Strain identifier

BacDive ID: 8273

Type strain: Yes

Species: Mycobacterium marinum

Strain Designation: 140120001

Strain history: CIP <- 1995, V. Vincent, Inst. Pasteur, Paris, France: strain 14 012 0001 <- ATCC <- R.E. Gordon <- (J. Aronson)

NCBI tax ID(s): 1781 (species)

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General

@ref: 11690

BacDive-ID: 8273

DSM-Number: 44344

keywords: genome sequence, 16S sequence, Bacteria, obligate aerobe, mesophilic

description: Mycobacterium marinum 140120001 is an obligate aerobe, mesophilic bacterium that was isolated from dead fish in aquarium.

NCBI tax id

  • NCBI tax id: 1781
  • Matching level: species

strain history

@refhistory
11690<- ATCC; ATCC 927 <- R. E. Gordon; TMC 1218 <- J. D. Aronson;
67770NCTC 2275 <-- J. D. Aronson.
120812CIP <- 1995, V. Vincent, Inst. Pasteur, Paris, France: strain 14 012 0001 <- ATCC <- R.E. Gordon <- (J. Aronson)

doi: 10.13145/bacdive8273.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/actinomycetota
  • domain: Bacteria
  • phylum: Actinomycetota
  • class: Actinomycetes
  • order: Mycobacteriales
  • family: Mycobacteriaceae
  • genus: Mycobacterium
  • species: Mycobacterium marinum
  • full scientific name: Mycobacterium marinum Aronson 1926 (Approved Lists 1980)

@ref: 11690

domain: Bacteria

phylum: Actinobacteria

class: Actinobacteria

order: Actinomycetales

family: Mycobacteriaceae

genus: Mycobacterium

species: Mycobacterium marinum

full scientific name: Mycobacterium marinum Aronson 1926

strain designation: 140120001

type strain: yes

Morphology

cell morphology

@refmotilityconfidencegram staincell shape
69480no96.458
69480100positive
120812nopositiverod-shaped

colony morphology

@refcolony colorincubation periodmedium used
20047Golden yellow (1004)10-14 days5006
20047Golden yellow (1004)10-14 daysBHI
20047Golden yellow (1004)10-14 daysBUG
20047Golden yellow (1004)10-14 daysMB7H9

multimedia

@refmultimedia contentintellectual property rightscaption
11690https://www.dsmz.de/microorganisms/photos/DSM_44344-1.jpg© Leibniz-Institut DSMZ
11690https://www.dsmz.de/microorganisms/photos/DSM_44344.jpg© Leibniz-Institut DSMZMedium 645 37°C

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
11690MIDDLEBROOK MEDIUM (DSMZ Medium 645)yeshttps://mediadive.dsmz.de/medium/645Name: MIDDLEBROOK MEDIUM (DSMZ Medium 645) Composition: Bacto Middlebrook 7H10 agar 20.9945 g/l Glycerol Distilled water
20047BUGyes
20047BHIyes
200475006yesMedium: 5006 Name: Composition (g/l) Sucrose 3,0 Dextrin 15,0 Meat extract 1,0 Yeast extract 2,0 Tryptone soy broth (Oxoid) 5,0 NaCl 0,5 K2HPO4 0,5 MgSO4 x 7 H2O 0,5 FeSO4 x 7 H2O 0,01 Agar 20,0 Preparation: Sterilisation: 20 minutes at 121°C pH before sterilisation: 7,3 Usage: Maintenance Organisms: All Actinomycetes
20047MB7H9yesName: Middlebrook 7H9 (MB7H9) Broth Base Composition: Middelbrook 7H9 Broth Base 4.7 g/l Glycerol 2.0 g/l Casaminoacids 11.0 g/l Yeast extract 5.5 g/l Tween80 (20%) 2.5 g/l OADC-Enrichment 100 ml Agar 15.0 g/l Preparation: Dissolve in Milli-Q-H2O. Before Use add 10% OADC stock solution Sterilisation: 20 minutes at 121°C pH before sterilisation: Usage: Maintenance and revitalization Organisms: Mycobacteria OADC stock solution old broth-name: OADC-Stock Composition: Bovine Serum Albumin, fraction V 5 % 1% Oleic Acid in 0.2N NaOH 5ml Glucose 2 % NaCl 0.85 % Comments: Melt 1% Oleic Acid in 65°C water bath. Dissolve all ingredients in 700ml of ddH2O. Adjust volume to 1L. Filter sterilize and store at 4°C. -To make 1% Oleic Acid in 0.2N NaOH: Dissolve 5g oleic acid (ampule) in 500ml 0.2N NaOH (300ml ddH2O + 20ml NaOH + adjust volume to 500ml). Heat solution to 55°C for oleic acid to melt. Store at -20°C in 50ml aliquots. There is no need to sterilize this. Sterilisation: 20 minutes Temperature:121°C PH before: PH after: Biological details Culture type: suitable for: Mycobacteria
36349MEDIUM 55 - for Mycobacteriumyes
120812CIP Medium 55yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=55

