Strain identifier
BacDive ID: 8169
Type strain:
Species: Moraxella lacunata
Strain history: CIP <- 1953, R. Legroux, Inst. Pasteur, Paris, France: strain Morax 260
NCBI tax ID(s): 477 (species)
General
@ref: 7271
BacDive-ID: 8169
DSM-Number: 18052
keywords: genome sequence, 16S sequence, Bacteria, obligate aerobe, mesophilic
description: Moraxella lacunata DSM 18052 is an obligate aerobe, mesophilic bacterium that was isolated from human conjunctivitis.
NCBI tax id
- NCBI tax id: 477
- Matching level: species
strain history
@ref | history |
---|---|
7271 | <- CIP <- R. Legronx, Inst. Pasteur Paris; Morax 260 |
40630 | 1953, R. Legroux, Inst. Pasteur, Paris, France: strain Morax 260 |
67770 | IAM 13554 <-- NCTC 11011 <-- ATCC 17967 <-- CDC KC784. |
120536 | CIP <- 1953, R. Legroux, Inst. Pasteur, Paris, France: strain Morax 260 |
doi: 10.13145/bacdive8169.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/pseudomonadota
- domain: Bacteria
- phylum: Pseudomonadota
- class: Gammaproteobacteria
- order: Pseudomonadales
- family: Moraxellaceae
- genus: Moraxella
- species: Moraxella lacunata
- full scientific name: Moraxella lacunata (Eyre 1900) Lwoff 1939 (Approved Lists 1980)
synonyms
- @ref: 20215
- synonym: Bacillus lacunatus
@ref: 7271
domain: Bacteria
phylum: Proteobacteria
class: Gammaproteobacteria
order: Pseudomonadales
family: Moraxellaceae
genus: Moraxella
species: Moraxella lacunata subsp. lacunata
full scientific name: Moraxella lacunata subsp. lacunata (Eyre 1900) Lwoff 1939
type strain: yes
Morphology
cell morphology
@ref | motility | confidence | gram stain | cell shape |
---|---|---|---|---|
69480 | no | 92.262 | ||
69480 | 99.996 | negative | ||
120536 | no | negative | rod-shaped |
colony morphology
- @ref: 7271
- type of hemolysis: gamma
- incubation period: 1-2 days
Culture and growth conditions
culture medium
@ref | name | growth | link | composition |
---|---|---|---|---|
7271 | COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) | yes | https://mediadive.dsmz.de/medium/693 | Name: COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) Composition: Defibrinated sheep blood 50.0 g/l Columbia agar base |
40630 | MEDIUM 4 - Columbia agar with 20 % sterile defibrinated horse serum | yes | Horse serum (200.000 ml);Columbia agar (39.000 g);Distilled water make up to (1000.000 ml) | |
120536 | CIP Medium 4 | yes | https://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=4 |
culture temp
@ref | growth | type | temperature | range |
---|---|---|---|---|
7271 | positive | growth | 30 | mesophilic |
40630 | positive | growth | 30 | mesophilic |
67770 | positive | growth | 37 | mesophilic |
Physiology and metabolism
oxygen tolerance
- @ref: 120536
- oxygen tolerance: obligate aerobe
spore formation
@ref | spore formation | confidence |
---|---|---|
69481 | no | 100 |
69480 | no | 99.993 |
metabolite utilization
@ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
---|---|---|---|---|
120536 | 17632 | nitrate | + | reduction |
120536 | 16301 | nitrite | - | reduction |
68377 | 17634 | D-glucose | - | builds acid from |
68377 | 15824 | D-fructose | - | builds acid from |
68377 | 17306 | maltose | - | builds acid from |
68377 | 17992 | sucrose | - | builds acid from |
68377 | 18257 | ornithine | - | degradation |
68377 | 27897 | tryptophan | - | energy source |
metabolite production
@ref | Chebi-ID | metabolite | production |
