Strain identifier
BacDive ID: 8247
Type strain: 
Species: Mycobacterium flavescens
Strain Designation: D-25 (TMC 1541)
Strain history: <- ATCC; ATCC 14474 <- L. F. Bojalil; D-25 (TMC 1541)
NCBI tax ID(s): 1776 (species)
version 7.1 (current version)
General
@ref: 11441
BacDive-ID: 8247
DSM-Number: 43991
keywords: 16S sequence, Bacteria, mesophilic, human pathogen
description: Mycobacterium flavescens D-25 is a mesophilic human pathogen that was isolated from guinea pigs with tuberculosis arrested by chemotherapy.
NCBI tax id
- NCBI tax id: 1776
- Matching level: species
strain history: <- ATCC; ATCC 14474 <- L. F. Bojalil; D-25 (TMC 1541)
doi: 10.13145/bacdive8247.20221219.7.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/actinomycetota
- domain: Bacteria
- phylum: Actinomycetota
- class: Actinomycetes
- order: Mycobacteriales
- family: Mycobacteriaceae
- genus: Mycobacterium
- species: Mycobacterium flavescens
- full scientific name: Mycobacterium flavescens Bojalil et al. 1962 (Approved Lists 1980)
synonyms
- @ref: 20215
- synonym: Mycolicibacterium flavescens
@ref: 11441
domain: Bacteria
phylum: Actinobacteria
class: Actinobacteria
order: Actinomycetales
family: Mycobacteriaceae
genus: Mycobacterium
species: Mycobacterium flavescens
full scientific name: Mycobacterium flavescens Bojalil et al. 1962
strain designation: D-25 (TMC 1541)
type strain: yes
Morphology
cell morphology
- @ref: 69480
- gram stain: positive
- confidence: 97.21
multimedia
| @ref | multimedia content | intellectual property rights | caption |
|---|---|---|---|
| 11441 | https://www.dsmz.de/microorganisms/photos/DSM_43991-1.jpg | © Leibniz-Institut DSMZ | |
| 11441 | https://www.dsmz.de/microorganisms/photos/DSM_43991.jpg | © Leibniz-Institut DSMZ | Medium 250 37°C |
Culture and growth conditions
culture medium
| @ref | name | growth | link | composition |
|---|---|---|---|---|
| 11441 | MIDDLEBROOK MEDIUM (DSMZ Medium 645) | yes | https://mediadive.dsmz.de/medium/645 | Name: MIDDLEBROOK MEDIUM (DSMZ Medium 645) Composition: Bacto Middlebrook 7H10 agar 20.9945 g/l Glycerol Distilled water |
| 11441 | LÖWENSTEIN-JENSEN MEDIUM (DSMZ Medium 354) | yes | https://mediadive.dsmz.de/medium/354 | Name: LÖWENSTEIN-JENSEN MEDIUM (DSMZ Medium 354) Composition: Potato flour 18.6104 g/l L-Asparagin 2.23325 g/l KH2PO4 1.55087 g/l Mg-citrate 0.372208 g/l Malachite green 0.248139 g/l MgSO4 0.148883 g/l Glycerol Fresh egg mixture Distilled water |
| 42099 | MEDIUM 55 - for Mycobacterium | yes |
culture temp
| @ref | growth | type | temperature | range |
|---|---|---|---|---|
| 11441 | positive | growth | 37 | mesophilic |
| 42099 | positive | growth | 37 | mesophilic |
Physiology and metabolism
spore formation
- @ref: 69480
- spore formation: no
- confidence: 93.397
halophily
- @ref: 69480
- halophily level: non-halophilic
- confidence: 96.91
fatty acid profile
fatty acids
@ref fatty acid percentage ECL 50870 C14:0 4.5 14 50870 C16:0 33.7 16 50870 C18:0 1.6 18 50870 C16:1 ω7c 2.5 15.819 50870 C16:1 ω7c/C15:0 ISO 2OH 11.4 15.85 50870 C16:1 ω9c 0.7 15.774 50870 C17:1 ω7c 6.9 16.818 50870 C18:1 ω9c 17 17.769 50870 C18:2 ω6,9c/C18:0 ANTE 2.1 17.724 50870 C20:0 alcohol (C20:0 2OH alc) 10 18.835 50870 TBSA 10Me18:0 5.8 18.392 50870 Unidentified 3.9 18.878 - type of FA analysis: whole cell analysis
- method/protocol: CCUG
Isolation, sampling and environmental information
isolation
| @ref | sample type | sampling date | geographic location | country | origin.country | continent |
