Strain identifier

BacDive ID: 8362

Type strain: Yes

Species: Mycobacterium parafortuitum

Strain Designation: 16002, P. Hauduroy 2490, 311

Strain history: CIP <- 2000, JCM <- M. Tsukamura: strain 16002

NCBI tax ID(s): 39692 (species)

« back to strain detail view
For citation purpose refer to the digital object identifier (doi) of the current version.
Archive
version 8.1 (current version):
version 8:
version 7.1:
version 7:
version 6:
version 5:
version 4.1:
version 4:
version 3:
version 2.1:
version 2:
version 1:
version 8.1 (current version)

General

@ref: 11116

BacDive-ID: 8362

DSM-Number: 43528

keywords: genome sequence, 16S sequence, Bacteria, obligate aerobe, mesophilic

description: Mycobacterium parafortuitum 16002 is an obligate aerobe, mesophilic bacterium that was isolated from soil.

NCBI tax id

  • NCBI tax id: 39692
  • Matching level: species

strain history

@refhistory
11116<- I. Tarnok, SN 1603 <- ATCC <- M. Tsukamura, 311
67770M. Tsukamura 16002.
121556CIP <- 2000, JCM <- M. Tsukamura: strain 16002

doi: 10.13145/bacdive8362.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Mycobacteriales
  • family: Mycobacteriaceae
  • genus: Mycobacterium
  • species: Mycobacterium parafortuitum
  • full scientific name: Mycobacterium parafortuitum Tsukamura 1966 (Approved Lists 1980)

  • synonyms

    • @ref: 20215
    • synonym: Mycolicibacterium parafortuitum

@ref: 11116

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Mycobacteriaceae

genus: Mycobacterium

species: Mycobacterium parafortuitum

full scientific name: Mycobacterium parafortuitum Tsukamura 1966

strain designation: 16002, P. Hauduroy 2490, 311

type strain: yes

Morphology

cell morphology

@refmotilityconfidencegram staincell shape
69480no93.957
69480100positive
121556norod-shaped

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
11116GYM+S MEDIUM (DSMZ Medium 214)yeshttps://mediadive.dsmz.de/medium/214Name: GYM+S MEDIUM (DSMZ Medium 214) Composition: Starch 20.0 g/l Agar 12.0 g/l Malt extract 10.0 g/l Yeast extract 4.0 g/l Glucose 4.0 g/l CaCO3 2.0 g/l Distilled water
11116BHI MEDIUM WITH ADDITIONAL GLUCOSE (DSMZ Medium 215b)yeshttps://mediadive.dsmz.de/medium/215bName: BHI MEDIUM WITH ADDITIONAL GLUCOSE (DSMZ Medium 215b) Composition: Brain heart infusion 37.0 g/l Glucose 5.0 g/l Distilled water
11116LÖWENSTEIN-JENSEN MEDIUM (DSMZ Medium 354)yeshttps://mediadive.dsmz.de/medium/354Name: LÖWENSTEIN-JENSEN MEDIUM (DSMZ Medium 354) Composition: Potato flour 18.6104 g/l L-Asparagin 2.23325 g/l KH2PO4 1.55087 g/l Mg-citrate 0.372208 g/l Malachite green 0.248139 g/l MgSO4 0.148883 g/l Glycerol Fresh egg mixture Distilled water
38649MEDIUM 112 - for JanibacteryesDistilled water make up to (1000.000 ml);Magnesium sulphate heptahydrate (1.000 g);Agar (20.000 g);Yeast extract (5.000 g);Peptone (10.000 g);Tween 80 (50.000 mg);Glycerol (2.000 g);Malt extract (5.000 g);Beef extract (2.000 g);Casamino acids (5.000 g);Ca
121556CIP Medium 112yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=112

culture temp

@refgrowthtypetemperaturerange
11116positivegrowth37mesophilic
38649positivegrowth37mesophilic
67770positivegrowth37mesophilic
121556positivegrowth22-37
121556nogrowth10psychrophilic
121556nogrowth45thermophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 121556
  • oxygen tolerance: obligate aerobe

spore formation

@refspore formationconfidence
69481no96
69480no99.825

observation

  • @ref: 67770
  • observation: quinones: MK-9(H2)

