Strain identifier

BacDive ID: 8161

Type strain: Yes

Species: Moraxella catarrhalis

Strain Designation: Ne 11, Nell

Strain history: CIP <- 1973, ATCC <- B.W. Catlin: strain Ne 11 <- Rochester Univ.

NCBI tax ID(s): 480 (species)

For citation purpose refer to the digital object identifier (doi) of the current version.
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General

@ref: 3484

BacDive-ID: 8161

DSM-Number: 9143

keywords: genome sequence, 16S sequence, Bacteria, obligate aerobe, mesophilic, Gram-negative, coccus-shaped, human pathogen

description: Moraxella catarrhalis Ne 11 is an obligate aerobe, mesophilic, Gram-negative human pathogen of the family Moraxellaceae.

NCBI tax id

  • NCBI tax id: 480
  • Matching level: species

strain history

@refhistory
3484<- ATCC; ATCC 25238 <- B. W. Catlin; <- University of Rochester, New York; Nell
117212CIP <- 1973, ATCC <- B.W. Catlin: strain Ne 11 <- Rochester Univ.

doi: 10.13145/bacdive8161.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Pseudomonadales
  • family: Moraxellaceae
  • genus: Moraxella
  • species: Moraxella catarrhalis
  • full scientific name: Moraxella catarrhalis (Frosch and Kolle 1896) Henriksen and Bøvre 1968 (Approved Lists 1980)
  • synonyms

    @refsynonym
    20215Branhamella catarrhalis
    20215Mikrokokkus catarrhalis

@ref: 3484

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Pseudomonadales

family: Moraxellaceae

genus: Moraxella

species: Moraxella catarrhalis

full scientific name: Moraxella catarrhalis (Frosch and Kolle 1896) Henriksen and Bøvre 1968

strain designation: Ne 11, Nell

type strain: yes

Morphology

cell morphology

  • @ref: 117212
  • gram stain: negative
  • cell shape: coccus-shaped
  • motility: no

colony morphology

@reftype of hemolysisincubation period
3484gamma1-2 days
117212

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
3484COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)yeshttps://mediadive.dsmz.de/medium/693Name: COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) Composition: Defibrinated sheep blood 50.0 g/l Columbia agar base
3484BHI MEDIUM (DSMZ Medium 215)yeshttps://mediadive.dsmz.de/medium/215Name: BHI MEDIUM (DSMZ Medium 215) Composition: Brain heart infusion 37.0 g/l Distilled water
37341MEDIUM 72- for trypto casein soja agaryesDistilled water make up to (1000.000 ml);Trypto casein soy agar (40.000 g)
117212CIP Medium 72yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=72

culture temp

@refgrowthtypetemperaturerange
3484positivegrowth37mesophilic
37341positivegrowth30mesophilic
117212positivegrowth30-37mesophilic
117212nogrowth25mesophilic
117212nogrowth41thermophilic

Physiology and metabolism

oxygen tolerance

  • @ref: 117212
  • oxygen tolerance: obligate aerobe

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
1172124853esculin-hydrolysis
117212606565hippurate+hydrolysis
11721217632nitrate-builds gas from
11721217632nitrate+reduction
11721216301nitrite-builds gas from
11721216301nitrite-reduction
11721217632nitrate-respiration
6837717634D-glucose-builds acid from
6837715824D-fructose-builds acid from
6837717306maltose-builds acid from
6837717992sucrose-builds acid from
6837718257ornithine-degradation
6837727897tryptophan-energy source

metabolite production

@refChebi-IDmetaboliteproduction
6837735581indoleno
11721235581indoleno
117212polysaccharidesno

