Strain identifier
BacDive ID: 8161
Type strain:
Species: Moraxella catarrhalis
Strain Designation: Ne 11, Nell
Strain history: CIP <- 1973, ATCC <- B.W. Catlin: strain Ne 11 <- Rochester Univ.
NCBI tax ID(s): 480 (species)
General
@ref: 3484
BacDive-ID: 8161
DSM-Number: 9143
keywords: genome sequence, 16S sequence, Bacteria, obligate aerobe, mesophilic, Gram-negative, coccus-shaped, human pathogen
description: Moraxella catarrhalis Ne 11 is an obligate aerobe, mesophilic, Gram-negative human pathogen of the family Moraxellaceae.
NCBI tax id
- NCBI tax id: 480
- Matching level: species
strain history
@ref | history |
---|---|
3484 | <- ATCC; ATCC 25238 <- B. W. Catlin; <- University of Rochester, New York; Nell |
117212 | CIP <- 1973, ATCC <- B.W. Catlin: strain Ne 11 <- Rochester Univ. |
doi: 10.13145/bacdive8161.20230509.8.1
Name and taxonomic classification
LPSN
- @ref: 20215
- description: domain/bacteria
- keyword: phylum/pseudomonadota
- domain: Bacteria
- phylum: Pseudomonadota
- class: Gammaproteobacteria
- order: Pseudomonadales
- family: Moraxellaceae
- genus: Moraxella
- species: Moraxella catarrhalis
- full scientific name: Moraxella catarrhalis (Frosch and Kolle 1896) Henriksen and Bøvre 1968 (Approved Lists 1980)
synonyms
@ref synonym 20215 Branhamella catarrhalis 20215 Mikrokokkus catarrhalis
@ref: 3484
domain: Bacteria
phylum: Proteobacteria
class: Gammaproteobacteria
order: Pseudomonadales
family: Moraxellaceae
genus: Moraxella
species: Moraxella catarrhalis
full scientific name: Moraxella catarrhalis (Frosch and Kolle 1896) Henriksen and Bøvre 1968
strain designation: Ne 11, Nell
type strain: yes
Morphology
cell morphology
- @ref: 117212
- gram stain: negative
- cell shape: coccus-shaped
- motility: no
colony morphology
@ref | type of hemolysis | incubation period |
---|---|---|
3484 | gamma | 1-2 days |
117212 |
Culture and growth conditions
culture medium
@ref | name | growth | link | composition |
---|---|---|---|---|
3484 | COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) | yes | https://mediadive.dsmz.de/medium/693 | Name: COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) Composition: Defibrinated sheep blood 50.0 g/l Columbia agar base |
3484 | BHI MEDIUM (DSMZ Medium 215) | yes | https://mediadive.dsmz.de/medium/215 | Name: BHI MEDIUM (DSMZ Medium 215) Composition: Brain heart infusion 37.0 g/l Distilled water |
37341 | MEDIUM 72- for trypto casein soja agar | yes | Distilled water make up to (1000.000 ml);Trypto casein soy agar (40.000 g) | |
117212 | CIP Medium 72 | yes | https://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=72 |
culture temp
@ref | growth | type | temperature | range |
---|---|---|---|---|
3484 | positive | growth | 37 | mesophilic |
37341 | positive | growth | 30 | mesophilic |
117212 | positive | growth | 30-37 | mesophilic |
117212 | no | growth | 25 | mesophilic |
117212 | no | growth | 41 | thermophilic |
Physiology and metabolism
oxygen tolerance
- @ref: 117212
- oxygen tolerance: obligate aerobe
metabolite utilization
@ref | Chebi-ID | metabolite | utilization activity | kind of utilization tested |
---|---|---|---|---|
117212 | 4853 | esculin | - | hydrolysis |
117212 | 606565 | hippurate | + | hydrolysis |
117212 | 17632 | nitrate | - | builds gas from |
117212 | 17632 | nitrate | + | reduction |
117212 | 16301 | nitrite | - | builds gas from |
117212 | 16301 | nitrite | - | reduction |
117212 | 17632 | nitrate | - | respiration |
68377 | 17634 | D-glucose | - | builds acid from |
68377 | 15824 | D-fructose | - | builds acid from |
68377 | 17306 | maltose | - | builds acid from |
68377 | 17992 | sucrose | - | builds acid from |
68377 | 18257 | ornithine | - | degradation |
68377 | 27897 | tryptophan | - | energy source |
metabolite production
