Strain identifier

BacDive ID: 8285

Type strain: Yes

Species: Mycobacterium phlei

Strain Designation: Timoteo, 14 130 0001

Strain history: CIP <- 1998, V. Vincent, Inst. Pasteur, Paris, France: strain 14 130 0001 <- ATCC <- G. Penso: strain Timoteo

NCBI tax ID(s): 1226750 (strain), 1771 (species)

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General

@ref: 10895

BacDive-ID: 8285

DSM-Number: 43239

keywords: genome sequence, 16S sequence, Bacteria, obligate aerobe, mesophilic

description: Mycobacterium phlei Timoteo is an obligate aerobe, mesophilic bacterium of the family Mycobacteriaceae.

NCBI tax id

NCBI tax idMatching level
1226750strain
1771species

strain history

@refhistory
10895<- NCTC <- G. Penso, Timoteo
67770NCTC 8151 <-- G. Penso strain Timoteo.
121799CIP <- 1998, V. Vincent, Inst. Pasteur, Paris, France: strain 14 130 0001 <- ATCC <- G. Penso: strain Timoteo

doi: 10.13145/bacdive8285.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Mycobacteriales
  • family: Mycobacteriaceae
  • genus: Mycobacterium
  • species: Mycobacterium phlei
  • full scientific name: Mycobacterium phlei Lehmann and Neumann 1899 (Approved Lists 1980)

  • synonyms

    • @ref: 20215
    • synonym: Mycolicibacterium phlei

@ref: 10895

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Mycobacteriaceae

genus: Mycobacterium

species: Mycobacterium phlei

full scientific name: Mycobacterium phlei Lehmann and Neumann 1899 emend. Nouioui et al. 2018

strain designation: Timoteo, 14 130 0001

type strain: yes

Morphology

cell morphology

@refmotilityconfidencegram staincell shape
69480no94.036
69480100positive
121799norod-shaped

colony morphology

  • @ref: 47249
  • incubation period: 2 days

multimedia

  • @ref: 10895
  • multimedia content: https://www.dsmz.de/microorganisms/photos/DSM_43239.jpg
  • caption: Medium 250 37°C
  • intellectual property rights: © Leibniz-Institut DSMZ

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
10895LÖWENSTEIN-JENSEN MEDIUM (DSMZ Medium 354)yeshttps://mediadive.dsmz.de/medium/354Name: LÖWENSTEIN-JENSEN MEDIUM (DSMZ Medium 354) Composition: Potato flour 18.6104 g/l L-Asparagin 2.23325 g/l KH2PO4 1.55087 g/l Mg-citrate 0.372208 g/l Malachite green 0.248139 g/l MgSO4 0.148883 g/l Glycerol Fresh egg mixture Distilled water
10895PEPTONE MEAT EXTRACT GLYCEROL AGAR (DSMZ Medium 250)yeshttps://mediadive.dsmz.de/medium/250Name: PEPTONE MEAT EXTRACT GLYCEROL AGAR (DSMZ Medium 250) Composition: Agar 20.0 g/l Proteose peptone no. 3 5.0 g/l Meat extract 3.0 g/l Glycerol Distilled water
36395MEDIUM 55 - for Mycobacteriumyes
121799CIP Medium 55yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=55

culture temp

@refgrowthtypetemperaturerange
10895positivegrowth37mesophilic
36395positivegrowth37mesophilic
47249positivegrowth30-37mesophilic
67770positivegrowth37mesophilic
121799positivegrowth25-45
121799nogrowth10psychrophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 121799
  • oxygen tolerance: obligate aerobe

spore formation

@refspore formationconfidence
69481no100
69480no98.494

observation

  • @ref: 67770
  • observation: quinones: MK-9(H2)

