Strain identifier
BacDive ID: 8373
Type strain:
Species: Mycobacterium szulgai
Strain Designation: 25932, 14 024 0001
Strain history: CIP <- 1995, V. Vincent, Inst. Pasteur, Paris, France: strain 14 024 0001 <- ATCC <- J. Marks: strain 25932
NCBI tax ID(s): 1787 (species)
General
@ref: 11564
BacDive-ID: 8373
DSM-Number: 44166
keywords: genome sequence, 16S sequence, Bacteria, aerobe, mesophilic, Gram-positive, human pathogen
description: Mycobacterium szulgai 25932 is an aerobe, mesophilic, Gram-positive human pathogen that was isolated from cervical adenitis.
NCBI tax id
- NCBI tax id: 1787
- Matching level: species
strain history
@ref | history |
---|---|
11564 | <- ATCC; ATCC 35799 <- TMC; TMC 1328 <- NCTC; NCTC 10831 <- J. Marks; 25932 |
67770 | M. Tsukamura SZ12002 <-- J. Marks 25932. |
123404 | CIP <- 1995, V. Vincent, Inst. Pasteur, Paris, France: strain 14 024 0001 <- ATCC <- J. Marks: strain 25932 |
doi: 10.13145/bacdive8373.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/actinomycetota
- domain: Bacteria
- phylum: Actinomycetota
- class: Actinomycetes
- order: Mycobacteriales
- family: Mycobacteriaceae
- genus: Mycobacterium
- species: Mycobacterium szulgai
- full scientific name: Mycobacterium szulgai Marks et al. 1972 (Approved Lists 1980)
@ref: 11564
domain: Bacteria
phylum: Actinobacteria
class: Actinobacteria
order: Actinomycetales
family: Mycobacteriaceae
genus: Mycobacterium
species: Mycobacterium szulgai
full scientific name: Mycobacterium szulgai Marks et al. 1972 emend. Nouioui et al. 2018
strain designation: 25932, 14 024 0001
type strain: yes
Morphology
cell morphology
@ref | gram stain | confidence | cell shape | motility |
---|---|---|---|---|
69480 | positive | 100 | ||
123404 | positive | rod-shaped | no |
colony morphology
@ref | colony color | incubation period | medium used |
---|---|---|---|
20010 | Zinc yellow (1018) | 10-14 days | 5006 |
20010 | Zinc yellow (1018) | 10-14 days | MB7H10 |
20010 | Zinc yellow (1018) | 10-14 days | MB7H11 |
53802 | 7 days |
Culture and growth conditions
culture medium
@ref | name | growth | link | composition |
---|---|---|---|---|
11564 | MIDDLEBROOK MEDIUM (DSMZ Medium 645) | yes | https://mediadive.dsmz.de/medium/645 | Name: MIDDLEBROOK MEDIUM (DSMZ Medium 645) Composition: Bacto Middlebrook 7H10 agar 20.9945 g/l Glycerol Distilled water |
20010 | MB7H10 | yes | Name: Middlebrook 7H10 (MB7H10) Broth Base Composition: amount in percent Middlebrook 7H10 Agar 1.9% Glycerol 0.5% OADC-Enrichment in ml (NL5633) 10ml Comments: Add broth base and glycerol to ddh2O. Adjust volumes. Autoclave. Cool to 42°C. Add OADC-stock. Sterilisation: 20 minutes Temperature:121°C PH before: PH after: Biological details Culture type: suitable for: Mycobacteria OADC stock solution old broth-name: OADC-Stock Composition: Bovine Serum Albumin, fraction V 5 % 1% Oleic Acid in 0.2N NaOH 5ml Glucose 2 % NaCl 0.85 % Comments: Melt 1% Oleic Acid in 65°C water bath. Dissolve all ingredients in 700ml of ddH2O. Adjust volume to 1L. Filter sterilize and store at 4°C. -To make 1% Oleic Acid in 0.2N NaOH: Dissolve 5g oleic acid (ampule) in 500ml 0.2N NaOH (300ml ddH2O + 20ml NaOH + adjust volume to 500ml). Heat solution to 55°C for oleic acid to melt. Store at -20°C in 50ml aliquots. There is no need to sterilize this. Sterilisation: 20 minutes Temperature:121°C PH before: PH after: Biological details Culture type: suitable for: Mycobacteria | |
