + adjust volume to 500ml). Heat solution to 55°C for oleic acid to melt. Store at -20°C in 50ml aliquots. There is no need to sterilize this. Sterilisation: 20 minutes Temperature:121°C PH before: PH after: Biological details Culture type: suitable for: Mycobacteria
200105006yesMedium: 5006 Name: Composition (g/l) Sucrose 3,0 Dextrin 15,0 Meat extract 1,0 Yeast extract 2,0 Tryptone soy broth (Oxoid) 5,0 NaCl 0,5 K2HPO4 0,5 MgSO4 x 7 H2O 0,5 FeSO4 x 7 H2O 0,01 Agar 20,0 Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7,3 Usage: Maintenance Organisms: All Actinomycetes
36461MEDIUM 55 - for Mycobacteriumyes
123404CIP Medium 55yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=55

culture temp

@refgrowthtypetemperaturerange
20010positiveoptimum37mesophilic
11564positivegrowth37mesophilic
36461positivegrowth37mesophilic
53802positivegrowth37mesophilic
67770positivegrowth37mesophilic
123404positivegrowth30-37mesophilic
123404nogrowth10psychrophilic
123404nogrowth45thermophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 53802
  • oxygen tolerance: aerobe

spore formation

  • @ref: 69480
  • spore formation: no
  • confidence: 99.84

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
12340416947citrate-carbon source
1234044853esculin-hydrolysis
123404606565hippurate+hydrolysis
12340417632nitrate-reduction
12340416301nitrite-reduction
683794853esculin+hydrolysis
6837916199urea+hydrolysis
683795291gelatin+hydrolysis
6837917634D-glucose+fermentation
6837916988D-ribose-fermentation
6837965327D-xylose-fermentation
6837916899D-mannitol-fermentation
6837917306maltose-fermentation
6837917716lactose-fermentation
6837917992sucrose-fermentation
6837928087glycogen-fermentation

metabolite production

  • @ref: 123404
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

metabolite tests

  • @ref: 123404
  • Chebi-ID: 15688
  • metabolite: acetoin
  • voges-proskauer-test: -

enzymes

@refvalueactivityec
68379gelatinase+
68379urease+3.5.1.5
68379beta-glucosidase+3.2.1.21
68379N-acetyl-beta-glucosaminidase-3.2.1.52
68379alpha-glucosidase+3.2.1.20
68379beta-galactosidase-3.2.1.23
68379beta-glucuronidase-3.2.1.31
68379alkaline phosphatase+3.1.3.1
68379pyrrolidonyl arylamidase+3.4.19.3
68379pyrazinamidase+3.5.1.B15
123404oxidase-
123404beta-galactosidase-3.2.1.23
123404alcohol dehydrogenase-1.1.1.1
123404gelatinase-
123404catalase+1.11.1.6
123404lysine decarboxylase-4.1.1.18
123404ornithine decarboxylase-4.1.1.17
123404tryptophan deaminase-
123404urease+3.5.1.5
68382alkaline phosphatase+3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)+
68382leucine arylamidase+3.4.11.1
68382cystine arylamidase-3.4.11.3
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase-3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382beta-glucosidase-3.2.1.21
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API coryne

@refNITPYZPYRAPALbeta GURbeta GALalpha GLUbeta NAGESCUREGELGLURIBXYLMANMALLACSACGLYG
20010+/-+++--+-++++-------

API zym

@refAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- FucosidaseControl
20010+++-+---+++---+----
123404++++++-+-++---------

Isolation, sampling and environmental information

isolation

@refsample type
11564cervical adenitis
53802Cervical adenitis
67770Lymph node from a body suffering from cervical adenitis

isolation source categories

Cat1Cat2Cat3
#Infection#Inflammation
#Host Body-Site#Oral cavity and airways#Throat
#Host Body-Site#Organ#Lymph node

taxonmaps

  • @ref: 69479
  • File name: preview.99_75.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_10;96_37;97_39;98_43;99_75&stattab=map
  • Last taxonomy: Mycobacterium
  • 16S sequence: X52926
  • Sequence Identity:
  • Total samples: 1520
  • soil counts: 881
  • aquatic counts: 382
  • animal counts: 203
  • plant counts: 54

