Strain identifier

BacDive ID: 8169

Type strain: Yes

Species: Moraxella lacunata

Strain history: CIP <- 1953, R. Legroux, Inst. Pasteur, Paris, France: strain Morax 260

NCBI tax ID(s): 477 (species)

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General

@ref: 7271

BacDive-ID: 8169

DSM-Number: 18052

keywords: genome sequence, 16S sequence, Bacteria, obligate aerobe, mesophilic

description: Moraxella lacunata DSM 18052 is an obligate aerobe, mesophilic bacterium that was isolated from human conjunctivitis.

NCBI tax id

  • NCBI tax id: 477
  • Matching level: species

strain history

@refhistory
7271<- CIP <- R. Legronx, Inst. Pasteur Paris; Morax 260
406301953, R. Legroux, Inst. Pasteur, Paris, France: strain Morax 260
67770IAM 13554 <-- NCTC 11011 <-- ATCC 17967 <-- CDC KC784.
120536CIP <- 1953, R. Legroux, Inst. Pasteur, Paris, France: strain Morax 260

doi: 10.13145/bacdive8169.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Pseudomonadales
  • family: Moraxellaceae
  • genus: Moraxella
  • species: Moraxella lacunata
  • full scientific name: Moraxella lacunata (Eyre 1900) Lwoff 1939 (Approved Lists 1980)
  • synonyms

    • @ref: 20215
    • synonym: Bacillus lacunatus

@ref: 7271

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Pseudomonadales

family: Moraxellaceae

genus: Moraxella

species: Moraxella lacunata subsp. lacunata

full scientific name: Moraxella lacunata subsp. lacunata (Eyre 1900) Lwoff 1939

type strain: yes

Morphology

cell morphology

@refmotilityconfidencegram staincell shape
69480no92.262
6948099.996negative
120536nonegativerod-shaped

colony morphology

  • @ref: 7271
  • type of hemolysis: gamma
  • incubation period: 1-2 days

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
7271COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)yeshttps://mediadive.dsmz.de/medium/693Name: COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) Composition: Defibrinated sheep blood 50.0 g/l Columbia agar base
40630MEDIUM 4 - Columbia agar with 20 % sterile defibrinated horse serumyesHorse serum (200.000 ml);Columbia agar (39.000 g);Distilled water make up to (1000.000 ml)
120536CIP Medium 4yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=4

culture temp

@refgrowthtypetemperaturerange
7271positivegrowth30mesophilic
40630positivegrowth30mesophilic
67770positivegrowth37mesophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 120536
  • oxygen tolerance: obligate aerobe

spore formation

@refspore formationconfidence
69481no100
69480no99.993

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
12053617632nitrate+reduction
12053616301nitrite-reduction
6837717634D-glucose-builds acid from
6837715824D-fructose-builds acid from
6837717306maltose-builds acid from
6837717992sucrose-builds acid from
6837718257ornithine-degradation
6837727897tryptophan-energy source

metabolite production

@refChebi-IDmetaboliteproduction
6837735581indoleno
12053635581indoleno

metabolite tests

  • @ref: 68377
  • Chebi-ID: 35581
  • metabolite: indole
  • indole test: -

enzymes

@refvalueactivityec
7271catalase+1.11.1.6
7271cytochrome-c oxidase+1.9.3.1
68377tryptophan deaminase-4.1.99.1
68377beta-galactosidase-3.2.1.23
68377ornithine decarboxylase-4.1.1.17
68377beta-lactamase-3.5.2.6
120536oxidase+
120536catalase+1.11.1.6
120536urease-3.5.1.5
68382alkaline phosphatase+3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)+
68382lipase (C 14)-
68382leucine arylamidase+3.4.11.1
68382valine arylamidase-
68382cystine arylamidase-3.4.11.3
68382trypsin-3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase-3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase+3.2.1.20
68382beta-glucosidase-3.2.1.21
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

fatty acid profile

  • fatty acids

    @reffatty acidpercentageECL
    65621C10:03.110
    65621C12:04.312
    65621C16:07.216
    65621C18:01.318
    65621C12:0 3OH3.413.455
    65621C14:0 3OH/C16:1 ISO I2.615.485
    65621C16:1 ω7c29.915.819
    65621C16:1 ω9c1.415.774
    65621C18:1 ω9c39.117.769
    65621C18:2 ω6,9c/C18:0 ANTE4.417.724
    65621C18:3 ω6,9,12c3.417.577
  • type of FA analysis: whole cell analysis
  • method/protocol: CCUG

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
120536-+++-+----++---+----

API NH

@refPENGLUFRUMALSACODCURELIPPALbeta GALProAGGTIND
7271------+/-+/---+/---
7271-------+/-+/---+/--

Isolation, sampling and environmental information

isolation

@refsample typecountryorigin.countrycontinent
7271human conjunctivitisFranceFRAEurope
67770Conjunctivitis
120536Human, Conjunctivitis

isolation source categories

Cat1Cat2Cat3
#Infection#Inflammation
#Host#Human
#Host Body-Site#Organ#Eye

taxonmaps

  • @ref: 69479
  • File name: preview.99_2385.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_80;96_246;97_671;98_1857;99_2385&stattab=map
  • Last taxonomy: Moraxella lacunata
  • 16S sequence: AB681721
  • Sequence Identity:
  • Total samples: 720
  • soil counts: 2
  • aquatic counts: 36
  • animal counts: 682

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
72712Risk group (German classification)
1205361Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Moraxella lacunata 16S rRNA gene, partialAJ247227446ena477
20218Moraxella lacunata gene for 16S ribosomal RNAD640491519ena477
20218M.lacunata gene for 16S rRNAX74895729ena477
7271Moraxella lacunata 16S ribosomal RNA gene, partial sequenceAF0051601446ena477
67770Moraxella lacunata gene for 16S rRNA, partial sequence, strain: NBRC 102154AB6817211455ena477

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Moraxella lacunata NBRC 1021541223506.3wgspatric1223506
66792Moraxella lacunata strain CCUG 4441477.6wgspatric477
66792Moraxella lacunata CCUG 44412902269242draftimg477
66792Moraxella lacunata NBRC 1021542731957642draftimg1223506
67770Moraxella lacunata NBRC 102154GCA_001591245contigncbi1223506
67770Moraxella lacunata CCUG 4441GCA_002027545scaffoldncbi477

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno100no
motileno88.729no
gram-positiveno97.34no
anaerobicno97.698no
halophileno79.59no
spore-formingno96.438no
thermophileno99.545no
glucose-utilno79.186no
flagellatedno90.362no
aerobicno83.504no
glucose-fermentno92.363no

External links

@ref: 7271

culture collection no.: DSM 18052, ATCC 17967, CCUG 4441, CIP A182, NCTC 11011, Morax 260, JCM 20914, CDC KC784, IAM 12150, IAM 13554, LMG 5301, NBRC 102154

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
7271Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 18052)https://www.dsmz.de/collection/catalogue/details/culture/DSM-18052
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
40630Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/10396
65621Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 4441)https://www.ccug.se/strain?id=4441
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
67770Curators of the JCMhttps://jcm.brc.riken.jp/en/
68377Automatically annotated from API NH
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
120536Curators of the CIPCollection of Institut Pasteur (CIP A182)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20A182