culture temp

@refgrowthtypetemperaturerange
20047positiveoptimum37mesophilic
11690positivegrowth28mesophilic
36349positivegrowth30mesophilic
67770positivegrowth25mesophilic
120812positivegrowth30mesophilic
120812nogrowth5psychrophilic
120812nogrowth10psychrophilic
120812nogrowth37mesophilic
120812nogrowth41thermophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 120812
  • oxygen tolerance: obligate aerobe

spore formation

  • @ref: 69480
  • spore formation: no
  • confidence: 99.823

halophily

@refsaltgrowthtested relationconcentration
120812NaClpositivegrowth0-6 %
120812NaClnogrowth8 %
120812NaClnogrowth10 %

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
1208124853esculin-hydrolysis
120812606565hippurate+hydrolysis
12081217632nitrate-reduction
12081216301nitrite-reduction
12081217632nitrate-respiration
6837917632nitrate-reduction
683794853esculin-hydrolysis
6837916199urea-hydrolysis
683795291gelatin-hydrolysis
6837917634D-glucose-fermentation
6837916988D-ribose-fermentation
6837965327D-xylose-fermentation
6837916899D-mannitol-fermentation
6837917306maltose-fermentation
6837917716lactose-fermentation
6837917992sucrose-fermentation
6837928087glycogen-fermentation

metabolite production

  • @ref: 120812
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

metabolite tests

  • @ref: 120812
  • Chebi-ID: 15688
  • metabolite: acetoin
  • voges-proskauer-test: -

enzymes

@refvalueactivityec
68379gelatinase-
68379urease-3.5.1.5
68379beta-glucosidase-3.2.1.21
68379N-acetyl-beta-glucosaminidase-3.2.1.52
68379alpha-glucosidase+3.2.1.20
68379beta-galactosidase-3.2.1.23
68379beta-glucuronidase-3.2.1.31
68379alkaline phosphatase+3.1.3.1
68379pyrrolidonyl arylamidase+3.4.19.3
68379pyrazinamidase-3.5.1.B15
120812oxidase-
120812beta-galactosidase-3.2.1.23
120812alcohol dehydrogenase-1.1.1.1
120812gelatinase-
120812amylase-
120812DNase-
120812caseinase-3.4.21.50
120812catalase+1.11.1.6
120812tween esterase+
120812lecithinase+
120812lipase-
120812lysine decarboxylase-4.1.1.18
120812ornithine decarboxylase-4.1.1.17
120812urease+3.5.1.5
68382alkaline phosphatase+3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)+
68382leucine arylamidase+3.4.11.1
68382trypsin-3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase-3.2.1.22
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase+3.2.1.51

API coryne

@refNITPYZPYRAPALbeta GURbeta GALalpha GLUbeta NAGESCUREGELGLURIBXYLMANMALLACSACGLYG
20047--++--+------------

API zym

@refAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- FucosidaseControl
20047+++++++--++-++++--+
120812+++-+----++-------+-

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
120812---------------------------------------------------------------------------+---+----------+--+-----