@ref | Chebi-ID | metabolite | production |
---|---|---|---|
68377 | 35581 | indole | no |
117212 | 35581 | indole | no |
117212 | polysaccharides | no |
metabolite tests
- @ref: 68377
- Chebi-ID: 35581
- metabolite: indole
- indole test: -
enzymes
@ref | value | activity | ec |
---|---|---|---|
3484 | catalase | + | 1.11.1.6 |
3484 | cytochrome-c oxidase | + | 1.9.3.1 |
68377 | tryptophan deaminase | - | 4.1.99.1 |
68377 | proline-arylamidase | - | 3.4.11.5 |
68377 | beta-galactosidase | - | 3.2.1.23 |
68377 | ornithine decarboxylase | - | 4.1.1.17 |
68377 | beta-lactamase | - | 3.5.2.6 |
117212 | oxidase | + | |
117212 | alcohol dehydrogenase | - | 1.1.1.1 |
117212 | gelatinase | - | |
117212 | amylase | - | |
117212 | DNase | + | |
117212 | catalase | + | 1.11.1.6 |
117212 | tween esterase | - | |
117212 | gamma-glutamyltransferase | - | 2.3.2.2 |
117212 | lysine decarboxylase | - | 4.1.1.18 |
117212 | ornithine decarboxylase | - | 4.1.1.17 |
117212 | urease | - | 3.5.1.5 |
68382 | alkaline phosphatase | + | 3.1.3.1 |
68382 | esterase (C 4) | + | |
68382 | esterase lipase (C 8) | + | |
68382 | lipase (C 14) | + | |
68382 | leucine arylamidase | + | 3.4.11.1 |
68382 | valine arylamidase | + | |
68382 | cystine arylamidase | - | 3.4.11.3 |
68382 | trypsin | - | 3.4.21.4 |
68382 | alpha-chymotrypsin | - | 3.4.21.1 |
68382 | acid phosphatase | + | 3.1.3.2 |
68382 | naphthol-AS-BI-phosphohydrolase | + | |
68382 | alpha-galactosidase | - | 3.2.1.22 |
68382 | beta-galactosidase | - | 3.2.1.23 |
68382 | beta-glucuronidase | - | 3.2.1.31 |
68382 | alpha-glucosidase | - | 3.2.1.20 |
68382 | beta-glucosidase | - | 3.2.1.21 |
68382 | N-acetyl-beta-glucosaminidase | - | 3.2.1.52 |
68382 | alpha-mannosidase | - | 3.2.1.24 |
68382 | alpha-fucosidase | - | 3.2.1.51 |
fatty acid profile
fatty acids
@ref fatty acid percentage ECL 44172 C10:0 15.8 10 44172 C12:0 8.8 12 44172 C16:0 4.8 16 44172 C12:0 3OH 14 13.455 44172 C16:1 ω7c 9.5 15.819 44172 C17:1 ω8c 2.9 16.792 44172 C18:1 ω9c 26.4 17.769 44172 C18:2 ω6,9c/C18:0 ANTE 11.7 17.724 44172 Unidentified 2.6 10.241 44172 Unidentified 3.6 18.095 - type of FA analysis: whole cell analysis
- method/protocol: CCUG
API zym
@ref | Control | Alkaline phosphatase | Esterase | Esterase Lipase | Lipase | Leucine arylamidase | Valine arylamidase | Cystine arylamidase | Trypsin | alpha- Chymotrypsin | Acid phosphatase | Naphthol-AS-BI-phosphohydrolase | alpha- Galactosidase | beta- Galactosidase | beta- Glucuronidase | alpha- Glucosidase | beta- Glucosidase | N-acetyl-beta- glucosaminidase | alpha- Mannosidase | alpha- Fucosidase |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
117212 | - | + | + | + | + | + | + | - | - | - | + | + | - | - | - | - | - | - | - | - |
API NH
@ref | PEN | GLU | FRU | MAL | SAC | ODC | URE | LIP | PAL | beta GAL | ProA | GGT | IND |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
3484 | - | - | - | - | - | - | - | + | - | - | - | - | - |
44172 | - | - | - | - | - | - | - | + | - | - | - | - | - |
3484 | - | - | - | - | - | - | + | - | - | - | - | - | - |
3484 | - | - | - | - | - | - | + | - | - | - | - | - | - |
3484 | - | - | - | - | - | - | - | + | + | - | - | - | - |
3484 | - | - | - | - | - | - | - | + | - | - | - | - | - |
3484 | - | - | - | - | - | - | - | + | - | - | - | - | - |
3484 | - | - | - | - | - | - | - | + | +/- | - | - | + | - |
API biotype100
@ref | GLU | FRU | GAL | TRE | MNE | SBE | MEL | SAC | RAF | MTE | MAL | LAC | LTE | MbGa | MaGa | CEL | GEN | MbGu | ESC | RIB | ARA | XYL | PLE | RHA | FUC | MLZ | DARL | LARL | XLT | DUL | TAG | GLY | INO | MAN | MTL | TUR | SOR | ADO | HBG | LYX | ERY | MDG | 3MDG | SAT | MUC | LTAT | DTAT | MTAT | DMLT | LMLT | CATE | TATE | TTE | CIT | GRT | GAT | 2KG | 5KG | TRY | NAG | GNT | PAC | PAT | pOBE | QAT | GTE | mOBE | BAT | PPAT | CMT | TGE | BET | PCE | ABT | HIN | LAT | CAP | CYT | HIS | SUC | FUM | GRE | GYT | AVT | ETN | TTN | GLN | ITA | 3OBU | APT | GTT | PRO | DALA | LALA | SER | MNT | PROP | TYR | 2KT |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