20010 | MB7H11 | yes | Name: Middlebrook 7H11 (MB7H11) Broth Base Composition: Middlebrook 7H11 Agar 2% Glycerol 0.5% OADC-Enrichment 10ml Comments: Add broth base and glycerol to ddH2O. Adjust volumes. Autoclave. Cool to 42°C. Add OADC-Stock. Sterilisation: 20 minutes Temperature:121°C PH before: PH after: Biological details Culture type: suitable for: Mycobacteria OADC stock solution old broth-name: OADC-Stock Composition: Bovine Serum Albumin, fraction V 5 % 1% Oleic Acid in 0.2N NaOH 5ml Glucose 2 % NaCl 0.85 % Comments: Melt 1% Oleic Acid in 65°C water bath. Dissolve all ingredients in 700ml of ddH2O. Adjust volume to 1L. Filter sterilize and store at 4°C. -To make 1% Oleic Acid in 0.2N NaOH: Dissolve 5g oleic acid (ampule) in 500ml 0.2N NaOH (300ml ddH2O + 20ml NaOH + adjust volume to 500ml). Heat solution to 55°C for oleic acid to melt. Store at -20°C in 50ml aliquots. There is no need to sterilize this. Sterilisation: 20 minutes Temperature:121°C PH before: PH after: Biological details Culture type: suitable for: Mycobacteria | |
20010 | 5006 | yes | Medium: 5006 Name: Composition (g/l) Sucrose 3,0 Dextrin 15,0 Meat extract 1,0 Yeast extract 2,0 Tryptone soy broth (Oxoid) 5,0 NaCl 0,5 K2HPO4 0,5 MgSO4 x 7 H2O 0,5 FeSO4 x 7 H2O 0,01 Agar 20,0 Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7,3 Usage: Maintenance Organisms: All Actinomycetes | |
36461 | MEDIUM 55 - for Mycobacterium | yes | ||
123404 | CIP Medium 55 | yes | https://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=55 |
culture temp
@ref | growth | type | temperature | range |
---|---|---|---|---|
20010 | positive | optimum | 37 | mesophilic |
11564 | positive | growth | 37 | mesophilic |
36461 | positive | growth | 37 | mesophilic |
53802 | positive | growth | 37 | mesophilic |
67770 | positive | growth | 37 | mesophilic |
123404 | positive | growth | 30-37 | mesophilic |
123404 | no | growth | 10 | psychrophilic |
123404 | no | growth | 45 | thermophilic |
Physiology and metabolism
oxygen tolerance
- @ref: 53802
- oxygen tolerance: aerobe
spore formation
- @ref: 69480
- spore formation: no
- confidence: 99.84
metabolite utilization
@ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
---|---|---|---|---|
123404 | 16947 | citrate | - | carbon source |
123404 | 4853 | esculin | - | hydrolysis |
123404 | 606565 | hippurate | + | hydrolysis |
123404 | 17632 | nitrate | - | reduction |
123404 | 16301 | nitrite | - | reduction |
68379 | 4853 | esculin | + | hydrolysis |
68379 | 16199 | urea | + | hydrolysis |
68379 | 5291 | gelatin | + | hydrolysis |
68379 | 17634 | D-glucose | + | fermentation |
68379 | 16988 | D-ribose | - | fermentation |
68379 | 65327 | D-xylose | - | fermentation |
68379 | 16899 | D-mannitol | - | fermentation |
68379 | 17306 | maltose | - | fermentation |
68379 | 17716 | lactose | - | fermentation |
68379 | 17992 | sucrose | - | fermentation |
68379 | 28087 | glycogen | - | fermentation |
metabolite production
- @ref: 123404
- Chebi-ID: 35581
- metabolite: indole
- production: no
metabolite tests
- @ref: 123404
- Chebi-ID: 15688
- metabolite: acetoin
- voges-proskauer-test: -
enzymes
@ref | value | activity | ec |
---|---|---|---|
68379 | gelatinase | + | |
68379 | urease | + | 3.5.1.5 |
68379 | beta-glucosidase | + | 3.2.1.21 |
68379 | N-acetyl-beta-glucosaminidase | - | 3.2.1.52 |
68379 | alpha-glucosidase | + | 3.2.1.20 |
68379 | beta-galactosidase | - | 3.2.1.23 |
68379 | beta-glucuronidase | - | 3.2.1.31 |
68379 | alkaline phosphatase | + | 3.1.3.1 |
68379 | pyrrolidonyl arylamidase | + | 3.4.19.3 |
68379 | pyrazinamidase | + | 3.5.1.B15 |
123404 | oxidase | - | |
123404 | beta-galactosidase | - | 3.2.1.23 |
123404 | alcohol dehydrogenase | - | 1.1.1.1 |
123404 | gelatinase | - | |
123404 | catalase | + | 1.11.1.6 |
123404 | lysine decarboxylase | - | 4.1.1.18 |
123404 | ornithine decarboxylase | - | 4.1.1.17 |
123404 | tryptophan deaminase | - | |
