Strain identifier

BacDive ID: 8148

Type strain: Yes

Species: Acinetobacter indicus

Strain Designation: A648

Strain history: CIP <- 2012, DSMZ <- Delhi Univ., Delhi, India: strain A648

NCBI tax ID(s): 1341679 (strain), 756892 (species)

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General

@ref: 18025

BacDive-ID: 8148

DSM-Number: 25388

keywords: genome sequence, 16S sequence, Bacteria, aerobe, Gram-negative, ovoid-shaped

description: Acinetobacter indicus A648 is an aerobe, Gram-negative, ovoid-shaped bacterium that was isolated from hexachlorocyclohexane dump site.

NCBI tax id

NCBI tax idMatching level
756892species
1341679strain

strain history

@refhistory
18025<- R. Lal, Univ. Delhi, Dept. Zoology, India; A648
120085CIP <- 2012, DSMZ <- Delhi Univ., Delhi, India: strain A648

doi: 10.13145/bacdive8148.20230509.8.1

Name and taxonomic classification

LPSN

  • @ref: 20215
  • description: domain/bacteria
  • keyword: phylum/pseudomonadota
  • domain: Bacteria
  • phylum: Pseudomonadota
  • class: Gammaproteobacteria
  • order: Pseudomonadales
  • family: Moraxellaceae
  • genus: Acinetobacter
  • species: Acinetobacter indicus
  • full scientific name: Acinetobacter indicus Malhotra et al. 2012

  • synonyms

    • @ref: 20215
    • synonym: Acinetobacter guangdongensis

@ref: 18025

domain: Bacteria

phylum: Proteobacteria

class: Gammaproteobacteria

order: Pseudomonadales

family: Moraxellaceae

genus: Acinetobacter

species: Acinetobacter indicus

full scientific name: Acinetobacter indicus Malhotra et al. 2012

strain designation: A648

type strain: yes

Morphology

cell morphology

@refgram staincell widthcell shapemotilityconfidence
30511negative1 µmovoid-shapedno
69480no96.768
69480negative99.971
120085negativeoval-shapedno

colony morphology

  • @ref: 18025
  • type of hemolysis: gamma
  • incubation period: 2-3 days

pigmentation

  • @ref: 30511
  • production: yes

Culture and growth conditions

culture medium

@refnamegrowthcompositionlink
42124MEDIUM 368 - for Luria bertaniyesDistilled water make up to (1000.000 ml);Sodium chloride (10.000 g);Agar(15.000 g);Yeast extract (5.000 g);Tryptone (10.000 g)
120085CIP Medium 368yeshttps://catalogue-crbip.pasteur.fr/fiche_milieu.xhtml?crbip=368

culture temp

@refgrowthtypetemperaturerange
30511positivegrowth22-42
30511positiveoptimum28mesophilic
42124positivegrowth30mesophilic
63652positivegrowth30mesophilic

culture pH

@refabilitytypepHPH range
30511positivegrowth07-09alkaliphile
30511positiveoptimum7

Physiology and metabolism

oxygen tolerance

@refoxygen tolerance
30511aerobe
63652aerobe
120085facultative anaerobe

spore formation

@refspore formationconfidence
69481no100
69480no99.998

halophily

@refsaltgrowthtested relationconcentration
30511NaClpositivegrowth0-5 %
30511NaClpositiveoptimum1 %

