Strain identifier

BacDive ID: 8185

Type strain: Yes

Species: Psychrobacter marincola

Strain Designation: 238-6

Strain history: CIP <- 2002, DSMZ <- P. Schumann <- L.A. Romanenko, KMM

NCBI tax ID(s): 154109 (species)

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General

@ref: 5236

BacDive-ID: 8185

DSM-Number: 14160

keywords: 16S sequence, Bacteria, mesophilic, Gram-negative, oval-shaped

description: Psychrobacter marincola 238-6 is a mesophilic, Gram-negative, oval-shaped bacterium that was isolated from internal tissues of the ascidian Polysyncraton sp..

NCBI tax id

  • NCBI tax id: 154109
  • Matching level: species

strain history

@refhistory
5236<- P. Schumann <- L. A. Romanenko, KMM; 238-6
120803CIP <- 2002, DSMZ <- P. Schumann <- L.A. Romanenko, KMM

doi: 10.13145/bacdive8185.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Pseudomonadales
  • family: Moraxellaceae
  • genus: Psychrobacter
  • species: Psychrobacter marincola
  • full scientific name: Psychrobacter marincola Romanenko et al. 2002

@ref: 5236

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Pseudomonadales

family: Moraxellaceae

genus: Psychrobacter

species: Psychrobacter marincola

full scientific name: Psychrobacter marincola Romanenko et al. 2002

strain designation: 238-6

type strain: yes

Morphology

cell morphology

  • @ref: 120803
  • gram stain: negative
  • cell shape: oval-shaped
  • motility: no

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
5236BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514)yeshttps://mediadive.dsmz.de/medium/514Name: BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514) Composition: NaCl 19.45 g/l MgCl2 5.9 g/l Bacto peptone 5.0 g/l Na2SO4 3.24 g/l CaCl2 1.8 g/l Yeast extract 1.0 g/l KCl 0.55 g/l NaHCO3 0.16 g/l Fe(III) citrate 0.1 g/l KBr 0.08 g/l SrCl2 0.034 g/l H3BO3 0.022 g/l Na2HPO4 0.008 g/l Na-silicate 0.004 g/l NaF 0.0024 g/l (NH4)NO3 0.0016 g/l Distilled water
33152Marine agar (MA)yesDistilled water make up to (1000.000 ml);Marine agar (55.100 g)
120803CIP Medium 13yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=13

culture temp

@refgrowthtypetemperaturerange
5236positivegrowth25mesophilic
33152positivegrowth25mesophilic
120803positivegrowth15-30
120803nogrowth5psychrophilic
120803nogrowth37mesophilic
120803nogrowth41thermophilic

Physiology and metabolism

halophily

@refsaltgrowthtested relationconcentration
120803NaClpositivegrowth4-6 %
120803NaClnogrowth0 %
120803NaClnogrowth2 %
120803NaClnogrowth8 %
120803NaClnogrowth10 %

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
12080316947citrate-carbon source
1208034853esculin-hydrolysis
12080317632nitrate-reduction
12080316301nitrite-reduction
12080317632nitrate-respiration

antibiotic resistance

  • @ref: 120803
  • metabolite: 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)
  • is antibiotic: yes
  • is sensitive: no
  • is resistant: yes

metabolite production

  • @ref: 120803
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testmethylred-test
12080315688acetoin-
12080317234glucose-

enzymes

@refvalueactivityec
120803oxidase+
120803beta-galactosidase+3.2.1.23
120803alcohol dehydrogenase-1.1.1.1
120803gelatinase-
120803amylase-
120803caseinase-3.4.21.50
120803catalase+1.11.1.6
120803tween esterase+
120803lecithinase-
120803lipase-
120803lysine decarboxylase-4.1.1.18
120803ornithine decarboxylase-4.1.1.17
120803phenylalanine ammonia-lyase+4.3.1.24
120803protease-
120803tryptophan deaminase+
120803urease-3.5.1.5
68382alkaline phosphatase-3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)+
68382lipase (C 14)-
68382leucine arylamidase+3.4.11.1
68382valine arylamidase-
68382cystine arylamidase-3.4.11.3
68382trypsin-3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase-3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase-3.2.1.20
68382beta-glucosidase-3.2.1.21
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
120803--++-+----++--------

Isolation, sampling and environmental information

isolation

@refsample typehost speciesgeographic locationcontinentlatitudelongitudecountryorigin.countryisolation date
5236internal tissues of the ascidian Polysyncraton sp.PolysyncratonIndian Ocean 33°41'S 134°28'EAsia-33.6833134.467
120803Internal tissues of the ascidian Polysyncraton sp.Indian OceanAsiaIndiaIND1988

isolation source categories

Cat1Cat2Cat3
#Host#Invertebrates (Other)#Tunicata
#Host Body-Site

taxonmaps

  • @ref: 69479
  • File name: preview.99_1920.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_80;96_193;97_209;98_1507;99_1920&stattab=map
  • Last taxonomy: Psychrobacter
  • 16S sequence: AJ309941
  • Sequence Identity:
  • Total samples: 1598
  • soil counts: 91
  • aquatic counts: 1139
  • animal counts: 350
  • plant counts: 18

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
52361Risk group (German classification)
1208031Risk group (French classification)

Sequence information

16S sequences

  • @ref: 5236
  • description: Psychrobacter marincola 16S rRNA gene, strain KMM 277
  • accession: AJ309941
  • length: 1428
  • database: ena
  • NCBI tax ID: 154109

GC content

  • @ref: 5236
  • GC-content: 50.7

External links

@ref: 5236

culture collection no.: DSM 14160, KMM 277, CIP 107640

straininfo link

  • @ref: 77588
  • straininfo: 88138

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny12148642Psychrobacter submarinus sp. nov. and Psychrobacter marincola sp. nov., psychrophilic halophiles from marine environments.Romanenko LA, Schumann P, Rohde M, Lysenko AM, Mikhailov VV, Stackebrandt EInt J Syst Evol Microbiol10.1099/00207713-52-4-12912002Animals, Bacterial Typing Techniques, *Cold Temperature, DNA, Ribosomal/analysis, Gammaproteobacteria/chemistry/*classification/genetics/physiology, Genotype, Molecular Sequence Data, Phenotype, Phylogeny, RNA, Ribosomal, 16S/genetics, Seawater/*microbiology, Sequence Analysis, DNA, Sodium Chloride/*metabolism, Urochordata/*microbiologyGenetics
Pathogenicity32438723Structure and in vitro Bioactivity against Cancer Cells of the Capsular Polysaccharide from the Marine Bacterium Psychrobacter marincola.Kokoulin MS, Kuzmich AS, Romanenko LA, Chikalovets IV, Chernikov OVMar Drugs10.3390/md180502682020Animals, Antineoplastic Agents/*pharmacology, HL-60 Cells/*drug effects, Humans, Oceans and Seas, Polysaccharides/*pharmacology, *Psychrobacter, Structure-Activity Relationship

Reference

@idauthorscataloguedoi/urltitle
5236Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 14160)https://www.dsmz.de/collection/catalogue/details/culture/DSM-14160
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
33152Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/5098
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
77588Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID88138.1StrainInfo: A central database for resolving microbial strain identifiers
120803Curators of the CIPCollection of Institut Pasteur (CIP 107640)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20107640