Strain identifier

BacDive ID: 8170

Type strain: Yes

Species: Moraxella cuniculi

Strain Designation: K 19

Strain history: CIP <- 1973, ATCC <- U. Berger: strain K 19, Neisseria cuniculi

NCBI tax ID(s): 1122245 (strain), 34061 (species)

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General

@ref: 15935

BacDive-ID: 8170

DSM-Number: 21768

keywords: genome sequence, 16S sequence, Bacteria, mesophilic

description: Moraxella cuniculi K 19 is a mesophilic bacterium that was isolated from oral mucosa of rabbit.

NCBI tax id

NCBI tax idMatching level
1122245strain
34061species

strain history

@refhistory
15935<- CCUG <- U. Berger, Heidelberg, Germany {1973}
118815CIP <- 1973, ATCC <- U. Berger: strain K 19, Neisseria cuniculi

doi: 10.13145/bacdive8170.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Pseudomonadales
  • family: Moraxellaceae
  • genus: Moraxella
  • species: Moraxella cuniculi
  • full scientific name: Moraxella cuniculi (Berger 1962) Bøvre and Hagen 1984

  • synonyms

    • @ref: 20215
    • synonym: Neisseria cuniculi

@ref: 15935

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Pseudomonadales

family: Moraxellaceae

genus: Moraxella

species: Moraxella cuniculi

full scientific name: Moraxella cuniculi (Berger 1962) Bøvre and Hagen 1984

strain designation: K 19

type strain: yes

Morphology

cell morphology

@refmotilityconfidencegram stain
69480no94.997
6948099.988negative

multimedia

@refmultimedia contentcaptionintellectual property rights
66793EM_DSM_21768_1.jpgelectron microscopic image© HZI/Manfred Rohde
66793EM_DSM_21768_2.jpgelectron microscopic image© HZI/Manfred Rohde
66793EM_DSM_21768_3.jpgelectron microscopic image© HZI/Manfred Rohde
66793EM_DSM_21768_4.jpgelectron microscopic image© HZI/Manfred Rohde
66793EM_DSM_21768_5.jpgelectron microscopic image© HZI/Manfred Rohde

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
15935COLUMBIA BLOOD AGAR (DSMZ Medium 429)yeshttps://mediadive.dsmz.de/medium/429Name: COLUMBIA BLOOD AGAR (DSMZ Medium 429; with strain-specific modifications) Composition: Horse blood 100.0 g/l Columbia agar base
37617MEDIUM 4 - Columbia agar with 20 % sterile defibrinated horse serumyesHorse serum (200.000 ml);Columbia agar (39.000 g);Distilled water make up to (1000.000 ml)
118815CIP Medium 4yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=4

culture temp

@refgrowthtypetemperaturerange
15935positivegrowth37mesophilic
37617positivegrowth30mesophilic

Physiology and metabolism

spore formation

@refspore formationconfidence
69481no100
69480no100

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6837717634D-glucose-builds acid from
6837715824D-fructose-builds acid from
6837717306maltose-builds acid from
6837717992sucrose-builds acid from
6837718257ornithine-degradation
6837727897tryptophan-energy source
6838129016arginine-hydrolysis
6838116988D-ribose-builds acid from
6838116899D-mannitol-builds acid from
6838130911sorbitol-builds acid from
6838117716lactose-builds acid from
6838127082trehalose-builds acid from
6838116634raffinose-builds acid from
6838117992sucrose-builds acid from
6838130849L-arabinose-builds acid from
6838118333D-arabitol-builds acid from
6838140585alpha-cyclodextrin-builds acid from
68381606565hippurate-hydrolysis
6838128087glycogen-builds acid from
6838127941pullulan-builds acid from
6838117306maltose-builds acid from
6838128053melibiose-builds acid from
683816731melezitose-builds acid from
68381320055methyl beta-D-glucopyranoside-builds acid from
6838116443D-tagatose-builds acid from
6838116199urea+hydrolysis

