Strain identifier

BacDive ID: 8187

Type strain: Yes

Species: Psychrobacter maritimus

Strain Designation: Pi 2-20

Strain history: CIP <- 2005, DSMZ <- L. A. Romanenko: strain Pi 2-20

NCBI tax ID(s): 256325 (species)

For citation purpose refer to the digital object identifier (doi) of the current version.
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General

@ref: 5888

BacDive-ID: 8187

DSM-Number: 15387

keywords: 16S sequence, Bacteria, aerobe, mesophilic, Gram-negative, ovoid-shaped

description: Psychrobacter maritimus Pi 2-20 is an aerobe, mesophilic, Gram-negative bacterium that was isolated from sea-ice sample.

NCBI tax id

  • NCBI tax id: 256325
  • Matching level: species

strain history

@refhistory
5888<- L. A. Romanenko; Pi 2-20
120839CIP <- 2005, DSMZ <- L. A. Romanenko: strain Pi 2-20

doi: 10.13145/bacdive8187.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Pseudomonadales
  • family: Moraxellaceae
  • genus: Psychrobacter
  • species: Psychrobacter maritimus
  • full scientific name: Psychrobacter maritimus Romanenko et al. 2004

@ref: 5888

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Pseudomonadales

family: Moraxellaceae

genus: Psychrobacter

species: Psychrobacter maritimus

full scientific name: Psychrobacter maritimus Romanenko et al. 2004

strain designation: Pi 2-20

type strain: yes

Morphology

cell morphology

@refgram staincell lengthcell widthcell shapemotility
31230negative1.55 µm1.25 µmovoid-shapedno
120839negativecoccus-shapedno

pigmentation

  • @ref: 31230
  • production: no

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
5888BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514)yeshttps://mediadive.dsmz.de/medium/514Name: BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514) Composition: NaCl 19.45 g/l MgCl2 5.9 g/l Bacto peptone 5.0 g/l Na2SO4 3.24 g/l CaCl2 1.8 g/l Yeast extract 1.0 g/l KCl 0.55 g/l NaHCO3 0.16 g/l Fe(III) citrate 0.1 g/l KBr 0.08 g/l SrCl2 0.034 g/l H3BO3 0.022 g/l Na2HPO4 0.008 g/l Na-silicate 0.004 g/l NaF 0.0024 g/l (NH4)NO3 0.0016 g/l Distilled water
38319Marine agar (MA)yesDistilled water make up to (1000.000 ml);Marine agar (55.100 g)
120839CIP Medium 72yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=72
120839CIP Medium 13yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=13

culture temp

@refgrowthtypetemperaturerange
5888positivegrowth25-28mesophilic
31230positivegrowth04-37
31230positiveoptimum26.5mesophilic
38319positivegrowth25mesophilic
120839positivegrowth5-30
120839nogrowth37mesophilic
120839nogrowth41thermophilic

culture pH

@refabilitytypepHPH range
31230positivegrowth05-10alkaliphile
31230positiveoptimum7.25

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
31230aerobe
120839obligate aerobe

spore formation

  • @ref: 31230
  • spore formation: no

halophily

@refsaltgrowthtested relationconcentration
31230NaClpositivegrowth0-10 %
31230NaClpositiveoptimum5 %
120839NaClpositivegrowth0-10 %

observation

  • @ref: 31230
  • observation: aggregates in chains

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
3123030089acetate+carbon source
31230286442-oxopentanoate+carbon source
3123029987glutamate+carbon source
3123025017leucine+carbon source
3123018257ornithine+carbon source
3123053423tween 40+carbon source
3123053426tween 80+carbon source
3123017632nitrate+reduction
12083916947citrate-carbon source
1208394853esculin-hydrolysis
12083917632nitrate+reduction
12083916301nitrite+reduction
12083917632nitrate-respiration

metabolite production

  • @ref: 120839
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testmethylred-test
12083915688acetoin-
12083917234glucose-

