Strain identifier

BacDive ID: 8192

Type strain: Yes

Species: Psychrobacter pulmonis

Strain Designation: S-606

Strain history: CIP <- 2003, CCUG <- 2002, CECT <- JF Fernandez-Garayzabal, Madrid, Spain: strain S-606

NCBI tax ID(s): 228654 (species)

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General

@ref: 6330

BacDive-ID: 8192

DSM-Number: 16214

keywords: 16S sequence, Bacteria, aerobe, mesophilic, Gram-negative, oval-shaped, animal pathogen

description: Psychrobacter pulmonis S-606 is an aerobe, mesophilic, Gram-negative animal pathogen that was isolated from 20-day-old lamb .

NCBI tax id

  • NCBI tax id: 228654
  • Matching level: species

strain history

@refhistory
6330<- CCUG <- CECT <- J. F. Fernández-Garayzábal, Univ. Complutense, Madrid, Spain <- M. V. Latre, Univ. Zaragoza, Spain; S-606
122048CIP <- 2003, CCUG <- 2002, CECT <- JF Fernandez-Garayzabal, Madrid, Spain: strain S-606

doi: 10.13145/bacdive8192.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Pseudomonadales
  • family: Moraxellaceae
  • genus: Psychrobacter
  • species: Psychrobacter pulmonis
  • full scientific name: Psychrobacter pulmonis Vela et al. 2003

@ref: 6330

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Pseudomonadales

family: Moraxellaceae

genus: Psychrobacter

species: Psychrobacter pulmonis

full scientific name: Psychrobacter pulmonis Vela et al. 2003

strain designation: S-606

type strain: yes

Morphology

cell morphology

  • @ref: 122048
  • gram stain: negative
  • cell shape: oval-shaped
  • motility: no

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
6330COLUMBIA BLOOD AGAR (DSMZ Medium 429)yeshttps://mediadive.dsmz.de/medium/429Name: COLUMBIA BLOOD AGAR (DSMZ Medium 429) Composition: Horse blood 40.0 g/l Columbia agar base
6330BHI MEDIUM (DSMZ Medium 215)yeshttps://mediadive.dsmz.de/medium/215Name: BHI MEDIUM (DSMZ Medium 215) Composition: Brain heart infusion 37.0 g/l Distilled water
34297MEDIUM 6 - Columbia agar with 10 % horse bloodyesDistilled water make up to (1000.000 ml);Columbia agar (39.000 g);Horseblood (100.000 ml)
122048CIP Medium 6yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=6

culture temp

@refgrowthtypetemperaturerange
6330positivegrowth37mesophilic
34297positivegrowth37mesophilic
57122positivegrowth37mesophilic
122048positivegrowth5-37
122048nogrowth41thermophilic

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
57122aerobe
122048obligate aerobe

halophily

  • @ref: 122048
  • salt: NaCl
  • growth: positive
  • tested relation: growth
  • concentration: 0-10 %

metabolite utilization

@refmetaboliteutilization activitykind of utilization testedChebi-ID
68371Potassium 5-ketogluconate-builds acid from
68371Potassium 2-ketogluconate-builds acid from
68371gluconate-builds acid from24265
68371L-arabitol-builds acid from18403
68371D-arabitol-builds acid from18333
68371L-fucose-builds acid from18287
68371D-fucose-builds acid from28847
68371D-lyxose-builds acid from62318
68371turanose-builds acid from32528
68371xylitol-builds acid from17151
68371glycogen-builds acid from28087
68371starch-builds acid from28017
68371raffinose-builds acid from16634
68371melezitose-builds acid from6731
68371inulin-builds acid from15443
68371trehalose-builds acid from27082
68371sucrose-builds acid from17992
68371melibiose-builds acid from28053
68371lactose-builds acid from17716
68371maltose-builds acid from17306
68371cellobiose-builds acid from17057
68371salicin-builds acid from17814
68371esculin-builds acid from4853
68371amygdalin-builds acid from27613
68371N-acetylglucosamine-builds acid from59640
68371methyl alpha-D-glucopyranoside-builds acid from320061
68371methyl alpha-D-mannoside-builds acid from43943
68371D-sorbitol-builds acid from17924
68371D-mannitol-builds acid from16899
68371myo-inositol-builds acid from17268
68371galactitol-builds acid from16813
68371L-rhamnose-builds acid from62345
68371L-sorbose-builds acid from17266
68371D-mannose-builds acid from16024
68371D-fructose-builds acid from15824
68371D-glucose-builds acid from17634
68371D-galactose-builds acid from12936
68371methyl beta-D-xylopyranoside-builds acid from74863
68371ribitol-builds acid from15963
68371L-xylose-builds acid from65328
68371D-ribose-builds acid from16988
68371L-arabinose-builds acid from30849
68371D-arabinose-builds acid from17108
68371erythritol-builds acid from17113
68371glycerol-builds acid from17754
122048citrate-carbon source16947
122048esculin-hydrolysis4853
122048nitrate+reduction17632
122048nitrite-reduction16301
122048nitrate-respiration17632

antibiotic resistance

  • @ref: 122048
  • metabolite: 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)
  • is antibiotic: yes
  • is sensitive: no
  • is resistant: yes

metabolite production

  • @ref: 122048
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testmethylred-test
12204815688acetoin+
12204817234glucose-