---|---|---|---|
68377 | 35581 | indole | no |
120536 | 35581 | indole | no |
metabolite tests
- @ref: 68377
- Chebi-ID: 35581
- metabolite: indole
- indole test: -
enzymes
@ref | value | activity | ec |
---|---|---|---|
7271 | catalase | + | 1.11.1.6 |
7271 | cytochrome-c oxidase | + | 1.9.3.1 |
68377 | tryptophan deaminase | - | 4.1.99.1 |
68377 | beta-galactosidase | - | 3.2.1.23 |
68377 | ornithine decarboxylase | - | 4.1.1.17 |
68377 | beta-lactamase | - | 3.5.2.6 |
120536 | oxidase | + | |
120536 | catalase | + | 1.11.1.6 |
120536 | urease | - | 3.5.1.5 |
68382 | alkaline phosphatase | + | 3.1.3.1 |
68382 | esterase (C 4) | + | |
68382 | esterase lipase (C 8) | + | |
68382 | lipase (C 14) | - | |
68382 | leucine arylamidase | + | 3.4.11.1 |
68382 | valine arylamidase | - | |
68382 | cystine arylamidase | - | 3.4.11.3 |
68382 | trypsin | - | 3.4.21.4 |
68382 | alpha-chymotrypsin | - | 3.4.21.1 |
68382 | acid phosphatase | + | 3.1.3.2 |
68382 | naphthol-AS-BI-phosphohydrolase | + | |
68382 | alpha-galactosidase | - | 3.2.1.22 |
68382 | beta-galactosidase | - | 3.2.1.23 |
68382 | beta-glucuronidase | - | 3.2.1.31 |
68382 | alpha-glucosidase | + | 3.2.1.20 |
68382 | beta-glucosidase | - | 3.2.1.21 |
68382 | N-acetyl-beta-glucosaminidase | - | 3.2.1.52 |
68382 | alpha-mannosidase | - | 3.2.1.24 |
68382 | alpha-fucosidase | - | 3.2.1.51 |
fatty acid profile
fatty acids
@ref fatty acid percentage ECL 65621 C10:0 3.1 10 65621 C12:0 4.3 12 65621 C16:0 7.2 16 65621 C18:0 1.3 18 65621 C12:0 3OH 3.4 13.455 65621 C14:0 3OH/C16:1 ISO I 2.6 15.485 65621 C16:1 ω7c 29.9 15.819 65621 C16:1 ω9c 1.4 15.774 65621 C18:1 ω9c 39.1 17.769 65621 C18:2 ω6,9c/C18:0 ANTE 4.4 17.724 65621 C18:3 ω6,9,12c 3.4 17.577 - type of FA analysis: whole cell analysis
- method/protocol: CCUG
API zym
@ref | Control | Alkaline phosphatase | Esterase | Esterase Lipase | Lipase | Leucine arylamidase | Valine arylamidase | Cystine arylamidase | Trypsin | alpha- Chymotrypsin | Acid phosphatase | Naphthol-AS-BI-phosphohydrolase | alpha- Galactosidase | beta- Galactosidase | beta- Glucuronidase | alpha- Glucosidase | beta- Glucosidase | N-acetyl-beta- glucosaminidase | alpha- Mannosidase | alpha- Fucosidase |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
120536 | - | + | + | + | - | + | - | - | - | - | + | + | - | - | - | + | - | - | - | - |
API NH
@ref | PEN | GLU | FRU | MAL | SAC | ODC | URE | LIP | PAL | beta GAL | ProA | GGT | IND |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
7271 | - | - | - | - | - | - | +/- | +/- | - | - | +/- | - | - |
7271 | - | - | - | - | - | - | - | +/- | +/- | - | - | +/- | - |
Isolation, sampling and environmental information
isolation
@ref | sample type | country | origin.country | continent |
---|---|---|---|---|
7271 | human conjunctivitis | France | FRA | Europe |
67770 | Conjunctivitis | |||
120536 | Human, Conjunctivitis |
isolation source categories
Cat1 | Cat2 | Cat3 |
---|---|---|
#Infection | #Inflammation | |
#Host | #Human | |
#Host Body-Site | #Organ | #Eye |
taxonmaps
- @ref: 69479
- File name: preview.99_2385.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_80;96_246;97_671;98_1857;99_2385&stattab=map
- Last taxonomy: Moraxella lacunata
- 16S sequence: AB681721
- Sequence Identity:
- Total samples: 720
- soil counts: 2
- aquatic counts: 36
- animal counts: 682
Safety information
risk assessment
