Archive BacDiveID:10.13145/bacdive8186.20221219.7.1

Strain identifier

BacDive ID: 8186

Type strain:

Species: Psychrobacter submarinus

Strain history: <- P. Schumann <- L. A. Romanenko, KMM

NCBI tax ID(s): 154108 (species)

For citation purpose refer to the digital object identifier (doi) of the current version.

General

@ref: 5237

BacDive-ID: 8186

DSM-Number: 14161

keywords: genome sequence, 16S sequence, Bacteria, mesophilic

description: Psychrobacter submarinus DSM 14161 is a mesophilic bacterium that was isolated from seawater at a depth of 300 m.

NCBI tax id

NCBI tax id: 154108
Matching level: species

strain history: <- P. Schumann <- L. A. Romanenko, KMM

doi: 10.13145/bacdive8186.20221219.7.1

Name and taxonomic classification

LPSN

@ref: 20215
description: domain/bacteria
keyword: phylum/pseudomonadota
domain: Bacteria
phylum: Pseudomonadota
class: Gammaproteobacteria
order: Pseudomonadales
family: Moraxellaceae
genus: Psychrobacter
species: Psychrobacter submarinus
full scientific name: Psychrobacter submarinus Romanenko et al. 2002

@ref: 5237

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Pseudomonadales

family: Moraxellaceae

genus: Psychrobacter

species: Psychrobacter submarinus

full scientific name: Psychrobacter submarinus Romanenko et al. 2002

type strain: yes

Morphology

cell morphology

@ref: 69480
gram stain: negative
confidence: 99.768

Culture and growth conditions

culture medium

@ref	name	growth	link	composition
5237	BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514)	yes	https://mediadive.dsmz.de/medium/514	Name: BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514; with strain-specific modifications) Composition: NaCl 30.0 g/l MgCl2 5.9 g/l Bacto peptone 5.0 g/l Na2SO4 3.24 g/l CaCl2 1.8 g/l Yeast extract 1.0 g/l KCl 0.55 g/l NaHCO3 0.16 g/l Fe(III) citrate 0.1 g/l KBr 0.08 g/l SrCl2 0.034 g/l H3BO3 0.022 g/l Na2HPO4 0.008 g/l Na-silicate 0.004 g/l NaF 0.0024 g/l (NH4)NO3 0.0016 g/l Distilled water
33154	Marine agar (MA)	yes		Distilled water make up to (1000.000 ml);Marine agar (55.100 g)

culture temp

@ref	growth	type	temperature	range
5237	positive	growth	25	mesophilic
33154	positive	growth	25	mesophilic

Physiology and metabolism

spore formation

@ref: 69480
spore formation: no
confidence: 98.749

halophily

@ref: 69480
halophily level: non-halophilic
confidence: 92.395

Isolation, sampling and environmental information

isolation

@ref: 5237
sample type: seawater at a depth of 300 m
geographic location: Pacific Ocean 12°41'N, 132°38'W
latitude: 12.6833
longitude: -132.633

isolation source categories

Cat1: #Environmental
Cat2: #Aquatic
Cat3: #Marine

taxonmaps

@ref: 69479
File name: preview.99_1920.png
url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_80;96_193;97_209;98_1507;99_1920&stattab=map
Last taxonomy: Psychrobacter
16S sequence: AJ309940
Sequence Identity:
Total samples: 1598
soil counts: 91
aquatic counts: 1139
animal counts: 350
plant counts: 18

Safety information

risk assessment

@ref: 5237
biosafety level: 1
biosafety level comment: Risk group (German classification)

Sequence information

16S sequences

@ref: 5237
description: Psychrobacter submarinus 16S rRNA gene, strain KMM 225
accession: AJ309940
length: 1428
database: ena
NCBI tax ID: 154108

Genome sequences

@ref	description	accession	assembly level	database	NCBI tax ID
66792	Psychrobacter submarinus Psychrobacter submarinus KMM225	GCA_904846685	contig	ncbi	154108
66792	Psychrobacter submarinus strain Psychrobacter submarinus KMM225	154108.8	wgs	patric	154108
66792	Escherichia coli SE58	GCA_009709945	contig	patric	562

GC content

@ref: 5237
GC-content: 46.7

External links

@ref: 5237

culture collection no.: DSM 14161, KMM 225

straininfo link

@ref	passport
20218	http://www.straininfo.net/strains/303608
20218	http://www.straininfo.net/strains/278528

literature

topic	Pubmed-ID	title	authors	journal	DOI	year	mesh	topic2
Phylogeny	12148642	Psychrobacter submarinus sp. nov. and Psychrobacter marincola sp. nov., psychrophilic halophiles from marine environments.	Romanenko LA, Schumann P, Rohde M, Lysenko AM, Mikhailov VV, Stackebrandt E	Int J Syst Evol Microbiol	10.1099/00207713-52-4-1291	2002	Animals, Bacterial Typing Techniques, Cold Temperature, DNA, Ribosomal/analysis, Gammaproteobacteria/chemistry/classification/genetics/physiology, Genotype, Molecular Sequence Data, Phenotype, Phylogeny, RNA, Ribosomal, 16S/genetics, Seawater/microbiology, Sequence Analysis, DNA, Sodium Chloride/metabolism, Urochordata/*microbiology	Genetics
Pathogenicity	33838818	Structure and in vitro antiproliferative activity of the acidic capsular polysaccharide from the deep-sea bacterium Psychrobacter submarinus KMM 225(T).	Kokoulin MS, Kuzmich AS, Romanenko LA, Chikalovets IV	Carbohydr Polym	10.1016/j.carbpol.2021.117941	2021	Aquatic Organisms/chemistry, Carbohydrate Sequence, Cell Proliferation/drug effects, Cell Survival/drug effects, Humans, K562 Cells, Magnetic Resonance Spectroscopy/methods, Polysaccharides, Bacterial/chemistry/isolation & purification/pharmacology, Psychrobacter/chemistry, Seawater/microbiology	Enzymology

Reference

@id	authors	catalogue	doi/url	title	journal	pubmed
5237	Curators of the DSMZ	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 14161)	https://www.dsmz.de/collection/catalogue/details/culture/DSM-14161
20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.		10.1099/ijsem.0.004332	List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218	Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.		10.1016/j.syapm.2013.11.002	StrainInfo introduces electronic passports for microorganisms.	Syst Appl Microbiol. 37: 42-50 2014	24321274
33154	Curators of the CIP		https://brclims.pasteur.fr/brcWeb/souche/detail/1/5099
66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann		https://diaspora-project.de/progress.html#genomes	Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.		https://microbeatlas.org/	MicrobeAtlas 1.0 beta
69480	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann		https://diaspora-project.de/progress.html#genomes	Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)