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentlatitudelongitude
11690dead fish in aquariumPhiladelphiaUSAUSANorth America39.95-75.1667
67770Fish tuberculosis lesionPhiladelphia, PAUSAUSANorth America
120812Animal, Fish (salt water)Philadelphia, PennsylvaniaUnited States of AmericaUSANorth America

isolation source categories

  • Cat1: #Host
  • Cat2: #Fishes

taxonmaps

  • @ref: 69479
  • File name: preview.99_691.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_10;96_37;97_39;98_43;99_691&stattab=map
  • Last taxonomy: Mycobacterium
  • 16S sequence: AF456240
  • Sequence Identity:
  • Total samples: 2597
  • soil counts: 1494
  • aquatic counts: 660
  • animal counts: 250
  • plant counts: 193

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
116902Risk group (German classification)
200472German classification
1208122Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Mycobacterium marinum gene for 16S ribosomal RNA, partial sequence, strain: ATCC 927AB5487171475ena1781
20218Mycobacterium marinum DNA, 16S-23S ribosomal RNA intergenic spacer, partial sequence, strain: ATCC 927AB548718272ena1781
20218Mycobacterium marinum gene for 16S ribosomal RNA, partial sequenceAB6361341483ena1781
20218Mycobacterium marinum strain ATCC 927 16S ribosomal RNA gene, partial sequenceAF4562401522ena1781
20218Mycobacterium marinum ATCC 927, 16S ribosomal RNA gene, partial sequenceU92088880ena1781
20218Mycobacterium marinum strain CIP 104528 16S ribosomal RNA gene, partial sequenceAF547946554ena1781
20218Mycobacterium marinum 16S rRNA geneAJ5360321410ena1781
20218Mycobacterium marinum DNA for 16S rRNA, internal transcribed spacer, 23S rRNAAB026701432ena1781
20218Mycobacterium marinum 16S ribosomal RNA gene, partial sequenceAF271347450ena1781

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Mycobacterium marinum CCUG20998GCA_003391395completencbi1781
66792Mycobacterium marinum ATCC 927GCA_003609695completencbi1781
66792Mycobacterium marinum strain CCUG209981781.42completepatric1781
66792Mycobacterium marinum strain DSM 443441781.47wgspatric1781
66792Mycobacterium marinum strain NCTC22751781.48wgspatric1781
66792Mycobacterium marinum ATCC 9272875591110completeimg1781
66792Mycobacterium marinum CCUG209982860157555completeimg1781
67770Mycobacterium marinum DSM 44344GCA_003431645scaffoldncbi1781
67770Mycobacterium marinum NCTC2275GCA_003431655scaffoldncbi1781

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno87no
flagellatedno98.565no
gram-positiveyes83.837no
anaerobicno98.507no
aerobicyes86.144no
halophileno82.124no
spore-formingno87.507no
glucose-utilyes72.337no
thermophileno98.086no
motileno95.387no
glucose-fermentno92.483yes

External links

@ref: 11690

culture collection no.: DSM 44344, ATCC 927, NCIMB 1297, NCMB 1303, NCTC 2275, TMC 1218, JCM 17638, CCUG 20998, CCUG 27843, CECT 3024, CECT 7091, CIP 104528, IFM 10630, JCM 12275, NCMB 1476, DSM 43225