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6836925115malate+assimilation
6836927689decanoate-assimilation
6836924265gluconate+assimilation
6836917306maltose-assimilation
6836916899D-mannitol+assimilation
6836930849L-arabinose+assimilation
6836917634D-glucose+assimilation
683695291gelatin-hydrolysis
683694853esculin-hydrolysis
6836916199urea+hydrolysis
6836929016arginine-hydrolysis
6836917634D-glucose-fermentation
6836927897tryptophan-energy source
6836917632nitrate+reduction
121799606565hippurate-hydrolysis
12179917632nitrate+reduction
12179916301nitrite-reduction
12179917632nitrate-respiration
6837917632nitrate+reduction
683794853esculin+hydrolysis
6837916199urea+hydrolysis
683795291gelatin-hydrolysis
6837916988D-ribose-fermentation
6837965327D-xylose-fermentation
6837916899D-mannitol-fermentation
6837917306maltose-fermentation
6837917716lactose-fermentation
6837917992sucrose-fermentation
6837928087glycogen-fermentation

metabolite production

@refChebi-IDmetaboliteproduction
6836935581indoleno
12179935581indoleno

metabolite tests

  • @ref: 68369
  • Chebi-ID: 35581
  • metabolite: indole
  • indole test: -

enzymes

@refvalueactivityec
68379catalase+1.11.1.6
68379gelatinase-
68379urease+3.5.1.5
68379beta-glucosidase+3.2.1.21
68379N-acetyl-beta-glucosaminidase-3.2.1.52
68379alpha-glucosidase-3.2.1.20
68379beta-galactosidase-3.2.1.23
68379beta-glucuronidase-3.2.1.31
68379alkaline phosphatase-3.1.3.1
68379pyrrolidonyl arylamidase+3.4.19.3
68379pyrazinamidase+3.5.1.B15
68369gelatinase-
68369beta-glucosidase-3.2.1.21
68369urease+3.5.1.5
68369arginine dihydrolase-3.5.3.6
121799oxidase-
121799beta-galactosidase-3.2.1.23
121799gelatinase-
121799amylase-
121799DNase-
121799caseinase-3.4.21.50
121799catalase+1.11.1.6
121799tween esterase-
121799gamma-glutamyltransferase-2.3.2.2
121799lecithinase+
121799lipase-
121799urease+3.5.1.5
68382alkaline phosphatase-3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)+
68382lipase (C 14)-
68382leucine arylamidase+3.4.11.1
68382cystine arylamidase-3.4.11.3
68382trypsin-3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase-3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase-3.2.1.20
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

fatty acid profile

  • fatty acids

    @reffatty acidpercentageECL
    47249C14:02.514
    47249C15:00.815
    47249C16:028.916
    47249C17:00.717
    47249C16:0 10-methyl0.916.433
    47249C16:1 ω7c1.715.819
    47249C16:1 ω7c/C15:0 ISO 2OH7.515.85
    47249C16:1 ω9c1.315.774
    47249C17:0 10-methyl1.217.41
    47249C17:1 ω7c1216.818
    47249C18:1 ω7c /12t/9t1.317.824
    47249C18:1 ω9c11.617.769
    47249C18:2 ω6,9c/C18:0 ANTE0.917.724
    47249C20:0 alcohol (C20:0 2OH alc)15.618.835
    47249TBSA 10Me18:012.318.392
    47249Unidentified0.919.235
  • type of FA analysis: whole cell analysis
  • method/protocol: CCUG

API coryne

@refNITPYZPYRAPALbeta GURbeta GALalpha GLUbeta NAGESCUREGELControlRIBXYLMANMALLACSACGLYGCAT
47249+++-----++---------+

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
10895--++-++/----++----+/----
121799--++-++---++--------

API 20NE

@refNO3TRPGLU_ FermADH ArgUREESCGELPNPGGLU_ AssimARAMNEMANNAGMALGNTCAPADIMLTCITPAC
10895+---+--++++/-++/--+-+/-++/--

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
121799+++++---------------++----+++--+-+--++--+-------++------+---+-----------+--+--+++-------+--+-+--+-+

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
108951Risk group (German classification)
1217991Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218M.phlei 16S ribosomal RNAM295661357ena1771
10895Mycobacterium phlei 16S ribosomal RNA gene, partial sequenceAF4806031461ena1226750

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Mycolicibacterium phlei CCUG 21000GCA_001583415completencbi1771
66792Mycobacterium phlei DSM 43239 = CCUG 210001226750.3wgspatric1226750
66792Mycobacterium phlei strain CCUG 210001771.11completepatric1771
66792Mycolicibacterium phlei strain NCTC81511771.16completepatric1771
66792Mycolicibacterium phlei DSM 432392728369596draftimg1226750
66792Mycolicibacterium phlei CCUG210002684623205completeimg1771
67770Mycolicibacterium phlei DSM 43239 = CCUG 21000GCA_001582015contigncbi1226750
67770Mycolicibacterium phlei DSM 43239 = CCUG 21000GCA_009192875contigncbi1226750
67770Mycolicibacterium phlei NCTC8151GCA_900637085completencbi1771

