Strain identifier
BacDive ID: 8187
Type strain:
Species: Psychrobacter maritimus
Strain Designation: Pi 2-20
Strain history: CIP <- 2005, DSMZ <- L. A. Romanenko: strain Pi 2-20
NCBI tax ID(s): 256325 (species)
General
@ref: 5888
BacDive-ID: 8187
DSM-Number: 15387
keywords: 16S sequence, Bacteria, aerobe, mesophilic, Gram-negative, ovoid-shaped
description: Psychrobacter maritimus Pi 2-20 is an aerobe, mesophilic, Gram-negative bacterium that was isolated from sea-ice sample.
NCBI tax id
- NCBI tax id: 256325
- Matching level: species
strain history
@ref | history |
---|---|
5888 | <- L. A. Romanenko; Pi 2-20 |
120839 | CIP <- 2005, DSMZ <- L. A. Romanenko: strain Pi 2-20 |
doi: 10.13145/bacdive8187.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/pseudomonadota
- domain: Bacteria
- phylum: Pseudomonadota
- class: Gammaproteobacteria
- order: Pseudomonadales
- family: Moraxellaceae
- genus: Psychrobacter
- species: Psychrobacter maritimus
- full scientific name: Psychrobacter maritimus Romanenko et al. 2004
@ref: 5888
domain: Bacteria
phylum: Proteobacteria
class: Gammaproteobacteria
order: Pseudomonadales
family: Moraxellaceae
genus: Psychrobacter
species: Psychrobacter maritimus
full scientific name: Psychrobacter maritimus Romanenko et al. 2004
strain designation: Pi 2-20
type strain: yes
Morphology
cell morphology
@ref | gram stain | cell length | cell width | cell shape | motility |
---|---|---|---|---|---|
31230 | negative | 1.55 µm | 1.25 µm | ovoid-shaped | no |
120839 | negative | coccus-shaped | no |
pigmentation
- @ref: 31230
- production: no
Culture and growth conditions
culture medium
@ref | name | growth | link | composition |
---|---|---|---|---|
5888 | BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514) | yes | https://mediadive.dsmz.de/medium/514 | Name: BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514) Composition: NaCl 19.45 g/l MgCl2 5.9 g/l Bacto peptone 5.0 g/l Na2SO4 3.24 g/l CaCl2 1.8 g/l Yeast extract 1.0 g/l KCl 0.55 g/l NaHCO3 0.16 g/l Fe(III) citrate 0.1 g/l KBr 0.08 g/l SrCl2 0.034 g/l H3BO3 0.022 g/l Na2HPO4 0.008 g/l Na-silicate 0.004 g/l NaF 0.0024 g/l (NH4)NO3 0.0016 g/l Distilled water |
38319 | Marine agar (MA) | yes | Distilled water make up to (1000.000 ml);Marine agar (55.100 g) | |
120839 | CIP Medium 72 | yes | https://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=72 | |
120839 | CIP Medium 13 | yes | https://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=13 |
culture temp
@ref | growth | type | temperature | range |
---|---|---|---|---|
5888 | positive | growth | 25-28 | mesophilic |
31230 | positive | growth | 04-37 | |
31230 | positive | optimum | 26.5 | mesophilic |
38319 | positive | growth | 25 | mesophilic |
120839 | positive | growth | 5-30 | |
120839 | no | growth | 37 | mesophilic |
120839 | no | growth | 41 | thermophilic |
culture pH
@ref | ability | type | pH | PH range |
---|---|---|---|---|
31230 | positive | growth | 05-10 | alkaliphile |
31230 | positive | optimum | 7.25 |
Physiology and metabolism
oxygen tolerance
@ref | oxygen tolerance |
---|---|
31230 | aerobe |
120839 | obligate aerobe |
spore formation
- @ref: 31230
- spore formation: no
halophily
@ref | salt | growth | tested relation | concentration |
---|---|---|---|---|
31230 | NaCl | positive | growth | 0-10 % |