123404 | urease | + | 3.5.1.5 |
68382 | alkaline phosphatase | + | 3.1.3.1 |
68382 | esterase (C 4) | + | |
68382 | esterase lipase (C 8) | + | |
68382 | leucine arylamidase | + | 3.4.11.1 |
68382 | cystine arylamidase | - | 3.4.11.3 |
68382 | acid phosphatase | + | 3.1.3.2 |
68382 | naphthol-AS-BI-phosphohydrolase | + | |
68382 | alpha-galactosidase | - | 3.2.1.22 |
68382 | beta-galactosidase | - | 3.2.1.23 |
68382 | beta-glucuronidase | - | 3.2.1.31 |
68382 | beta-glucosidase | - | 3.2.1.21 |
68382 | N-acetyl-beta-glucosaminidase | - | 3.2.1.52 |
68382 | alpha-mannosidase | - | 3.2.1.24 |
68382 | alpha-fucosidase | - | 3.2.1.51 |
API coryne
@ref | NIT | PYZ | PYRA | PAL | beta GUR | beta GAL | alpha GLU | beta NAG | ESC | URE | GEL | GLU | RIB | XYL | MAN | MAL | LAC | SAC | GLYG |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
20010 | +/- | + | + | + | - | - | + | - | + | + | + | + | - | - | - | - | - | - | - |
API zym
@ref | Alkaline phosphatase | Esterase | Esterase Lipase | Lipase | Leucine arylamidase | Valine arylamidase | Cystine arylamidase | Trypsin | alpha- Chymotrypsin | Acid phosphatase | Naphthol-AS-BI-phosphohydrolase | alpha- Galactosidase | beta- Galactosidase | beta- Glucuronidase | alpha- Glucosidase | beta- Glucosidase | N-acetyl-beta- glucosaminidase | alpha- Mannosidase | alpha- Fucosidase | Control |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
20010 | + | + | + | - | + | - | - | - | + | + | + | - | - | - | + | - | - | - | - | |
123404 | + | + | + | + | + | + | - | + | - | + | + | - | - | - | - | - | - | - | - | - |
Isolation, sampling and environmental information
isolation
@ref | sample type |
---|---|
11564 | cervical adenitis |
53802 | Cervical adenitis |
67770 | Lymph node from a body suffering from cervical adenitis |
isolation source categories
Cat1 | Cat2 | Cat3 |
---|---|---|
#Infection | #Inflammation | |
#Host Body-Site | #Oral cavity and airways | #Throat |
#Host Body-Site | #Organ | #Lymph node |
taxonmaps
- @ref: 69479
- File name: preview.99_75.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_10;96_37;97_39;98_43;99_75&stattab=map
- Last taxonomy: Mycobacterium
- 16S sequence: X52926
- Sequence Identity:
- Total samples: 1520
- soil counts: 881
- aquatic counts: 382
- animal counts: 203
- plant counts: 54
Safety information
risk assessment
@ref | pathogenicity human | pathogenicity animal | biosafety level | biosafety level comment |
---|---|---|---|---|
11564 | yes | yes | 2 | Risk group (German classification) |
20010 | 2 | German classification | ||
123404 | 2 | Risk group (French classification) |
Sequence information
16S sequences
@ref | description | accession | length | database | NCBI tax ID |
---|---|---|---|---|---|
20218 | Mycobacterium szulgai strain ATCC 35799 16S ribosomal RNA gene, partial sequence | KC951278 | 1486 | ena | 1787 |
20218 | M.szulgai 16S ribosomal RNA gene | M59274 | 146 | ena | 1787 |
20218 | M.szulgai 16S ribosomal rRNA | M61665 | 1379 | ena | 1787 |
20218 | Mycobacterium szulgai 16S rRNA gene | X52926 | 1462 | ena | 1787 |
20218 | M.szulgai internal transcribed spacer between 16S and 23S rRNA, genomic sequence | X99220 | 275 | ena | 1787 |
20218 | Mycobacterium szulgai strain CIP 104532 16S ribosomal RNA gene, partial sequence | AF547969 | 554 | ena | 1787 |
20218 | Mycobacterium szulgai DNA for 16S rRNA, internal transcribed spacer, 23S rRNA | AB026704 | 433 | ena | 1787 |
Genome sequences
@ref | description | accession | assembly level | database | NCBI tax ID |
---|---|---|---|---|---|
66792 | Mycobacterium szulgai strain DSM 44166 | 1787.5 | wgs | patric | 1787 |
66792 | Mycobacterium szulgai DSM 44166 | 2865439911 | draft | img | 1787 |