enzymes

@refvalueactivityec
122048oxidase+
122048beta-galactosidase-3.2.1.23
122048alcohol dehydrogenase-1.1.1.1
122048gelatinase-
122048amylase-
122048DNase-
122048caseinase-3.4.21.50
122048catalase+1.11.1.6
122048tween esterase-
122048gamma-glutamyltransferase+2.3.2.2
122048lecithinase+
122048lipase+
122048lysine decarboxylase-4.1.1.18
122048ornithine decarboxylase-4.1.1.17
122048phenylalanine ammonia-lyase+4.3.1.24
122048protease-
122048tryptophan deaminase+
122048urease-3.5.1.5
68382alkaline phosphatase+3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)+
68382lipase (C 14)+
68382leucine arylamidase+3.4.11.1
68382valine arylamidase+
68382cystine arylamidase+3.4.11.3
68382trypsin-3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase-3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase+3.2.1.20
68382beta-glucosidase-3.2.1.21
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
122048-+++++++--++---+----

API 50CHac

@refGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
122048-----+/------------------+/---------------+/---+/--------

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinentsampling dateisolation date
633020-day-old lamb (congestive lungs)ZaragozaSpainESPEurope
57122Congestive lungs of 20-days-old lamb,sudden deathZaragozaSpainESPEurope2001
122048Lambs, lungsZaragozaSpainESPEurope2001

isolation source categories

  • Cat1: #Host
  • Cat2: #Mammals
  • Cat3: #Caprinae (Sheep/Goat)

taxonmaps

  • @ref: 69479
  • File name: preview.99_3096.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_80;96_193;97_209;98_234;99_3096&stattab=map
  • Last taxonomy: Psychrobacter
  • 16S sequence: HQ698579
  • Sequence Identity:
  • Total samples: 2999
  • soil counts: 382
  • aquatic counts: 1079
  • animal counts: 1459
  • plant counts: 79

Safety information

risk assessment

@refpathogenicity animalbiosafety levelbiosafety level comment
6330yes2Risk group (German classification)
1220481Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Psychrobacter pulmonis strain CCUG 46240 16S ribosomal RNA gene, partial sequenceHQ6985791469ena228654
6330Psychrobacter pulmonis 16S rRNA gene, type strain CECT 5989TAJ4376961363ena228654

External links

@ref: 6330

culture collection no.: DSM 16214, CCUG 46240, CECT 5989, CIP 107927, CCM 7211

straininfo link

  • @ref: 77595
  • straininfo: 97532

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny12710606Psychrobacter pulmonis sp. nov., isolated from the lungs of lambs.Vela AI, Collins MD, Latre MV, Mateos A, Moreno MA, Hutson R, Dominguez L, Fernandez-Garayzabal JFInt J Syst Evol Microbiol10.1099/ijs.0.02413-02003Animals, Gammaproteobacteria/chemistry/*classification/genetics/physiology, Gram-Negative Bacterial Infections/microbiology/*veterinary, Lung/*microbiology, Molecular Sequence Data, Phylogeny, RNA, Ribosomal, 16S/*analysis/genetics, Sequence Analysis, DNA, Sheep, Sheep Diseases/microbiologyGenetics
Phylogeny19502339Psychrobacter fulvigenes sp. nov., isolated from a marine crustacean from the Sea of Japan.Romanenko LA, Tanaka N, Frolova GM, Mikhailov VVInt J Syst Evol Microbiol10.1099/ijs.0.007195-02009Animals, Bacterial Typing Techniques, Base Composition, Crustacea/*microbiology, DNA Gyrase/genetics, DNA, Bacterial/analysis, DNA, Ribosomal/analysis, Japan, Molecular Sequence Data, Nucleic Acid Hybridization, Oceans and Seas, Phenotype, Phylogeny, Polymerase Chain Reaction/methods, Psychrobacter/*classification/genetics/*isolation & purification/physiology, RNA, Ribosomal, 16S/genetics, Seawater, Sequence Analysis, DNA, Species SpecificityGenetics
Pathogenicity33401388AhaP, A Quorum Quenching Acylase from Psychrobacter sp. M9-54-1 That Attenuates Pseudomonas aeruginosa and Vibrio coralliilyticus Virulence.Reina JC, Romero M, Salto R, Camara M, Llamas IMar Drugs10.3390/md1901001620214-Butyrolactone/analogs & derivatives/metabolism, Acyl-Butyrolactones/metabolism, Amidohydrolases/*pharmacology, Animals, Artemia, Genome, Bacterial/genetics, Molecular Structure, Pseudomonas aeruginosa/*drug effects/*pathogenicity, Psychrobacter/*chemistry/genetics, Quorum Sensing/*drug effects, RNA, Ribosomal, 16S, Vibrio/*drug effects/*pathogenicity, Virulence/*drug effectsMetabolism

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
6330Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 16214)https://www.dsmz.de/collection/catalogue/details/culture/DSM-16214
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
34297Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/5492
57122Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 46240)https://www.ccug.se/strain?id=46240
68371Automatically annotated from API 50CH acid
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
77595Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID97532.1StrainInfo: A central database for resolving microbial strain identifiers
122048Curators of the CIPCollection of Institut Pasteur (CIP 107927)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20107927