31230 | NaCl | positive | optimum | 5 % |
120839 | NaCl | positive | growth | 0-10 % |
observation
- @ref: 31230
- observation: aggregates in chains
metabolite utilization
@ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
---|---|---|---|---|
31230 | 30089 | acetate | + | carbon source |
31230 | 28644 | 2-oxopentanoate | + | carbon source |
31230 | 29987 | glutamate | + | carbon source |
31230 | 25017 | leucine | + | carbon source |
31230 | 18257 | ornithine | + | carbon source |
31230 | 53423 | tween 40 | + | carbon source |
31230 | 53426 | tween 80 | + | carbon source |
31230 | 17632 | nitrate | + | reduction |
120839 | 16947 | citrate | - | carbon source |
120839 | 4853 | esculin | - | hydrolysis |
120839 | 17632 | nitrate | + | reduction |
120839 | 16301 | nitrite | + | reduction |
120839 | 17632 | nitrate | - | respiration |
metabolite production
- @ref: 120839
- Chebi-ID: 35581
- metabolite: indole
- production: no
metabolite tests
@ref | Chebi-ID | metabolite | voges-proskauer-test | methylred-test |
---|---|---|---|---|
120839 | 15688 | acetoin | - | |
120839 | 17234 | glucose | - |
enzymes
@ref | value | activity | ec |
---|---|---|---|
31230 | catalase | + | 1.11.1.6 |
31230 | cytochrome oxidase | + | 1.9.3.1 |
31230 | urease | + | 3.5.1.5 |
120839 | oxidase | + | |
120839 | beta-galactosidase | - | 3.2.1.23 |
120839 | alcohol dehydrogenase | - | 1.1.1.1 |
120839 | gelatinase | - | |
120839 | amylase | - | |
120839 | DNase | - | |
120839 | caseinase | - | 3.4.21.50 |
120839 | catalase | + | 1.11.1.6 |
120839 | tween esterase | + | |
120839 | gamma-glutamyltransferase | + | 2.3.2.2 |
120839 | lecithinase | - | |
120839 | lipase | + | |
120839 | lysine decarboxylase | - | 4.1.1.18 |
120839 | ornithine decarboxylase | - | 4.1.1.17 |
120839 | phenylalanine ammonia-lyase | + | 4.3.1.24 |
120839 | protease | - | |
120839 | tryptophan deaminase | - | |
120839 | urease | + | 3.5.1.5 |
68382 | alkaline phosphatase | + | 3.1.3.1 |
68382 | esterase (C 4) | + | |
68382 | esterase lipase (C 8) | + | |
68382 | lipase (C 14) | + | |
68382 | leucine arylamidase | + | 3.4.11.1 |
68382 | valine arylamidase | - | |
68382 | cystine arylamidase | - | 3.4.11.3 |
68382 | trypsin | - | 3.4.21.4 |
68382 | alpha-chymotrypsin | - | 3.4.21.1 |
68382 | acid phosphatase | - | 3.1.3.2 |
68382 | naphthol-AS-BI-phosphohydrolase | + | |
68382 | alpha-galactosidase | - | 3.2.1.22 |
68382 | beta-galactosidase | - | 3.2.1.23 |
68382 | beta-glucuronidase | - | 3.2.1.31 |
68382 | alpha-glucosidase | - | 3.2.1.20 |
68382 | beta-glucosidase | - | 3.2.1.21 |
68382 | N-acetyl-beta-glucosaminidase | - | 3.2.1.52 |
68382 | alpha-mannosidase | - | 3.2.1.24 |
68382 | alpha-fucosidase | - | 3.2.1.51 |
API zym
@ref | Control | Alkaline phosphatase | Esterase | Esterase Lipase | Lipase | Leucine arylamidase | Valine arylamidase | Cystine arylamidase | Trypsin | alpha- Chymotrypsin | Acid phosphatase | Naphthol-AS-BI-phosphohydrolase | alpha- Galactosidase | beta- Galactosidase | beta- Glucuronidase | alpha- Glucosidase | beta- Glucosidase | N-acetyl-beta- glucosaminidase | alpha- Mannosidase | alpha- Fucosidase |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
120839 | - | + | + | + | + | + | - | - | - | - | - | + | - | - | - | - | - | - | - | - |