GC content

  • @ref: 67770
  • GC-content: 69.4
  • method: genome sequence analysis

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno100no
flagellatedno98.231no
gram-positiveyes91.103no
anaerobicno99.489no
aerobicyes85.102no
halophileno89.386no
spore-formingno78.51no
glucose-utilyes86.928no
motileno92.881no
thermophileno99.067yes
glucose-fermentno90.53yes

External links

@ref: 10895

culture collection no.: DSM 43239, ATCC 11758, ATCC 19249, NCTC 8151, CCUG 21000, TMC 1548, JCM 5865, BCRC 10707, CECT 3009, CIP 105389, JCM 6385, KCTC 9087, NRIC 1711, NRRL B-14615, PCM 654, RIMD 1326001, VKM Ac-1291

straininfo link

  • @ref: 77687
  • straininfo: 35419

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Metabolism1319278Correlation between calmodulin-like protein, phospholipids, and growth in glucose-grown Mycobacterium phlei.Reddy PH, Burra SS, Murthy PSCan J Microbiol10.1139/m92-0571992Bacterial Proteins/*metabolism, Calcium/metabolism, Calmodulin/*metabolism, Cyclic AMP/metabolism, Glucose/metabolism, Mycobacterium phlei/drug effects/growth & development/*metabolism, Phospholipids/*metabolism, Trifluoperazine/pharmacologyPathogenicity
Genetics8794763Chemistry of the lyxose-containing mycobacteriophage receptors of Mycobacterium phlei/Mycobacterium smegmatis.Khoo KH, Suzuki R, Dell A, Morris HR, McNeil MR, Brennan PJ, Besra GSBiochemistry10.1021/bi961055+1996Carbohydrate Conformation, Carbohydrate Sequence, Chromatography, Gas, Galactans/analysis, Glycolipids/*chemistry/isolation & purification, Lipopolysaccharides/analysis, Magnetic Resonance Spectroscopy, Mass Spectrometry, Molecular Sequence Data, Mycobacteriophages/*metabolism, Mycobacterium/*chemistry/virology, Oligosaccharides/analysis/chemistry, Pentoses/*analysis, Receptors, Virus/*chemistryEnzymology
Pathogenicity25697605Antimicrobial activity of natural products from the flora of Northern Ontario, Canada.Vandal J, Abou-Zaid MM, Ferroni G, Leduc LGPharm Biol10.3109/13880209.2014.9428672015Anti-Bacterial Agents/pharmacology, Anti-Infective Agents/*pharmacology, Antifungal Agents/pharmacology, Bacteria/drug effects, Biological Products/*pharmacology, Chromatography, High Pressure Liquid, Fungi/drug effects, Indicators and Reagents, Microbial Sensitivity Tests, Ontario, Oxazines, Plant Extracts/pharmacology, Plants/*chemistry, Xanthenes
Metabolism26941228The Mycobacterium phlei Genome: Expectations and Surprises.Das S, Pettersson BM, Behra PR, Ramesh M, Dasgupta S, Bhattacharya A, Kirsebom LAGenome Biol Evol10.1093/gbe/evw0492016Animals, CRISPR-Cas Systems, Gene Transfer, Horizontal, *Genome, Bacterial, Glycerol/metabolism, Mycobacterium phlei/*genetics/growth & development/metabolism, Phylogeny, Polyamines/metabolismGenetics

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
10895Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 43239)https://www.dsmz.de/collection/catalogue/details/culture/DSM-43239
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
36395Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/17403
47249Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 21000)https://www.ccug.se/strain?id=21000
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68369Automatically annotated from API 20NE
68379Automatically annotated from API Coryne
68382Automatically annotated from API zym
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
77687Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID35419.1StrainInfo: A central database for resolving microbial strain identifiers
121799Curators of the CIPCollection of Institut Pasteur (CIP 105389)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20105389