Strain identifier

BacDive ID: 8181

Type strain: Yes

Species: Psychrobacter proteolyticus

Strain Designation: 116

Strain history: CIP <- 2000, E.B.M. Denner, Vienna Biocenter, Inst. Mikrobiol. Gen., Vienna, Austria <- M. Turkiewicz, Inst. Tech. Biochem., Lodz, Poland <- S.P. Donachie: strain 116

NCBI tax ID(s): 147825 (species)

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General

@ref: 5191

BacDive-ID: 8181

DSM-Number: 13887

keywords: 16S sequence, Bacteria, obligate aerobe, psychrophilic, Gram-negative, rod-shaped

description: Psychrobacter proteolyticus 116 is an obligate aerobe, psychrophilic, Gram-negative bacterium that was isolated from stomach specimen of Antarctic krill Euphausia superba.

NCBI tax id

  • NCBI tax id: 147825
  • Matching level: species

strain history

@refhistory
5191<- E. B. M. Denner; 116 <- M. Turkiewicz <- S. P. Donachie
121970CIP <- 2000, E.B.M. Denner, Vienna Biocenter, Inst. Mikrobiol. Gen., Vienna, Austria <- M. Turkiewicz, Inst. Tech. Biochem., Lodz, Poland <- S.P. Donachie: strain 116

doi: 10.13145/bacdive8181.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Pseudomonadales
  • family: Moraxellaceae
  • genus: Psychrobacter
  • species: Psychrobacter proteolyticus
  • full scientific name: Psychrobacter proteolyticus Denner et al. 2001

@ref: 5191

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Pseudomonadales

family: Moraxellaceae

genus: Psychrobacter

species: Psychrobacter proteolyticus

full scientific name: Psychrobacter proteolyticus Denner et al. 2001

strain designation: 116

type strain: yes

Morphology

cell morphology

  • @ref: 121970
  • gram stain: negative
  • cell shape: rod-shaped
  • motility: no

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
5191BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514)yeshttps://mediadive.dsmz.de/medium/514Name: BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514) Composition: NaCl 19.45 g/l MgCl2 5.9 g/l Bacto peptone 5.0 g/l Na2SO4 3.24 g/l CaCl2 1.8 g/l Yeast extract 1.0 g/l KCl 0.55 g/l NaHCO3 0.16 g/l Fe(III) citrate 0.1 g/l KBr 0.08 g/l SrCl2 0.034 g/l H3BO3 0.022 g/l Na2HPO4 0.008 g/l Na-silicate 0.004 g/l NaF 0.0024 g/l (NH4)NO3 0.0016 g/l Distilled water
5191TRYPTONE SOYA BROTH (TSB) (DSMZ Medium 545)yeshttps://mediadive.dsmz.de/medium/545Name: TRYPTONE SOYA BROTH (TSB) (DSMZ Medium 545; with strain-specific modifications) Composition: NaCl 26.0 g/l Casein peptone 17.0 g/l Soy peptone 3.0 g/l D(+)-Glucose 2.5 g/l K2HPO4 2.5 g/l Distilled water
42084MEDIUM 72- for trypto casein soja agaryesDistilled water make up to (1000.000 ml);Trypto casein soy agar (40.000 g)
121970CIP Medium 72yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=72

culture temp

@refgrowthtypetemperaturerange
5191positivegrowth20psychrophilic
5191positivegrowth20-25
42084positivegrowth22psychrophilic
121970positivegrowth5-30
121970nogrowth37mesophilic
121970nogrowth41thermophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 121970
  • oxygen tolerance: obligate aerobe

compound production

  • @ref: 5191
  • compound: metalloprotease

halophily

  • @ref: 121970
  • salt: NaCl
  • growth: positive
  • tested relation: growth
  • concentration: 0-10 %