metabolite utilization

@refChebi-IDmetaboliteutilization activitykind of utilization tested
3051130089acetate+carbon source
3051116236ethanol+carbon source
3051117632nitrate+reduction
68371Potassium 5-ketogluconate-builds acid from
68371Potassium 2-ketogluconate-builds acid from
6837124265gluconate+builds acid from
6837118403L-arabitol-builds acid from
6837118333D-arabitol-builds acid from
6837118287L-fucose-builds acid from
6837128847D-fucose-builds acid from
6837116443D-tagatose+builds acid from
6837162318D-lyxose-builds acid from
6837132528turanose-builds acid from
6837128066gentiobiose+builds acid from
6837117151xylitol-builds acid from
6837128087glycogen-builds acid from
6837116634raffinose-builds acid from
683716731melezitose+builds acid from
6837115443inulin-builds acid from
6837127082trehalose+builds acid from
6837117992sucrose+builds acid from
6837128053melibiose-builds acid from
6837117716lactose+builds acid from
6837117306maltose+builds acid from
6837117057cellobiose+builds acid from
6837117814salicin+builds acid from
683714853esculin+builds acid from
6837118305arbutin+builds acid from
6837127613amygdalin+builds acid from
6837159640N-acetylglucosamine+builds acid from
68371320061methyl alpha-D-glucopyranoside-builds acid from
6837143943methyl alpha-D-mannoside-builds acid from
6837117924D-sorbitol+builds acid from
6837116899D-mannitol+builds acid from
6837117268myo-inositol-builds acid from
6837116813galactitol-builds acid from
6837162345L-rhamnose-builds acid from
6837117266L-sorbose-builds acid from
6837116024D-mannose+builds acid from
6837115824D-fructose+builds acid from
6837117634D-glucose+builds acid from
6837112936D-galactose+builds acid from
6837174863methyl beta-D-xylopyranoside-builds acid from
6837115963ribitol-builds acid from
6837165328L-xylose-builds acid from
6837165327D-xylose-builds acid from
6837116988D-ribose+builds acid from
6837130849L-arabinose-builds acid from
6837117108D-arabinose-builds acid from
6837117113erythritol-builds acid from
6837117754glycerol+builds acid from
12008517632nitrate+reduction
12008516301nitrite-reduction

metabolite production

  • @ref: 120085
  • Chebi-ID: 35581
  • metabolite: indole
  • production: yes

enzymes

@refvalueactivityec
18025catalase+1.11.1.6
18025cytochrome-c oxidase-1.9.3.1
120085oxidase-
120085catalase+1.11.1.6
120085urease+3.5.1.5
68382alkaline phosphatase-3.1.3.1
68382esterase (C 4)+
68382esterase lipase (C 8)+
68382lipase (C 14)-
68382leucine arylamidase+3.4.11.1
68382valine arylamidase-
68382cystine arylamidase+3.4.11.3
68382trypsin-3.4.21.4
68382alpha-chymotrypsin-3.4.21.1
68382acid phosphatase+3.1.3.2
68382naphthol-AS-BI-phosphohydrolase+
68382alpha-galactosidase-3.2.1.22
68382beta-galactosidase-3.2.1.23
68382beta-glucuronidase-3.2.1.31
68382alpha-glucosidase+3.2.1.20
68382beta-glucosidase+3.2.1.21
68382N-acetyl-beta-glucosaminidase-3.2.1.52
68382alpha-mannosidase-3.2.1.24
68382alpha-fucosidase-3.2.1.51

API zym

@refControlAlkaline phosphataseEsteraseEsterase LipaseLipaseLeucine arylamidaseValine arylamidaseCystine arylamidaseTrypsinalpha- ChymotrypsinAcid phosphataseNaphthol-AS-BI-phosphohydrolasealpha- Galactosidasebeta- Galactosidasebeta- Glucuronidasealpha- Glucosidasebeta- GlucosidaseN-acetyl-beta- glucosaminidasealpha- Mannosidasealpha- Fucosidase
120085--++-+-+--++---++---

API 50CHac

@refGLYERYDARALARARIBDXYLLXYLADOMDXGALGLUFRUMNESBERHADULINOMANSORMDMMDGNAGAMYARBESCSALCELMALLACMELSACTREINUMLZRAFAMDGLYGXLTGENTURLYXTAGDFUCLFUCDARLLARLGNT2KG5KG
120085+---+----++++----++--++++++++-++-+-+/---+--+----+--

Isolation, sampling and environmental information

isolation

@refsample typegeographic locationcountryorigin.countrycontinent
18025hexachlorocyclohexane dump siteUttar Pradesh, Lucknow, Ummari villageIndiaINDAsia
63652Hexachlorocyclohexane dump siteVarasaniIndiaINDAsia
120085Environment, Soil samples, HCH dumpsiteLucknowIndiaINDAsia

isolation source categories

  • Cat1: #Engineered
  • Cat2: #Built environment

Safety information

risk assessment

@refbiosafety levelbiosafety level comment
180251Risk group (German classification)
1200851Risk group (French classification)

