Strain identifier

BacDive ID: 8334

Type strain: No

Species: Mycobacterium austroafricanum

Strain Designation: JOB-5

Strain history: CIP <- 1998, ATCC <- J.W. Foster <- J.J. Perry <- J. Ooyama

NCBI tax ID(s): 39687 (species)

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General

@ref: 5271

BacDive-ID: 8334

DSM-Number: 14262

keywords: 16S sequence, Bacteria, obligate aerobe, rod-shaped

description: Mycobacterium austroafricanum JOB-5 is an obligate aerobe, rod-shaped bacterium that was isolated from soil.

NCBI tax id

  • NCBI tax id: 39687
  • Matching level: species

strain history

@refhistory
5271<- ATCC <- J. J. Perry <- J. W. Foster <- J. Ooyama, Univ. of Texas
116651CIP <- 1998, ATCC <- J.W. Foster <- J.J. Perry <- J. Ooyama

doi: 10.13145/bacdive8334.20240510.9

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Mycobacteriales
  • family: Mycobacteriaceae
  • genus: Mycobacterium
  • species: Mycobacterium austroafricanum
  • full scientific name: Mycobacterium austroafricanum Tsukamura et al. 1983

  • synonyms

    @refsynonym
    20215Mycobacterium vanbaalenii
    20215Mycolicibacterium austroafricanum
    20215Mycolicibacterium vanbaalenii

@ref: 5271

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Mycobacteriaceae

genus: Mycobacterium

species: Mycobacterium austroafricanum

full scientific name: Mycobacterium sp.

strain designation: JOB-5

type strain: no

Morphology

cell morphology

  • @ref: 116651
  • cell shape: rod-shaped
  • motility: no

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
5271MIDDLEBROOK MEDIUM (DSMZ Medium 645)yeshttps://mediadive.dsmz.de/medium/645Name: MIDDLEBROOK MEDIUM (DSMZ Medium 645) Composition: Bacto Middlebrook 7H10 agar 20.9945 g/l Glycerol Distilled water
5271MINERAL MEDIUM (NAGEL AND ANDREESEN) (DSMZ Medium 461)yeshttps://mediadive.dsmz.de/medium/461Name: MINERAL MEDIUM (NAGEL AND ANDREESEN) (DSMZ Medium 461; with strain-specific modifications) Composition: Na2HPO4 x 2 H2O 1.44135 g/l Hexadecane 1.0 g/l MgSO4 x 7 H2O 0.497018 g/l NH4Cl 0.298211 g/l KH2PO4 0.248509 g/l NaCl 0.0497018 g/l MnSO4 0.00994036 g/l CaCl2 0.00994036 g/l HCl 0.00248509 g/l FeCl2 x 4 H2O 0.00149105 g/l p-Aminobenzoic acid 0.000248509 g/l Thiamine-HCl x 2 H2O 0.000248509 g/l alpha-lipoic acid 0.000248509 g/l Vitamin B12 0.000248509 g/l Pantothenic acid 0.000248509 g/l Riboflavin 0.000248509 g/l CoCl2 x 6 H2O 0.000188867 g/l Nicotine amide 0.000124254 g/l Nicotinic acid 0.000124254 g/l Folic acid 9.94036e-05 g/l Biotin 9.94036e-05 g/l MnCl2 x 4 H2O 9.94036e-05 g/l ZnCl2 6.95825e-05 g/l Pyridoxamine hydrochloride 4.97018e-05 g/l Na2MoO4 x 2 H2O 3.57853e-05 g/l NiCl2 x 6 H2O 2.38569e-05 g/l H3BO3 5.96421e-06 g/l CuCl2 x 2 H2O 1.98807e-06 g/l Distilled water
36253MEDIUM 3 - Columbia agaryesColumbia agar (39.000 g);distilled water (1000.000 ml)
116651CIP Medium 3yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=3
116651CIP Medium 55yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=55

culture temp

@refgrowthtypetemperature
5271positivegrowth28
36253positivegrowth30
116651positivegrowth22-37
116651nogrowth10
116651nogrowth45

Physiology and metabolism

oxygen tolerance

  • @ref: 116651
  • oxygen tolerance: obligate aerobe

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
116651606565hippurate-hydrolysis
11665117632nitrate+reduction
11665116301nitrite-reduction
11665117632nitrate-respiration

metabolite production

  • @ref: 116651
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

enzymes

@refvalueactivityec
116651oxidase-
116651beta-galactosidase+3.2.1.23
116651gelatinase-
116651amylase-
116651DNase-
116651caseinase-3.4.21.50
116651catalase+1.11.1.6
116651tween esterase+
116651gamma-glutamyltransferase-2.3.2.2
116651lecithinase-
116651lipase-
116651protease-
116651urease+3.5.1.5
68382alkaline phosphatase-3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)+
68382lipase (C 14)-
68382leucine arylamidase+3.4.11.1
68382valine arylamidase+
68382cystine arylamidase+3.4.11.3
68382trypsin+3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase-3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase+3.2.1.20
68382beta-glucosidase-3.2.1.21
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
116651--++-++++-++---+----

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
116651++-----+------------++----------++---------------+----------+------------------++---+---+-------+--

Isolation, sampling and environmental information

isolation

@refsample typecountryorigin.countrycontinentgeographic location
5271soilUSAUSANorth America
116651Environment, SoilUnited KingdomGBREuropeLeicester

isolation source categories

  • Cat1: #Environmental
  • Cat2: #Terrestrial
  • Cat3: #Soil

Safety information

risk assessment

  • @ref: 116651
  • biosafety level: 1
  • biosafety level comment: Risk group (French classification)

Sequence information

16S sequences

  • @ref: 20218
  • description: Mycobacterium austroafricanum strain JOB5 16S ribosomal RNA gene, partial sequence
  • accession: HQ331480
  • length: 1330
  • database: nuccore
  • NCBI tax ID: 39687

External links

@ref: 5271

culture collection no.: DSM 14262, ATCC 29678, CIP 105723

straininfo link

  • @ref: 77736
  • straininfo: 39652

literature

  • topic: Metabolism
  • Pubmed-ID: 17347817
  • title: n-Alkane assimilation and tert-butyl alcohol (TBA) oxidation capacity in Mycobacterium austroafricanum strains.
  • authors: Lopes Ferreira N, Mathis H, Labbe D, Monot F, Greer CW, Fayolle-Guichard F
  • journal: Appl Microbiol Biotechnol
  • DOI: 10.1007/s00253-007-0892-1
  • year: 2007
  • mesh: Alkanes/*metabolism, Amino Acid Sequence, Bacterial Proteins/genetics/metabolism, Biodegradation, Environmental, DNA, Bacterial/genetics, DNA, Ribosomal/genetics, Gene Expression, Genome, Bacterial, Mixed Function Oxygenases/genetics/*metabolism, Molecular Sequence Data, Mycobacterium/classification/genetics/growth & development/*metabolism, Oxidation-Reduction, Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Alignment, tert-Butyl Alcohol/*metabolism
  • topic2: Genetics

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
5271Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 14262)https://www.dsmz.de/collection/catalogue/details/culture/DSM-14262
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
36253Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/17774
68382Automatically annotated from API zym
77736Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID39652.1StrainInfo: A central database for resolving microbial strain identifiers
116651Curators of the CIPCollection of Institut Pasteur (CIP 105723)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20105723