|---|---|---|---|---|---|---|
| 11441 | guinea pigs with tuberculosis arrested by chemotherapy | |||||
| 50870 | 1993-10-01 | Göteborg | Sweden | SWE | Europe |
isolation source categories
| Cat1 | Cat2 | Cat3 |
|---|---|---|
| #Infection | #Disease | #Tuberculosis |
| #Host | #Mammals | #Rodentia (Other) |
Safety information
risk assessment
- @ref: 11441
- pathogenicity human: yes
- pathogenicity animal: yes
- biosafety level: 2
- biosafety level comment: Risk group (German classification)
Sequence information
16S sequences
| @ref | description | accession | length | database | NCBI tax ID |
|---|---|---|---|---|---|
| 20218 | Mycobacterium flavescens strain ATCC 14474 16S ribosomal RNA gene, partial sequence | AY734991 | 1279 | ena | 1776 |
| 20218 | Mycobacterium flavescens strain ATCC 14474 16S ribosomal RNA gene, partial sequence | JQ348117 | 210 | ena | 1776 |
| 20218 | M.flavescens 16S ribosomal RNA | M29561 | 1357 | ena | 1776 |
| 20218 | M.flavescens 16S ribosomal RNA gene | M59279 | 146 | ena | 1776 |
| 20218 | Mycobacterium flavescens 16S rRNA gene | X52932 | 1454 | ena | 1776 |
Genome sequences
- @ref: 66792
- description: Mycolicibacterium flavescens DSM 43991
- accession: GCA_025822705
- assembly level: scaffold
- database: ncbi
- NCBI tax ID: 1776
External links
@ref: 11441
culture collection no.: DSM 43991, ATCC 14474, TMC 1541, CCUG 29041, CCUG 32167
straininfo link
| @ref | passport |
|---|---|
| 20218 | http://www.straininfo.net/strains/152994 |
| 20218 | http://www.straininfo.net/strains/97609 |
| 20218 | http://www.straininfo.net/strains/97611 |
literature
- topic: Enzymology
- Pubmed-ID: 15722145
- title: Multicenter evaluation of mycobacteria identification by PCR restriction enzyme analysis in laboratories from Latin America and the Caribbean.
- authors: Leao SC, Bernardelli A, Cataldi A, Zumarraga M, Robledo J, Realpe T, Mejia GI, da Silva Telles MA, Chimara E, Velazco M, Fernandez J, Rodrigues PA, Guerrero MI, Leon CI, Porras TB, Rastogi N, Goh KS, Suffys P, da Silva Rocha A, dos Santos Netto D, Ritacco V, Lopez B, Barrera L, Palomino JC, Martin A, Portaels F
- journal: J Microbiol Methods
- DOI: 10.1016/j.mimet.2004.11.015
- year: 2004
- mesh: Bacterial Proteins/chemistry/genetics, Chaperonin 60, Chaperonins/chemistry/genetics, DNA, Bacterial/chemistry/genetics, Guadeloupe, Humans, Mycobacterium/genetics/*isolation & purification, Mycobacterium Infections/*diagnosis, Polymerase Chain Reaction/*methods/standards, Restriction Mapping, South America
- topic2: Pathogenicity
Reference
| @id | authors | catalogue | doi/url | title | journal | pubmed |
|---|---|---|---|---|---|---|
| 11441 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 43991) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-43991 | |||
| 20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |||
| 20218 | Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P. | 10.1016/j.syapm.2013.11.002 | StrainInfo introduces electronic passports for microorganisms. | Syst Appl Microbiol. 37: 42-50 2014 | 24321274 | |
| 42099 | Curators of the CIP | https://brclims.pasteur.fr/brcWeb/souche/detail/1/16452 | ||||
| 50870 | Curators of the CCUG | Culture Collection University of Gothenburg (CCUG) (CCUG 32167) | https://www.ccug.se/strain?id=32167 | |||
| 66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |||
| 69480 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) |