117212 | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - | - |
Isolation, sampling and environmental information
isolation
- @ref: 3484
- geographic location: NY, Rochester
- country: USA
- origin.country: USA
- continent: North America
taxonmaps
- @ref: 69479
- File name: preview.99_650.png
- url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_80;96_246;97_268;98_298;99_650&stattab=map
- Last taxonomy: Moraxella
- 16S sequence: U10876
- Sequence Identity:
- Total samples: 27444
- soil counts: 986
- aquatic counts: 3037
- animal counts: 23016
- plant counts: 405
Safety information
risk assessment
@ref | pathogenicity human | pathogenicity animal | biosafety level | biosafety level comment |
---|---|---|---|---|
3484 | yes | yes | 2 | Risk group (German classification) |
117212 | 1 | Risk group (French classification) |
Sequence information
16S sequences
@ref | description | accession | length | database | NCBI tax ID |
---|---|---|---|---|---|
20218 | Moraxella catarrhalis 16S rRNA gene, partial | AJ247218 | 447 | ena | 480 |
20218 | Moraxella catarrhalis ATCC 25238 16S rRNA gene | U10876 | 1511 | ena | 480 |
3484 | Moraxella catarrhalis 16S ribosomal RNA gene, partial sequence | AF005185 | 1446 | ena | 480 |
Genome sequences
@ref | description | accession | assembly level | database | NCBI tax ID |
---|---|---|---|---|---|
66792 | Moraxella catarrhalis NCTC11020 | GCA_900476075 | complete | ncbi | 480 |
66792 | Moraxella catarrhalis CCUG 353 | GCA_001632285 | scaffold | ncbi | 480 |
66792 | Moraxella catarrhalis strain CCUG 353 | 480.224 | wgs | patric | 480 |
66792 | Moraxella catarrhalis strain NCTC11020 | 480.283 | complete | patric | 480 |
66792 | Moraxella catarrhalis CCUG 353 | 2744054833 | draft | img | 480 |
66792 | Moraxella catarrhalis NCTC 11020 | 2847058841 | complete | img | 480 |
Genome-based predictions
predictions
trait | prediction | confidence | training_data |
---|---|---|---|
flagellated | no | 94.762 | no |
gram-positive | no | 97.664 | no |
anaerobic | no | 99.356 | no |
aerobic | no | 85.334 | no |
halophile | no | 78.328 | no |
spore-forming | no | 97.466 | no |
thermophile | no | 98.189 | yes |
glucose-util | no | 77.549 | no |
motile | no | 92.41 | no |
glucose-ferment | no | 93.16 | no |
External links
@ref: 3484
culture collection no.: CCUG 353, NCTC 11020, LMG 11192, DSM 9143, ATCC 25238, CIP 73.21
straininfo link
- @ref: 77565
- straininfo: 3590
literature
topic | Pubmed-ID | title | authors | journal | DOI | year | mesh | topic2 |
---|---|---|---|---|---|---|---|---|
Enzymology | 234507 | Enzyme electrophoretograms in the analysis of taxon relatedness of Micrococcus cryophilus, Branhamella catarrhalis and atypical Neisserias. | Fox RH, McClain DE | J Gen Microbiol | 10.1099/00221287-86-2-210 | 1975 | Acetates/metabolism, Butyrates/metabolism, Cell-Free System, Electrophoresis, Polyacrylamide Gel, Esterases/metabolism, Isocitrate Dehydrogenase/metabolism, Isoenzymes/metabolism, Malate Dehydrogenase/metabolism, Micrococcus/*classification/enzymology, NADP/metabolism, Neisseria/*classification/enzymology, Neisseriaceae/*classification/enzymology, Propionates/metabolism, Species Specificity, Spectrophotometry | Metabolism |
Enzymology | 1592697 | Permeability of the outer membrane of Moraxella catarrhalis for beta-lactam antibiotics. | Gotoh N, Tanaka S, Nishino T | J Antimicrob Chemother | 10.1093/jac/29.3.279 | 1992 | Anti-Bacterial Agents/*pharmacokinetics, Cell Membrane/metabolism, Cell Membrane Permeability, Diffusion, Kinetics, Liposomes/metabolism, Microbial Sensitivity Tests, Moraxella catarrhalis/*metabolism/ultrastructure, beta-Lactams | Metabolism |