straininfo link

  • @ref: 77675
  • straininfo: 34648

literature

topicPubmed-IDtitleauthorsjournalyearmeshtopic2DOI
Enzymology2548362Studies on adenylate kinase (ATP:AMP phosphotransferase) of Mycobacterium marinum (ATCC 927).Batra PP, Takeda K, Kreye WActa Leprol1989Adenine Nucleotides/metabolism, Adenylate Kinase/antagonists & inhibitors/*isolation & purification/metabolism, Amino Acids/analysis, Binding Sites, Dinucleoside Phosphates/pharmacology, Energy Metabolism, Molecular Weight, Mycobacterium/*enzymology, Nontuberculous Mycobacteria/*enzymology, Phosphotransferases/*isolation & purification, Protein ConformationMetabolism
Enzymology3004585Purification and characterization of ATP:AMP phosphotransferase from Mycobacterium marinum.Batra PP, Burnette B, Takeda KBiochim Biophys Acta1986Adenylate Kinase/*isolation & purification, Chromatography, Chromatography, DEAE-Cellulose, Chromatography, Gel, Chromatography, Ion Exchange, Drug Stability, Electrophoresis, Polyacrylamide Gel, Mycobacterium/*enzymology, Phosphotransferases/*isolation & purificationPhylogeny10.1016/0167-4838(86)90075-0
Phylogeny12406715Strain variation in Mycobacterium marinum fish isolates.Ucko M, Colorni A, Kvitt H, Diamant A, Zlotkin A, Knibb WRAppl Environ Microbiol2002Animals, *Bacterial Proteins, Chaperonin 60, Chaperonins/*classification/genetics, DNA, Bacterial/analysis, Fishes/*microbiology, Genetic Variation, Mycobacterium marinum/*classification/genetics/isolation & purification, Phylogeny, RNA, Ribosomal, 16S/*analysis/genetics, Restriction MappingEnzymology10.1128/AEM.68.11.5281-5287.2002
Enzymology18500027Biochemical, molecular, and virulence characteristics of select Mycobacterium marinum isolates in hybrid striped bass Morone chrysops x M. saxatilis and zebrafish Danio rerio.Ostland VE, Watral V, Whipps CM, Austin FW, St-Hilaire S, Westerman ME, Kent MLDis Aquat Organ2008Animals, Bass/*microbiology, Crosses, Genetic, DNA, Ribosomal Spacer/genetics, Fish Diseases/*microbiology/mortality/pathology, Heat-Shock Proteins/genetics, Hybridization, Genetic, Mycobacterium Infections, Nontuberculous/microbiology/mortality/pathology/*veterinary, Mycobacterium marinum/genetics/*isolation & purification/*pathogenicity, Spleen/microbiology, Virulence/genetics, Zebrafish/*microbiologyPhylogeny10.3354/dao01891
Stress19531062Husbandry stress exacerbates mycobacterial infections in adult zebrafish, Danio rerio (Hamilton).Ramsay JM, Watral V, Schreck CB, Kent MLJ Fish Dis2009Animals, Fish Diseases/epidemiology/*microbiology/mortality/pathology/*physiopathology, *Fisheries, Hydrocortisone/metabolism, Microsporidia/physiology, Microsporidiosis/microbiology/veterinary, Mycobacterium/*physiology, Mycobacterium Infections/epidemiology/mortality/pathology/physiopathology/*veterinary, Prevalence, *Stress, Physiological, Survival Analysis, Time Factors, Zebrafish/*physiologyMetabolism10.1111/j.1365-2761.2009.01074.x
Genetics29773624Complete Genome Sequence of Mycobacterium marinum ATCC 927(T), Obtained Using Nanopore and Illumina Sequencing Technologies.Yoshida M, Fukano H, Miyamoto Y, Shibayama K, Suzuki M, Hoshino YGenome Announc201810.1128/genomeA.00397-18
34156290Surface-Shaving Proteomics of Mycobacterium marinum Identifies Biofilm Subtype-Specific Changes Affecting Virulence, Tolerance, and Persistence.Savijoki K, Myllymaki H, Luukinen H, Paulamaki L, Vanha-Aho LM, Svorjova A, Miettinen I, Fallarero A, Ihalainen TO, Yli-Kauhaluoma J, Nyman TA, Parikka MmSystems202110.1128/mSystems.00500-21

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
11690Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 44344)https://www.dsmz.de/collection/catalogue/details/culture/DSM-44344
20047Wink, J.http://www.dsmz.de/microorganisms/wink_pdf/DSM44344.pdfCompendium of Actinobacteria. HZI-Helmholtz-Centre for Infection Research, Braunschweig
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
36349Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/16447
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68379Automatically annotated from API Coryne
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
77675Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID34648.1StrainInfo: A central database for resolving microbial strain identifiers
120812Curators of the CIPCollection of Institut Pasteur (CIP 104528)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20104528