Strain identifier

BacDive ID: 8153

Type strain: Yes

Species: Moraxella bovis

Strain Designation: L-3

Strain history: CIP <- 1970, M. Piéchaud, Inst. Pasteur, Paris, France <- ATCC, Moraxella bovis <- R.D. Barmer: strain KSC 494

NCBI tax ID(s): 476 (species)

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General

@ref: 2595

BacDive-ID: 8153

DSM-Number: 6328

keywords: genome sequence, 16S sequence, Bacteria, microaerophile, mesophilic, Gram-negative, rod-shaped, human pathogen

description: Moraxella bovis L-3 is a microaerophile, mesophilic, Gram-negative human pathogen that was isolated from cattle with pinkeye.

NCBI tax id

  • NCBI tax id: 476
  • Matching level: species

strain history

@refhistory
2595<- ATCC <- R.D. Barner; KSC 494
116913CIP <- 1970, M. Piéchaud, Inst. Pasteur, Paris, France <- ATCC, Moraxella bovis <- R.D. Barmer: strain KSC 494

doi: 10.13145/bacdive8153.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Pseudomonadales
  • family: Moraxellaceae
  • genus: Moraxella
  • species: Moraxella bovis
  • full scientific name: Moraxella bovis (Hauduroy et al. 1937) Murray 1948 (Approved Lists 1980)

  • synonyms

    • @ref: 20215
    • synonym: Haemophilus bovis

@ref: 2595

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Pseudomonadales

family: Moraxellaceae

genus: Moraxella

species: Moraxella bovis

full scientific name: Moraxella bovis (Hauduroy et al. 1937) Murray 1948

strain designation: L-3

type strain: yes

Morphology

cell morphology

  • @ref: 116913
  • gram stain: negative
  • cell shape: rod-shaped
  • motility: no

colony morphology

@reftype of hemolysishemolysis abilityincubation period
2595beta11-2 days
1169131

Culture and growth conditions

culture medium

@refnamegrowthlinkcomposition
2595BHI MEDIUM (DSMZ Medium 215)yeshttps://mediadive.dsmz.de/medium/215Name: BHI MEDIUM (DSMZ Medium 215) Composition: Brain heart infusion 37.0 g/l Distilled water
2595COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)yeshttps://mediadive.dsmz.de/medium/693Name: COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) Composition: Defibrinated sheep blood 50.0 g/l Columbia agar base
37705MEDIUM 6 - Columbia agar with 10 % horse bloodyesDistilled water make up to (1000.000 ml);Columbia agar (39.000 g);Horseblood (100.000 ml)
116913CIP Medium 6yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=6

culture temp

@refgrowthtypetemperaturerange
2595positivegrowth30mesophilic
37705positivegrowth30mesophilic
44492positivegrowth37mesophilic
116913positivegrowth30mesophilic

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
44492microaerophile
116913obligate aerobe

compound production

@refcompound
2595restriction endonuclease MboI
2595MboII methylase

halophily

@refsaltgrowthtested relationconcentration
116913NaClpositivegrowth0-4 %
116913NaClnogrowth6 %
116913NaClnogrowth8 %
116913NaClnogrowth10 %

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
6836925115malate-assimilation
6836917128adipate-assimilation
6836927689decanoate-assimilation
6836924265gluconate-assimilation
6836917306maltose-assimilation
6836959640N-acetylglucosamine-assimilation
6836916899D-mannitol-assimilation
6836916024D-mannose-assimilation
6836930849L-arabinose-assimilation
6836917634D-glucose-assimilation
683695291gelatin+hydrolysis
683694853esculin-hydrolysis
6836916199urea-hydrolysis
6836929016arginine-hydrolysis
6836917634D-glucose-fermentation
6836927897tryptophan-energy source
6836917632nitrate-reduction
6836830849L-arabinose-fermentation
6836827613amygdalin-fermentation
6836828053melibiose-fermentation
6836817992sucrose-fermentation
6836862345L-rhamnose-fermentation
6836830911sorbitol-fermentation
6836817268myo-inositol-fermentation
6836816899D-mannitol-fermentation
6836817634D-glucose-fermentation
683685291gelatin-hydrolysis
6836827897tryptophan-energy source
6836816199urea-hydrolysis
6836816947citrate-assimilation
6836818257ornithine-degradation
6836825094lysine-degradation
6836829016arginine-hydrolysis
11691316947citrate-carbon source
11691317632nitrate-reduction
11691316301nitrite-reduction
11691317632nitrate-respiration
6837715824D-fructose-builds acid from
6837717306maltose-builds acid from
6837717992sucrose-builds acid from
6837718257ornithine-degradation
6837727897tryptophan-energy source