Sequence information

16S sequences

  • @ref: 18025
  • description: Acinetobacter indicus strain A648 16S ribosomal RNA gene, partial sequence
  • accession: HM047743
  • length: 1393
  • database: ena
  • NCBI tax ID: 1341679

Genome sequences

@refdescriptionaccessionassembly leveldatabaseNCBI tax ID
66792Acinetobacter indicus CIP 110367GCA_000488255scaffoldncbi1341679
66792Acinetobacter indicus CIP 110367 DSM 25388GCA_000830155contigncbi1341679
66792Acinetobacter indicus CIP 1103671341679.3wgspatric1341679
66792Acinetobacter indicus CIP 110367 strain DSM 253881341679.5wgspatric1341679
66792Acinetobacter indicus CIP 1103672579779046draftimg1341679
66792Acinetobacter indicus DSM 253882663763131draftimg1341679

GC content

@refGC-contentmethod
1802540.4thermal denaturation, midpoint method (Tm)
3051140.4

Genome-based predictions

predictions

traitpredictionconfidencetraining_data
spore-formingno100no
motileno92.055yes
flagellatedno96.115yes
gram-positiveno97.499yes
anaerobicno98.378no
aerobicyes92.943no
halophileno76.177no
spore-formingno96.772no
thermophileno99.87yes
glucose-utilno87.823no
glucose-fermentno91.876no

External links

@ref: 18025

culture collection no.: DSM 25388, CCM 7832, CIP 110367, MTCC 10660, CCUG 69702, ANC 4215

straininfo link

  • @ref: 77551
  • straininfo: 382379

literature

topicPubmed-IDtitleauthorsjournalDOIyearmeshtopic2
Phylogeny22247213Acinetobacter indicus sp. nov., isolated from a hexachlorocyclohexane dump site.Malhotra J, Anand S, Jindal S, Rajagopal R, Lal RInt J Syst Evol Microbiol10.1099/ijs.0.037721-02012Acinetobacter/*classification/genetics/isolation & purification, Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fatty Acids/analysis, Hexachlorocyclohexane/*analysis, India, Molecular Sequence Data, Nucleic Acid Hybridization, *Phylogeny, Polyamines/analysis, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA, *Soil Microbiology, Soil Pollutants/analysis, Ubiquinone/analysisGenetics
Phylogeny28884669Acinetobacter guangdongensis Feng et al. 2014 is a junior heterotypic synonym of Acinetobacter indicus Malhotra et al. 2012.Nemec A, Radolfova-Krizova LInt J Syst Evol Microbiol10.1099/ijsem.0.0022512017Acinetobacter/*classification, Bacterial Typing Techniques, DNA, Bacterial/genetics, Genome, Bacterial, Nucleic Acid Hybridization, *Phylogeny, Sequence Analysis, DNAGenetics

Reference

@idauthorscataloguedoi/urltitleID_cross_referencepubmed
18025Curators of the DSMZLeibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSM 25388)https://www.dsmz.de/collection/catalogue/details/culture/DSM-25388
20215Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.10.1099/ijsem.0.004332List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ
30511Barberan A, Caceres Velazquez H, Jones S, Fierer N.10.1128/mSphere.00237-17Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information2684328776041
42124Curators of the CIPhttps://brclims.pasteur.fr/brcWeb/souche/detail/1/8218
63652Curators of the CCUGCulture Collection University of Gothenburg (CCUG) (CCUG 69702)https://www.ccug.se/strain?id=69702
66792Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesAutomatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
68371Automatically annotated from API 50CH acid
68382Automatically annotated from API zym
69480Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmannhttps://diaspora-project.de/progress.html#genomesPredictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information)
69481Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch10.21203/rs.3.rs-2527258/v1Predictions based on the model GenomeNet Sporulation v. 1
77551Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.10.60712/SI-ID382379.1StrainInfo: A central database for resolving microbial strain identifiers
120085Curators of the CIPCollection of Institut Pasteur (CIP 110367)https://catalogue-crbip.pasteur.fr/fiche_catalogue.xhtml?crbip=CIP%20110367