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6836925115malate+assimilation
6836927689decanoate-assimilation
6836924265gluconate+assimilation
6836917306maltose+assimilation
6836959640N-acetylglucosamine-assimilation
6836916899D-mannitol+assimilation
6836916024D-mannose-assimilation
6836930849L-arabinose+assimilation
6836917634D-glucose+assimilation
683695291gelatin+hydrolysis
6836916199urea+hydrolysis
6836929016arginine-hydrolysis
6836917634D-glucose-fermentation
6836927897tryptophan-energy source
6836917632nitrate+reduction
121556606565hippurate-hydrolysis
12155617632nitrate-reduction
12155616301nitrite-reduction
12155617632nitrate-respiration

antibiotic resistance

  • @ref: 121556
  • metabolite: 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)
  • is antibiotic: yes
  • is sensitive: yes
  • is resistant: no

metabolite production

@refChebi-IDmetaboliteproduction
6836935581indoleno
12155635581indoleno

metabolite tests

  • @ref: 68369
  • Chebi-ID: 35581
  • metabolite: indole
  • indole test: -

enzymes

@refvalueactivityec
68369gelatinase+
68369urease+3.5.1.5
68369arginine dihydrolase-3.5.3.6
121556oxidase-
121556beta-galactosidase-3.2.1.23
121556gelatinase-
121556amylase-
121556DNase-
121556caseinase-3.4.21.50
121556catalase+1.11.1.6
121556tween esterase-
121556gamma-glutamyltransferase-2.3.2.2
121556lecithinase-
121556lipase-
121556protease-
121556urease+3.5.1.5
68382alkaline phosphatase+3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)+
68382leucine arylamidase+3.4.11.1
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase+3.1.3.2
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase-3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382beta-glucosidase-3.2.1.21
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

fatty acid profile

  • fatty acids

    @reffatty acidpercentageECL
    65606C14:03.814
    65606C16:014.516
    65606C18:00.518
    65606C14:0 N alcohol22.213.524
    65606C16:1 ω7c/C15:0 ISO 2OH7.215.85
    65606C16:1 ω9c1.115.774
    65606C17:1 ω7c16.716.818
    65606C18:1 ω9c10.917.769
    65606C18:2 ω6,9c/C18:0 ANTE1.517.724
    65606C20:0 alcohol (C20:0 2OH alc)15.918.835
    65606TBSA 10Me18:03.118.392
    65606Unidentified0.911.5
    65606Unidentified0.514.817
    65606Unidentified1.318.515
  • type of FA analysis: whole cell analysis
  • method/protocol: CCUG

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
11116-+++-+-+/---++/----+/-----
121556-++++++++-++---+----

API 20NE

@refNO3TRPGLU_ FermADH ArgUREESCGELPNPGGLU_ AssimARAMNEMANNAGMALGNTCAPADIMLTCITPAC
11116+---++/-++/-++-+-++-+/-+--

Isolation, sampling and environmental information

isolation

@refsample type
11116soil
67770Soil
121556Environment, Soil

isolation source categories

  • Cat1: #Environmental
  • Cat2: #Terrestrial
  • Cat3: #Soil

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
111161Risk group (German classification)
1215561Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Mycobacterium parafortuitum strain CIP 106802 16S ribosomal RNA gene, partial sequenceAF547955540ena39692
20218M.parafortuitum 16S rRNA geneX931831460ena39692

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Mycolicibacterium parafortuitum JCM 6367GCA_010725485completencbi39692
66792Mycobacterium parafortuitum strain CCUG 2099939692.5wgspatric39692
66792Mycobacterium parafortuitum strain CCUG 2099939692.4wgspatric39692
66792Mycolicibacterium parafortuitum strain JCM 636739692.16completepatric39692
66792Mycolicibacterium parafortuitum CCUG 209992865573456draftimg39692
67770Mycolicibacterium parafortuitum CCUG 20999GCA_002086815contigncbi39692

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno96no
motileno90.006no
flagellatedno97.932no
gram-positiveyes88.657no
anaerobicno99.284no
aerobicyes89.71no
halophileno89.519no
spore-formingno85.676no
thermophileno99.485no
glucose-utilyes88.306no
glucose-fermentno91.424yes

External links

@ref: 11116

culture collection no.: DSM 43528, ATCC 19686, CCUG 20999, CIP 106802, JCM 6367, NCTC 10411, NRRL B-4035, SN 1603

straininfo link

  • @ref: 77762
  • straininfo: 92783

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
11116Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 43528)https://www.dsmz.de/collection/catalogue/details/culture/DSM-43528
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
38649Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/18972
65606Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 20999)https://www.ccug.se/strain?id=20999
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68369Automatically annotated from API 20NE
68382Automatically annotated from API zym
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
77762Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID92783.1StrainInfo: A central database for resolving microbial strain identifiers
121556Curators of the CIPCollection of Institut Pasteur (CIP 106802)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20106802