antibiotic resistance

  • @ref: 116913
  • metabolite: 0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)
  • is antibiotic: yes
  • is sensitive: yes
  • is resistant: no

metabolite production

@refChebi-IDmetaboliteproduction
6837735581indoleno
6836935581indoleno
6836815688acetoinno
6836835581indoleno
6836816136hydrogen sulfideno
11691335581indoleno

metabolite tests

@refChebi-IDmetaboliteindole testvoges-proskauer-test
6837735581indole-
6836935581indole-
6836815688acetoin-
6836835581indole-

enzymes

@refvalueactivityec
2595catalase-1.11.1.6
2595cytochrome-c oxidase+1.9.3.1
68377tryptophan deaminase-4.1.99.1
68377beta-galactosidase-3.2.1.23
68377alkaline phosphatase-3.1.3.1
68377ornithine decarboxylase-4.1.1.17
68369cytochrome oxidase-1.9.3.1
68369gelatinase+
68369beta-glucosidase-3.2.1.21
68369urease-3.5.1.5
68369arginine dihydrolase-3.5.3.6
68368cytochrome oxidase+1.9.3.1
68368gelatinase-
68368tryptophan deaminase-4.1.99.1
68368urease-3.5.1.5
68368ornithine decarboxylase-4.1.1.17
68368lysine decarboxylase-4.1.1.18
68368arginine dihydrolase-3.5.3.6
68368beta-galactosidase-3.2.1.23
116913oxidase+
116913gelatinase+/-
116913catalase+1.11.1.6
116913lecithinase+
116913lipase+
116913tryptophan deaminase-
116913urease-3.5.1.5

API 20E

@refONPGADH ArgLDC LysODCCITH2SURETDA TrpINDVPGELGLUMANINOSorRHASACMELAMYARAOX
2595--------------------+

API 20NE

@refNO3TRPGLU_ FermADH ArgUREESCGELPNPGGLU_ AssimARAMNEMANNAGMALGNTCAPADIMLTCITPACOX
2595------+--------------

API NH

@refPENGLUFRUMALSACODCURELIPPALbeta GALProAGGTIND
2595+/------+---+--
2595-+/------+---+-

API biotype100

@refGLUFRUGALTREMNESBEMELSACRAFMTEMALLACLTEMbGaMaGaCELGENMbGuESCRIBARAXYLPLERHAFUCMLZDARLLARLXLTDULTAGGLYINOMANMTLTURSORADOHBGLYXERYMDG3MDGSATMUCLTATDTATMTATDMLTLMLTCATETATETTECITGRTGAT2KG5KGTRYNAGGNTPACPATpOBEQATGTEmOBEBATPPATCMTTGEBETPCEABTHINLATCAPCYTHISSUCFUMGREGYTAVTETNTTNGLNITA3OBUAPTGTTPRODALALALASERMNTPROPTYR2KT
116913---------------------------------------------------------------------------------------------------

Isolation, sampling and environmental information

isolation

@refsample type
2595cattle with pinkeye
44492Cattle,pinkeye
116913Animal, Cattle with pinkeye

isolation source categories

Cat1Cat2Cat3
#Infection#Inflammation
#Host#Mammals#Bovinae (Cow, Cattle)

taxonmaps

  • @ref: 69479
  • File name: preview.99_2594.png
  • url: https://microbeatlas.org/index.html?action=taxon&taxon_id=90_80;96_246;97_1653;98_2009;99_2594&stattab=map
  • Last taxonomy: Moraxella bovis subclade
  • 16S sequence: AF005182
  • Sequence Identity:
  • Total samples: 686
  • soil counts: 3
  • aquatic counts: 192
  • animal counts: 490
  • plant counts: 1

Safety information

risk assessment

@refpathogenicity humanpathogenicity animalbiosafety levelbiosafety level comment
2595yesyes2Risk group (German classification)
1169131Risk group (French classification)

Sequence information

16S sequences

  • @ref: 2595
  • description: Moraxella bovis 16S ribosomal RNA gene, partial sequence
  • accession: AF005182
  • length: 1447
  • database: ena
  • NCBI tax ID: 476

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Moraxella bovis CCUG 2133GCA_002014975scaffoldncbi476
66792Moraxella bovis strain ATCC 10900476.23plasmidpatric476
66792Moraxella bovis strain CCUG 2133476.10wgspatric476
66792Moraxella bovis CCUG 21332868718361draftimg476