metabolite tests

  • @ref: 68377
  • Chebi-ID: 35581
  • metabolite: indole
  • indole test: -

enzymes

@refvalueactivityec
3484catalase+1.11.1.6
3484cytochrome-c oxidase+1.9.3.1
68377tryptophan deaminase-4.1.99.1
68377proline-arylamidase-3.4.11.5
68377beta-galactosidase-3.2.1.23
68377ornithine decarboxylase-4.1.1.17
68377beta-lactamase-3.5.2.6
117212oxidase+
117212alcohol dehydrogenase-1.1.1.1
117212gelatinase-
117212amylase-
117212DNase+
117212catalase+1.11.1.6
117212tween esterase-
117212gamma-glutamyltransferase-2.3.2.2
117212lysine decarboxylase-4.1.1.18
117212ornithine decarboxylase-4.1.1.17
117212urease-3.5.1.5
68382alkaline phosphatase+3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)+
68382lipase (C 14)+
68382leucine arylamidase+3.4.11.1
68382valine arylamidase+
68382cystine arylamidase-3.4.11.3
68382trypsin-3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase-3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase-3.2.1.20
68382beta-glucosidase-3.2.1.21
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

fatty acid profile

  • fatty acids

    @reffatty acidpercentageECL
    44172C10:015.810
    44172C12:08.812
    44172C16:04.816
    44172C12:0 3OH1413.455
    44172C16:1 ω7c9.515.819
    44172C17:1 ω8c2.916.792
    44172C18:1 ω9c26.417.769
    44172C18:2 ω6,9c/C18:0 ANTE11.717.724
    44172Unidentified2.610.241
    44172Unidentified3.618.095
  • type of FA analysis: whole cell analysis
  • method/protocol: CCUG

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
117212-++++++---++--------

API NH

@refPENGLUFRUMALSACODCURELIPPALbeta GALProAGGTIND
3484-------+-----
44172-------+-----
3484------+------
3484------+------
3484-------++----
3484-------+-----
3484-------+-----
3484-------++/---+-

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
117212---------------------------------------------------------------------------------------------------

Isolation, sampling and environmental information

isolation

  • @ref: 3484
  • geographic location: NY, Rochester
  • country: USA
  • origin.country: USA
  • continent: North America

taxonmaps

  • @ref: 69479
  • File name: preview.99_650.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_80;96_246;97_268;98_298;99_650&stattab=map
  • Last taxonomy: Moraxella
  • 16S sequence: U10876
  • Sequence Identity:
  • Total samples: 27444
  • soil counts: 986
  • aquatic counts: 3037
  • animal counts: 23016
  • plant counts: 405

Safety information

risk assessment

@refpathogenicity humanpathogenicity animalbiosafety levelbiosafety level comment
3484yesyes2Risk group (German classification)
1172121Risk group (French classification)

Sequence information

16S sequences

@refdescriptionaccessionlengthdatabaseNCBI tax ID
20218Moraxella catarrhalis 16S rRNA gene, partialAJ247218447ena480
20218Moraxella catarrhalis ATCC 25238 16S rRNA geneU108761511ena480
3484Moraxella catarrhalis 16S ribosomal RNA gene, partial sequenceAF0051851446ena480

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Moraxella catarrhalis NCTC11020GCA_900476075completencbi480
66792Moraxella catarrhalis CCUG 353GCA_001632285scaffoldncbi480
66792Moraxella catarrhalis strain CCUG 353480.224wgspatric480
66792Moraxella catarrhalis strain NCTC11020480.283completepatric480
66792Moraxella catarrhalis CCUG 3532744054833draftimg480
66792Moraxella catarrhalis NCTC 110202847058841completeimg480

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
flagellatedno94.762no
gram-positiveno97.664no
anaerobicno99.356no
aerobicno85.334no
halophileno78.328no
spore-formingno97.466no
thermophileno98.189yes
glucose-utilno77.549no
motileno92.41no
glucose-fermentno93.16no

External links

@ref: 3484

culture collection no.: CCUG 353, NCTC 11020, LMG 11192, DSM 9143, ATCC 25238, CIP 73.21