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
1219704853esculin-hydrolysis
12197017234glucose-fermentation
12197017716lactose-fermentation
12197017632nitrate-reduction
12197016301nitrite-reduction
121970132112sodium thiosulfate+builds gas from
12197017234glucose-degradation
12197017632nitrate-respiration

antibiotic resistance

  • @ref: 121970
  • metabolite: 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)
  • is antibiotic: yes
  • is sensitive: no
  • is resistant: yes

metabolite production

  • @ref: 121970
  • Chebi-ID: 35581
  • metabolite: indole
  • production: no

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testmethylred-test
12197015688acetoin-
12197017234glucose-

enzymes

@refvalueactivityec
121970oxidase+
121970beta-galactosidase-3.2.1.23
121970alcohol dehydrogenase-1.1.1.1
121970amylase-
121970DNase-
121970catalase+1.11.1.6
121970tween esterase+
121970gamma-glutamyltransferase+2.3.2.2
121970lecithinase-
121970lipase+
121970lysine decarboxylase-4.1.1.18
121970ornithine decarboxylase-4.1.1.17
121970tryptophan deaminase-
121970urease+3.5.1.5
68382alkaline phosphatase+3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)+
68382lipase (C 14)-
68382leucine arylamidase+3.4.11.1
68382valine arylamidase-
68382cystine arylamidase-3.4.11.3
68382trypsin-3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase-3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase-3.2.1.20
68382beta-glucosidase-3.2.1.21
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
121970-+++-+----++--------

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
121970--------------------------------------------------+--+--------++---------+-----+++-+----++++++-+++-

Isolation, sampling and environmental information

isolation

@refsample typehost speciesgeographic locationcontinentcountryorigin.countryisolation date
5191stomach specimen of Antarctic krill Euphausia superbaEuphausia superbaSouth Shetlands, King Geaorge Island, Admiralty BayAustralia and Oceania
121970Antarctic krill, stomach specimenSouth Shetland, ScotlandEuropeUnited KingdomGBR1990

isolation source categories

Cat1Cat2Cat3
#Infection#Patient#Specimen
#Host#Arthropoda#Crustacea
#Host Body-Site#Gastrointestinal tract#Stomach

taxonmaps

  • @ref: 69479
  • File name: preview.99_3600.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_80;96_193;97_209;98_234;99_3600&stattab=map
  • Last taxonomy: Psychrobacter
  • 16S sequence: AJ272303
  • Sequence Identity:
  • Total samples: 6652
  • soil counts: 484
  • aquatic counts: 3933
  • animal counts: 2136
  • plant counts: 99

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
51911Risk group (German classification)
1219701Risk group (French classification)

Sequence information

16S sequences

  • @ref: 5191
  • description: Psychrobacter sp. 16S rRNA gene, strain 116
  • accession: AJ272303
  • length: 1426
  • database: ena
  • NCBI tax ID: 147825

External links

@ref: 5191

culture collection no.: DSM 13887, CIP 106830

straininfo link

  • @ref: 77584
  • straininfo: 86022

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny11403398Psychrobacter proteolyticus sp. nov., a psychrotrophic, halotolerant bacterium isolated from the Antarctic krill Euphausia superba Dana, excreting a cold-adapted metalloprotease.Denner EB, Mark B, Busse HJ, Turkiewicz M, Lubitz WSyst Appl Microbiol10.1078/0723-2020-000062001Adaptation, Biological/*physiology, Animals, Antarctic Regions, Bacterial Proteins/isolation & purification, Bacterial Typing Techniques, Cold Temperature, Crustacea/*microbiology, DNA, Ribosomal/genetics, Drug Resistance, Microbial, Fatty Acids/analysis, Gammaproteobacteria/classification/enzymology/*isolation & purification, Gram-Negative Aerobic Rods and Cocci/enzymology/*isolation & purification, Metalloendopeptidases/*metabolism, Molecular Sequence Data, RNA, Ribosomal, 16S/genetics, Salts/pharmacologyEnzymology
Phylogeny15368844Psychrobacter nivimaris sp. nov., a heterotrophic bacterium attached to organic particles isolated from the South Atlantic (Antarctica).Heuchert A, Glockner FO, Amann R, Fischer USyst Appl Microbiol10.1078/07232020414384552004Antarctic Regions, *Bacterial Adhesion, Bacterial Typing Techniques, Base Composition, DNA, Ribosomal, Molecular Sequence Data, Moraxellaceae/*classification/genetics/physiology, Nucleic Acid Hybridization, Organic Chemicals, Particle Size, Phylogeny, RNA, Ribosomal, 16S, Seawater/*microbiology, Sequence Analysis, DNAGenetics

Reference

@idauthorscataloguedoi/urltitle
5191Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 13887)https://www.dsmz.de/collection/catalogue/details/culture/DSM-13887
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
42084Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/19004
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
77584Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID86022.1StrainInfo: A central database for resolving microbial strain identifiers
121970Curators of the CIPCollection of Institut Pasteur (CIP 106830)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20106830