Enzymology | 2514110 | Esterase electrophoresis: a molecular tool for studying the epidemiology of Branhamella catarrhalis nosocomial infection. | Picard B, Goullet P, Denamur E, Suermondt G | Epidemiol Infect | 10.1017/s0950268800030946 | 1989 | Adult, Bacterial Infections/epidemiology/*microbiology, Child, Cross Infection/epidemiology/*microbiology, *Disease Outbreaks, Electrophoresis, Esterases/*analysis, Humans, Infant, Newborn, Moraxella catarrhalis/*classification/enzymology, Respiratory Tract Infections/epidemiology/microbiology | Pathogenicity |
Enzymology | 7516823 | Structural studies of the O-polysaccharide from the lipopolysaccharide of Moraxella (Branhamella) catarrhalis serotype A (strain ATCC 25238). | Edebrink P, Jansson PE, Rahman MM, Widmalm G, Holme T, Rahman M, Weintraub A | Carbohydr Res | 10.1016/0008-6215(94)80040-5 | 1994 | Carbohydrate Conformation, Carbohydrate Sequence, Electrophoresis, Polyacrylamide Gel, Glucosamine/analysis, Indicators and Reagents, Magnetic Resonance Spectroscopy, Mass Spectrometry, Methylation, Molecular Sequence Data, Moraxella catarrhalis/*chemistry/classification/immunology, O Antigens, Oligosaccharides/chemistry/isolation & purification, Polysaccharides, Bacterial/*chemistry/isolation & purification, Serotyping, Sugar Acids/analysis | Phylogeny |
Enzymology | 7898388 | Restriction fragment length polymorphism (RFLP) of genomic DNA of Moraxella (Branhamella) catarrhalis isolates in a hospital. | Kawakami Y, Ueno I, Katsuyama T, Furihata K, Matsumoto H | Microbiol Immunol | 10.1111/j.1348-0421.1994.tb02142.x | 1994 | Electrophoresis, Gel, Pulsed-Field, Genome, Bacterial, Humans, Molecular Epidemiology/methods, Moraxella catarrhalis/*classification, Neisseriaceae Infections/microbiology, *Polymorphism, Restriction Fragment Length | Genetics |
Genetics | 8119289 | Characterization of the lipopolysaccharide of Moraxella catarrhalis. Structural analysis of the lipid A from M. catarrhalis serotype A lipopolysaccharide. | Masoud H, Perry MB, Richards JC | Eur J Biochem | 10.1111/j.1432-1033.1994.tb18616.x | 1994 | Carbohydrate Sequence, Fatty Acids/analysis, Hydroxylation, Lipid A/*chemistry, Magnetic Resonance Spectroscopy, Molecular Sequence Data, Molecular Structure, Moraxella catarrhalis/*chemistry/classification, Phosphates/chemistry, Serotyping, Spectrometry, Mass, Fast Atom Bombardment | Phylogeny |
Genetics | 8577264 | Construction of a combined NotI/SmaI physical and genetic map of Moraxella (Branhamella) catarrhalis strain ATCC25238. | Furihata K, Sato K, Matsumoto H | Microbiol Immunol | 10.1111/j.1348-0421.1995.tb03266.x | 1995 | Amino Acid Sequence, Base Composition, Cloning, Molecular, DNA, Bacterial/isolation & purification, *Deoxyribonucleases, Type II Site-Specific, Gene Library, Genes, Bacterial, *Genome, Bacterial, Humans, Molecular Sequence Data, Molecular Weight, Moraxella catarrhalis/*genetics, *Restriction Mapping | Enzymology |
9573066 | Synthesis and characterization of lipooligosaccharide-based conjugates as vaccine candidates for Moraxella (Branhamella) catarrhalis. | Gu XX, Chen J, Barenkamp SJ, Robbins JB, Tsai CM, Lim DJ, Battey J | Infect Immun | 10.1128/IAI.66.5.1891-1897.1998 | 1998 | Animals, Antibodies, Bacterial/blood, Bacterial Vaccines/*immunology, Female, Immune Sera/immunology, Lipopolysaccharides/*immunology, Mice, Moraxella catarrhalis/*immunology, Rabbits, Tetanus Toxoid/immunology, Vaccines, Conjugate/immunology, Vaccines, Synthetic/*immunology | ||