@ref | biosafety level | biosafety level comment |
---|---|---|
7271 | 2 | Risk group (German classification) |
120536 | 1 | Risk group (French classification) |
Sequence information
16S sequences
@ref | description | accession | length | database | NCBI tax ID |
---|---|---|---|---|---|
20218 | Moraxella lacunata 16S rRNA gene, partial | AJ247227 | 446 | ena | 477 |
20218 | Moraxella lacunata gene for 16S ribosomal RNA | D64049 | 1519 | ena | 477 |
20218 | M.lacunata gene for 16S rRNA | X74895 | 729 | ena | 477 |
7271 | Moraxella lacunata 16S ribosomal RNA gene, partial sequence | AF005160 | 1446 | ena | 477 |
67770 | Moraxella lacunata gene for 16S rRNA, partial sequence, strain: NBRC 102154 | AB681721 | 1455 | ena | 477 |
Genome sequences
@ref | description | accession | assembly level | database | NCBI tax ID |
---|---|---|---|---|---|
66792 | Moraxella lacunata NBRC 102154 | 1223506.3 | wgs | patric | 1223506 |
66792 | Moraxella lacunata strain CCUG 4441 | 477.6 | wgs | patric | 477 |
66792 | Moraxella lacunata CCUG 4441 | 2902269242 | draft | img | 477 |
66792 | Moraxella lacunata NBRC 102154 | 2731957642 | draft | img | 1223506 |
67770 | Moraxella lacunata NBRC 102154 | GCA_001591245 | contig | ncbi | 1223506 |
67770 | Moraxella lacunata CCUG 4441 | GCA_002027545 | scaffold | ncbi | 477 |
Genome-based predictions
predictions
trait | prediction | confidence | training_data |
---|---|---|---|
spore-forming | no | 100 | no |
motile | no | 88.729 | no |
gram-positive | no | 97.34 | no |
anaerobic | no | 97.698 | no |
halophile | no | 79.59 | no |
spore-forming | no | 96.438 | no |
thermophile | no | 99.545 | no |
glucose-util | no | 79.186 | no |
flagellated | no | 90.362 | no |
aerobic | no | 83.504 | no |
glucose-ferment | no | 92.363 | no |
External links
@ref: 7271
culture collection no.: DSM 18052, ATCC 17967, CCUG 4441, CIP A182, NCTC 11011, Morax 260, JCM 20914, CDC KC784, IAM 12150, IAM 13554, LMG 5301, NBRC 102154
Reference
@id | authors | catalogue | doi/url | title | journal | pubmed |
---|---|---|---|---|---|---|
7271 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 18052) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-18052 | |||
20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |||
20218 | Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P. | 10.1016/j.syapm.2013.11.002 | StrainInfo introduces electronic passports for microorganisms. | Syst Appl Microbiol. 37: 42-50 2014 | 24321274 | |
40630 | Curators of the CIP | https://brclims.pasteur.fr/brcWeb/souche/detail/1/10396 | ||||
65621 | Curators of the CCUG | Culture Collection University of Gothenburg (CCUG) (CCUG 4441) | https://www.ccug.se/strain?id=4441 | |||
66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |||
67770 | Curators of the JCM | https://jcm.brc.riken.jp/en/ | ||||
68377 | Automatically annotated from API NH | |||||
68382 | Automatically annotated from API zym | |||||
69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | https://microbeatlas.org/ | MicrobeAtlas 1.0 beta | |||
69480 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |||
69481 | Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch | 10.21203/rs.3.rs-2527258/v1 | Predictions based on the model GenomeNet Sporulation v. 1 | |||
120536 | Curators of the CIP | Collection of Institut Pasteur (CIP A182) | https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20A182 |