metabolite production

@refChebi-IDmetaboliteproduction
6837735581indoleno
6838115688acetoinno

metabolite tests

@refChebi-IDmetabolitevoges-proskauer-testindole test
6838115688acetoin-
6837735581indole-

enzymes

@refvalueactivityec
68381urease+3.5.1.5
68381beta-mannosidase-3.2.1.25
68381glycyl tryptophan arylamidase-
68381N-acetyl-beta-glucosaminidase-3.2.1.52
68381pyrrolidonyl arylamidase-3.4.19.3
68381beta-galactosidase-3.2.1.23
68381Alanyl-Phenylalanyl-Proline arylamidase-
68381alkaline phosphatase-3.1.3.1
68381alpha-galactosidase-3.2.1.22
68381beta-glucuronidase-3.2.1.31
68381beta-glucosidase-3.2.1.21
68381arginine dihydrolase-3.5.3.6
68377tryptophan deaminase-4.1.99.1
68377gamma-glutamyltransferase-2.3.2.2
68377proline-arylamidase-3.4.11.5
68377beta-galactosidase-3.2.1.23
68377alkaline phosphatase-3.1.3.1
68377ornithine decarboxylase-4.1.1.17
68377beta-lactamase-3.5.2.6

API NH

@refPENGLUFRUMALSACODCURELIPPALbeta GALProAGGTIND
15935------+------
15935-------+-----

API rID32STR

@refADH Argbeta GLUbeta GARbeta GURalpha GALPALRIBMANSORLACTRERAFSACLARADARLCDEXVPAPPAbeta GALPyrAbeta NAGGTAHIPGLYGPULMALMELMLZMbeta DGTAGbeta MANURE
44500-------------------------------+

Isolation, sampling and environmental information

isolation

@refsample typecountryorigin.countrycontinent
15935oral mucosa of rabbitGermanyDEUEurope
44500Rabbit oral mucosa
118815Animal, Rabbit, oral mucosa

isolation source categories

Cat1Cat2Cat3
#Host#Mammals#Leporidae (Rabbit/Hare)
#Host Body-Site#Oral cavity and airways

taxonmaps

  • @ref: 69479
  • File name: preview.99_3028.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_80;96_246;97_1898;98_2330;99_3028&stattab=map
  • Last taxonomy: Moraxella cuniculi subclade
  • 16S sequence: AF005188
  • Sequence Identity:
  • Total samples: 1717
  • soil counts: 25
  • aquatic counts: 87
  • animal counts: 1599
  • plant counts: 6

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
159351Risk group (German classification)
1188151Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218M.cuniculi (NCTC 10297) 16S rRNA geneX79221728ena34061
15935Moraxella cuniculi 16S ribosomal RNA gene, partial sequenceAF0051881446ena34061

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Moraxella cuniculi NCTC10297GCA_900637105completencbi34061
66792Moraxella cuniculi DSM 21768GCA_900156515contigncbi1122245
66792Moraxella cuniculi CCUG 2154GCA_002015175scaffoldncbi34061
66792Moraxella cuniculi DSM 21768 strain DSM 217681122245.3wgspatric1122245
66792Moraxella cuniculi strain CCUG 215434061.3wgspatric34061
66792Moraxella cuniculi strain NCTC1029734061.5completepatric34061
66792Moraxella cuniculi CCUG 21542873786040draftimg34061
66792Moraxella cuniculi DSM 217682681812931draftimg1122245

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno100no
flagellatedno91.027no
gram-positiveno97.744no
anaerobicno98.034no
aerobicno63.08no
halophileno86.807no
spore-formingno97.463no
thermophileno99.367yes
glucose-utilno85.655no
motileno87.495no
glucose-fermentno93.265no