Safety information

risk assessment

@refpathogenicity humanpathogenicity animalbiosafety levelbiosafety level comment
11564yesyes2Risk group (German classification)
200102German classification
1234042Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Mycobacterium szulgai strain ATCC 35799 16S ribosomal RNA gene, partial sequenceKC9512781486ena1787
20218M.szulgai 16S ribosomal RNA geneM59274146ena1787
20218M.szulgai 16S ribosomal rRNAM616651379ena1787
20218Mycobacterium szulgai 16S rRNA geneX529261462ena1787
20218M.szulgai internal transcribed spacer between 16S and 23S rRNA, genomic sequenceX99220275ena1787
20218Mycobacterium szulgai strain CIP 104532 16S ribosomal RNA gene, partial sequenceAF547969554ena1787
20218Mycobacterium szulgai DNA for 16S rRNA, internal transcribed spacer, 23S rRNAAB026704433ena1787

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Mycobacterium szulgai strain DSM 441661787.5wgspatric1787
66792Mycobacterium szulgai DSM 441662865439911draftimg1787
67770Mycobacterium szulgai DSM 44166GCA_002116635contigncbi1787
66792Mycobacterium szulgai DSM 44166GCA_025821385scaffoldncbi1787

GC content

  • @ref: 67770
  • GC-content: 65.8
  • method: genome sequence analysis

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno87no
motileno95.297no
gram-positiveyes86.491no
anaerobicno99.004yes
aerobicyes90.113yes
halophileno93.984no
spore-formingno85.925no
thermophileno98.725yes
glucose-utilyes62.856no
flagellatedno98.812no
glucose-fermentno88.926yes

External links

@ref: 11564

culture collection no.: DSM 44166, ATCC 35799, JCM 6383, NCTC 10831, PCM 1876, TMC 1328, CCUG 37675, CIP 104532, KCTC 9520

straininfo link

  • @ref: 77772
  • straininfo: 41299

literature

topicPubmed-IDtitleauthorsjournalyearmeshtopic2DOI
Phylogeny12235845[Microplate hybridization methods of reduced unspecific reassociation considering delta Tm--comparison between relative relatedness of DNA and key characters for Mycobacterium szulgai].Fukasawa YKekkaku2002DNA, Bacterial/*analysis, Nontuberculous Mycobacteria/genetics/*isolation & purification, Nucleic Acid Hybridization/*methods, TemperatureEnzymology
Phylogeny26420689Mycobacterium angelicum sp. nov., a non-chromogenic, slow-growing species isolated from fish and related to Mycobacterium szulgai.Pourahmad F, Pate M, Ocepek M, Borroni E, Cabibbe AM, Capitolo E, Cittaro D, Frizzera E, Jencic V, Mariottini A, Marumo K, Vaggelli G, Cirillo DM, Tortoli EInt J Syst Evol Microbiol2015Animals, Bacterial Typing Techniques, Cichlids/*microbiology, DNA, Bacterial/genetics, Fresh Water, Japan, Molecular Sequence Data, Mycobacterium/*classification/genetics/isolation & purification, Nontuberculous Mycobacteria, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNAGenetics10.1099/ijsem.0.000642

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
11564Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 44166)https://www.dsmz.de/collection/catalogue/details/culture/DSM-44166
20010Wink, J.http://www.dsmz.de/microorganisms/wink_pdf/DSM44166.pdfCompendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
36461Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/16451
53802Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 37675)https://www.ccug.se/strain?id=37675
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68379Automatically annotated from API Coryne
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
77772Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID41299.1StrainInfo: A central database for resolving microbial strain identifiers
123404Curators of the CIPCollection of Institut Pasteur (CIP 104532)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20104532