straininfo link

  • @ref: 77565
  • straininfo: 3590

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Enzymology234507Enzyme electrophoretograms in the analysis of taxon relatedness of Micrococcus cryophilus, Branhamella catarrhalis and atypical Neisserias.Fox RH, McClain DEJ Gen Microbiol10.1099/00221287-86-2-2101975Acetates/metabolism, Butyrates/metabolism, Cell-Free System, Electrophoresis, Polyacrylamide Gel, Esterases/metabolism, Isocitrate Dehydrogenase/metabolism, Isoenzymes/metabolism, Malate Dehydrogenase/metabolism, Micrococcus/*classification/enzymology, NADP/metabolism, Neisseria/*classification/enzymology, Neisseriaceae/*classification/enzymology, Propionates/metabolism, Species Specificity, SpectrophotometryMetabolism
Enzymology1592697Permeability of the outer membrane of Moraxella catarrhalis for beta-lactam antibiotics.Gotoh N, Tanaka S, Nishino TJ Antimicrob Chemother10.1093/jac/29.3.2791992Anti-Bacterial Agents/*pharmacokinetics, Cell Membrane/metabolism, Cell Membrane Permeability, Diffusion, Kinetics, Liposomes/metabolism, Microbial Sensitivity Tests, Moraxella catarrhalis/*metabolism/ultrastructure, beta-LactamsMetabolism
Enzymology2514110Esterase electrophoresis: a molecular tool for studying the epidemiology of Branhamella catarrhalis nosocomial infection.Picard B, Goullet P, Denamur E, Suermondt GEpidemiol Infect10.1017/s09502688000309461989Adult, Bacterial Infections/epidemiology/*microbiology, Child, Cross Infection/epidemiology/*microbiology, *Disease Outbreaks, Electrophoresis, Esterases/*analysis, Humans, Infant, Newborn, Moraxella catarrhalis/*classification/enzymology, Respiratory Tract Infections/epidemiology/microbiologyPathogenicity
Enzymology7516823Structural studies of the O-polysaccharide from the lipopolysaccharide of Moraxella (Branhamella) catarrhalis serotype A (strain ATCC 25238).Edebrink P, Jansson PE, Rahman MM, Widmalm G, Holme T, Rahman M, Weintraub ACarbohydr Res10.1016/0008-6215(94)80040-51994Carbohydrate Conformation, Carbohydrate Sequence, Electrophoresis, Polyacrylamide Gel, Glucosamine/analysis, Indicators and Reagents, Magnetic Resonance Spectroscopy, Mass Spectrometry, Methylation, Molecular Sequence Data, Moraxella catarrhalis/*chemistry/classification/immunology, O Antigens, Oligosaccharides/chemistry/isolation & purification, Polysaccharides, Bacterial/*chemistry/isolation & purification, Serotyping, Sugar Acids/analysisPhylogeny
Enzymology7898388Restriction fragment length polymorphism (RFLP) of genomic DNA of Moraxella (Branhamella) catarrhalis isolates in a hospital.Kawakami Y, Ueno I, Katsuyama T, Furihata K, Matsumoto HMicrobiol Immunol10.1111/j.1348-0421.1994.tb02142.x1994Electrophoresis, Gel, Pulsed-Field, Genome, Bacterial, Humans, Molecular Epidemiology/methods, Moraxella catarrhalis/*classification, Neisseriaceae Infections/microbiology, *Polymorphism, Restriction Fragment LengthGenetics
Genetics8119289Characterization of the lipopolysaccharide of Moraxella catarrhalis. Structural analysis of the lipid A from M. catarrhalis serotype A lipopolysaccharide.Masoud H, Perry MB, Richards JCEur J Biochem10.1111/j.1432-1033.1994.tb18616.x1994Carbohydrate Sequence, Fatty Acids/analysis, Hydroxylation, Lipid A/*chemistry, Magnetic Resonance Spectroscopy, Molecular Sequence Data, Molecular Structure, Moraxella catarrhalis/*chemistry/classification, Phosphates/chemistry, Serotyping, Spectrometry, Mass, Fast Atom BombardmentPhylogeny
Genetics8577264Construction of a combined NotI/SmaI physical and genetic map of Moraxella (Branhamella) catarrhalis strain ATCC25238.Furihata K, Sato K, Matsumoto HMicrobiol Immunol10.1111/j.1348-0421.1995.tb03266.x1995Amino Acid Sequence, Base Composition, Cloning, Molecular, DNA, Bacterial/isolation & purification, *Deoxyribonucleases, Type II Site-Specific, Gene Library, Genes, Bacterial, *Genome, Bacterial, Humans, Molecular Sequence Data, Molecular Weight, Moraxella catarrhalis/*genetics, *Restriction MappingEnzymology