External links

@ref: 15935

culture collection no.: DSM 21768, ATCC 14688, CCUG 2154, LMG 8382, NCTC 10297, ITG 3388, CIP 73.17

straininfo link

  • @ref: 77573
  • straininfo: 3581

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Enzymology11267775Isolation and characterization of type IIS restriction endonuclease from Neisseria cuniculi ATCC 14688.Furmanek B, Gromek K, Sektas M, Kaczorowski TFEMS Microbiol Lett10.1111/j.1574-6968.2001.tb10560.x2001Amino Acid Sequence, Chromatography, Cloning, Molecular, DNA/metabolism, DNA Restriction-Modification Enzymes, Deoxyribonucleases, Type II Site-Specific/genetics/isolation & purification/*metabolism, Isoenzymes/*isolation & purification/metabolism, Molecular Sequence Data, Neisseria/*enzymology, Sequence Analysis, Protein, Substrate SpecificityPhylogeny
Enzymology17306509Molecular characterization of the DNA methyltransferase M1.NcuI from Neisseria cuniculi ATCC 14688.Furmanek B, Sektas M, Wons E, Kaczorowski TRes Microbiol10.1016/j.resmic.2006.10.0062006Amino Acid Sequence, Bacterial Proteins/chemistry/genetics/isolation & purification/metabolism, Base Sequence, Chromatography, *DNA (Cytosine-5-)-Methyltransferases/chemistry/genetics/isolation & purification/metabolism, DNA Restriction Enzymes/genetics, Genes, Bacterial, Methylation, Molecular Sequence Data, Molecular Weight, Neisseria/*enzymology, Sequence AlignmentPhylogeny
Phylogeny19329585Moraxella pluranimalium sp. nov., isolated from animal specimens.Vela AI, Arroyo E, Aragon V, Sanchez-Porro C, Latre MV, Cerda-Cuellar M, Ventosa A, Dominguez L, Fernandez-Garayzabal JFInt J Syst Evol Microbiol10.1099/ijs.0.006205-02009Animals, Bacterial Typing Techniques, Cluster Analysis, DNA, Bacterial/chemistry/genetics, DNA, Ribosomal/chemistry/genetics, Molecular Sequence Data, Moraxella/*classification/genetics/*isolation & purification/physiology, Nucleic Acid Hybridization, Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Sequence Homology, Nucleic Acid, Sheep/*microbiology, Swine/*microbiologyGenetics
Metabolism19332813M1.MboII and M2.MboII type IIS methyltransferases: different specificities, the same target.Furmanek-Blaszk B, Boratynski R, Zolcinska N, Sektas MMicrobiology (Reading)10.1099/mic.0.025023-02009Adenine/analogs & derivatives/*metabolism, Amino Acid Sequence, Animals, Cattle, Cytosine/analogs & derivatives/*metabolism, DNA Methylation, DNA Restriction-Modification Enzymes/chemistry/genetics/metabolism, DNA, Bacterial/chemistry/metabolism, DNA, Single-Stranded/chemistry/metabolism, Molecular Sequence Data, Moraxella bovis/*enzymology, Site-Specific DNA-Methyltransferase (Adenine-Specific)/chemistry/genetics/*metabolism, *Substrate SpecificityEnzymology

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
15935Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 21768)https://www.dsmz.de/collection/catalogue/details/culture/DSM-21768
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
37617Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/10670
44500Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 2154)https://www.ccug.se/strain?id=2154
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
66793Mukherjee et al.10.1038/nbt.3886GEBA: 1,003 reference genomes of bacterial and archaeal isolates expand coverage of the tree of life 35: 676-683 201728604660
66794Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg10.1093/nar/gkaa1025BRENDA, the ELIXIR core data resource in 2021: new developments and updatesNucleic Acids Res. 49: D498-D508 202033211880
68377Automatically annotated from API NH
68381Automatically annotated from API rID32STR
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
77573Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID3581.1StrainInfo: A central database for resolving microbial strain identifiers
118815Curators of the CIPCollection of Institut Pasteur (CIP 73.17)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%2073.17