67770 | Mycobacterium szulgai DSM 44166 | GCA_002116635 | contig | ncbi | 1787 |
66792 | Mycobacterium szulgai DSM 44166 | GCA_025821385 | scaffold | ncbi | 1787 |
GC content
- @ref: 67770
- GC-content: 65.8
- method: genome sequence analysis
Genome-based predictions
predictions
trait | prediction | confidence | training_data |
---|---|---|---|
spore-forming | no | 87 | no |
motile | no | 95.297 | no |
gram-positive | yes | 86.491 | no |
anaerobic | no | 99.004 | yes |
aerobic | yes | 90.113 | yes |
halophile | no | 93.984 | no |
spore-forming | no | 85.925 | no |
thermophile | no | 98.725 | yes |
glucose-util | yes | 62.856 | no |
flagellated | no | 98.812 | no |
glucose-ferment | no | 88.926 | yes |
External links
@ref: 11564
culture collection no.: DSM 44166, ATCC 35799, JCM 6383, NCTC 10831, PCM 1876, TMC 1328, CCUG 37675, CIP 104532, KCTC 9520
straininfo link
- @ref: 77772
- straininfo: 41299
literature
topic | Pubmed-ID | title | authors | journal | year | mesh | topic2 | DOI |
---|---|---|---|---|---|---|---|---|
Phylogeny | 12235845 | [Microplate hybridization methods of reduced unspecific reassociation considering delta Tm--comparison between relative relatedness of DNA and key characters for Mycobacterium szulgai]. | Fukasawa Y | Kekkaku | 2002 | DNA, Bacterial/*analysis, Nontuberculous Mycobacteria/genetics/*isolation & purification, Nucleic Acid Hybridization/*methods, Temperature | Enzymology | |
Phylogeny | 26420689 | Mycobacterium angelicum sp. nov., a non-chromogenic, slow-growing species isolated from fish and related to Mycobacterium szulgai. | Pourahmad F, Pate M, Ocepek M, Borroni E, Cabibbe AM, Capitolo E, Cittaro D, Frizzera E, Jencic V, Mariottini A, Marumo K, Vaggelli G, Cirillo DM, Tortoli E | Int J Syst Evol Microbiol | 2015 | Animals, Bacterial Typing Techniques, Cichlids/*microbiology, DNA, Bacterial/genetics, Fresh Water, Japan, Molecular Sequence Data, Mycobacterium/*classification/genetics/isolation & purification, Nontuberculous Mycobacteria, *Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA | Genetics | 10.1099/ijsem.0.000642 |
Reference
@id | authors | catalogue | doi/url | title | journal | pubmed |
---|---|---|---|---|---|---|
11564 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 44166) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-44166 | |||
20010 | Wink, J. | http://www.dsmz.de/microorganisms/wink_pdf/DSM44166.pdf | Compendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig | |||
20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |||
20218 | Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P. | 10.1016/j.syapm.2013.11.002 | StrainInfo introduces electronic passports for microorganisms. | Syst Appl Microbiol. 37: 42-50 2014 | 24321274 | |
36461 | Curators of the CIP | https://brclims.pasteur.fr/brcWeb/souche/detail/1/16451 | ||||
53802 | Curators of the CCUG | Culture Collection University of Gothenburg (CCUG) (CCUG 37675) | https://www.ccug.se/strain?id=37675 | |||
66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |||
67770 | Curators of the JCM | https://jcm.brc.riken.jp/en/ | ||||
68379 | Automatically annotated from API Coryne | |||||
68382 | Automatically annotated from API zym | |||||
69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | https://microbeatlas.org/ | MicrobeAtlas 1.0 beta | |||
69480 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |||
77772 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID41299.1 | StrainInfo: A central database for resolving microbial strain identifiers | |||
123404 | Curators of the CIP | Collection of Institut Pasteur (CIP 104532) | https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20104532 |