enzymes

@refvalueactivityec
31230catalase+1.11.1.6
31230cytochrome oxidase+1.9.3.1
31230urease+3.5.1.5
120839oxidase+
120839beta-galactosidase-3.2.1.23
120839alcohol dehydrogenase-1.1.1.1
120839gelatinase-
120839amylase-
120839DNase-
120839caseinase-3.4.21.50
120839catalase+1.11.1.6
120839tween esterase+
120839gamma-glutamyltransferase+2.3.2.2
120839lecithinase-
120839lipase+
120839lysine decarboxylase-4.1.1.18
120839ornithine decarboxylase-4.1.1.17
120839phenylalanine ammonia-lyase+4.3.1.24
120839protease-
120839tryptophan deaminase-
120839urease+3.5.1.5
68382alkaline phosphatase+3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)+
68382lipase (C 14)+
68382leucine arylamidase+3.4.11.1
68382valine arylamidase-
68382cystine arylamidase-3.4.11.3
68382trypsin-3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase-3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase-3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase-3.2.1.20
68382beta-glucosidase-3.2.1.21
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
120839-+++++-----+--------

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinent
5888sea-ice sampleSea of JapanRussiaRUSAsia
120839Environment, Sea-ice sampleRussian FederationRUSEurope

isolation source categories

Cat1Cat2Cat3
#Environmental#Aquatic#Ice
#Environmental#Aquatic#Marine

taxonmaps

  • @ref: 69479
  • File name: preview.99_3096.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_80;96_193;97_209;98_234;99_3096&stattab=map
  • Last taxonomy: Psychrobacter
  • 16S sequence: AJ609272
  • Sequence Identity:
  • Total samples: 2999
  • soil counts: 382
  • aquatic counts: 1079
  • animal counts: 1459
  • plant counts: 79

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
58881Risk group (German classification)
1208391Risk group (French classification)

Sequence information

16S sequences

  • @ref: 5888
  • description: Psychrobacter maritimus 16S rRNA gene, type strain Pi2-20T
  • accession: AJ609272
  • length: 1476
  • database: ena
  • NCBI tax ID: 256325

GC content

  • @ref: 31230
  • GC-content: 44.75

External links

@ref: 5888

culture collection no.: DSM 15387, CIP 108811, KMM 3646

straininfo link

  • @ref: 77590
  • straininfo: 127682

literature

  • topic: Phylogeny
  • Pubmed-ID: 15388738
  • title: Psychrobacter maritimus sp. nov. and Psychrobacter arenosus sp. nov., isolated from coastal sea ice and sediments of the Sea of Japan.
  • authors: Romanenko LA, Lysenko AM, Rohde M, Mikhailov VV, Stackebrandt E
  • journal: Int J Syst Evol Microbiol
  • DOI: 10.1099/ijs.0.63096-0
  • year: 2004
  • mesh: Anti-Bacterial Agents/pharmacology, Bacterial Typing Techniques, DNA, Bacterial/chemistry/isolation & purification, DNA, Ribosomal/chemistry/isolation & purification, Fatty Acids/analysis, Genes, rRNA, Geologic Sediments/*microbiology, *Ice, Japan, Molecular Sequence Data, Movement, Phylogeny, Pigments, Biological/biosynthesis, Psychrobacter/*classification/cytology/*isolation & purification/physiology, RNA, Bacterial/genetics, RNA, Ribosomal, 16S/genetics, Saline Solution, Hypertonic/pharmacology, Sequence Analysis, DNA, Temperature, Water Microbiology
  • topic2: Enzymology

Reference

@idauthorscataloguedoi/urltitleID_cross_referencepubmed
5888Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 15387)https://www.dsmz.de/collection/catalogue/details/culture/DSM-15387
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
31230Barberan A, Caceres Velazquez H, Jones S, Fierer N.10.1128/mSphere.00237-17Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information2755528776041
38319Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/6481
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
77590Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID127682.1StrainInfo: A central database for resolving microbial strain identifiers
120839Curators of the CIPCollection of Institut Pasteur (CIP 108811)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20108811