Genetics | 10383971 | Characterization of the Moraxella catarrhalis uspA1 and uspA2 genes and their encoded products. | Cope LD, Lafontaine ER, Slaughter CA, Hasemann CA Jr, Aebi C, Henderson FW, McCracken GH Jr, Hansen EJ | J Bacteriol | 10.1128/JB.181.13.4026-4034.1999 | 1999 | Amino Acid Sequence, Antigens, Bacterial/biosynthesis/*genetics, Antigens, Surface/biosynthesis/*genetics, Bacterial Outer Membrane Proteins/biosynthesis/*genetics, Base Sequence, Gene Expression, *Genes, Bacterial, Mass Spectrometry, Molecular Sequence Data, Molecular Weight, Moraxella catarrhalis/*genetics, Protein Biosynthesis, Repetitive Sequences, Amino Acid, Sequence Analysis, Sequence Homology, Amino Acid, Species Specificity, Transcription, Genetic | Transcriptome |
Enzymology | 10420581 | Construction of a genomic map of Moraxella (Branhamella) catarrhalis ATCC 25238 and physical mapping of virulence-associated genes. | Nguyen KT, Hansen EJ, Farinha MA | Can J Microbiol | 1999 | Blotting, Southern, *Chromosome Mapping, Deoxyribonucleases, Type II Site-Specific/metabolism, Electrophoresis, Gel, Pulsed-Field, *Genes, Bacterial, Moraxella catarrhalis/*genetics/*pathogenicity, Oligonucleotide Probes, RNA, Ribosomal, 23S/genetics, Restriction Mapping, Virulence/genetics, rRNA Operon | Metabolism | |
Pathogenicity | 22703292 | Antimicrobial and antioxidant properties of Betula aetnensis Rafin. (Betulaceae) leaves extract. | Acquaviva R, Menichini F, Ragusa S, Genovese C, Amodeo A, Tundis R, Loizzo MR, Iauk L | Nat Prod Res | 10.1080/14786419.2012.695364 | 2012 | Anti-Bacterial Agents/*chemistry/pharmacology, Antioxidants/*chemistry, Betula/*chemistry, Haemophilus influenzae/drug effects, Microbial Sensitivity Tests, Moraxella catarrhalis/drug effects, Plant Extracts/*chemistry/pharmacology, Plant Leaves/*chemistry | |
Genetics | 27313296 | Draft Genome Sequence of Moraxella catarrhalis Type Strain CCUG 353T. | Jakobsson HE, Salva-Serra F, Thorell K, Gonzales-Siles L, Boulund F, Karlsson R, Sikora P, Engstrand L, Kristiansson E, Moore ER | Genome Announc | 10.1128/genomeA.00552-16 | 2016 | ||
30464556 | Novel mechanism responsible for high-level macrolide resistance in Moraxella catarrhalis. | Kasai A, Ohta A, Maeda Y, Yamada K, Tao K, Saito R | Infect Drug Resist | 10.2147/IDR.S181714 | 2018 |
Reference
@id | authors | catalogue | doi/url | title | journal | pubmed |
---|---|---|---|---|---|---|
3484 | Curators of the DSMZ | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 9143) | https://www.dsmz.de/collection/catalogue/details/culture/DSM-9143 | |||
20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M. | 10.1099/ijsem.0.004332 | List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ | |||
20218 | Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P. | 10.1016/j.syapm.2013.11.002 | StrainInfo introduces electronic passports for microorganisms. | Syst Appl Microbiol. 37: 42-50 2014 | 24321274 | |
37341 | Curators of the CIP | https://brclims.pasteur.fr/brcWeb/souche/detail/1/10674 | ||||
44172 | Curators of the CCUG | Culture Collection University of Gothenburg (CCUG) (CCUG 353) | https://www.ccug.se/strain?id=353 | |||
66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann | https://diaspora-project.de/progress.html#genomes | Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) | |||
68377 | Automatically annotated from API NH | |||||
68382 | Automatically annotated from API zym | |||||
69479 | João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich. | https://microbeatlas.org/ | MicrobeAtlas 1.0 beta | |||
77565 | Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J. | 10.60712/SI-ID3590.1 | StrainInfo: A central database for resolving microbial strain identifiers | |||
117212 | Curators of the CIP | Collection of Institut Pasteur (CIP 73.21) | https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%2073.21 |