Strain identifier

BacDive ID: 8373

Type strain: Yes

Species: Mycobacterium szulgai

Culture col. no.: DSM 44166, ATCC 35799, JCM 6383, NCTC 10831, PCM 1876, TMC 1328, CCUG 37675

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General

@ref: 11564

BacDive-ID: 8373

DSM-Number: 44166

keywords: mesophilic, aerobe, Bacteria, 16S sequence, genome sequence, human pathogen

description: Mycobacterium szulgai DSM 44166 is an aerobe, mesophilic human pathogen that was isolated from cervical adenitis.

strain history: <- ATCC <- TMC <- NCTC <- J. Marks, 25932

doi: 10.13145/bacdive8373.20201210.5

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinobacteria
  • domain: Bacteria
  • phylum: Actinobacteria
  • class: Actinobacteria
  • order: Mycobacteriales
  • family: Mycobacteriaceae
  • genus: Mycobacterium
  • species: Mycobacterium szulgai
  • full scientific name: Mycobacterium szulgai Marks et al. 1972 (Approved Lists 1980)

@ref: 11564

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Mycobacteriaceae

genus: Mycobacterium

species: Mycobacterium szulgai

full scientific name: Mycobacterium szulgai Marks et al. 1972 emend. Nouioui et al. 2018

type strain: yes

Morphology

colony morphology

@refcolony colorincubation periodmedium used
20010Zinc yellow (1018)10-14 days5006
20010Zinc yellow (1018)10-14 daysMB7H10
20010Zinc yellow (1018)10-14 daysMB7H11
538027 days

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
11564LÖWENSTEIN-JENSEN MEDIUM (DSMZ Medium 354)yeshttps://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium354.pdf
11564MIDDLEBROOK MEDIUM (DSMZ Medium 645)yeshttps://www.dsmz.de/microorganisms/medium/pdf/DSMZ_Medium645.pdf
20010MB7H10yesName: Middlebrook 7H10 (MB7H10) Broth Base Composition: amount in percent Middlebrook 7H10 Agar 1.9% Glycerol 0.5% OADC-Enrichment in ml (NL5633) 10ml Comments: Add broth base and glycerol to ddh2O. Adjust volumes. Autoclave. Cool to 42°C. Add OADC-stock. Sterilisation: 20 minutes Temperature:121°C PH before: PH after: Biological details Culture type: suitable for: Mycobacteria OADC stock solution old broth-name: OADC-Stock Composition: Bovine Serum Albumin, fraction V 5 % 1% Oleic Acid in 0.2N NaOH 5ml Glucose 2 % NaCl 0.85 % Comments: Melt 1% Oleic Acid in 65°C water bath. Dissolve all ingredients in 700ml of ddH2O. Adjust volume to 1L. Filter sterilize and store at 4°C. -To make 1% Oleic Acid in 0.2N NaOH: Dissolve 5g oleic acid (ampule) in 500ml 0.2N NaOH (300ml ddH2O + 20ml NaOH + adjust volume to 500ml). Heat solution to 55°C for oleic acid to melt. Store at -20°C in 50ml aliquots. There is no need to sterilize this. Sterilisation: 20 minutes Temperature:121°C PH before: PH after: Biological details Culture type: suitable for: Mycobacteria
20010MB7H11yesName: Middlebrook 7H11 (MB7H11) Broth Base Composition: Middlebrook 7H11 Agar 2% Glycerol 0.5% OADC-Enrichment 10ml Comments: Add broth base and glycerol to ddH2O. Adjust volumes. Autoclave. Cool to 42°C. Add OADC-Stock. Sterilisation: 20 minutes Temperature:121°C PH before: PH after: Biological details Culture type: suitable for: Mycobacteria OADC stock solution old broth-name: OADC-Stock Composition: Bovine Serum Albumin, fraction V 5 % 1% Oleic Acid in 0.2N NaOH 5ml Glucose 2 % NaCl 0.85 % Comments: Melt 1% Oleic Acid in 65°C water bath. Dissolve all ingredients in 700ml of ddH2O. Adjust volume to 1L. Filter sterilize and store at 4°C. -To make 1% Oleic Acid in 0.2N NaOH: Dissolve 5g oleic acid (ampule) in 500ml 0.2N NaOH (300ml ddH2O + 20ml NaOH + adjust volume to 500ml). Heat solution to 55°C for oleic acid to melt. Store at -20°C in 50ml aliquots. There is no need to sterilize this. Sterilisation: 20 minutes Temperature:121°C PH before: PH after: Biological details Culture type: suitable for: Mycobacteria
200105006yesMedium: 5006 Name: Composition (g/l) Sucrose 3,0 Dextrin 15,0 Meat extract 1,0 Yeast extract 2,0 Tryptone soy broth (Oxoid) 5,0 NaCl 0,5 K2HPO4 0,5 MgSO4 x 7 H2O 0,5 FeSO4 x 7 H2O 0,01 Agar 20,0 Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7,3 Usage: Maintenance Organisms: All Actinomycetes
36461MEDIUM 55 - for Mycobacteriumyes