9573066Synthesis and characterization of lipooligosaccharide-based conjugates as vaccine candidates for Moraxella (Branhamella) catarrhalis.Gu XX, Chen J, Barenkamp SJ, Robbins JB, Tsai CM, Lim DJ, Battey JInfect Immun10.1128/IAI.66.5.1891-1897.19981998Animals, Antibodies, Bacterial/blood, Bacterial Vaccines/*immunology, Female, Immune Sera/immunology, Lipopolysaccharides/*immunology, Mice, Moraxella catarrhalis/*immunology, Rabbits, Tetanus Toxoid/immunology, Vaccines, Conjugate/immunology, Vaccines, Synthetic/*immunology
Genetics10383971Characterization of the Moraxella catarrhalis uspA1 and uspA2 genes and their encoded products.Cope LD, Lafontaine ER, Slaughter CA, Hasemann CA Jr, Aebi C, Henderson FW, McCracken GH Jr, Hansen EJJ Bacteriol10.1128/JB.181.13.4026-4034.19991999Amino Acid Sequence, Antigens, Bacterial/biosynthesis/*genetics, Antigens, Surface/biosynthesis/*genetics, Bacterial Outer Membrane Proteins/biosynthesis/*genetics, Base Sequence, Gene Expression, *Genes, Bacterial, Mass Spectrometry, Molecular Sequence Data, Molecular Weight, Moraxella catarrhalis/*genetics, Protein Biosynthesis, Repetitive Sequences, Amino Acid, Sequence Analysis, Sequence Homology, Amino Acid, Species Specificity, Transcription, GeneticTranscriptome
Enzymology10420581Construction of a genomic map of Moraxella (Branhamella) catarrhalis ATCC 25238 and physical mapping of virulence-associated genes.Nguyen KT, Hansen EJ, Farinha MACan J Microbiol1999Blotting, Southern, *Chromosome Mapping, Deoxyribonucleases, Type II Site-Specific/metabolism, Electrophoresis, Gel, Pulsed-Field, *Genes, Bacterial, Moraxella catarrhalis/*genetics/*pathogenicity, Oligonucleotide Probes, RNA, Ribosomal, 23S/genetics, Restriction Mapping, Virulence/genetics, rRNA OperonMetabolism
Pathogenicity22703292Antimicrobial and antioxidant properties of Betula aetnensis Rafin. (Betulaceae) leaves extract.Acquaviva R, Menichini F, Ragusa S, Genovese C, Amodeo A, Tundis R, Loizzo MR, Iauk LNat Prod Res10.1080/14786419.2012.6953642012Anti-Bacterial Agents/*chemistry/pharmacology, Antioxidants/*chemistry, Betula/*chemistry, Haemophilus influenzae/drug effects, Microbial Sensitivity Tests, Moraxella catarrhalis/drug effects, Plant Extracts/*chemistry/pharmacology, Plant Leaves/*chemistry
Genetics27313296Draft Genome Sequence of Moraxella catarrhalis Type Strain CCUG 353T.Jakobsson HE, Salva-Serra F, Thorell K, Gonzales-Siles L, Boulund F, Karlsson R, Sikora P, Engstrand L, Kristiansson E, Moore ERGenome Announc10.1128/genomeA.00552-162016
30464556Novel mechanism responsible for high-level macrolide resistance in Moraxella catarrhalis.Kasai A, Ohta A, Maeda Y, Yamada K, Tao K, Saito RInfect Drug Resist10.2147/IDR.S1817142018

Reference

@idauthorscataloguedoi/urltitlejournalpubmed
3484Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 9143)https://www.dsmz.de/collection/catalogue/details/culture/DSM-9143
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
20218Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.10.1016/j.syapm.2013.11.002StrainInfo introduces electronic passports for microorganisms.Syst Appl Microbiol. 37: 42-50 201424321274
37341Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/10674
44172Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 353)https://www.ccug.se/strain?id=353
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
68377Automatically annotated from API NH
68382Automatically annotated from API zym
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
77565Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID3590.1StrainInfo: A central database for resolving microbial strain identifiers
117212Curators of the CIPCollection of Institut Pasteur (CIP 73.21)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%2073.21