Isolation, sampling and environmental information
isolation
@ref | sample type | geographic location | country | origin.country | continent |
---|---|---|---|---|---|
5888 | sea-ice sample | Sea of Japan | Russia | RUS | Asia |
120839 | Environment, Sea-ice sample | Russian Federation | RUS | Europe |
isolation source categories
Cat1 | Cat2 | Cat3 |
---|---|---|
#Environmental | #Aquatic | #Ice |
#Environmental | #Aquatic | #Marine |
taxonmaps
- @ref: 69479
- File name: preview.99_3096.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_80;96_193;97_209;98_234;99_3096&stattab=map
- Last taxonomy: Psychrobacter
- 16S sequence: AJ609272
- Sequence Identity:
- Total samples: 2999
- soil counts: 382
- aquatic counts: 1079
- animal counts: 1459
- plant counts: 79
Safety information
risk assessment
@ref | biosafety level | biosafety level comment |
---|---|---|
5888 | 1 | Risk group (German classification) |
120839 | 1 | Risk group (French classification) |
Sequence information
16S sequences
- @ref: 5888
- description: Psychrobacter maritimus 16S rRNA gene, type strain Pi2-20T
- accession: AJ609272
- length: 1476
- database: ena
- NCBI tax ID: 256325
GC content
- @ref: 31230
- GC-content: 44.75
External links
@ref: 5888
culture collection no.: DSM 15387, CIP 108811, KMM 3646
straininfo link
- @ref: 77590
- straininfo: 127682
literature
- topic: Phylogeny
- Pubmed-ID: 15388738
- title: Psychrobacter maritimus sp. nov. and Psychrobacter arenosus sp. nov., isolated from coastal sea ice and sediments of the Sea of Japan.
- authors: Romanenko LA, Lysenko AM, Rohde M, Mikhailov VV, Stackebrandt E
- journal: Int J Syst Evol Microbiol
- DOI: 10.1099/ijs.0.63096-0
- year: 2004
- mesh: Anti-Bacterial Agents/pharmacology, Bacterial Typing Techniques, DNA, Bacterial/chemistry/isolation & purification, DNA, Ribosomal/chemistry/isolation & purification, Fatty Acids/analysis, Genes, rRNA, Geologic Sediments/*microbiology, *Ice, Japan, Molecular Sequence Data, Movement, Phylogeny, Pigments, Biological/biosynthesis, Psychrobacter/*classification/cytology/*isolation & purification/physiology, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/genetics, Saline Solution, Hypertonic/pharmacology, Sequence Analysis, DNA, Temperature, Water Microbiology
- topic2: Enzymology
Reference
@id | authors | catalogue | doi/url | title | ID_cross_reference | pubmed |
---|---|---|---|---|---|---|
5888 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 15387) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-15387 | |||
20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |||
31230 | Barberan A, Caceres Velazquez H, Jones S, Fierer N. | 10.1128/mSphere.00237-17 | Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information | 27555 | 28776041 | |
38319 | Curators of the CIP | https://brclims.pasteur.fr/brcWeb/souche/detail/1/6481 | ||||
68382 | Automatically annotated from API zym | |||||
69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | https://microbeatlas.org/ | MicrobeAtlas 1.0 beta | |||
77590 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID127682.1 | StrainInfo: A central database for resolving microbial strain identifiers | |||
120839 | Curators of the CIP | Collection of Institut Pasteur (CIP 108811) | https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20108811 |