culture temp

@refgrowthtypetemperaturerange
20010positiveoptimum37mesophilic
11564positivegrowth37mesophilic
36461positivegrowth37mesophilic
53802positivegrowth37mesophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 53802
  • oxygen tolerance: aerobe

API coryne

@refNITPYZPYRAPALbeta GURbeta GALalpha GLUbeta NAGESCUREGELGLURIBXYLMANMALLACSACGLYG
20010+/-+++--+-++++-------

API zym

@refAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
20010+++-+---+++---+----

Isolation, sampling and environmental information

isolation

@refsample type
11564cervical adenitis
53802Cervical adenitis

isolation source categories

Cat1Cat2Cat3
#Infection#Inflammation
#Host Body-Site#Oral cavity and airways#Throat
#Host Body-Site#Organ#Lymph node

Safety information

risk assessment

@refpathogenicity humanpathogenicity animalbiosafety levelbiosafety level comment
11564yesyes2Risk group (German classification)
200102German classification

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Mycobacterium szulgai strain ATCC 35799 16S ribosomal RNA gene, partial sequenceKC9512781486ena1787
20218M.szulgai 16S ribosomal RNA geneM59274146ena1787
20218M.szulgai 16S ribosomal rRNAM616651379ena1787
20218Mycobacterium szulgai 16S rRNA geneX529261462ena1787
20218M.szulgai internal transcribed spacer between 16S and 23S rRNA, genomic sequenceX99220275ena1787
20218Mycobacterium szulgai strain CIP 104532 16S ribosomal RNA gene, partial sequenceAF547969554ena1787
20218Mycobacterium szulgai DNA for 16S rRNA, internal transcribed spacer, 23S rRNAAB026704433ena1787

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Mycobacterium szulgai DSM 44166GCA_002116635contigncbi1787
66792Mycobacterium szulgai strain 1146974.21787.4wgspatric1787
66792Mycobacterium szulgai strain ACS11601787.3wgspatric1787
66792Mycobacterium szulgai strain DSM 441661787.5wgspatric1787

External links

@ref: 11564

culture collection no.: DSM 44166, ATCC 35799, JCM 6383, NCTC 10831, PCM 1876, TMC 1328, CCUG 37675

straininfo link

@refpassport
20218http://www.straininfo.net/strains/169283
20218http://www.straininfo.net/strains/111290
20218http://www.straininfo.net/strains/139735
20218http://www.straininfo.net/strains/153085
20218http://www.straininfo.net/strains/169284
20218http://www.straininfo.net/strains/111291

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
11564Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 44166)https://www.dsmz.de/collection/catalogue/details/culture/DSM-44166
20010Wink, J.http://www.dsmz.de/microorganisms/wink_pdf/DSM44166.pdfCompendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig
20215D.Gleim, M.Kracht, N.Weiss et. al.http://www.dsmz.de/bacterial-diversity/prokaryotic-nomenclature-up-to-date.htmlProkaryotic Nomenclature Up-to-date - compilation of all names of Bacteria and Archaea, validly published according to the Bacteriological Code since 1. Jan. 1980, and validly published nomenclatural changes since
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
36461Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/16451
53802Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 37675)https://www.ccug.se/strain?id=37675
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)