GC content

  • @ref: 2595
  • GC-content: 42.5

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
flagellatedno93.012no
gram-positiveno97.172no
anaerobicno96.826no
aerobicno87.19yes
halophileno72.195no
spore-formingno96.588no
glucose-utilno80.203no
thermophileno98.335yes
motileno87.82no
glucose-fermentno91.192no

External links

@ref: 2595

culture collection no.: DSM 6328, ATCC 10900, CDC KC745, IMET 11301, JCM 17254, CCUG 2133, NCTC 11013, LMG 986, NCDC KC 745, CIP 70.40, IAM 12313, LMG 5299, KSC 494

straininfo link

  • @ref: 77557
  • straininfo: 3594

literature

topicPubmed-IDtitleauthorsjournalyearmeshtopic2DOI
Enzymology2301819Biological reactivity of Moraxella bovis lipopolysaccharide.Johansen KA, Wannemuehler MJ, Rosenbusch RFAm J Vet Res1990Electrophoresis, Polyacrylamide Gel, Fever/chemically induced, Lipopolysaccharides/*pharmacology, *Moraxella/pathogenicity, Shwartzman Phenomenon, Species Specificity, Tumor Necrosis Factor-alpha/biosynthesisPhylogeny
2865080Serologic and protective characterization of Moraxella bovis pili.Lehr C, Jayappa HG, Goodnow RACornell Vet1985Animals, Cattle, Cattle Diseases/immunology/*prevention & control, Cross Reactions, Fimbriae, Bacterial/*immunology, Keratoconjunctivitis/immunology/prevention & control/*veterinary, Microscopy, Electron, Moraxella/*immunology/ultrastructure, Serology, Vaccines/immunology
Metabolism19332813M1.MboII and M2.MboII type IIS methyltransferases: different specificities, the same target.Furmanek-Blaszk B, Boratynski R, Zolcinska N, Sektas MMicrobiology (Reading)2009Adenine/analogs & derivatives/*metabolism, Amino Acid Sequence, Animals, Cattle, Cytosine/analogs & derivatives/*metabolism, DNA Methylation, DNA Restriction-Modification Enzymes/chemistry/genetics/metabolism, DNA, Bacterial/chemistry/metabolism, DNA, Single-Stranded/chemistry/metabolism, Molecular Sequence Data, Moraxella bovis/*enzymology, Site-Specific DNA-Methyltransferase (Adenine-Specific)/chemistry/genetics/*metabolism, *Substrate SpecificityEnzymology10.1099/mic.0.025023-0
Metabolism23129346Molecular characterization of plasmid pMbo4.6 of Moraxella bovis ATCC 10900.Furmanek-Blaszk B, Kurpiewska N, Boratynski R, Sektas MCurr Microbiol2012Amino Acid Sequence, Bacterial Proteins/chemistry/genetics/metabolism, Base Sequence, DNA-Binding Proteins/chemistry/genetics/metabolism, Gene Expression Regulation, Bacterial, Gene Order, Molecular Sequence Data, Moraxella bovis/*genetics/metabolism, Plasmids/*genetics, Promoter Regions, Genetic, Protein Binding, Replication Origin, Sequence Alignment, Sequence Analysis, DNA, Transcription, GeneticGenetics10.1007/s00284-012-0257-6
Phylogeny36067072Moraxella nasovis sp. nov., isolated from a sheep with respiratory disease.Li F, Zhu P, Li Z, Zhao W, Gao H, Hong Q, Song J, Yang SInt J Syst Evol Microbiol2022Animals, Bacterial Typing Techniques, Base Composition, China, DNA, Bacterial/genetics, *Fatty Acids/chemistry, Moraxella/genetics, Phospholipids/chemistry, Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, Sheep, *Sodium Chloride, Ubiquinone/chemistryTranscriptome10.1099/ijsem.0.005511

Reference

@idauthorscataloguedoi/urltitle
2595Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 6328)https://www.dsmz.de/collection/catalogue/details/culture/DSM-6328
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
37705Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/10511
44492Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 2133)https://www.ccug.se/strain?id=2133
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
68368Automatically annotated from API 20E
68369Automatically annotated from API 20NE
68377Automatically annotated from API NH
69479João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.https://microbeatlas.org/MicrobeAtlas 1.0 beta
77557Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID3594.1StrainInfo: A central database for resolving microbial strain identifiers
116913Curators of the CIPCollection of Institut Pasteur (CIP 70.40)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%2070.40